:: Volume 25, Issue 6 (Bimonthly 2021) ::
Feyz 2021, 25(6): 1286-1293 Back to browse issues page
The effect of aerobic training and stevia on the expression of miR-322 and cyclin D1 in heart tissue of type 1 diabetic rats
Kobra Soleymani , Asieh Abbassi-Daloii , Seyed Javad Ziaolhagh , Ayoub Saeidi
Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, I.R. Iran. , abbasi.daloii@gmail.com
Abstract:   (889 Views)
Background: A close link exists between diabetes and cardiovascular disease. cardiovascular disease is the most prevalent cause of mortality and morbidity in diabetic populations. The response of biomarkers such as miRNA to environmental factors is important for optimizing diabetes treatment strategies. This study aimed to evaluate the effect of aerobic training and stevia supplement on the expression of miR-322 and cyclin D1 in heart tissue of type 1 diabetic rats.
Materials and Methods: In this experimental study, 25 type 1 diabetic rats (weight 300-250 g) were divided into 5 groups; healthy control, type 1 diabetic control, type 1 diabetic – stevia supplement, type 1 diabetic-exercise and type 1 diabetic - exercise - stevia supplement. Aerobic training was performed 5 days a week at a speed of 20 to 30 meters per minute and a slope of zero degrees for 8 weeks. The dose of stevia was 250 mg per kilogram of body weight in gavage. Rats were slaughtered 48 hours after the last training session and Cardiac tissue was used to analyze the indicators. The gene expression of miR-322 and cyclin D1 in cardiac tissue was measured by Real Time PCR. Data were analyzed by One-way ANOVA and Bonferron's post hoc test at the P<0.05.
Results: Aerobic training, stevia and the interaction of aerobic training and stevia supplementation had no significant effect on the gene expression of miR-322 and cyclin D1 in the heart tissue of type-1 diabetic rats (P<0.05).
Conclusion: According to the results of the study, it seems that intervention of aerobic training with stevia supplementation has no effect on the gene expression of cardiac metabolic markers in type 1 diabetes.
Keywords: Exercise, Stevia, miR-322, Cyclin D1, Type 1 diabetes
Full-Text [PDF 392 kb]   (562 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/02/21 | Revised: 2023/11/1 | Accepted: 2021/12/6 | Published: 2022/02/1
References
1. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010; 87(1): 4-14.
2. Vaidya V, Gangan N, Sheehan J. Impact of cardiovascular complications among patients with Type 2 diabetes mellitus: a systematic review. Expert Rev Pharmacoecon Outcomes Res 2015; 15(3): 487–97.
3. Ghosh N, Katare R. Molecular mechanism of diabetic cardiomyopathy and modulation of microRNA function by synthetic oligonucleotides. Cardiovasc Diabetol 2018; 17: 43.
4. Bradley TJ, Slorach C, Mahmud FH, Dunger DB, Deanfield J, Deda L, et al. Early changes in cardiovascular structure and function in adolescents with type 1 diabetes. Cardiovasc Diabetol 2016; 15(1): 31.
5. From AM, Leibson CL, Bursi F, Redfield MM, Weston SA, Jacobsen SJ, et al. Diabetes in heart failure: prevalence and impact on outcome in the population. Am J Med 2006; 119(7): 591–9.
6. Karolina DS, Arunmozhiarasi A, Sugunavathi S, Kandiah J. miRNAs and diabetes mellitus. Expert Review Endocrinol Metabol 2012; 7)3:( 281-300.
7. Krol J, Loedige I, Filipowicz W. The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet 2010; 11: 597–610.
8. Gámez B, Rodríguez-Carballo E, Bartrons R, Rosa JL, Ventura F. MicroRNA-322 (miR-322) and its target protein Tob2 modulate Osterix (Osx) mRNA stability. J Biological Chemistry 2013; 288(20): 14264-75.
9. Sarkar S, Dey BK, Dutta A. MiR-322/424 and-503 are induced during muscle differentiation and promote cell cycle quiescence and differentiation by down-regulationof Cdc25A. Molecular Biology Cell 2010; 21(13): 2138-49.
10. Merlet E, Atassi F, Motiani RK, Mougenot N, Jacquet A, Nadaud S, et al. miR-424/322 regulates vascular smooth muscle cell phenotype and neointimal formation in the rat. Cardiovascular Res 2013; 98(3): 458-68.
11. Bhalla K, Liu WJ, Thompson K, Anders L, Devarakonda S, Dewi R, et al. Cyclin D1 represses gluconeogenesis via inhibition of the transcriptional coactivator PGC1α. Diabetes 2014; 63(10): 3266-78
12. Marchand A, Atassi F, Mougenot N, Clergue M, Codoni V, Berthuin J, et al. miR-322 regulates insulin signaling pathway and protects against metabolic syndrome-induced cardiac dysfunction in mice. Basis Dis 2016; 1862(4): 611-21.
13. Nesca V, Guay C, Jacovetti C, Menoud V, Peyot ML, Laybutt DR, et al. Identification of particular groups of microRNAs that positively or negatively impact on beta cell function in obese models of type 2 diabetes. Diabetologia 2013; 56(10): 2203-12.
14. Lang H, Ai Z, You Z, Wan Y, Guo W, Xiao J, et al. Characterization of miR-218/322-Stxbp1 pathway in the process of insulin secretion. J Mol Endocrinol 2015; 54(1): 65-73.
15. Nascimento, M.S., Espindola, C.F., do Prado, C. et al. Type 1 diabetes does not impair the physical capacity of non-sedentary adolescents. Diabetol Metab Syndr 2017; 9: 100-8
16. Wilson LC, Peebles KC, Hoye NA, Manning P, Sheat C, Williams MJA, et al. Resting heart rate variability and exercise capacity in Type 1 diabetes. Physiol Rep 2017; 5(8): e13248.
17. Roncarati R, Viviani Anselmi C, Losi MA, Papa L, Cavarretta E, Da Costa Martins P, et al. Circulating miR-29a, among other up-regulated microRNAs, is the only biomarker for both hypertrophy and fibrosis in patients with hypertrophic cardiomyopathy. J Am Coll Cardiol 2014; 63: 920–7
18. Goyal SK, Samsher Goyal RK. Stevia (Stevia rebaudiana) a bio-sweetener: a review. Int J Food Sci Nutr 2010; 61: 1–10.
19. Ajami M, Seyfi M, Abdollah Pouri Hosseini F, Naseri P, Velayati A, Mahmoudnia F, et al. Effects of stevia on glycemic and lipid profile of type 2 diabetic patients: A randomized controlled trial. Avicenna J Phytomed 2020; 10(2): 118-27.
20. Boonkaewwan C, Burodom A. Anti-inflammatory and immunomodulatory activities of stevioside and steviol on colonic epithelial cells. J Sci Food Agric 2013; 93(15): 3820-5.
21. Masoumi S, Ranjbar S, Keshavarz V. The Effectiveness of Stevia in Diabetes Mellitus: A Review. International J Nutrition Sci 2020; 5(2): 45-9.
22. Gregersen S, Jeppesen PB, Holst JJ, Hermansen K. Antihyperglycemic effects of stevioside in type 2 diabetic subjects. Metabolism 2004; 53(1): 73-6.
23. Ahmad U, Ahmad RS. Anti-diabetic property of aqueous extract of Stevia rebaudiana Bertoni leaves in Streptozotocin-induced diabetes in albino rats. BMC Complementary Alternative Med 2018; 18(1): 179.
24. Jang J, Jung Y, Seo SJ, Kim SM, Shim YJ, Cho SH, et al. Berberine activates AMPK to suppress proteolytic processing, nuclear translocation and target DNA binding of SREBP-1c in 3T3-L1 adipocytes. Mol Med Rep 2017; 15(6): 4139-47.
25. Marchand A, Atassi F, Mougenot N, Clergue M, Codoni V, Berthuin J, et al. miR-322 regulates insulin signaling pathway and protects against metabolic syndrome-induced cardiac dysfunction in mice. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 2016; 1862(4): 611-21
26. Merlet E, Atassi F, Motiani RK, Mougenot N, Jacquet A, Nadaud S, et al. miR-424/322 regulates vascular smooth muscle cell phenotype and neointimal formation in the rat. Cardiovascular Res 2013; 98(3): 458-68
27. Figueiredo VC, et al. Ribosome biogenesis adaptation in resistance training-induced human skeletal muscle hypertrophy. Am J Physiol Endocrinol Metab 2015; 309: 72–83.
28. Kadi F, Schjerling P, Andersen LL, Charifi N, Madsen JL, Christensen LR, et al. The effects of heavy resistance training and detraining on satellite cells in human skeletal muscles. J Physiol 2004; 558(3): 1005-12.
29. Lee Y, Dominy JE, Choi YJ, Jurczak M, Tolliday N, Camporez JP, et al. Cyclin D1–Cdk4 controls glucose metabolism independently of cell cycle progression. Nature 2014; 510: 547–51.
30. Hinds PW, Brown NE. D-Type Cyclins and Cancer. Current Cancer Res 2018; 61–90.
31. Mathupala SP, Rempel A, Pedersen PL. Glucose catabolism in cancer cells: identification and characterization of a marked activation response of the type II hexokinase gene to hypoxic conditions. J Biol Chem 2011; 276: 43407–12.
32. Liu X, Platt C, and Rosenzweig A. The Role of MicroRNAs in the Cardiac Response to Exercise Cold Spring. Harb Perspect Med 2017; 7(12): a029850.
33. Soci UP, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, et al. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiol Genomics 2011; 43(11): 665–73.
34. Kujur RS, Singh V, Ram M, Yadava HN, Singh KK, et al. Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats. Pharmacognosy Res 2010; 2: 258-63.
35. Ray J, Kumar S, Laor D, Shereen N, Nwamaghinna F, Thomson A, et al. Effects of Stevia Rebaudiana on Glucose Homeostasis, Blood Pressure and Inflammation: A Critical Review of Past and Current Research Evidence. Int J Clin Res Trials 2020; 5: 142.
36. Shukla S, Mehta A, Bajpai VK. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chemical Toxicol 2009; 47: 2338-43.
37. Gupta E, Purwar SH, Sundaram SH, Rai G. Nutritional and therapeutic values of Stevia rebaudiana: A review. J Med Plants Res 2013; 7: 3343-53.

XML   Persian Abstract   Print

Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 25, Issue 6 (Bimonthly 2021) Back to browse issues page