:: Volume 23, Issue 4 (Bimonthly 2019) ::
Feyz 2019, 23(4): 407-414 Back to browse issues page
Effect of an incremental aerobic training program on improving angiogenesis and balance in vascular endothelial growth factor and endostatinic in male rats with myocardial infarction
Mohammad MalekiPoya , Bahra Abedi , Mohammad Reza Palizvan , Abbas Saremi
Department of Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, I. R. Iran. , abedi@iaumahallat.ac.ir
Abstract:   (2668 Views)
Background: Vascular Endothelial Growth Factor (VEGF) and Endostatin (ES) are the most important causes of angiogenesis and improve cardiac function after myocardial infarction. One of the treatment methods for this disease is applying exercise program. Therefore, the aim of this study was to investigate the effect of a period of incremental aerobic training on improving the status of angiogenesis and balance in VEGF and ES factors in myocardial infarction in the rats.
Materials and Methods: In this experimental study with control group, 20 Wistar rats (8 weeks and weighing 230±30 g) were randomly divided into two groups (each group has 10 Rat) of incremental endurance training and the control group after induction of infarction with Isoproteronol (150 mg.kg). The training group was exposed to an aerobic training program for (8 weeks, 3 sessions per week, for 20 to 50 minutes at speeds of 12 to 18 meters per minute). Twenty four hours after the last training session, venous blood samples were collected from the serum levels of VEGF and ES and evaluated by ELISA method. Independent t-test was used at the significant level of P<0.05 to analyze the data.
Results: The results of t-test indicated that incremental aerobic training significantly increased serum levels of VEGF in rats with myocardial infarction (P=0.04). However, there was no significant change in serum ES levels after exercise program (P=0.58).
Conclusion: Incremental aerobic training seems to increase VEGF in the rats with myocardial infarction which can play an important role in the improvement of angiogenesis processes and the replacement of closed capillaries.
Keywords: Endostatin, Incremental aerobic training, Myocardial infarction, Vascular endothelial growth factor
Full-Text [PDF 334 kb]   (856 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/02/1 | Revised: 2019/09/30 | Accepted: 2019/06/19 | Published: 2019/09/30
1. Pagidipati NJ, Gaziano TA. Estimating deaths from cardiovascular disease: a review of global methodologies of mortality measurement. Circulation 2013; 127(6): 749-56.
2. Estruch R, Ros E, Salas-Salvadó J, Covas M-I, Corella D, Arós F, et al. Primary prevention of cardiovascular disease with a Mediterranean diet. NEJM 2013; 368(14): 1279-90.
3. Leosco D, Rengo G, Iaccarino G, Golino L, Marchese M, Fortunato F, et al. Exercise promotes angiogenesis and improves β-adrenergic receptor signalling in the post-ischaemic failing rat heart. CVR 2007; 78(2): 385-94.
4. Sugiyama A, Hirano Y, Okada M, Yamawaki H. Endostatin Stimulates Proliferation and Migration of Myofibroblasts Isolated from Myocardial Infarction Model Rats. IJMS 2018; 19(3): 741.
5. Prapulla DV, Sujatha PB, Pradeep A. Gingival crevicular fluid VEGF levels in periodontal health and disease. J Periodontol 2007; 78 (9):1783-7.
6. Li L, Zeng H, Chen JX. Apelin-13 increases myocardial progenitor cells and improves repair postmyocardial infarction. AJP-Heart Circ 2012; 303(5): 605-18.
7. Terjung R. What makes vessels grow with exercise training. J Appl Physiol 2004; 97(3): 1119-28.
8. Carmeliet P. Angiogenesis in life, disease and medicine. Nature 2005; 438(7070): 932.
9. Thébaud B. Angiogenesis in lung development, injury and repair: implications for chronic lung disease of prematurity. Neonatology 2007; 91(4): 291-7.
10. Kasahara Y, Tuder RM, Cool CD, Lynch DA, Flores SC, Voelkel NF. Endothelial cell death and decreased expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 2 in emphysema. AJRCCM 2001; 163(3): 737-44.
11. Falk E, Shah PK, Fuster V. Coronary plaque disruption. Circulation 1995; 92(3): 657-71.
12. Erekat NS, Al-Jarrah MD, Al Khatib AJ. Treadmill exercise training improves vascular endothelial growth factor expression in the cardiac muscle of type I diabetic rats. Cardiol Res 2014; 5(1): 23.
13. Ranjbar K, Nazem F, Nazari A, Gholami M, Nezami AR, Ardakanizade M, et al. Synergistic effects of nitric oxide and exercise on revascularisation in the infarcted ventricle in a murine model of myocardial infarction. Excli J 2015; 14: 1104.
14. Gu JW, Shparago M, Tan W, Bailey AP. Tissue endostatin correlates inversely with capillary network in rat heart and skeletal muscles. Angiogenesis 2006; 9(2): 93-9.
15. Nourshahi M, Hedayati M, Nemati J, Ranjbar K, Gholamali M. Effect of 8 weeks endurance training on serum vascular endothelial growth factor and endostatin in wistar rats. Koomesh 2012; 13(4): 474-9.
16. Lloyd PG, Prior BM, Yang HT, Terjung RL. Angiogenic growth factor expression in rat skeletal muscle in response to exercise training. AJP-Heart Circ 2003; 284(5): 1668-78.
17. Bertinchant J, Robert E, Polge A, Marty-Double C, Fabbro-Peray P, Poirey S, et al. Comparison of the diagnostic value of cardiac troponin I and T determinations for detecting early myocardial damage and the relationship with histological findings after isoprenaline-induced cardiac injury in rats. Clin Chim Acta 2000; 298(1-2): 13-28.
18. Lobo Filho HG, Ferreira NL, Sousa RBd, Carvalho ERd, Lobo PLD, Lobo Filho JG. Experimental model of myocardial infarction induced by isoproterenol in rats. BJCVS 2011; 26(3): 469-76.
19. Afzalpour ME, Yousefi MR, Eivari SHA, Ilbeigi S. Changes in blood insulin resistance, GLUT4 & AMPK after continuous and interval aerobic training in normal and diabetic rats. J App Pharm Sci 2016; 6: 76-81.
20. Rivilis I, Milkiewicz M, Boyd P, Goldstein J, Brown MD, Egginton S, et al. Differential involvement of MMP-2 and VEGF during muscle stretch-versus shear stress-induced angiogenesis. AJP-Heart Circ 2002; 283(4): 430-8.
21. Hirota K, Semenza GL. Regulation of angiogenesis by hypoxia-inducible factor 1. CROH 2006; 59)1(: 15-26.
22. Mehrialvar Y, Ramazani A, Gaeini A, Golab F, Gheiratmand R. The effect of exercise intervention on angiogenesis gene expression )inducing and inhibiting factors( following myocardial ischemia-reperfusion. EBNESINA- J Med 2017; 19(3): 13-23.
23. Jones WS, Duscha BD, Robbins JL, Duggan NN, Regensteiner JG, Kraus WE, et al. Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training. Vasc Med 2012; 17(2): 94-100.
24. Duggan C, Xiao L, Wang CY, McTiernan A. Effect of a 12-month exercise intervention on serum biomarkers of angiogenesis in postmenopausal women: a randomized controlled trial. Cancer Epidemiol Biomarkers Prev 2014; 23(4): 648-57.
25. Wynn T. Cellular and molecular mechanisms of fibrosis. J Pathol. 2008; 214(2):199-210.
26. Brixius K, Schoenberger S, Ladage D, Knigge H, Falkowski G, Hellmich M, et al. Long-term endurance exercise decreases antiangiogenic endostatin signalling in overweight men aged 50–60 years. BJSM 2008; 42(2): 126-9.
27. Suhr F, Rosenwick C, Vassiliadis A, Bloch W, Brixius K. Regulation of extracellular matrix compounds involved in angiogenic processes in short- and long-track elite runners. Scand J Med Sci Sports 2010; 20(3): 441-8.
28. Sponder M, Campean IA, Emich M, Fritzer-Szekeres M, Litschauer B, Graf S, et al. Long-term physical activity leads to a significant increase in serum sRAGE levels: a sign of decreased AGE-mediated inflammation due to physical activity? Heart Vessels 2018; 33(8): 893-900.
29. Nourshahi M, Farahmand F, Bigdeli MR. Effect of cinnamon-extract supplementation on VEGF and Endostatin level in hind leg muscle of aged rats after one session of exhaustive exercise. Iran J P P 2017; 1(3): 184-76.
30. Seida A, Wada J, Kunitomi M, Tsuchiyama Y, Miyatake N, Fujii M, et al. Serum bFGF levels are reduced in Japanese overweight men and restored by a 6-month exercise education. Int J Obes Relat Metab Disord 2003; 27(11): 1325-31.
31. Noris M, Morigi M, Donadelli R, Aiello S, Foppolo M, Todeschini M, et al. Nitric oxide synthesis by cultured endothelial cells is modulated by flow conditions. Circ Res. 1995; 76(4): 536-43.
32. Okada M, Oba Y, Yamawaki H. Endostatin stimulates proliferation and migration of adult rat cardiac fibroblasts through PI3K/Akt pathway. Eur J Pharmacol 2015; 750: 20-6.

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