[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Articles archive::
For Reviewers::
Contact us::
Basic and Clinical Biochemistry and Nutrition
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 26, Issue 1 (Bimonthly 2022) ::
Feyz 2022, 26(1): 30-37 Back to browse issues page
The effect of eight weeks of high intensity interval training on Let-7a, NO and VEGF levels in cardiac tissue of young rats with type 2 diabetes
Maryam Bagherinia , Bahram Abedi , Hosein Fatolahi
Department of Exercise Physiology, Mahallat Branch, Islamic Azad University, Mahallat, I.R. Iran. , maryambagheri14@yahoo.com
Abstract:   (1121 Views)
Background: Diabetes interferes with some of the factors that contribute to cardiac angiogenesis. So, this study aimed to investigate the effect of eight weeks of high-intensity interval training (HIIT) on VEGF, NO and Let-7a in young rats with type 2 diabetes.
Materials and Methods: The present study is an experimental study in which the statistical population consisted of young rats (8 weeks) with type 2 diabetes. A total of 24 young rats (8 weeks old) were divided into three groups: diabetic + HIIT, healthy + HIIT + healthy control. ELISA was used to measure VEGF and NO and Real time PCR was used for Le7-a.
Results: Diabetes + HIIT group had more VEGF compared to healthy control group (P= 0.005) and healthy + HIIT group had more VEGF than healthy control group (P=0.001). The healthy + HIIT group also had higher VEGF than the diabetes + HIIT group (P=0.005). The diabetes + HIIT group had more NO than the healthy control group (P=0.041) and the healthy + HIIT group had more NO compared to the healthy control group (P=0.005). Also, the difference between the healthy + healthy group in comparison with the diabetes + healthy group was not significant in terms of NO (P=0.07). The diabetic + healthy group had more Let-7a than the healthy control group (P=0.009) and the healthy + HIIT group had more Let-7a than the healthy control group (P=0.001). Also, the healthy + HIIT group had a higher Let-7a value compared to the diabetes + HIIT group (P=0.001).
Conclusion: The findings of this study showed that HIIT increases VEGF, Let-7a and NO in diabetic and healthy rats. However, the increase in these variables was higher in healthy samples than in diabetic samples.
Keywords: Type 2 diabetes, High-intensity interval training, Angiogenesis
Full-Text [PDF 334 kb]   (520 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/07/24 | Revised: 2022/04/6 | Accepted: 2021/12/28 | Published: 2022/04/4
1. Carmeliet P, Luttun A. The emerging role of the bone marrow-derived stem cells in (therapeutic) angiogenesis. Thromb Haemost 2001;86:289-97.
2. van Royen N, Piek JJ, Schaper W, Bode C, Buschmann I. Arteriogenesis: mechanisms and modulation of collateral artery development. J Nucl Cardiol 2001;8:687-93.
3. Abacı A, Oguzhan A, Kahraman S, Eryol NK, Ünal Suk, Arınç Hs, et al. Effect of diabetes mellitus on formation of coronary collateral vessels. Circulation 1999; 99: 2239-42.
4. Werner GS, Ferrari M, Betge S, Gastmann O, Richartz BM, Figulla HR. Collateral function in chronic total coronary occlusions is related to regional myocardial function and duration of occlusion. Circulation 2001;104:2784-90.
5. Hegde P, Wallin J, Mancoa Ch. Predictive markers of anti-VEGF and emerging role of angiogenesis inhibitors as immunotherapeutics. Semin Cancer Biol 2018; 52(Pt 2): 117-24.‌
6. Green DJ, Spence A, Halliwill JR, Cable NT, Thijssen DH. Exercise and vascular adaptation in asymptomatic humans. Exp Physiol 2011; 96: 57-70.
7. Yoon YS, Uchida S, Masuo O, Cejna M, Park J-S, Gwon HC, et al. Progressive attenuation of myocardial vascular endothelial growth factor expression is a seminal event in diabetic cardiomyopathy: restoration of microvascular homeostasis and recovery of cardiac function in diabetic cardiomyopathy after replenishment of local vascular endothelial growth factor. Circulation 2005; 11: 12073-85.
8. Mahrou M, Gaeini AA, Chobbineh S, Javidi M. Changes in stimulating factors of angiogenesis, induced by progressive resistance training in diabetic rats. Ijdld 2014; 14: 1-8.
9. Miao Y, Ajami NE, Huang TS, Lin FM, Lou CH, Wang YT, et al. Enhancer-associated long non-coding RNA LEENE regulates endothelial nitric oxide synthase and endothelial function. Nat Commun 2018; 9(1): 1-3.
10. Sokolovsk A, Dekante A, Baumane L, Pahirko L, Valeinis J, Dislere K, et al. Nitric oxide metabolism is impaired by type 1 diabetes and diabetic nephropathy. Biomed Rep 2020; 12.5: 251-258.‌
11. Jahangiri RJ, Farzanegi P, Habibian MH. The Effect of Aerobic Training and Arbotin on Cardiac Nitric Oxide, Tumor Necrosis Factor alpha, and Vascular Endothelial Growth Factor in Male Diabetic Rats. Qom Univ Med Sci J 2017;11:53-62. [in Persian]
12. Küçükali CI, Kürtüncü M, Coban A, Cebi M, Tüzün E. Epigenetics of multiple sclerosis: an updated review. Neuromolecular Med 2015;17:83-96.
13. Jiang S. Recent findings regarding let-7 in immunity. Cancer Lett 2018, 434: 130-1.‌
14. Berindan-Neagoe I, Calin GA. Molecular pathways: microRNAs, cancer cells, and microenvironment. Clin Cancer Res 2014;20:6247-53.
15. Kuehbacher A, Urbich C, Zeiher AM, Dimmeler S. Role of Dicer and Drosha for endothelial microRNA expression and angiogenesis. Circ Res 2007; 101: 59-68.
16. Chen Z, Lai T-C, Jan YH, Lin FM, Wang WC, Xiao H, et al. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. J Clin Invest 2013; 123: 1057-67.
17. Satoh M, Tabuchi T, Minami Y, Takahashi Y, Itoh T, Nakamura M. Expression of let-7i is associated with Toll-like receptor 4 signal in coronary artery disease: effect of statins on let-7i and Toll-like receptor 4 signal. Immunobiology 2012; 217: 533-9.
18. Santovito D, De Nardis V, Marcantonio P, Mandolini C, Paganelli C, Vitale E, et al. Plasma exosome microRNA profiling unravels a new potential modulator of adiponectin pathway in diabetes: effect of glycemic control. J Clin Endocrinol Metab 2014; 99: E1681-E5.
19. Sigal RJ, Kenny GP, Wasserman DH, Castaneda-Sceppa C. Physical activity/exercise and type 2 diabetes. Diabetes care 2004;27:2518-39.
20. Vizvari E, Farzanegi P, Abaszade Sourati H. Effect of Moderate Aerobic Training on Serum Levels of Angiogenic and Angiostatic Factors in Women with Type 2 Diabetes. Sjimu 2019; 27: 112-21. [in Persian]
21. Isanejad A, Alizadeh AM, Shalamzari SA, Khodayari H, Khodayari S, Khori V, et al. MicroRNA-206, let-7a and microRNA-21 pathways involved in the anti-angiogenesis effects of the interval exercise training and hormone therapy in breast cancer. Life Sci 2016; 151: 30-40.
22. Akbarzadeh A, Fattahi bafghi A. The effect of high intencity interval training combined with curcumin supplementation on Plasma glucose concentration and insulin resistance in diabetic rats. Jssu 2018;25:961-9. [in Persian]
23. Brennan E, Wang B, McClelland A, Mohan M, Marai M, Beuscart O, et al. Protective effect of let-7 miRNA family in regulating inflammation in diabetes-associated atherosclerosis. Diabetes 2017;66:2266-77.
24. Bernsten D L, Jiang, X, Rom S. let-7 microRNAs: Their Role in Cerebral and Cardiovascular Diseases, Inflammation, Cancer, and Their Regulation. Biomedicines 2021, 9.6: 606.‌
25. Otsuka M, Zheng M, Hayashi M, Lee J-D, Yoshino O, Lin S, et al. Impaired microRNA processing causes corpus luteum insufficiency and infertility in mice. J Clin Invest 2008;118:1944-54.
26. Bae ON, Wang JM, Baek SH, Wang Q, Yuan H, Chen AF. Oxidative Stress–Mediated Thrombospondin-2 Upregulation Impairs Bone Marrow–Derived Angiogenic Cell Function in Diabetes Mellitus. Arterioscler Thromb Vasc Biol 2013; 33: 1920-7.
27. Park JT, Kato M, Lanting L, Castro N, Nam BY, Wang M, et al. Repression of let-7 by transforming growth factor-β1-induced Lin28 upregulates collagen expression in glomerular mesangial cells under diabetic conditions. Am J Physiol Renal Physiol 2014; 307: F1390-F403.
28. Ko JR, Seo DY, Kim TN, Park SH, Kwak H-B, Ko KS, et al. Aerobic Exercise Training Decreases Hepatic Asprosin in Diabetic Rats. J Clin Med 2019; 8: 666.
29. Cerychova R, Pavlinkova G. HIF-1, metabolism, and diabetes in the embryonic and adult heart. Front Endocrinol 2018; 9: 460.
30. Wang S, Tang Y, Zhang Z, Tang Z, Liu Y, Wang Z. Regulatory effects of HIF-1alpha on eNOS expressions in the vascular endothelium of prediabetic rats with aerobic exercise. Biomedical Res 2018.
31. Ren L, Sen U, Pushpakumar S. Exercise training reduces TGF‐β mediated epithelial mesenchymal transition in diabetic kidney. FASEB J 2017; 31: 1086.5-.5.
32. Nourshahi M, Ranjbar K. The stimulus of angiogenesis during exercise and physical activity. QHMS 2013; 286-96. [in Persian]
33. Soori R, CHoobine S. Effect of endurance training on VEGF protein level in tissue of cardiac muscle in STZ-induced diabetic Wistar rats. Yafteh 2018; 20: 110-24. [in Persian]
34. Ghorbanzadeh V, Mohammadi M, Dariushnejad H, Abhari A, Chodari L, Mohaddes G. Cardioprotective effect of crocin combined with voluntary exercise in rat: role of mir-126 and mir-210 in heart angiogenesis. Arq Bras Cardiol 2017; 109: 54-62.
35. Shoeibi S, Mozdziak P, Mohamadi S. Important signals regulating coronary artery angiogenesis. Microvasc Res 2018; 117: 1-9.‌
36. Salehi E, Amjadi FS, Khazaei M. Angiogenesis in Health and Disease: Role of Vascular Endothelial Growth Factor (VEGF). JIMS 2011; 29. [in Persian]
37. Grijalva J, Hicks S, Zhao X, Medikayala S, Kaminski PM, Wolin MS, et al. Exercise training enhanced myocardial endothelial nitric oxide synthase (eNOS) function in diabetic Goto-Kakizaki (GK) rats. Cardio Diabet 2008; 7: 34.
38. Krause M, Josianne RK, Ciara O, Paul M, Gareth D, Davide S, et al. The effects of aerobic exercise training at two different intensities in obesity and type 2 diabetes: implications for oxidative stress, low-grade inflammation and nitric oxide production. Eur J Appl Physiol 2014; 114.2: 251-260.‌
39. Bao M-h, Zhang Y-w, Lou X-y, Cheng Y, Zhou H-h. Protective effects of let-7a and let-7b on oxidized low-density lipoprotein induced endothelial cell injuries. PLoS One 2014; 9: e106540.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bagherinia M, Abedi B, Fatolahi H. The effect of eight weeks of high intensity interval training on Let-7a, NO and VEGF levels in cardiac tissue of young rats with type 2 diabetes. Feyz 2022; 26 (1) :30-37
URL: http://feyz.kaums.ac.ir/article-1-4436-en.html

Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 26, Issue 1 (Bimonthly 2022) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4642