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:: Volume 26, Issue 1 (Bimonthly 2022) ::
Feyz 2022, 26(1): 38-46 Back to browse issues page
The effect of aerobic exercise with ozone and stem cell on anandamide concentration in desert rat with osteoarthritis
Maryam Rezaei , Mohamadali Azarbayjani , Maghsoud Piree , Seid Ali Hosseini
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, I.R. Iran. , ali.azarbayjani@gmail.com
Abstract:   (1265 Views)
Background: Aerobic exercise, ozone therapy and cell therapy have each been independently proposed as treatments for osteoarthritis. Therefore, the present study aimed to investigate the effect of aerobic exercise with ozone and cell therapy on serum levels of anandamide concentration in osteoarthritis rats.
Materials and Methods: Forty-five male rats were randomly divided into 9 groups, including 1 group: healthy control, 2-patient control, 3-exercise, 4-ozone therapy, 5-cell therapy, 6-exercise+ozone therapy, 7-exercise+cell therapy, 8-ozone+cell therapy and 9-ozone+cell therapy+exercise. Cell groups alone and combination cell therapy received 1×106cells/kg, and ozone groups alone and ozone combination therapies received 20µg/ml of ozone in 3 replications before exercise. The training program consisted of running on a treadmill at a speed of 16 meters per minute for 8 weeks. Forty-eight hours after the last exercise session, serum levels of anandamide were measured by Elisa.
Results: Findings showed that the serum level of anandamide in the osteoarthritis patient group was significantly reduced compared to the healthy group that exercise and ozone therapy led to a significant increase in the serum anandamide level; but cell therapy could not have a significant effect on increasing serum levels of anandamide. The peak of this increase was seen in ozone+cell therapy and the interaction of all three interventions simultaneously.
Conclusion: Although aerobic exercise and ozone therapy each independently have a positive effect on anandamide in people with osteoarthritis, but the interaction of all three interventions simultaneously with ozone+cell therapy is more effective.
Keywords: Aerobic exercise, Stem cell, Ozone therapy, Osteoarthritis, Anandamide
Full-Text [PDF 372 kb]   (527 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/06/28 | Revised: 2022/04/17 | Accepted: 2022/01/31 | Published: 2022/04/4
References
1. Jalilian J, Behpoor N, Farzanegi P. Effect of Aerobic Training in Combination with Stem Cells on Inflammatory Biomarker Levels in the Heart Tissue of Rat Model of Osteoarthritis. J Ilam Univ Med Sci 2020; 28(1): 12-26. [in Persian]
2. Bryk M, Chwastek J, Kostrzewa M, Mlost J, Pędracka A, Starowicz K. Alterations in anandamide synthesis and degradation during osteoarthritis progression in an animal model. Int J Mol Sci 2020; 21(19): 7381.
3. Galdino G, Romero T, da Silva JF, Aguiar D, de Paula AM, Cruz J, et al. Acute resistance exercise induces antinociception by activation of the endocannabinoid system in rats. Anesth Analg 2014; 119(3): 702.
4. Sparling P, Giuffrida A, Piomelli D, Rosskopf L, Dietrich A. Exercise activates the endocannabinoid system. Neuro Report 2003; 14(17): 2209-11.
5. Petrosino S, Palazzo E, de Novellis V, Bisogno T, Rossi F, Maione S, et al. Changes in spinal and supraspinal endocannabinoid levels in neuropathic rats. Neuropharmacology 2007; 52(2): 415-22.
6. Starowicz K, Makuch W, Korostynski M, Malek N, Slezak M, Zychowska M, et al. Full inhibition of spinal FAAH leads to TRPV1-mediated analgesic effects in neuropathic rats and possible lipoxygenase-mediated remodeling of anandamide metabolism. PloS One 2013; 8(4): e60040.
7. Valastro C, Campanile D, Marinaro M, Franchini D, Piscitelli F, Verde R, et al. Characterization of endocannabinoids and related acylethanolamides in the synovial fluid of dogs with osteoarthritis: A pilot study. BMC Vet Res 2017; 13(1): 1-5.
8. Richardson D, Pearson RG, Kurian N, Latif ML, Garle MJ, Barrett DA, et al. Characterisation of the cannabinoid receptor system in synovial tissue and fluid in patients with osteoarthritis and rheumatoid arthritis. Arthritis Res Ther 2008; 10(2): 1-4.
9. Watkins BA. Endocannabinoids, exercise, pain, and a path to health with aging. Mol Aspects Med 2018; 64: 68-78.
10. Chahal J, Gómez-Aristizábal A, Shestopaloff K, Bhatt S, Chaboureau A, Fazio A, et al. Bone marrow mesenchymal stromal cell treatment in patients with osteoarthritis results in overall improvement in pain and symptoms and reduces synovial inflammation. Stem Cells Transl Med 2019; 8(8): 746-57.
11. Mancuso P, Raman S, Glynn A, Barry F, Murphy JM. Mesenchymal stem cell therapy for osteoarthritis: the critical role of the cell secretome. Front Bioeng Biotechnol 2019; 9.
12. Ham O, Lee CY, Kim R, Lee J, Oh S, Lee MY, et al. Therapeutic potential of differentiated mesenchymal stem cells for treatment of osteoarthritis. Int J Mol Sci 2015; 16(7): 14961-78.
13. Asadi S, Farzanegi P, Azarbayjani MA. Effect of exercise, ozone and mesenchymal stem cells therapies on expression of IL-10 and TNF-α in the cartilage tissue of overweight rats with knee osteoarthritis. Soc Determ Heal 2018; 4(3): 162-70. [in Persian]
14. Rezaie M, Azarbayjani MA, Peeri M, Hosseini SA. The effect of exercise, Ozone, and Mesenchymal stem cells therapy on CB-1 and GABA Gene expression in the cartilage tissue of rats with knee osteoarthritis. Pharm Biomed Res 2020; 6(1): 45-52. [in Persian]
15. Kaya A, Sonmez M, Kar T, Haholu A, Yildirim Y, Müftüoğlu T, et al. Efficiency of ozone therapy in a rat model of experimental uveitis. Ocul Immunol Inflamm 2017; 25(5): 700-5.
16. Jalilian J, Behpour N, Hosseinpour Delavar S, Farzaneghi P. A Study of the Effect of the Aerobic Training Together with Stem Cells on the Levels of Some of the Apoptosis Indices of the Heart Tissue of the Male Wistar Rats of Osteoarthritis Model. Jundishapur J Heal Sci 2019; 18(6): 585–602. [in Persian]
17. Kapuscinski J. DAPI: a DNA-specific fluorescent probe. Biotechnic Histochemistry 1995; 70(5): 220-33.
18. Li M, Luo X, Lv X, Liu V, Zhao G, Zhang X, et al. In vivo human adipose-derived mesenchymal stem cell tracking after intra-articular delivery in a rat osteoarthritis model. Stem Cell Res Ther 2016; 7(1): 1-3.
19. Lopes de Jesus CC, Dos Santos FC, de Jesus LM, Monteiro I, Sant’Ana MS, Trevisani VF. Comparison between intra-articular ozone and placebo in the treatment of knee osteoarthritis: A randomized, double-blinded, placebo-controlled study. PloS One 2017; 12(7): e0179185.
20. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Preventive Cardiol 2007; 14(6): 753-60.
21. Liu SS, Zhou PU, Zhang Y. Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis. Mol Med Report 2016; 13(3): 1999-2006.
22. Stensson N, Gerdle B, Ernberg M, Mannerkorpi K, Kosek E, Ghafouri B. Increased anandamide and decreased pain and depression after exercise in fibromyalgia. Med Sci Sports Exerc 2020; 52: 1617-28.
23. Lima LV, Abner TS, Sluka KA. Does exercise increase or decrease pain? Central mechanisms underlying these two phenomena. J Physiol 2017; 595(13):4141-50.
24. Cuadros ME, Moro OS, Florin MJ, Canelo JA. Ozone improves pain, function and quality of life in patients with knee osteoarthritis: A prospective quasi-experimental before-after study. Middle East J Rehabil Heal 2017; 4(1).
25. Freitag J, Bates D, Boyd R, Shah K, Barnard A, Huguenin L, et al. Mesenchymal stem cell therapy in the treatment of osteoarthritis: reparative pathways, safety and efficacy–a review. BMC Musculoskelet Disord 2016; 17(1): 1-3.
26. Abdizadeh L, Peeri M, Azarbayjani MA, Farzanegi P. The effect of aerobic exercise, mesenchymal stem cells (MSCs) and ozone therapy on anxiety and like depression behaviors caused by induction of osteoarthritis in rats. Razi J Med Sci 2021; 28(1): 12-22. [in Persian]
27. Gilani A, Gaeini A, Nouri R. Effect of aerobic exercise training and ozone therapy on TRF2 and TERT gene expression in heart tissue of rats with osteoarthritis. Stud Med Sci 2020; 31(3): 169-77. [in Persian]
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Rezaei M, Azarbayjani M, Piree M, Hosseini S A. The effect of aerobic exercise with ozone and stem cell on anandamide concentration in desert rat with osteoarthritis. Feyz 2022; 26 (1) :38-46
URL: http://feyz.kaums.ac.ir/article-1-4424-en.html


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Volume 26, Issue 1 (Bimonthly 2022) Back to browse issues page
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