Effect of Eight Weeks of Capsaicin Supplementation on the Expression of Runx2 and ALP Genes in Bone Tissue of Aged Male Wistar Rats

Varzandeh, Vajihe; Kazemzadeh, Yaser; Mirzayan Shanjani, Sanaz; Shirvani, Hossein

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Effect of Eight Weeks of Capsaicin Supplementation on the Expression of Runx2 and ALP Genes in Bone Tissue of Aged Male Wistar Rats

Vajihe Varzandeh

, Yaser Kazemzadeh ^*

, Sanaz Mirzayan Shanjani

, Hossein Shirvani

Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran , yaser.kazemzadeh@yahoo.com

Abstract: (162 Views)

Background and Aim: Capsaicin, known for its anti-inflammatory and antioxidant properties, plays a critical role in regulating the expression of genes associated with the health of various tissues, including bone. This study aimed to evaluate the effect of eight weeks of capsaicin supplementation on the expression of Runx2 and ALP genes in the bone tissue of aged male Wistar rats.
Methods: In this experimental study, 16 aged male Wistar rats were randomly assigned to two groups: capsaicin supplementation (n=8) and control (n=8). The supplementation group received capsaicin at a dose of 5 mg/kg body weight via oral gavage for eight weeks. Forty-eight hours after the final supplementation dose, the rats were anesthetized with intraperitoneal ketamine and xylazine and subsequently euthanized. Femoral bone tissue samples were collected under sterile conditions, and the expression of Runx2 and ALP genes was assessed using RT-PCR.
Results: Runx2 gene expression was significantly increased in the capsaicin supplementation group compared to the control group (fold change = 3.69%, P=0.038). In contrast, ALP gene expression showed a non-significant increase in the supplementation group (fold change = 4.28%, P=0.029).
Conclusion: Eight weeks of capsaicin supplementation significantly upregulated Runx2 gene expression in the bone tissue of aged rats. Given the pivotal role of Runx2 in osteogenesis, these findings suggest that capsaicin may contribute to improved bone mass in aged rats by enhancing Runx2 expression. Further studies are warranted to confirm these results.

Keywords: Capsaicin, Runx2, ALP, Bone Tissue, Aging

Type of Study: Research | Subject: General
Received: 2025/02/24 | Revised: 2025/08/12 | Accepted: 2025/07/16

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