:: Volume 26, Issue 4 (Bimonthly 2022) ::
Feyz 2022, 26(4): 407-415 Back to browse issues page
Effect of eight weeks of high-intensity intermittent training (HIIT) and low- intensity endurance training (LIET) with a high-fat diet on the expression of PPARα and PLIN5 of skeletal tissue in overweight male rats
Samira Sadeghi , Farzaneh Taghian
Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, I.R. Iran. , F_TAGHIAN@YAHOO.COM
Abstract:   (1111 Views)
Background: Obesity is a metabolic disorder in which proteins involved in fat metabolism are altered. Because obesity is a growing disease among people living in today's societies, the purpose of this study was to compare the effects of 8 weeks of high-intensity intermittent training and low- intensity endurance training on PPARα and PLIN5 gene expression in skeletal muscle of overweight rats.
Materials and Methods: Forty male Wistar rats, 5 weeks old, weighing 148.4 ±22.8 g were randomly divided into four equal groups (n=10): control, LIET, HIIT which fed high fat diet, and sham group which fed standard diet. Forty-eight hours after the end of the training program (eight weeks of intense intermittent exercise or HIIT and continued low-intensity exercise or (LIET) the rat adipose tissue was isolated under sterile conditions. Finally, expression levels of PPARα and PLIN5 of skeletal tissue were measured by the Real-time PCR method.
Results: The data indicated that the expression level of the PPARα and PLIN5 was significantly increased in HIIT and LIET groups compared to the control group (P= 0.001).
Conclusion: HIIT and LIET exercises increased the expression of the PLINS gene in skeletal muscle by increasing the expression of the PPARα gene in lipid droplets of skeletal muscle.
Keywords: High-intensity intermittent exercise, Low-intensity exercise, Peroxisome alpha proliferator active receptor, PLIN5
Full-Text [PDF 383 kb]   (1433 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/12/13 | Revised: 2022/09/28 | Accepted: 2022/07/30 | Published: 2022/09/26
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Volume 26, Issue 4 (Bimonthly 2022) Back to browse issues page