:: Volume 26, Issue 3 (Bimonthly 2022) ::
Feyz 2022, 26(3): 264-272 Back to browse issues page
Investigation the effect of high intensity interval training (HIIT) with curcumin supplementation on mitochondrial cardiomyocyte PGC-1α and ERR-α gene expression in male stroke model rats
Mojdeh Nadaf-Fahmideh , Shahram Gholamrezaei , Ramin Shabani
Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, I.R. Iran. , gholamrezaei@iaurasht.ac.ir
Abstract:   (1191 Views)
Background: Myocardial infarction is an acute condition of myocardial necrosis. So, this study aimed the effect of high intensity interval training with curcumin supplementation on mitochondrial cardiomyocyte PGC-1α and ERR-α gene expression rats.
Materials and Methods: 32 male rats were randomly divided into 4 groups of HIIT, supplement, control and HIIT with supplement training in the two groups of high intensity and HIIT with supplement included performing 8 weeks of 4-minute activity with an intensity of 85-90% VO2max. Curcumin was also used at 15 mg / kg. Mitochondrial PGC-1α and ERR-α gene expression was obtained by real-time PCR. Data were analyzed by ANOVA and Shapiro-Wilk at P≤0.05.
Results: One-way analysis of variance test showed a significant difference in the expression of mitochondrial cardiomyocyte PGC-1α and ERR-α genes in male myocardial infarction mice in the study groups (P=0.001). The results of Tukey test showed a significant increase in PGC-1α and ERR-α gene expression in the HIIT and HIIT groups with supplementation compared to the control and supplement groups (P=0.001). There was no significant difference between the control and complement groups.
Conclusion: The results of this study showed that interval training alone and in interaction with curcumin increases the expression of ERRα and PGC-1α in the heart tissue of male stroke model mice. It seems that interval training and consumption of curcumin can be considered a good way to increase mitochondrial biogenesis and improve heart function.
Keywords: High intensity interval training, Curcumin, PGC-1α, ERR-α
Full-Text [PDF 381 kb]   (738 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/04/27 | Revised: 2023/02/13 | Accepted: 2022/07/4 | Published: 2022/07/30
1. Frigolet ME, Gutiérrez-Aguilar R. The colors of adipose tissue. Gac Med Mex 2020; 156: 142-9.
2. Ultimo S, Zauli G, Martelli AM, Vitale M, McCubrey JA, Capitani S, et al. Cardiovascular disease-related miRNAs expression: potential role as biomarkers and effects of training exercise. Oncotarget 2018; 9(24): 17238.
3. Shalaby RE, Iram S, Oropeza CE, McLachlan A. Peroxisome proliferator-activated receptor γ coactivator family members competitively regulate hepatitis b virus biosynthesis. Virology 2019; 526: 214-21.
4. McKie GL, Wright DC. Biochemical adaptations in white adipose tissue following aerobic exercise: from mitochondrial biogenesis to browning. Biochem J 2020; 477(6): 1061-81.
5. Cantó C, Auwerx J. PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr Opin Lipidol 2009; 20(2): 98.
6. Son YO, Chun JS. Estrogen-related receptor γ is a novel catabolic regulator of osteoarthritis pathogenesis. Bmb Reports 2018; 51(4): 165.
7. Sandri M, Lin J, Handschin C, Yang W, Arany ZP, Lecker SH, et al. PGC-1α protects skeletal muscle from atrophy by suppressing FoxO3 action and atrophy-specific gene transcription. Proc Natl Acad Sci U S A 2006; 103(44): 16260-5.
8. Adhihetty PJ, O'Leary MF, Chabi B, Wicks KL, Hood DA. Effect of denervation on mitochondrially mediated apoptosis in skeletal muscle. J Appl Physiol 2007; 102(3): 1143-51.
9. Hoshino D, Yoshida Y, Kitaoka Y, Hatta H, Bonen A. High-intensity interval training increases intrinsic rates of mitochondrial fatty acid oxidation in rat red and white skeletal muscle. Appl Physiol Nutr Metab 2013; 38(3): 326-33.
10. Kang C, Chung E, Diffee G, Ji LL. Exercise training attenuates aging-associated mitochondrial dysfunction in rat skeletal muscle: role of PGC-1α. Exp Gerontol 2013; 48(11): 1343-50.
11. Wicks KL, Hood DA. Mitochondrial adaptations in denervated muscle: relationship to muscle performance. Am J Physiol 1991; 260(4): C841-C50.
12. Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 2002;7(9):405-10.
13. Youn JY, Zhang J, Zhang Y, Chen H, Liu D, Ping P, et al. Oxidative stress in atrial fibrillation: an emerging role of NADPH oxidase. J Mol Cell Cardiol 2013; 62: 72-9.
14. Bach D, Pich S, Soriano FX, Vega N, Baumgartner B, Oriola J, et al. Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism: a novel regulatory mechanism altered in obesity. J Biol Chem 2003; 278(19): 17190-7.
15. Sacheck JM, Hyatt JPK, Raffaello A, Thomas Jagoe R, Roy RR, Reggie Edgerton V, et al. Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases. FASEB J 2007; 21(1): 1 40-55.
16. Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, et al. The effects of acute and chronic exercise on PGC‐1α, irisin and browning of subcutaneous adipose tissue in humans. FEBS J 2014; 281(3): 739-49.
17. Cox FF, Misiou A, Vierkant A, Ale-Agha N, Grandoch M, Haendeler J, et al. Protective effects of curcumin in cardiovascular diseases—Impact on oxidative stress and mitochondria. Cells 2022; 11(3): 342.
18. Pourbagher-Shahri AM, Farkhondeh T, Ashrafizadeh M, Talebi M, Samargahndian S. Curcumin and cardiovascular diseases: Focus on cellular targets and cascades. Biome Pharma 2021; 136: 111214.
19. Rocha-Rodrigues S, Rodríguez A, Gouveia AM, Gonçalves IO, Becerril S, Ramírez B, et al. Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet. Life Sci 2016; 165: 100-8.
20. Baghaiee B, Bayatmakoo R, Karimi P, Pescatello L. Moderate Aerobic Training Inhibits Middle-Aged Induced Cardiac Calcineurin-NFAT Signaling by Improving TGF-ß, NPR-A, SERCA2, and TRPC6 in Wistar Rats. CELL J (Yakhteh) 2021; 23(7): 756-62.
21. Baghaiee B, Karimi P, Siahkouhian M, Pescatello LS. Moderate aerobic exercise training decreases middle-aged induced pathologic cardiac hypertrophy by improving Klotho expression, MAPK signaling pathway and oxidative stress status in Wistar rats. Iran J Basic Med Sci 2018; 21(9): 911-9.
22. Taheri Gandomani R, Mirzaei B, Damirchi A. The Effect of Moderate and High Intensity Resistance Training on the Expression of PGC-1α, TFAM and AMPK of Cardiomyocytes in Elderly Rats. JAUS 2021; 21(1): 110-2. [in Persian]
23. Langfort J, Viese M, Ploug T, Dela F. Time course of GLUT4 and AMPK protein expression in human skeletal muscle during one month of physical training. Scand J Med Sci Sports 2003; 13(3): 169-74.
24. Cartoni R, Léger B, Hock MB, Praz M, Crettenand A, Pich S, et al. Mitofusins 1/2 and ERRα expression are increased in human skeletal muscle after physical exercise. J Physiol 2005; 567(1): 349-58.
25. Khalafi M, Mohebbi H, Karimi P, Faridnia M, Tabari E. The Effect of High Intensity Interval training and Moderate Intensity Continuous Training on Mitochondrial Content and PGC-1α of Subcutaneous Adipose Tissue in Male Rats with High Fat Diet Induced Obesity. J Sport Bio 2018; 10(3): 297-315.
26. Raoufi a, sirous f, Hoseini A. The Effect of Eight Weeks of Curcumin Supplementation on the Expression of Some Regulatory Genes of Atrophic Processes in the Heart Tissue of Fatty Adult Fatty Rats. JABS 2019; 9(2): 1425-32. [in Persian]
27. Rauofi A, Farsi S, Hosseini SA. Effect of Resistance Training Along with Curcumin Supplementation on Expression of Some Regulator Genes Associated with Cardiac Muscle Structure in Obese Rats. Thrita 2020; 9(2).
28. Mirzaei MR, Gholamrezaei S, Shabani R. The Effect of 8 Weeks of High-Intensity Interval Training (HIIT) with Curcumin Supplementation on Expression of Mitochondrial Cardiomyocyte drp1 and MFN2 Genes in Male Stroke Model Rats. JIMS 2022; 39(652): 914-21. [in Persian]
29. Phillips S, Green H, Tarnopolsky M, Heigenhauser G, Grant S. Progressive effect of endurance training on metabolic adaptations in working skeletal muscle. Am J Physiol 1996;270(2):E265-E72.
30. Yan M, Audet-Walsh É, Manteghi S, Dufour CR, Walker B, Baba M, et al. Chronic AMPK activation via loss of FLCN induces functional beige adipose tissue through PGC-1α/ERRα. Genes Dev 2016; 30(9): 1034-46.
31. Chen P, Wang H, Yang F, Chen H, He W, Wang J. Curcumin promotes osteosarcoma cell death by activating miR‐125a/ERRα signal pathway. J Cell Biochem 2017; 118(1): 74-81.

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