Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
The effect of treadmill exercise on catalepsy from reserpine-induced Parkinson model in diabetic male rat
397
404
FA
Azam
Khalaj
Department of Physiology, Qom Branch, Islamic Azad University, Qom, I. R. Iran.
Ramesh
Ahmadi
Department of Physiology, Qom Branch, Islamic Azad University, Qom, I. R. Iran.
Background: Parkinson's disease is a neurodegenerative disease of central nervous system, characterized by dopaminergic neuronal loss in the nigrostriatal pathway with clinical symptoms (e.g. resting tremor, rigidity, akinesia and disturbances in postural reflex). Diabetes as a metabolic disease has diverse complications (e.g. hyperglycemia) that may affect the severity of Parkinson symptoms. The aim of this study was to investigate the effect of treadmill exercise on catalepsy of Reserpine-induced Parkinson model in diabetic male rat.
Materials and Methods: In this study, Wistar rats (n=48) were divided into 8 groups (Control, Diabetes, Exercise, Parkinson, Parkinson+Diabetes, Parkinson+Diabetes+ Exercise, Parkinson+Exercise and Diabetes+Exercise). Diabetes was induced by single injection of Streptozotocin (60 mg/kg, i.p) and Parkinson's model was induced by the injection of Reserpine (1mg/kg, i.p) for 2 days. The bar-test was used to measure cataleptic symptoms. All animals in the Exercise group were forced to run on a motorized treadmill (24 m/min for 20 min, 5 day/week) for 3 weeks. The SPSS software was used for analyzing data using the ANOVA at a significance level of P<0.05.
Results: The catalepsy was significantly more and less sever in the Diabetes + Parkinsonm and Parkinson+ Diabetes+Exercise group than the Diabetes+Parkinson group, respectively.
Conclusion: While diabetes can influence the Reserpine-induced catalepsy in animal Parkinson model, exercise can improve it.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
The effect of edible oil from Portulaca oleracea seeds on the blood-brain barrier permeability in rat
405
412
FA
Marzyeh
Varnaseri
Department of Physiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, I. R. Iran.
Mahdi
Rahnema
Department of Physiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, I. R. Iran.
Mohammad Reza
Bigdeli
Department of Physiology, Shahid-Beheshti University, Tehran, I. R. Iran.
Background: After cancer, cardiovascular and respiratory disorders, stroke is considered as the four major death reasons in general population. Due to the important role of Portulaca oleracea in reducing the inflammatory damages and its protective effect on hypoxic nervous tissue, the present study was conducted to investigate the relationship between the intake of Portulaca oleracea oil and the blood-brain barrier (BBB) permeability in a rat stroke model.
Materials and Methods: In this experimental study, male Wistar rats (n=35) were randomly allocated into 5 equal groups: Control (distilled water+ischemia); three Experimental groups (Portulaca Oil at doses of 50, 75 and 100 mg/kg+ischemia) and Sham group (no treatment and ischemia). Pretreatment with Portulaca Oil was performed for 30 days, orally through gavage. In each of the groups blood-brain barrier permeability was studied using the Evans-Blue concentration.
Results: Compared to the Control, the permeability of BBB was decreased only in Experimental groups with 0.50 and 0.75 mg/kg doses. However, the Experimental group with a dose of 0.25 did not reveal any significant difference compared to Control.
Conclusion: Based on the results of this study, as a precautionary measure for stroke the administration of Portulaca oleracea oil can reduce the BBB permeability.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
The effect of alcoholic extract of Stachys lavandulifolia Vahl leave on alpha motoneuron regeneration of anterior horn in a rat model of sciatic nerve compression
413
419
FA
Maryam
Tehranipour
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran.
Fatemeh
Attarian
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran.
Background: Peripheral nerve injury during accidents causes the degeneration of the cell body in anterior horn of the spinal cord. Sometimes the use of plant extracts may be effective in regenerative process of these injuries. Stachys lavandulifolia of the Laminacea family has antioxidant and anti-apoptotic effects. The aim of this study was to investigate the regenerative effect of alcoholic extract of Stachys lavandulifolia leave on spinal cord alpha motoneuron in a rat model of sciatic nerve compression.
Materials and Methods: In this experimental study male Wistar rats (n=36) were divided randomly into six groups: Control, Compression, and Compression + Treatment (25, 50, 75, 100 mg/kg of extract). In order to induce compression, sciatic nerve of right leg was exposed to compression for 60 seconds using the locker pincers. Extract injection was done intraperitoneally in the first and second weeks after the compression. Then, 28 days after the compression and under profusion method, the samples were taken from lumbar spinal cord. The density of motoneurons was measured and compared using the dissector and stereological methods.
Results: According to the present results, the density of neurons in Compression group was decreased significantly compared to the Control (P<0.001) one. Moreover, Neuronal density was increased significantly in all Treatment groups (25, 50, 75, and 100 mg/kg doses) compared to the Compression group (P<0.01).
Conclusion: The alcoholic extract of Stachys lavandulifolia leave has neuro-protective effect on anterior horn neurons of the spinal cord after injury. Probably, this effect is related to the existence of antioxidant and anti-inflammatory factors in alcoholic extract that induces the neuronal regeneration.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Reverse staining: Effective method for purification of HIV-1 Nef protein in prokaryotic expression system
420
426
FA
Behnaz Sadat
Jafarzade
Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, I. R. Iran.
Seyyed Mehdi
Sadat
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, I. R. Iran.
Ramin
Yaghobi
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, I. R. Iran.
Azam
Bolhassani
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, I. R. Iran.
Background: Nef protein is one of the HIV regulatory proteins. This protein has various conserved epitopes inducing the efficient immune responses in HIV-1 infected individuals. Thus, Nef protein has been proposed as a suitable candidate for vaccine design. In current study, our goal was the cloning and expression of Nef protein in prokaryotic expression system and its purification using the reverse staining method.
Materials and Methods: The coding sequence of Nef protein was amplified from pUC19-nef vector by PCR. Then, nef gene was inserted into the pGEX6p2 expression vector. This construct was transformed into the E.coli BL21 E.coli strain and subsequently protein expression was induced by IPTG anti-repressor. The protein expression was confirmed by SDS-PAGE and Western blot using anti-Nef antibody. Protein purification was performed by reverse staining method.
Results: The PCR and digestion analysis showed a clear band of 648 bp in agarose gel indicating the correct cloning of HIV-nef in pGEX6p2 expression vector. In addition, the detection of a clear 50 kDa band in Western blotting using Anti-Nef antibody suggests the Nef protein expression induced by IPTG. Finally, the purified protein was obtained by reverse staining method.
Conclusion: The recombinant Nef protein expressed in E.coli was purified by reverse staining method. The Nef protein has the potential of antigenicity for vaccine designing against HIV infections.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Effects of aerobic and exhaustive exercise on salivary and serum total antioxidant capacity and lipid peroxidation indicators in sedentary men
427
434
FA
Vahid
Sari-Sarraf
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, I. R. Iran.
Ramin
Amirsasan
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, I. R. Iran.
Hamid Reza
Zolfi
Ph.D Candidate, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, I. R. Iran.
Background: Some studies have shown that aerobic exercise could promote the antioxidant defense system. Therefore, the aim of present study was to investigate the effects of aerobic and single bout of exhaustive exercise on salivary and serum malondialdehyde (MDA) and total antioxidant capacity (TAC) in non-athlete males.
Materials and Methods: Twenty-seven male (age: 18-21 year) were randomly assigned into Control (n=12) and Aerobic-training (n=15) groups. Training group was participated in an aerobic exercise program (50-70% of Heart Rate Reserve) for 8 weeks (three times per week). After the termination of exercise program, both groups performed a progressive exercise to exhaustion on treadmill. To evaluate the MDA and TAC, serum and salivary samples were collected before and after the training as well as after the exhaustive exercise.
Results: Aerobic exercise caused a significant increase in salivary and serum TAC (P<0.05). Moreover, the intensive exercise reduced the serum TAC in Control group (P<0.05). After the termination of exercise program, serum MDA was lower in Training group than the Control one, while no difference was noted for salivary measurement. A significant rise in serum MDA was found in Control group after the exhaustive exercise (P<0.05). However, there was no significant relationship between the salivary and serum TAC and MDA levels.
Conclusion: Aerobic exercise could be effective in preventing the exhaustive exercise-induced lipid peroxidation. Although the interpretation of results on salivary parameters require further studies.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
The prevalence of Set1 A and Set1 B genes in clinical Shigella sonnei strains using multiplex-PCR
435
440
FA
Mina
Sadeghifard
Department of Microbiology, Faculty of Basic Science, Saveh Branch, Islamic Azad University, Saveh, I. R. Iran.
Kumarss
Amini
Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh I. R. Iran.
Javad
Nasr
Department of Microbiology and Immunology, Faculty of Veterinary medicine, University of Tehran, Tehran, I. R. Iran.
Ramak
Yahyaraeyat
-
Background: Shigella spp have been reported as the common cause of mortality in children. Recent studies in Iran have shown that Shigella sonnei is an important specie for nosocomial infection. The set1A and set1B are two critical virulence factors for human disease. The aim of this study was to evaluate the frequency of set1A and set1B genes in bacterial strains isolated from people with diarrhea dysentery using the multiplex-PCR (M-PCR) method.
Materials and Methods: A total 60 strains of Shigella sonnei were collected from Children Medical Center (Tehran, Iran). Then, these isolates were identified by standard biochemical and culture phenotypic methods. Detection of both of set1B and set1A virulence genes was carried out by M-PCR. Antibacterial susceptibility testing to Amoxicillin, Clavulanic acid, Tetracycline, Cefixime, Ceftriaxone, Cefepime and Cotrimoxazole was performed according to CLSI guideline using disk diffusion method.
Results: All 60 isolates were identified as S. sonnei. The highest resistance was observed to both of Cotrimoxazole and Cefixime antibiotics, while 95% of strains were sensitive to Tetracycline. The prevalence of Set1A and set1B virulence genes were 18 (30%) and 0 (0%) strains, respectively.
Conclusion: Nowadays, the increase of microbial resistance to antibiotics is a global problem which is caused by indiscriminate use of drugs. The results showed that the prevalence of the gene in the sample set could be a criterion for direct detection of Shigella in the sample.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Association of miR-143 rs41291957 and rs4705342 genetic variants with endometriosis risk in infertile women
441
446
FA
Elaheh
Nimi-Hoveidi
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran.
Leila
Kohan
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran.
Seyyedeh Sara
Hashemi
Department of Stem Cell, Mother and Child Ghadir Hospital, Shiraz University of Medical Science, Shiraz, I. R. Iran.
Background: Infertility is one of the major human problems. One of the most important causes of infertility is endometriosis. Endometriosis, defined as the growth of endometrial tissue outside the uterine cavity, is a common gynecological disorder. Recent research has shown that miRNAs and their target mRNAs are differentially expressed in endometriosis. The aim of this study was to investigate the association of miR-143 rs41291957 and rs4705342 gene variants with endometriosis in infertile women.
Materials and Methods: This case-control study was done on Iranian women (n=303) from March to February 2014. Blood samples from infertile women (n=77) with endometriosis and age-matched healthy women (n=226) at the Zeinabieh Hospital (Shiraz, Iran) were collected. The MiR-143 rs4705342 and rs41291657 genotypes were determined using Tetra-ARMS PCR and PCR-RFLP methods, respectively.
Results: There was a significant difference in the genotype distribution and allele frequency of rs4705342 polymorphism between case and control groups; TC genotype (OR: 2.3, 95%CI: 1-5.3, P=0.04) and C allele were associated with an increased risk of endometriosis (OR: 2.24, 95% CI: 1-4.9, P=0.04). Also, an allele of rs41291957 polymorphism showed a significant association with susceptibility to endometriosis (OR: 2, 95% CI: 1-4, P=0.04).
Conclusion: The finding of this study indicated that for the first time, there was a significant association between miR-143 polymorphisms and the risk of endometriosis. Further studies in other populations with larger samples are needed to confirm these findings.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Design, transformation and proliferation of VEGF111b recombinant isoform in Escherichia coli Top10 in order to produce recombinant drugs
447
453
FA
Morteza
Sadegi
Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, I. R. Iran.
Zohreh
Hojjati
Department of Biology, Faculty of Science, Isfahan University, Isfahan, I. R. Iran.
Background: The synthesis of recombinant proteins with the aim of inhibiting the tumor receptors is one of the new approaches in cancer treatment. The aim of this study was to design and clone the VEGF 111b recombinant isoform in pBudCE4.1 vector and assess its compatibility with E.coli Top10 in order to produce some recombinant drugs.
Materials and Methods: The VEGF111b new isoform designed using gene sequences available in the databases and oligo 7 software was digested by BglII and KpnI enzymes. Then it was cloned at downstream of the EF-1 promoter in the pBudCE4.1vector.
Isolation of recombinant bacteria was done in LBA medium with the concentration of Zeocin antibiotic (3% and 5%). In the final step, the recombinant vector was extracted using DNA gel extraction kit and VEGF111b recombinant fragment was confirmed by enzyme digestion and sequencing.
Results: The ligation of 111b fragment in expected site was confirmed. The entire E.coli Top10 colonies were observed in Zeocin medium (5%) containing recombinant VEGF111b fragment and recombinant colonies (61.9%) were observed at Zeocin medium (3.0%). The existence of VEGF111b sequences in recombinant bacteria was confirmed by enzyme digestion and sequencing.
Conclusion: The present study is an important step in the production process of VEGF111b recombinant protein and evaluating its anticancer effect. Moreover, the pBudCE4.1 vector containing 2 cloning sites and 8 enzyme excision sites is a good candidate for cloning and expression of recombinant proteins in E.coli TOP10.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
The presence of Helicobacter pylori in gallbladder mucosa of patients with chronic cholecystitis
454
460
FA
Farzad
Kheirmand
School of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, I. R. Iran.
Fahimeh
Valadi
School of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, I. R. Iran.
Kamran
Dolatshahi
School of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, I. R. Iran.
Background: Helicobacter pylori (H. pylori) has infected over half of the world’s population. Among the long-term outcomes of H.pylori infection is gastric malignancies, particularly gastric cancer and gastric mucosa-associated tissue (MALT) lymphoma. In recent years, the attention has been drawn to the possible association of H. pylori infections not only with gastric and duodenal disorders but also with several gastrointestinal diseases (e.g. liver and biliary diseases). H. pylori have recently found to be associated with some diseases of the biliary tree. Earlier reports on the detection of H. pylori in the gallbladder mucosa of patients with biliary disease have shown contradictory results which requires further studies. The aim of this cross-sectional study was to determine the presence of H. pylori in gallbladder mucosa of patients with a diagnosis of chronic cholecystitis using histological study of surgical gallbladder specimens.
Materials and Methods: In the present study, gallbladder samples (total no=70) from patients with chronic cholecystitis were collected at Shariati hospital (Isfahan, Iran) from February to April 2015. Giemsa staining was used to detect H. pylori in gallbladder mucosa.
Results: Using Giemsa staining in 18 (25.7%) out of 70 patients gallbladder mucosa were positive for H. pylori. There was an association between the presence of H. pylori in the gallbladder mucosa and chronic cholecystitis. (P<0.001)
Conclusion: H. pylori can be detected in gallbladder mucosa of patients with chronic cholecystitis. Further studies are needed to support these findings.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Prevalence of class I, II and III integrons in the antibiotic-resistant isolates of A. baumannii detected from patients hospitalized in medical centers of Shahrekord
461
468
FA
Fahimeh
Nourbakhsh
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran.
Vajiheh
Nourbakhsh
Hazrat Fatemeh Zahra (SA) Hospital, Depending on the Treatment and Management of Social Security in Isfahan, Isfahan, I. R. Iran.
Mohammad Taghi
Jafakesh
Hazrat Fatemeh Zahra (SA) Hospital, Depending on the Treatment and Management of Social Security in Isfahan, Isfahan, I. R. Iran.
Background: Acinetobacter baumannii is an opportunistic and gram-negative coccobacillus. This opportunistic pathogen infectivity, especially in the intensive care units of hospitals is extensive worldwide. Due to the incremental pattern of antibiotic resistance, this study was carried out to track the Class I, II and III integrons in Acinetobacter baumannii isolated from hospitalized cases in medical centers (Shahrekord, Iran).
Materials and Methods: In this cross-sectional study A. baumannii isolates (n=100) detected from hospitalized patients in various wards of hospitals and health centers of Shahrekord in first six months of 2015 and antibiotic resistance pattern were determined by disk diffusion method. The presence of genes coding class I, II and III integrons were investigated using M-PCR method.
Results: Among the 100 A. baumannii studied isolates, the highest and the least resistance was seen for the of Cefepime (89%), Ciprofloxacin (95.4%) Ceftazidime (91.3%); and Chloramphenicol (3.7%) and Nitrofurantoin (2.9%) antibiotics, respectively. The frequency of Class I, II and III integrons was 100%, 44% and 3%, respectively.
Conclusions: The high incidence of integron classes among A. baumannii strains reflects the special roles of integrons in the acquisition pattern of multiple antibiotic resistance from isolates. Thus, providing the precautionary measures to prevent arbitrary use of antibiotics is suggested.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Correlation between emotional intelligence and accident among the motorcycle riders in Kashan
469
476
FA
Fatemeh
Asgarian
Trauma Research Center, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Negin
Masoudi-Alavi
Trauma Nursing Research Center, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Mohammad
Aghajani
Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Alireza
Ahmadi
Student Research Committee, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Background: Emotional Intelligence (EI) is considered as a new topic in the field of health. Studies conducted in this field have explained the role of EI in various aspects of life (e.g social relations). The present study was carried out to assess the correlation between the emotional intelligence and accident among the motorcycle riders in Kashan (Isfahan, Iran).
Materials and Methods: The sampling was done using the convenience method from the motorcycle riders referred to Beheshti Hospital in Kashan during 2014. Data were collected using two EI (demographic and Bar–on) questionnaires. Data were analyzed using Mann-Whitney U, Chi-square, independent t and the logistic regression for assessing the relationship between the emotional intelligence components and other variables with accident.
Results: The Mean EI and 9 subscales from the total 15 scales were significant between two groups. There was a significant difference between motorcycle riding with and without an accident in the Mean overall EI, so that the Mean was higher in motorcycle riders without an accident (P<0.05).
Conclusion: Our findings confirmed the important role of EI in extending the skills of emotional intelligence in the prevention of accidents and the importance of early prevention among the high risk groups. Moreover, the importance of the underlying educational programming for improving EI in such groups is considered a necessity.
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
20
5
2016
11
1
Brucella spondylodiskitis: Review of five cases
477
482
FA
Reza
Razzaghi
Department of Infectious Diseases, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Mansooreh
Momen-Heravi
Social Determinants of Health (SDH) Research Center, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Abolfazl
Zahedi
Student Research Committee, Kashan University of Medical Sciences, Kashan, I. R. Iran.
Mohsen
Ghorbiani
Veterinary Medicine Student, Shabestar Branch, Islamic Azad University- Young Researchers Club-Shabestar, I. R. Iran.
Background: Brucellosis, as an endemic disease in Iran, has bone and joint complications. The musculoskeletal involvement is among the most common complication of the disease. Here we review 5 cases of brucella spondylodiskitis.
Case Presentation: Three out of 5 cases were male and 2 remaining female. The Mean age of patients was 58.8±16.3 years. The most common clinical symptom was low back pain. There was a familial history of brucellosis in one case. Three cases had previous history of brucellosis. Three cases had consumed unpasteurized diary. All cases were positive for agglutination tests and all 5 cases had lumbar vertebral involvement in their MRI. Para-vertebral abscess were developed in 2 cases. All cases were cured with long-term medical treatment.
Conclusion: Regarding to high prevalence of brucellosis and the high frequency of its skeletal involvement, brucella spondylodiskitis should be considered among the differential diagnosis of low back pain. The early diagnosis and treatment of the disease could prevent the paravertebral abscess and other life-threatening nervous complications.