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:: Volume 26, Issue 3 (Bimonthly 2022) ::
Feyz 2022, 26(3): 273-281 Back to browse issues page
The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes
Mehrzad Shabani , Vahid Valipour-Dehnou , Mohammad Reza Tabandeh , Mahdieh Molanouri-Shamsi
Department of Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Khorramabad, I.R. Iran. , valipour.v@lu.ac.ir
Abstract:   (1072 Views)
Background: Heat shock protein 60 (HSP60) is considered as an indicator of mitochondrial stress and plays a role in modulating mitochondrial dysfunction. This study aimed to investigate the effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes.
Materials and Methods: Thirty adult mice were randomly and equally divided into three groups: control (C), diabetic (D) and diabetic-exercise (DE). Diabetes was induced by intraperitoneal injection of Streptozotocin. Aerobic endurance training was performed five sessions per week for eight weeks. 48 hours after the last training session, glucose, insulin and HSP60 levels and insulin resistance index were measured.
Results: Insulin and blood glucose levels and insulin resistance in group DE decreased significantly compared to group D (P<0.05). Also, HSP60 protein levels in group DE increased significantly compared to group D (P<0.05) and decreased significantly in group D compared to group C (P<0.05). Also, the results showed that there was a negative correlation between HSP60 and insulin resistance in all three groups (P= 0.001, r= 0.930).
Conclusion: Diabetes lowers HSP60 levels and aerobic endurance exercise increases its levels. Also, endurance exercise can enhance mitochondrial function in diabetes by increasing HSP60 protein expression. It improves the insulin resistance index, also.
Keywords: Aerobic exercise, Diabetes, Insulin resistance, HSP60, Mice
Full-Text [PDF 398 kb]   (665 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2022/04/9 | Revised: 2022/07/30 | Accepted: 2022/06/27 | Published: 2022/07/30
1. Rowles JE, Keane KN, Gomes Heck T, Cruzat V, Verdile G, Newsholme P. Are heat shock proteins an important link between type 2 diabetes and Alzheimer disease? Int J Mol Med Sci 2020; 21(21): 8204.
2. Fujimaki S, Kuwabara T. Diabetes-induced dysfunction of mitochondria and stem cells in skeletal muscle and the nervous system. Int J Mol Med Sci 2017; 18(10): 2147.
3. Peng TI, Jou MJ. Oxidative stress caused by mitochondrial calcium overload. Ann N Y Acad Sci 2010; 1201(1): 183-8.
4. Taylor R. Insulin resistance and type 2 diabetes. Diabetes 2012; 61(4): 778-9.
5. Voos W, Jaworek W, Wilkening A, Bruderek M. Protein quality control at the mitochondrion. Essays Biochem 2016; 60(2): 213-25.
6. Wondmkun YT. Obesity, insulin resistance, and type 2 diabetes: associations and therapeutic implications. Diabetes Metab Syndr Obes. 2020; 13: 3611.
7. Kim K, Kim YH, Lee SH, Jeon MJ, Park SY, Doh KO. Effect of exercise intensity on unfolded protein response in skeletal muscle of rat. Korean J Physiol Pharmacol 2014; 18(3): 211-6.
8. Cappello F, Gammazza AM, Vilasi S, Ortore MG, San Biagio PL, Campanella C, et al. Chaperonotherapy for Alzheimer’s disease: focusing on HSP60. Heat Shock Protein-Based Therapies 2015: 51-76.
9. Marino Gammazza A, Macaluso F, Di Felice V, Cappello F, Barone R. Hsp60 in skeletal muscle fiber biogenesis and homeostasis: From physical exercise to skeletal muscle pathology. Cells 2018; 7(12): 224.
10. Muchowski PJ, Wacker JL. Modulation of neurodegeneration by molecular chaperones. Nat Rev Neurosci 2005; 6(1): 11-22.
11. Zhang D, Liu H, Zhang Y, Li J, Fu Y, Zheng Y, et al. Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor. Cell Communic Signal 2020; 18(1): 1-9.
12. D’Amico D, Fiore R, Caporossi D, Di Felice VD, Cappello F, Dimauro I, et al. Function and Fiber-Type Specific Distribution of Hsp60 and αB-Crystallin in Skeletal Muscles: Role of Physical Exercise. Biology 2021; 10(2): 77.
13. Wang J, Polaki V, Chen S, Bihl JC. Exercise improves endothelial function associated with alleviated inflammation and oxidative stress of perivascular adipose tissue in type 2 diabetic mice. Oxid Med Cell Longev. 2020; 2020.
14. Ranjbar Kohan N, Tabandeh MR, Nazifi S, Soleimani Z. L‐carnitine improves metabolic disorders and regulates apelin and apelin receptor genes expression in adipose tissue in diabetic rats. Physiologi Rep 2020; 8(23): e14641.
15. Akram S, Tabssum M, Rao M, Qureshi HJ. Effect of endurance exercise on oxidative stress marker malondialdehyde in type 2 diabetic mice. TPMJ 2020; 27(07): 1493-8.
16. Juwono J, Martinus RD. Does Hsp60 provide a link between mitochondrial stress and inflammation in diabetes mellitus? J Diabetes Res 2016; 2016.
17. Bellini S, Barutta F, Mastrocola R, Imperatore L, Bruno G, Gruden G. Heat shock proteins in vascular diabetic complications: review and future perspective. Int J Mol Sci 2017;18(12): 2709.
18. Kleinridders A, Lauritzen HP, Ussar S, Christensen JH, Mori MA, Bross P, et al. Leptin regulation of Hsp60 impacts hypothalamic insulin signaling. JCI 2013; 123(11): 4667-80.
19. Khadir A, Kavalakatt S, Cherian P, Warsame S, Abubaker JA, Dehbi M, et al. Physical exercise enhanced heat shock protein 60 expression and attenuated inflammation in the adipose tissue of human diabetic obese. Front Endocrinol 2018; 9: 16.
20. Lumini JA, Magalhaes J, Oliveira PJ, Ascensao A. Beneficial effects of exercise on muscle mitochondrial function in diabetes mellitus. Sports Med 2008; 38(9): 735-50.
21. Rochette L, Meloux A, Zeller M, Cottin Y, Vergely C. Role of humanin, a mitochondrial-derived peptide, in cardiovascular disorders. Arch Cardiovasc Dis 2020.
22. Rong JX, Qiu Y, Hansen MK, Zhu L, Zhang V, Xie M, et al. Adipose mitochondrial biogenesis is suppressed in db/db and high-fat diet–fed mice and improved by rosiglitazone. Diabetes 2007; 56(7): 1751-60.
23. Achari AE, Jain SK. Adiponectin, a therapeutic target for obesity, diabetes, and endothelial dysfunction. Int J Mol Sci 2017; 18(6): 1321.
24. Noble EG, Milne KJ, Melling CJ. Heat shock proteins and exercise: a primer. Appl Physiol Nutr Metab 2008; 33(5): 1050-75.
25. Sammut IA, Harrison JC. Cardiac mitochondrial complex activity is enhanced by heat shock proteins. Clin Exp Pharmacol Physiol 2003; 30(1‐2): 110-5.
26. Chung J, Nguyen AK, Henstridge DC, Holmes AG, Chan MS, Mesa JL, et al. HSP72 protects against obesity-induced insulin resistance. PNAS 2008; 105(5): 1739-44.
27. Dimauro I, Antonioni A, Mercatelli N, Grazioli E, Fantini C, Barone R, et al. The early response of αB-crystallin to a single bout of aerobic exercise in mouse skeletal muscles depends upon fiber oxidative features. Redox Biol 2019; 24: 101183.
28. Morton JP, Maclaren D, Cable NT, Campbell IT, Evans L, Kayani AC, et al. Trained men display increased basal heat shock protein content of skeletal muscle. MSSE 2008; 40(7): 1255-62.
29. Ogata T, Oishi Y, Higashida K, Higuchi M, Muraoka I. Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. Am J Physiol Regul Integr Comp Physiol 2009; 296(5): R1557-R63.
30. Moura CS, Lollo PCB, Morato PN, Nisishima LH, Carneiro EM, Amaya-Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. Plos One 2014; 9(1): e83437.
31. Mattson JP, Ross CR, Kilgore J, Musch TI. Induction of mitochondrial stress proteins following treadmill running. MSSE 2000; 32(2): 365-9.
32. Talmadge RJ, Roy RR, Edgerton VR. Muscle fiber types and function. Curr Opin Rheumatol 1993; 5(6): 695-705.
33. Mattson JP, Ross CR, Kilgore JL, Musch TI, Induction of mitochondrial stress proteins following treadmill running. Med.Sci. Sports Exerc 2000, 32, 365–9.
34. Barone R, Macaluso F, Sangiorgi C, Campanella C, Marino Gammazza A, Moresi V, et al. Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 alpha1 expression. Sci Rep 2016; 6(1): 1-8.
35. Voos W, Röttgers K. Molecular chaperones as essential mediators of mitochondrial biogenesis. BBA 2002; 1592(1): 51-62.
36. Itariu BK, Stulnig TM. Autoimmune aspects of type 2 diabetes mellitus-a mini-review. Gerontology 2014 ;60(3): 189-96.
37. Cerf ME. Beta cell dysfunction and insulin resistance. Front. Endocrinol 2013; 4: 37.
38. Gandhi SM, Nylen ES, Sen S. The Role of Physical Activity on Insulin Resistance-Associated Endothelial Dysfunction. IntechOpen 2019.
39. Montgomery MK, Turner N. Mitochondrial dysfunction and insulin resistance: an update. Endocrine Connections 2015; 4(1): R1-R15.
40. CfDCaP C. National diabetes statistics report, 2017. Atlanta, GA: Centers for Disease Control and Prevention, US Department of Health and Human Services. 2017.
41. Olver TD, Laughlin MH, Padilla J. Exercise and vascular insulin sensitivity in skeletal muscle and brain. Exerc Sport Sci Rev 2019; 47(2): 66.
42. Nylén ES, Kokkinos P. Physical activity, cardiorespiratory fitness, and the diabetes spectrum. Cardiorespiratory Fitness in Cardiometabolic Diseases: Springer; 2019. p. 191-206.
43. Meex RC, Schrauwen-Hinderling VB, Moonen-Kornips E, Schaart G, Mensink M, Phielix E, et al. Restoration of muscle mitochondrial function and metabolic flexibility in type 2 diabetes by exercise training is paralleled by increased myocellular fat storage and improved insulin sensitivity. Diabetes 2010; 59(3): 572-9.
44. McConell GK, Ng GPY, Phillips M, Ruan Z, Macaulay SL, Wadley GD. Central role of nitric oxide synthase in AICAR and caffeine-induced mitochondrial biogenesis in L6 myocytes. ‌J Appl Physioly 2010; 108(3): 589-95.
45. Hey-Mogensen M, Højlund K, Vind B, Wang L, Dela F, Beck-Nielsen H, et al. Effect of physical training on mitochondrial respiration and reactive oxygen species release in skeletal muscle in patients with obesity and type 2 diabetes. Diabetologia 2010; 53(9): 1976-85.
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Shabani M, Valipour-Dehnou V, Tabandeh M R, Molanouri-Shamsi M. The effect of aerobic endurance exercise on changes in heat shock protein 60 and insulin resistance in mice with type 2 diabetes. Feyz 2022; 26 (3) :273-281
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Volume 26, Issue 3 (Bimonthly 2022) Back to browse issues page
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