:: Volume 22, Issue 2 (Bimonthly 2018) ::
Feyz 2018, 22(2): 222-229 Back to browse issues page
Evaluation of the prevalence of typical and atypical enteropathogenic Escherichia coli isolated from stool specimens of patients with diarrhea admitted to Tehran Children's Hospital by the PCR Method
Rashin Bahmanabadi , Mohammad Bagher Khalili , Bita Bakhshi , Mohammad Mehdi Soltan Dallal
Department of Food Microbiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, I. R. Iran. , msoltandallal@gmail.com
Abstract:   (3871 Views)
Background: Infectious diarrheal diseases are a major cause of death in community, especially in children. Enteropathogenic Escherichia coli (EPEC) are considered as a major cause of children's diarrhea in developing countries. The aim of this study was to evaluate the prevalence of both typical Enteropathogenic (tEPEC) and atypical Enteropathogenic (aEPEC) E. coli isolated from patients admitted to the children's hospital in Tehran by the PCR method.
Materials and Methods: In this cross-sectional study, a total of 157 children diarrheal samples were collected from February 2016 to August 2017 and were sent to the microbiology department in the School of Public Health in Tehran University of Medical Sciences for testing. The identification of isolates was performed by conventional biochemical tests. The typical and atypical E. coli isolates were identified for the presence of eae, sxt1, sxt2 genes, and bfp A by the PCR method. The drug resistance patterns of isolated EPEC were tested by the agar disk diffusion method. The antibiotics used were amoxicillin-clavulanic, ampicillin, gentamicin, trimethoprim- sulfamethoxazole, ciprofloxacin, cefepim, Nitrofurantoin and imipenem.
Results: Out of 101 E. coli isolates, 7 were identified as EPEC. All the isolated strains carried eae but not stx1, stx2, and bfp A genes. Also, 100% of the isolates were resistant to amoxicillin-clavulanic and ampicillin.
Conclusion: A high prevalence of EPEC in children can be considered as a threat to the children's health. In this study, all the isolates were aEPEC.
Keywords: Escherichia coli Enteropathogenic, Typical, Atypical, Children, Diarrhea, PCR
Full-Text [PDF 374 kb]   (1269 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/08/26 | Revised: 2018/06/7 | Accepted: 2018/02/26 | Published: 2018/03/19
References
1. Liu L, Johnson HL, Cousens S, Perin J, Scott S, E. Lawn J, et al. Global, regional,and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000. Lancet 2002; 379(9832): 2151–61.
2. Scaletsky IC, Fabbricotti SH, Silva SO, Morais MB, Fagundes Neto U. HEp-2-adherent Escheri‌chia coli strains associated with acute infantile diarrhea, Sao Paulo, Brazil. Emerg Infect Dis 2002; 8(8): 855–8.
3. Blanco M, E.Blanco J, Dahbi GH, P.Alonso M, Mora A, A.Coira M, et al. Identification of two new intimin types in atypical enteropathogenic Escheri‌chia coli. Int Microbiol 2006; 9(2): 103-10.
4. Castillo A, Eguiarte LE, Souza V. A genomic popu‌lation genetics analysis of the pathogenic enterocyte effacement island in Escherichia coli:the search for the unit of selection. Proc Natl Acad Sci U. S. A. 2005; 102(5): 1542–7.
5. Nazari Z, Moradli G, Bakhshi B. Molecular identification of Escherichia coli EPEC isolated from children under the age of 5 years by multiplex PCR in Kermanshah. Scien J Ilam Uni Med Sci 2016; 24(1): 154-61. [in Persian]
6. Santona S, Diaz N, Fiori PL, Francisco M, Sidat M, Cappuccinelli P, et al. Genotypic and pheno‌typic features of enteropathogenic Escherichia coli isolated in industrialized and developing countries. J Infect Dev Ctries 2013; 7(3): 214-9.
7. Hernandes RT, Elias WP, Vieira MA, Gomes TA. An overview of atypical enteropathogenic Escherichia coli. FEMS Microbiol Lett 2009; 297(2): 137–49.
8. Ochoa TJ, Barletta F, Contreras C, Mercado E. New insights into the epidemiology of entero‌patho‌genic Escherichia coli infection. Trans R Soc Trop Med Hyg 2008; 102(9): 852–6.
9. Afset JE, Bevanger L, Romundstad P, Bergh K. Association of atypical enteropathogenic Escheri‌chia coli with prolonged diarrhoea. J Med Microbiol 2004; 53(11): 1137–44.
10. Nguyen RN, Taylor LS, Tauschek M, Robins-Browne RM. Atypical enteropathogenic Escheri‌chia coli infection and prolonged diarrhea in children. Emerg Infect Dis 2006; 12(4): 597–603.
11. Blanco M, Blanco JE, Dahbi G, Mora A, Alonso MP, Varela G, et al. Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (muB and xiR/beta2B). J Med Microbiol 2006; 55(9): 1165-74.
12. Wong Fok Lung T, Pearson JS, Schuelein R, Schuelein R L. Hartland E. The cell death response to enteropathogenic Escherichia coli infection‌. Wiley Online Library 2014‌; 16(12): 1736–45.
13. Tuite N, Reddington K, Barry T, Zumla , Enne V. Rapid nucleic acid diagnostics for the detection of antimicrobial resistance in Gram-negative bac‌teria: is it time for a paradigm shift? J Antimicrob Chemother 2014; 69(7): 1729-33.
14. Wayne PA. Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Anti‌microbial Susceptibility Testing, CLSI supplement M100S, 26th ed. Clinical and Laboratory Standards Institute., Wayne, PA, 2016.
15. Najibi S, Bakhshi B, Fallahzad S, Pourshafie M, Katouli M, Sattari M, et al. Distribution of `class 1 integrons among enteropathogenic Escherichia coli. Can J Microbiol 2012; 58(5): 637-43.
16. Singh T, Das S, Ramachandran VG, Wani S, Shah D, Maroof KA, Sharma A. Distri bution of Integrons and Phylogenetic Groups among Entero‌pathogenic Escherichia coli Isolates from Children<5 Years of Age in Delhi, India. Front Microbiol 2017; 8: 561.
17. Jafari F, Garcia-Gil LJ, Salmanzadeh-Ahrabi S, Shokrzadeh L, Aslani M, Pourhoseingholi M, et al. Diagnosis and prevalence of enteropathogenic bacteria in children less than 5 years of age with acute diarrhea in Tehran children's hospitals. J Infect 2009; 58(1): 21-7.
18. Nataro JP, Baldini MM, Kaper JB, Black RE, Bravo N, Levine MM. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. J Infecti Dis 1985; 152(3): 560-5.
19. Zali Mr, Moez Ak, Parcham Ak, Nik-Kholgh B. Etiologies of acute diarrheal diseases in Iran. J Res Med Sci 2003; 7(4): 346-56.
20. Soltan Dallal MM. Diarrhea caused by entero‌pathogenic bacteria in children. Arch Iran Med 2001; 4(4): 201-321.
21. Nasrollahi M, Sharifi M. Prevalence of dia‌rrhea caused by Enteropathogenic E.coli in children less than one year in sari. J SSU 1999; 793: 26-30. [in Persian]
22. Alikhani MY, Mirsalehian A, Aslani MM. Detection of typical and atypical enteropathogenic Escherichia coli (EPEC) in Iranian children with and without diarrhoea. J Med Microbiol 2006; 55(Pt 9): 1159-63.[in Persian]
23. Motallebi M, Piroozmand A, Rohani M, Akbari H, Khorshidi A. Multiple drug resistance of entero‌pathogenic Escherichia coli isolated from children with diarrhea in Kashan, Iran. African JMR 2011; 5(20): 3305-9.
24. Pereira ACM, Britto-Filho JD, José de Carvalho J, de Luna MdG, Rosa ACP. Entero‌aggregative Escherichia coli (EAEC) strains enter and survive within cultured intestinal epi‌thelial cells. Microbial Path 2008; 45(5-6): 310-4.
25. Blanco M, Blanco JE, Dahbi G, Alonso MP, Mora A, Coira MA, et al. Identification of two new intimin types in atypical enteropathogenic Escherichia coli. Int Microbiol 2006; 9(2): 103-10.
26. Albert MJ, Rotimi VO, Dhar R, Silpikurian S, Pacsa AS, Molla AM, et al. Diarrhoeagenic Esche‌ri‌chia coli are not a significant cause of diarrhoea in hospitalised children in Kuwait. BMC Microbiol 2009; 9(1): 62.
27. Kalantar E, Soheili F, Salimi H, Soltan Dallal MM. Frequency, antimicrobial susceptibility and plasmid profiles of Escherichia coli pathotypes obtained from children with acute diarrhea. J Jundishapur Microbiol 2011; 4(1): 23-8.
28. Ochoa TJ, Contreras CA. Enteropathogenic escherichia coli infection in children. Curr Opin Infect Dis 2011; 24(5): 478-83.
29. Dutta S, Guin S, Ghosh S, Pazhani GP, Rajendran K, Bhattacharya MK, et al. Trends in the prevalence of diarrheagenic Escherichia coli among hospitalized diarrheal patients in Kolkata, India. PloS One 2013; 8(2): e56068.

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Volume 22, Issue 2 (Bimonthly 2018) Back to browse issues page