2024-03-28T19:39:02+03:30 http://feyz.kaums.ac.ir/browse.php?mag_id=83&slc_lang=fa&sid=1
83-2462 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The effect of new palladium (II) complexes of dithiocarbamate derivatives on the morphology and clonogenicity of liver cancer cell line Shahram Hadizadeh Mohammad Mazani m.mazani@arums.ac.ir Noroz Najafzadeh Hasan Mansouri-Torshizi Bagher Pourheydar Hafez Mirzanejad-Asl Background: While today different drugs have been used to treat cancer, none of them have proved to be effective. Heavy metal complexes (e.g., platinum and palladium) can be useful in the treatment of cancer. The purpose of this study was to evaluate the effects of newly synthetized palladium complexes on the morphology and clonogenicity of liver cancer cell line (HepG2). Materials and Methods: New palladium complexes were synthesized in the chemistry lab, University of Sistan and Baluchesta. The cytotoxicity and anti-cancer effects of newly synthesized complexes were evaluated using the clonogenic and cell death assay (apoptosis and necrosis) using acridine orange/ ethidium bromide double staining.Results: The results of this study showed that the new complexes had potential concentration-dependent effects on HepG2 cell line. There was a significant difference between the number of colonies formed after treatment with different concentrations of palladium complexes (0.5, 1, 2, 4 and 8 µg/ml) compared to those in the control group (P<0.01). Furthermore, the cells stained with acridine orange/ethidium bromide indicated the occurrence of apoptosis in cancer cells after the treatment with various concentrations of palladium complexes. Conclusion: The results of this study show the anti-cancer effect of palladium complexes on HepG2 cell lines. Also, the palladium complexes can reduce the number of cell colonies and cause cell death by apoptosis. HepG2 DNA Apoptosis Necrosis 2015 1 01 506 514 http://feyz.kaums.ac.ir/article-1-2462-en.pdf
83-2464 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The effect of curcumin on the estradiol valerate-induced polycystic ovary in rats Mohammad Nabiuni devbiokharazmi@gmail.com Shima Mohammadi Parvin Kayedpoor Latifeh Karimzadeh Background: Polycystic ovary syndrome (PCOS) is a syndrome with complex endocrine and metabolic disorders, which is characterized by chronic anovulation, polycystic ovary and hyperandrogenism. Curcumin is a substance with antioxidant and anti-inflammatory effects. This study aimed to examine the therapeutic effect of curcumin on polycystic ovary syndrome in adult rats. Materials and Methods: In this experimental study, PCOS was induced in adult female Wistar rats (170 g) by subcutaneous injection of estradiol valerate (2 mg/kg). The control group received no injection. At the end of 60th day, the rats were divided into control, PCOS and curcumin-treated PCOS (100, 200, 300 and 400 mg/kg) groups. After 14 days of intraperitoneal curcumin treatment, blood and ovary samples of all groups were taken for histological and serological studies. Results: The thickness of the theca layer, primordial follicles and number of cysts were significantly decreased in high-dose curcumin treated ovaries compared to the PCOS group. Moreover, the appearance of corpus luteums as the main sign of recurrent ovulation was established. The serological analyses showed an increase in FSH and progesterone and a decrease in LH, estradiol and testosterone compared to the PCOS. Conclusion: The results indicate that curcumin may be a useful agent for improving the PCOS and initiation of ovulation through its various antioxidant and anti-inflammatory effects. Polycystic ovary syndrome Estradiol Corpus luteum Curcumin 2015 1 01 515 523 http://feyz.kaums.ac.ir/article-1-2464-en.pdf
83-2471 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The hemostatic effect of aluminum sulfate in liver bleeding in rat Saeed Nouri Mohammad Reza Sharif dr.mrsharif@yahoo.com Mehrdad Hosseinpour Hasan Ehteram Background: Despite great advances in the field of surgery, the control of liver parenchymal hemorrhage is still one of the challenges surgeons face in saving the patients’ lives. Aluminum sulfate is used as a hemostatic agent in dental surgery. This study aimed to examine the hemostatic effect of aluminum sulfate on controlling bleeding from liver parenchymal tissue. Materials and Methods: In this experimental study, 60 male Wistar rats were randomly allocated into six equal groups of 10 mice each. An incision ( 2 cm length 0.5 cm depth) was made on each rat's liver surface and the length of time to hemostasis as a dependent variable was measured using different concentrations of aluminum sulfate (5%, 10%, 15%, 25%, and 50%) against the control method (control of bleeding by suturing) as the independent variable. The liver tissue was examined for pathological changes. Results: The length of time to hemostasis in the aluminum sulfate groups was significantly less than that of the control group (P<0.01). The pathologic examination showed the highest frequency of low-grade inflammation based on the defined pathological grading. Conclusion: Aluminum sulfate is an effective hemostatic agent with low-grade inflammation effect in controlling the bleeding from liver parenchymal tissue in an animal model. Hemostasis Aluminum sulfate Liver Rat 2015 1 01 524 530 http://feyz.kaums.ac.ir/article-1-2471-en.pdf
83-2465 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The role of nitric oxide and opioid receptors in antispasmodic activity of Petroselinum crispum (parsley) seed extract on rat ileum Ferial Savary Feryal.savary@yahoo.com Ahmad Ali Moazedi Mohammad Kazem Gharibnaseri Mohammad Reza Zadkarami Background: Parsley seed inhibits rat ileum contraction. In the rat intestine, Nitric oxide (NO) has been suggested to participate in nonadrenergic noncholinergic (NANC) relaxation of longitudinal smooth muscle. In addition, endogenous opioid peptides and opiate drugs affect a variety of gastrointestinal (GI) functions, including motility. Studies have shown that opioids inhibit electrically induced smooth muscle contractions. The aim of present study was to investigate the involvement of opioid receptors and NO in antispasmodic activity of parsley seed hydroalcoholic extract. Materials and Methods: In this study, 28 rats were randomly divided into 4 equal groups: 1) the effect of different concentrations of extract on KCl-induced contraction, 2) the effect of L-NAME on extract function, 3) the effect of naloxone on extract function, 4) the long-term effect of KCl. A piece of terminal portion of male rat ileum (2 cm) was dissected out and mounted in an organ bath containing air bubbled Tyrode solution (37 οC, pH=7.4) with 1 g initial tension and ileal contractions induced by KCl was recorded by an isotonic transducer. Antispasmodic activity of extract after tissue incubation was evaluated using Nω-nitro-L-arginine methyl ester (L-NAME) and naloxone. Results: The spasmolytic effect of the cumulative concentrations of the extract (0.1-0.8 mg/ml) was not reduced sensibly after tissue incubation with L-NAME (100 μM, 30 min) as a nitric oxide synthase inhibitor and naloxone (1µM, 30 min) as a non-selective opioid receptor antagonist. Conclusion: Although Parsley seed extract inhibit rat ileum contraction, it seems that NO and opioid receptors are not involved in this activity. Parsley Nitric oxide Opioid receptors Ileum Antispasmodic 2015 1 01 531 538 http://feyz.kaums.ac.ir/article-1-2465-en.pdf
83-2467 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 Comparing the effects of the aqueous extract of Aloe vera and Na+ Nitroprusside on the contraction of rat isolated thoracic aorta Fatemeh Khodaei Azam Mesdaghinia Gholam Ali Hamidi Mehdi Noureddini mnoureddini@kaums.ac.ir Background: Aloe vera is used in traditional medicine to treat hypertension. The purpose of this study was to examine the effect of the aqueous extract of Aloe vera (compared to Na+ nitroprusside) on the contraction of the rat isolated thoracic aorta precontracted with 20 mM potassium chloride. Materials and Methods: In this study, 18 rings isolated from the thoracic aorta of 9 Wistar rats (250-300g) were divided into three groups. On the loaded tissue, a tension less than 2 g was applied which then was precontracted with potassium chloride (20mM) in organ bath containing the carbogen-Krebs solution. Changes in aorta contractility in response to cumulative application of vehicle (group I), extract (group II: 5-50 mg/ml) and Na+ Nitroprusside (group III: 0.0001-10 μM) were measured isometrically.Results: The results showed that although Aloe vera has a decreasing effect on the contraction induced by potassium chloride (20mM), this effect was less than the cumulative effect of Na+ nitroprusside (P<0.05). Conclusion: The possible antihypertensive mechanism of Aloe vera may be for reducing the contractility of vascular smooth muscle and this effect is weaker than the effect of nitroprusside. Hence, Aloe vera can be used as a potential drug for lowering the high blood pressure. Thoracic aorta Isolated tissue Rat Aqueous extract Aloe vera Na+ nitroprusside 2015 1 01 539 545 http://feyz.kaums.ac.ir/article-1-2467-en.pdf
83-2470 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The role of β-adrenergic receptors in the antispasmodic effect of flavonoid from Petroselinum crispum (parsley) leaf on adult male rat ileum Azam Govahi Azam.govahi@yahoo.com Ahmad Ali Moazedi Seyyed Mansoor Seyyednejad Mehdi Poormehdi-Broojeni Background: Petroselinum crispum (parsley) has received much attention in recent researches for its flavonoid content. Former studies have shown that flavonoids can suppress the contraction of the ileum induced by KCl and acetylcholine. This study aimed to examine the role of the beta-adrenergic receptors in the antispasmodic effect of parsley leaf extract on smooth muscle contraction of rat ileum.Materials and Methods: In this study, a 2-cm segment of terminal ileum from male Wistar rats was isolated and suspended in organ bath containing Tyrode's solution under a 1g tension and contractions were recorded by an isotonic transducer. Rats were divided into two groups of seven each. The spasmolytic effect of the cumulative concentration of the extract was investigated after tissue incubation with propranolol as a β-adrenergic antagonist. Results: The extract of parsley leaves reduced the ileum contraction induced by KCl (60mM) in a dose dependent manner (P<0.001). Moreover, the antispasmodic effect of flavonoids in the extract at a concentration of 16 mg/ml was influenced and significantly decreased by propranolol.Conclusion: Parsley leaf extract at higher concentrations has a relaxing effect on rat ileum and this effect is mediated via β-adrenergic receptors. Flavonoid Ileum Contraction β-adrenergic receptors 2015 1 01 546 552 http://feyz.kaums.ac.ir/article-1-2470-en.pdf
83-2468 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 Investigating the human hemoglobin fructation in the presence of propolis in vitro Yonos Sahebi Adeleh Divsalar divsalar@khu.ac.ir Ali Akbar Saboury Background: Propolis is a complex resinous mixture that is gathered and processed by honeybees from resin they collect from trees and plants. This substance has various biological properties. Glycation is a reaction, which occurs between a protein and a reducing sugar and finally causes structural alterations and destruction of proteins. The role of glycation has been approved in the development and aggravation of diabetic complications. This study aimed to examine the effect of ethanolic extract of propolis (EEP) on fructation and destruction of hemoglobin protein structure. Materials and Methods: In this experimental study, purified hemoglobin was incubated alone and with fructose in the presence and absence of different concentrations of EEP (10, 20 and 40µg/ml) for 5 weeks. The extent of hemoglobin fructation was determined by measuring the amount of heme release, blue shift in soret band, releasing the products of heme destruction and assessing the amyloid structures using the UV-visible and fluorescence spectroscopy. Results: Incubation of hemoglobin with fructose was led to the hemoglobin destruction and heme release. Hemoglobin fructation was inhibited up to 45% in the presence of EEP with a concentration of 40µg/ml. The two lower concentrations of EEP showed the lower degrees of inhibition. Moreover, fluorescence studies of products resulting from heme degradation and fibrillar structures are indicative of the reduction in hemoglobin fructation in the presence of EEP. Conclusion: Hemoglobin is drastically glycated in the presence of fructose and EEP can decrease the hemoglobin fructation in a concentration-dependent manner. Fructation Hemoglobin Propolis Antioxidant 2015 1 01 553 563 http://feyz.kaums.ac.ir/article-1-2468-en.pdf
83-2469 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The effect of Ginger powder on the elimination of Acetaminophen-induced renal toxicity in adult male rats Malihe Morshedi Ali Gol Background: Acetaminophen has been recognized as an analgesic, but its overuse can cause renal and hepatic toxicity. Ginger as a spice has been used in traditional medicine since the year 2000 in China and Iran. The purpose of the present study was to evaluate the effect of Ginger on renal function in toxicity induced by Acetaminophen. Materials and Methods: This experimental study was performed on 24 male Wistar rats. After 24 hours of fasting, animals were allocated into four groups as follows: the control and Ginger groups each receiving normal saline the Acetaminophen and Acetaminophen+Ginger groups each receiving Acetaminophen (1000 mg/kg) via the gavage. After eight hours, the control and Acetaminophen groups received normal saline and the Ginger and Acetaminophen+Ginger groups received Ginger powder (500 mg/kg). After twelve hours, animals were sacrificed and blood samples were taken to evaluate the serum urea and creatinine. Moreover, the measurement of creatinine in the urine was done.Results: Serum creatinine levels were increased and decreased in the Acetaminophen group (P<0.01) and urine (P<0.001), respectively. In the Acetaminophen+Ginger group, the serum creatinine was decreased (P<0.05) and its excretion in the urine was increased. Creatinine clearance was decreased in the Acetaminophen group (P<0.05) however, it was increased in the Acetaminophen+Ginger group. Conclusion: Ginger results in the improvement of renal function in Acetaminophen-induced nephrotoxicity. Acetaminophen Ginger Kidney Rat 2015 1 01 564 570 http://feyz.kaums.ac.ir/article-1-2469-en.pdf
83-2466 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The effect of balance training and pilates on static and functional balance of elderly men Morteza Aradmehr mortezaaradmehr@yahoo.com Ali Sagheeslami Saeed Ilbeigi Background: Physiological changes related to aging and muscular disorders can increase the risk of falling, as a major problem of elderly group which in cases may even cause death. The purpose of this study was to compare the effects of a 6-week pilates and balance training program on static and functional balance among the elderly. Materials and Methods: In this quasi-experimental study, 30 elderly people selected from Kashmar (Khorasan province, Iran) were randomly divided into three groups: the balance training, pilates and control groups. The body-mass index and the static balance stork test were used in all three groups. The static and functional balance assessments were performed using the stork and timed up and go tests, respectively. All tests were repeated for the groups after six weeks of training (three sessions per week) in the same condition.Results: The mean values for the static and functional balance time were 5.51±0.55 and 10.71±6.72 before 4.42±0.90 and 28.42±8.88 after the balance training, respectively which showed a significant improvement (P=0.001vs P=0.023). However, the pilates training significantly improved only the static balance (P=0.027). Conclusion: It can be concluded that six weeks of balance training can improve both static and functional balance in the elderly men, while the pilates exercise can only improve the static balance of the elderly. Pilates Static balance Postural stability 2015 1 01 571 577 http://feyz.kaums.ac.ir/article-1-2466-en.pdf
83-2473 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 Polypharmacy and its related factors among the elderly population in Kashan, Iran during 2011-2012 Mansoor Dianati mandianati@yahoo.com Gholam Ali Shojaegharebag Azam Mesdaghinia Mohsen Taghadosi Faezeh Shenasa Atefeh Taiebi Mahbobeh Hafezi Somayeh Salehi Background: Elderly individuals use more drug and they are at a higher risk of polypharmacy complications. This study aimed to evaluate the polypharmacy and its related factors among the elderly group in Kashan (Iran) during 2011-2012. Materials and Methods: In a cross-sectional study, 360 elderly people over 60 years old were selected using the cluster-randomized method. Data were gathered based on a questionnaire contained demographic variables (e.g. age, sex, marital status, income status, level of education, hospitalization history) and main variables (e.g. the name and number of medications used, duration of use, the number of physicians prescribed drug, the number of potentially inappropriate medication use in elderly due to their background diseases and the number of potentially inappropriate medication used in an elderly group using the 2000 Beers criteria.Results: A total of 308 (88%) elderly (170 women and 138 men) had taken at least one medication during the interview. Mean and range of age of the elderly were 70.8± 7.9 and 60-100 years, respectively. Mean and range of medications were 6.2±3.53 and 1-23 respectively had taken by among individuals older than age 60 in kashan. Prevalence of polypharmacy in related population was 54.5 % (167 cases) and 66 elderly people (21.4%) at least took one inappropriate drug among their medications. Conclusion: Polypharmacy among the Kashan elderly population is relatively high. Therefore, to decrease the number of prescribed medications and their related side effects in elderly individuals, applying up to date criteria in prescribing the medication for aged population is recommended. Elderly Polypharmacy Related factors 2015 1 01 578 584 http://feyz.kaums.ac.ir/article-1-2473-en.pdf
83-2463 2024-03-28 10.1002
Feyz Medical Sciences Journal Feyz 1029-7855 2008-9821 2015 18 6 The effect of a single bout of severe aerobic exercise on apolipoproteins A, B and some serum lipid profiles Mohammad Javad Pourvaghar Alireza Shahsavar Mohammad Ebrahim Bahram m.bahram@spr.ui.ac.ir Background: Cardiovascular diseases cause extensive deaths throughout the world because of the increase in the concentration of cholesterol in blood plasma in the form of low-density lipoproteins (LDL). This study aimed to examine whether a single bout of exhaustive aerobic physical activity has a significant positive effect on apolipoproteins A and B, LDL and high-density lipoprotein (HDL) levels. Materials and Methods: In this study, 26 male college students were randomly assigned to the experimental (n=13) and control (n=13) groups. The experimental group further performed 20 minutes of running exercise with their highest speed and power in an indoor gymnasium. Blood samples were collected three times during the exercise. The first sample was collected prior to the start of the exercise. The second and third blood samples were collected immediately and 24 hours after the termination of the exercise. Blood sample in the control group was collected only in the first stage and fasting state. Results: There was a significant difference between the mean serum apolipoproteins A and B following the first stage and after the termination of the exercise (P=0.001). Moreover, a significant difference was observed between the mean serum triglyceride concentration of the subjects (P=0.001) and the mean serum concentration of HDL-C and LDL-C in the first and second stages (P=0.0001). Conclusion: It can be concluded that even a single bout of aerobic exercise performed with high density lasting 20 minutes can lead to a significant change in mean values of apolipoproteins A and cholesterol with high density as well as other serum lipid variables. Apolipoprotein A Apolipoprotein B High and low density lipoproteins Intense aerobic training 2015 1 01 585 591 http://feyz.kaums.ac.ir/article-1-2463-en.pdf