:: Volume 25, Issue 5 (Bimonthly 2021) ::
Feyz 2021, 25(5): 1155-1167 Back to browse issues page
Protective effect of ascorbic acid on sperm parameters and testis structure of the first-generation mouse following mancozeb exposure
Esmaeil Saddein , Tahereh Haghpanah , Seyed Noreddin Nematollahi-Mahani , Massood Ezzatabadipour , Tania Dehesh
Kerman University of Medical Sciences , ezzatabadipm@gmail.com
Abstract:   (994 Views)
Background: This study aimed to investigate the possible protective effects of ascorbic acid on mancozeb-induced toxicity on sperm parameters and testicular structure in the first-generation mice pups.
Materials and methods: In this experimental study, pregnant female NMRI mice were randomly divided into four groups including: control, sham, mancozeb (500 mg/ kg) and ascorbic acid+ mancozeb (100 mg/ kg ascorbic acid + 500 mg/ kg mancozeb). Treatments started at the first day of pregnancy and continued until weaning. Male pups from each group were maintain until puberty (8-10 weeks). Left testicular weight was measured and its structure was studied. Then, the left epididymis was isolated, and sperm parameters (number, viability, motility and morphology) were studied.
Results: A significant decrease was observed in the sperm parameters of mancozeb group compared to the control and sham groups. However, concomitant use of ascorbic acid could improve the mancozeb-induced negative effects. Also, testicular parameters including weight, gonadosomatic index, diameter of seminiferous tubules, Johnsen`s score, as well as the number of germ cells including spermatogonia, spermatocytes, spermatids and Sertoli showed a significant decrease compared to the control and sham groups, also. While concomitant use of ascorbic acid could improve most of these damaged testicular and sperm parameters.
Conclusion: This study was showed that consumption of ascorbic acid along during pregnancy and breastfeeding periods has a protective effect against the destructive effects of mancozeb on sperm parameters and testicular structure of first-generation male pups.
Keywords: Mancozeb, Ascorbic acid, Pregnancy, Breastfeeding period, Testis, Spermatozoa
Full-Text [PDF 1233 kb]   (595 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2021/01/9 | Revised: 2021/12/25 | Accepted: 2021/09/12 | Published: 2021/11/28



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Volume 25, Issue 5 (Bimonthly 2021) Back to browse issues page