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:: Volume 22, Issue 3 (Bimonthly 2018) ::
Feyz 2018, 22(3): 258-266 Back to browse issues page
The effect of cyclooxygenase-2 inhibition on pentylenetetrazole-induced seizure threshold in mice
Azam Mesdaghinia , Paria Khazaee , Azhdar Heydari *
Physiology Research ‍Center, Kashan, University of Medical Sciences, Kashan, I. R. Iran. , heydariazh@gmail.com
Abstract:   (2949 Views)
Background: Previous studies have shown the protective effect of cyclooxygenase (COX) enzyme in development of convulsions. However, involvement of COX-2 in the pathogenesis of epilepsy is not yet well-known. The present study was designed to investigate the effect of celecoxib and nimesulide (selective COX-2 inhibitors) on pentylenetetrazole (PTZ)-induced clonic seizure threshold in mice.
Materials and Methods: In this study, white male mice were randomly divided into 13 groups including control groups, solvent (Tween 80) and eleven experimental groups which received celecoxib (2.5, 5 and 10 mg/kg), nimesulide (2.5, 5 and 10 mg/kg), diazepam (0.1, 0.5 and 5 mg/kg), and combination of non-effective dose of diazepam with celecoxib or nimesulide. Pentylenetetrazol-induced clonic seizure threshold was assessed in all groups.
Results: Nimesulide (2.5 and 5 mg/kg), celecoxib (2.5 and 5 mg/kg), and diazepam (0.5 and 5 mg/kg) significantly increased the PTZ-induced seizure threshold compared with the solvent group (P<0.05). Also, only combination of sub-effective dose of diazepam (0.1 mg/kg) with celecoxib (2.5 mg/kg) showed a significant protective effect against PTZ-induced seizure threshold (P<0.01).
Conclusion: Findings of the current study show the possible role of COX-2 isoenzyme in the pathophysiology of epilepsy. It is possible that some COX-2 inhibitors such as celecoxib act through GABAergic neurons and reduce excitability by increasing GABA release. Also, the difference between the effects of celecoxib and nimesulide can be attributed to the effect of these two compounds on COX-1 and COX-2 expression.
Keywords: Seizure, Pentylenetetrazole, Cyclooxygenase-2, GABA
Full-Text [PDF 186 kb]   (701 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/05/8 | Accepted: 2018/06/17 | Published: 2018/08/4
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Mesdaghinia A, Khazaee P, Heydari A. The effect of cyclooxygenase-2 inhibition on pentylenetetrazole-induced seizure threshold in mice. Feyz 2018; 22 (3) :258-266
URL: http://feyz.kaums.ac.ir/article-1-3599-en.html

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Volume 22, Issue 3 (Bimonthly 2018) Back to browse issues page
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