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:: Volume 26, Issue 3 (Bimonthly 2022) ::
Feyz 2022, 26(3): 247-255 Back to browse issues page
The effect of aerobic exercise, ozone and mesenchymal stem cells on pain threshold changes in osteoarthritis rats
Leila Abdizadeh , Maghsoud Peeri * , Hasan Matinhomaee
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, I.R. Iran. , m.peeri@iautcb.ac.ir
Abstract:   (473 Views)
Background: Acute and chronic stress due to inactivity, especially inactivity due to osteoarthritis can affect pain threshold and painful behaviors; therefore, this study aimed to determine the effect of aerobic exercise, ozone and MSCs on pain threshold changes in osteoarthritis rats.
Materials and Methods: Forty-five adult male Wistar rats (6-8-week-old), weighing an average of 250-230g, were divided into osteoarthritis (n=40) and healthy controls (n=5). Osteoarthritis were induced by surgery in rats and then divided into 8 groups of 5, including patient control, MSCs, aerobic exercise, ozone, aerobic exercise+ozone, aerobic exercise+MSCs, ozone+MSCs and aerobic exercise+ozone+MSCs. Aerobic exercise was performed for eight weeks at a speed of 16 M/min. MSCs were injected at 1×106 cells/kg into the knees of rats; ozone was also injected into 3 replicates before training with a concentration of 20 μg/ml. Pain threshold was measured by hot-plate test and its significance level was considered 0.05.
Results: Pain threshold in the osteoarthritis patient group was significantly reduced compared to the healthy group (P=0.001). There was a significant increase in pain threshold in cell therapy (P=0.003), ozone therapy and aerobic exercise groups (P=0.0001). While the peak of this increase was seen in MSCs+aerobic exercise (P=0.02).
Conclusion: Induction of osteoarthritis leads to a decrease in pain threshold. Although aerobic exercise, ozone and MSCs play an important role in controlling acute pain due to osteoarthritis by increasing the pain threshold, the maximum analgesic effect is in the interaction of aerobic exercise+MSCs.
Keywords: Exercise, MSCs, Ozone, Hot-Plate, Osteoarthritis, Pain threshold
Full-Text [PDF 442 kb]   (263 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/03/8 | Accepted: 2022/06/26 | Published: 2022/07/30
1. Thomas E, Peat G, Mallen C, Wood L, Lacey R, Duncan R, et al. Predicting the course of functional limitation among older adults with knee pain: do local signs, symptoms and radiographs add anything to general indicators? Ann Rheum Dis 2008; 67(10): 1390-8.
2. Ford GK, Finn DP. Clinical correlates of stress-induced analgesia: evidence from pharmacological studies. Pain 2008; 140(1): 3-7.
3. McCarberg BH, Billington R. Consequences of neuropathic pain: quality-of-life issues and associated costs. Am J Manag Care 2006; 12(9): 263-8.
4. Mohammadi F, Mohammadkhani P, Dolatshahi B, Asghari MA. The effects of “mindfulness meditation for pain management” on the severity of perceived pain and disability in patients with chronic pain. Sija 2011; 6(1): 59-66. [in Persian]
5. Yuan XC, Zhu B, Jing XH, Xiong LZ, Wu CH, Gao F, et al. Electroacupuncture potentiates cannabinoid receptor-mediated descending inhibitory control in a mouse model of knee osteoarthritis. Front Mol Neurosci 2018; 11(112): 1-15.
6. Syx D, Tran PB, Miller RE, Malfait A-M. Peripheral mechanisms contributing to osteoarthritis pain. Curr Rheumatol Rep 2018; 20(2): 9.
7. Farhang D, Talebi N, Hasan pour Ezzati M. Comparison of the effects of aerobic and resistance training on changes in inflammatory pain threshold in male rats. Feyz 2021; 25(3): 987-93. [in Persian]
8. Burrows NJ, Booth J, Sturnieks DL, Barry BK. Acute resistance exercise and pressure pain sensitivity in knee osteoarthritis: a randomised crossover trial. Osteoarthr Cartil 2014; 22(3): 407-14.
9. Juhl C, Christensen R, Roos EM, Zhang W, Lund H. Impact of exercise type and dose on pain and disability in knee osteoarthritis: a systematic review and meta‐regression analysis of randomized controlled trials. Arthrit Rheumatol 2014; 66(3): 622-36.
10. Jones MD, Booth J, Taylor JL, Barry BK. Aerobic training increases pain tolerance in healthy individuals. Med Sci Sports Exerc 2014; 46(8): 1640-7.
11. Østerås H, Paulsberg F. The effect of medical exercise therapy on pressure sensitivity in patients with knee osteoarthritis: a cohort pilot study. Pain Ther 2019; 8(1): 79-87.
12. Cuadros ME, Moro OS, Florin MJ, Canelo JA. Ozone improves pain, function and quality of life in patients with knee osteoarthritis: A prospective quasi-experimental before-after study. Middle East J Rehabil Health 2017; 4(1): 418-21.
13. Saad SI, Elrabiey MA, Elazzazi HM, Shaboob EA, Elbagoury ZM. Comparison of efficacy of radiofrequency alone or steroid or ozone intra-articular after radiofrequency ablation of genicular nerves in patients with chronic pain due to knee osteoarthritis: A prospective, randomized, controlled clinical trial. Benha J Appli Sci 2021; 6(6): 119-25.
14. Wang J, Zhou L, Zhang Y, Huang L, Shi Q. Mesenchymal stem cells-a promising strategy for treating knee osteoarthritis: a meta-analysis. Bone Joint Res 2020; 9(10): 719-28.
15. Schäfer S, Berger JV, Deumens R, Goursaud S, Hanisch UK, Hermans E. Influence of intrathecal delivery of bone marrow-derived mesenchymal stem cells on spinal inflammation and pain hypersensitivity in a rat model of peripheral nerve injury. J Neuroinflammation 2014; 11(1): 1-5.
16. Kaya A, Sonmez M, Kar T, Haholu A, Yildirim Y, Müftüoğlu T, et al. Efficiency of ozone therapy in a rat model of experimental uveitis. Ocul Immunol Inflamm 2017; 25(5): 700-5.
17. Bove SE, Laemont KD, Brooker RM, Osborn MN, Sanchez BM, Guzman RE, et al. Surgically induced osteoarthritis in the rat results in the development of both osteoarthritis-like joint pain and secondary hyperalgesia. Osteoarthr Cartil 2006; 14(10): 1041-8.
18. Liu SS, Zhou P, Zhang Y. Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis. Mol Med Rep 2016; 13(3): 1999-2006.
19. Rezaie M, Azarbayjani MA, Peeri M, Hosseini SA. The effect of aerobic exercise with ozone and stem cell on anandamide concentration in desert rat with osteoarthritis. Feyz 2022; 26(1): 38-46. [in Persian]
20. Li M, Luo X, Lv X, Liu V, Zhao G, Zhang X, et al. In vivo human adipose-derived mesenchymal stem cell tracking after intra-articular delivery in a rat osteoarthritis model. Stem Cell Res Ther 2016; 7(1): 1-3.
21. Lopes de Jesus CC, Dos Santos FC, de Jesus LM, Monteiro I, Sant’Ana MS, Trevisani VF. Comparison between intra-articular ozone and placebo in the treatment of knee osteoarthritis: A randomized, double-blinded, placebo-controlled study. PloS One 2017; 12(7): 179-85.
22. Zarmehri HA, Haidari-Oranji N, Soleimani NE, Sofiabadi MO. Effects of lidocaine injections into the rostral ventromedial medulla on nociceptive behviours in hot-plate and formalin tests in rats. Koomesh 2013; 14(4): 490-96. [in Persian]
23. Kuni B, Wang H, Rickert M, Ewerbeck V, Schiltenwolf M. Pain threshold correlates with functional scores in osteoarthritis patients. Acta Orthopaedica 2015; 86(2): 215-9.
24. Shetty YC, Singh VK, Manjesh PS, Nagarajan VB, Patil P, Chawda M, et al. Evaluation of the effect of an ayurvedic formulation Myostaal Forte tablets on chondroprotective biomarkers in an experimental model of osteoarthritis in rats. Phytomedicine Plus 2021;1(3):1000-82.
25. Cobianchi S, Marinelli S, Florenzano F, Pavone F, Luvisetto S. Short-but not long-lasting treadmill running reduces allodynia and improves functional recovery after peripheral nerve injury. Neurosci 2010; 168(1): 273-87.
26. Cunha CO, Pinto-Fiamengui LM, Sampaio FA, Conti PC. Is aerobic exercise useful to manage chronic pain? Revista Dor 2016; 17(1): 61-4.
27. Naugle KM, Naugle KE, Fillingim RB, Samuels B, Riley III JL. Intensity thresholds for aerobic exercise–induced hypoalgesia. Med Sci Sports Exerc 2014; 46(4): 817.
28. Naugle KM, Fillingim RB, Riley III JL. A meta-analytic review of the hypoalgesic effects of exercise. J pain 2012; 13(12): 1139-50.
29. Mishra SK, Pramanik R, Das P, Das PP, Palit AK, Roy J, et al. Role of intra-articular ozone in osteo-arthritis of knee for functional and symptomatic improvement. Ind J Phys Med Rehabilit 2011; 22(2): 65-9.
30. Xu W, Zhao X, Sun P, Zhang C, Fu Z, Zhou D. The effect of medical ozone treatment on cartilage chondrocyte autophagy in a rat model of osteoarthritis. Am J Transl Res 2020; 12(9): 59-67.
31. Hashemi M, Nabi BN, Saberi A, Sedighinejad A, Haghighi M, Farzi F, et al. The comparison between two methods for the relief of knee osteoarthritis pain: Radiofrequency and intra-periarticular ozone injection: A clinical trial study. Int J Med Res Health Sci 2016; 5(7): 539-46.
32. Calunga JL, Menéndez S, León R, Chang S, Guanche D, Balbín A, Zayas J, García P. Application of ozone therapy in patients with knee osteoarthritis. Ozone: Sci Engineering 2012; 34(6): 469-75.
33. Lopa S, Colombini A, Moretti M, de Girolamo L. Injective mesenchymal stem cell-based treatments for knee osteoarthritis: from mechanisms of action to current clinical evidences. Knee Surg Sports Traumatol Arthrosc 2018; 27(6): 2003-20.
34. Hua Z, Liu L, Shen J, Cheng K, Liu A, Yang J, et al. Mesenchymal stem cells reversed morphine tolerance and opioid-induced hyperalgesia. Scientific reports 2016; 6(1): 1-13.
35. Gupta PK, Chullikana A, Rengasamy M, Shetty N, Pandey V, Agarwal V, et al. Efficacy and safety of adult human bone marrow-derived, cultured, pooled, allogeneic mesenchymal stromal cells (Stempeucel®): preclinical and clinical trial in osteoarthritis of the knee joint. Arthritis Res Ther 2016; 18(1): 1-8.
36. Matas J, Orrego M, Amenabar D, Infante C, Tapia-Limonchi R, Cadiz MI, et al. Umbilical cord-derived mesenchymal stromal cells (MSCs) for knee osteoarthritis: repeated MSC dosing is superior to a single MSC dose and to hyaluronic acid in a controlled randomized phase I/II trial. Stem Cells Transl Med 2019; 8(3): 215-24.
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Abdizadeh L, Peeri M, Matinhomaee H. The effect of aerobic exercise, ozone and mesenchymal stem cells on pain threshold changes in osteoarthritis rats. Feyz 2022; 26 (3) :247-255
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Volume 26, Issue 3 (Bimonthly 2022) Back to browse issues page
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