Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
The effect of lithium carbonate on the hypothalamic-pituitary-gonadal axis in adult female Wistar rats
450
456
FA
Seyyed Ebrahim
Hosseini
Shiraz Branch, Islamic Azad University
ebrahim.hossini@yahoo.com
Y
Zahra
Dalaeli
N
Background: Lithium is one of the elements of the alkali metal family used in pharmaceutical formulations, which can be used for the treatment of mental disorders, hypothyroidism and depression. This study aimed to determine the chronic effects of lithium on the estrogen, progesterone, FSH, LH hormones and ovarian follicles in adult female Wistar rats.
Materials and Methods: In this experimental study, 40 adult female rats were divided into five groups of 8 rats, including control, sham and three experimental groups receiving intraperitoneal doses of 60, 120 and 180 mg/kg lithium carbonate for 21 days, respectively. At the end of the third week, and after taking the blood samples to measure estrogen, progesterone, FSH and LH hormones, the rats;#39 ovaries were removed and after the preparation and staining of the tissue sections, the follicles were counted.
Results: The results showed that lithium significantly caused to increase the number of atresia follicles and also decrease primordial, primary, and secondary follicles, graphs, corpus luteum and estrogen, progesterone, FSH and LH hormones (P≤0.05).
Conclusion: According to the results of the current study, the use of lithium can increase atresia follicles and decrease other follicles and corpus luteum in adult female rats, and probably due to the reduction of follicles and corpus luteum, the sexual hormone levels are also decreased.
Lithium, Ovaries follicles, Estrogen, Progesterone, FSH, LH
http://feyz.kaums.ac.ir/article-1-2909-en.html
http://feyz.kaums.ac.ir/article-1-2909-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Apoptotic effects of silver nanoparticles coated with Zataria multiflora leaves extract on HepG2 cell line
457
467
FA
Negar
Khorasani
N
Javad
Baharara
Mashhad Branch, Islamic Azad University
baharara78@gmail.com
Y
Alireza
Iranbakhsh
N
Tayebeh
Ramezani
N
Background: Silver nanoparticles (AgNPs) have antibacterial, anti-angiogenesis and anti-cancer activity. The purpose of this study was to examine the apoptotic effects of AgNPs coated with Zataria multiflora leaves extract on HepG2 liver cancer cells.
Materials and Methods: HepG2 cancer cell lines were treated with different concentrations of AgNPs for 24 and 48 hours. The viability of the cells and inhibitory concentration (IC50) were determined by the methylthiazol tetrazolium (MTT) assay. To evaluate the ability of AgNPs to induce apoptosis in HepG2 cells, the 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI), acridine orange-propidium iodide staining and flow cytometry analysis for annexin V- propidium iodide were used.
Results: The results of the MTT assay showed that AgNPs decreased the proliferation of HepG2 cells in dose- and time-dependent manner. The IC50 during the incubation times of 24 and 48 hours were determined 40 and 30 μg/mL, respectively (P<0.05). The results of DAPI staining showed that AgNPs can lead to break down of nuclear DNA. Moreover, the acridine orange-propidium iodide staining and annexin V-propidium iodide showed that the percentage of apoptotic cells was increased in the treated cells.
Conclusion: Silver nanoparticles covered with Z. multiflora leaf extract have the ability to induce apoptosis in cancer cells HepG2 and they can be considered as a promising strategy for the treatment of liver cancer.
Silver nanoparticles, Cancer, Apoptosis, Zataria multiflora
http://feyz.kaums.ac.ir/article-1-2910-en.html
http://feyz.kaums.ac.ir/article-1-2910-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Synergistic effect of application of direct current electricity in combination with selenium nanoparticles on the killing of Leishmania major promastigotes in vitro
468
475
FA
Farnosh
Jamei
N
Abdolhosein
Dalimi–Asl
Tarbiat Modares University
dalimi_a@modares.ac.ir
Y
Mehdi
Karimi
N
Abbas
Dalimi
N
Fatemeh
Ghaffarifar
N
Background: Regarding some reports on the resistance of Leishmania to Glucantime and the absence of an effective vaccine for the prevention of cutaneous leishmaniasis, there is an urgent need to identify a new and effective therapeutic method. Therefore, the present study aimed to examine the effect of direct current electricity in combination with selenium nanoparticles on the killing of Leishmania major in vitro.
Materials and Methods: In this experimental study, the effect of different concentrations of selenium nanoparticles was evaluated against Leishmania major promastigotes in vitro. In the second step, the killing effect of selenium nanoparticles alone or in combination with 3 mA direct electric current was assessed in promastigote culture for 10 minutes. Then, the survival rate of the infected promastigotes was evaluated using the Microculture Tetrazolium Assay.
Results: The various concentrations of selenium nanoparticles significantly decreased the number of live promastigotes over time. Based on the parasite count, the half inhibitory concentration (IC50) of the nanoparticles was calculated 12.5 μg/ml after 24 hours of cultivation. Using selenium nanoparticles alone at the concentration of 100 μl/ml and in combination with 3 mA direct current electricity after 10 minutes caused 37.1% and 91.5% mortality in the promastigotes, respectively.
Conclusion: Although the use of selenium nanoparticles alone cannot kill the Leishmania major promastigotes completely, the combined use of both direct current electricity and selenium nanoparticles has a significant synergistic effect on promastigote mortality.
Leishmania major, Selenium nanoparticles, Direct current electricity, in vitro
http://feyz.kaums.ac.ir/article-1-2913-en.html
http://feyz.kaums.ac.ir/article-1-2913-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Role of NMDA glutamate receptors within the amygdale in inhibition of the metabolic effects of acute stress in male mice
476
485
FA
Bahareh
Sadeghi
N
Homeira
Zardooz
N
Hedayat
Sahraei
N
Nahid
Sarahian
Baqiyatallah University of Medical Sciences
sarahiannahid@yahoo.com
Y
Background: Amygdala is known as one of the most important regions of the brain in response to stressful stimuli. In the present study, the role of N-methyl-D-aspartate (NMDA) glutamate receptors within the amygdala in inhibition of the metabolic effects of acute stress in male mice was investigated.
Materials and Methods: In this experimental study, bilateral or unilateral amygdala cannulation was performed stereotaxically. After a seven-day recovery period, the animals (11 groups of seven each) received different doses of memantine (1, 0.5, and 0.1 µg/mouse) five min before the stress induction. Food and water intake, delay to eating time, and fecal material as stress metabolic parameters were measured.
Results: Stress had no effect on water intake, but reduced food intake and, increased delay to eating time and fecal materials. Moreover, injection of memantine to the right or left side of amygdala decreased water intake, and injection at a dose of 0.1 µg to the left amygdala inhibited the effect of stress and increased the food intake. Also, an injection of memantine in the left or right amygdala decreased delay to eating time and decreased fecal material at 1 and 0.5 µg/mouse doses that the right of the core had the greatest inhibitory effect in this regard.
Conclusion: It seems that the amygdala glutamate system, in particular its NMDA receptors, may have a significant effect on regulating the stress responses, which this effect is a side-dependent phenomenon.
Acute stress, Amygdala, Male mouse, NMDA glutamate receptor antagonist
http://feyz.kaums.ac.ir/article-1-2915-en.html
http://feyz.kaums.ac.ir/article-1-2915-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Comparison of the antibacterial effects of Persica, Matrica and chlorhexidine gluconate mouthwashes and normal saline in mechanically ventilated ICU patients: A clinical trial
486
494
FA
Abolfazl
Firouzian
N
Hadi
Darvishi-Khezri
Sari Branch, Islamic Azad University
Hadidarvishi@iausari.ac.ir
Y
Background: Bacterial colonization in the pharynx is one of the most important risk factors for ventilator-associated pneumonia. The aim of this study was to determine and compare the anti-bacterial effects of different mouthwashes in mechanically ventilated patients.
Materials and Methods: In this double-blind randomized clinical trial, 80 patients were selected from the intensive care units (ICUs) of Imam Khomeini Hospital in Sari City, Iran, during 2010-2011, and were divided into four groups of 20 cases. The first intervention group was administered with chlorhexidine gluconate (CHX) 0.2% mouthwash, the second group Persica herbal 10% mouthwash, the third group was administered with Matrica 10% and finally in the fourth group, normal saline was used. In order to culture Staphylococcus aureus and Streptococcus pneumoniae, oropharynx samples were obtained without any stimulation prior and 6 min following oral rinsing.
Results: Bacterial colonies were decreased significantly after the intervention in all four groups (P<0.001). Chlorhexidine gluconate, Persica and Matrica mouthwashes caused to decrease the Staphylococcus aureus (P<0.001, P<0.008 and P=0.01, respectively) and Streptococcus pneumoniae (P<0.001) positive cultures after the intervention.
Conclusion: Herbal oral mouthwashes including Persica and Matrica are effective in decreasing Staphylococcus aureus and Streptococcus pneumoniae colonized in the oropharynx of mechanically ventilated patients. Therefore, after further investigations, they can serve as suitable alternatives for CHX in ICU settings.
Mouthwashes, Chlorhexidine, Persica, Matrica, Normal Saline, Intensive Care Unit, Mechanical ventilation
http://feyz.kaums.ac.ir/article-1-2916-en.html
http://feyz.kaums.ac.ir/article-1-2916-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
The effect of aerobic training on the serum level of adipolin and insulin resistance in overweight men
495
503
FA
Rahman
Soori
University of Tehran
Soorirahman@yahoo.com
Y
Mohammadreza
Asad
N
Zohreh
Barahouei-Jamar
N
Najmeh
Rezaeian
N
Background: Adipolin (CTRP12) is a novel anti-inflammatory adipocytokine secreted from adipose tissue, which improves insulin sensitivity. The aim of this study was to examine the effect of aerobic training on serum adipolin levels and insulin resistance index (HOMA-IR) in overweight men.
Materials and Methods: In this semi experimental study, which was conducted in one of the sport complexes of Khorramabad in spring 2014, 26 sedentary overweight men (body mass index (BMI)>25 Kg/m2, age range 35-50 years) were selected and randomly allocated into the experimental (n=13) and control (n=13) groups. Subjects in the experimental group participated in 10 weeks of aerobic training at 50%-70% of the maximal heart rate, 30-45 minutes per session and three sessions per week. Levels of adipolin, insulin and fasting glucose and anthropometric indices were measured before and 72 hours after the last training session.
Results: Serum levels of adipolin, insulin, fasting glucose and HOMA-IR did not significantly change following 10 weeks of aerobic training (P>0.05). However, weight (P=0.001), BMI (P=0.001) and fat percentage (P=0.001) were decreased significantly. Furthermore, changes in adipolin levels following 10 weeks of aerobic training were significantly correlated with changes in BMI (P=0.028, r=0.606).
Conclusion: A 10-week aerobic training program has no effects on serum levels of adipolin in sedentary overweight men and these findings are in accordance to insulin resistance changes.
Aerobic training, Adipolin, Insulin resistance, Overweight men
http://feyz.kaums.ac.ir/article-1-2917-en.html
http://feyz.kaums.ac.ir/article-1-2917-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Investigating the predictive role of working memory in risky decision-making in children with attention deficit and hyperactivity disorder
504
510
FA
Maryam
Najarzadegan
N
Vahid
Nejati
Shahid-Beheshti University
nejati@sbu.ac.ir
Y
Nasrin
Amiri
N
Background: Risky decision-making can be considered as one of the main deficits in attention deficit and hyperactivity disorder (ADHD). There are various factors that lead to decision-making deficit in children with ADHD, including deficit in working memory as a component of executive functions. This study aimed to examine the relationship between working memory and risky decision-making in children with ADHD.
Materials and Methods: In this cross-sectional study, 45 children (19 girls and 26 boys) with ADHD were selected using the non-probability sampling method. Children with comorbid disorders were excluded from the study. The N-Back test and the balloon analogue risk task (BARAT) were used to evaluate working memory and risky decision-making, respectively.
Results: The results of Pearson correlation showed that working memory was correlated with risky decision-making (P<0.01). Moreover, the results of step-wise regressions showed the predictive role of working memory in risky decision-making of the participants (P<0.01).
Conclusion: Results of this study show that working memory is negatively correlated with risky decision-making. Also, working memory scores can predict risky decision-making in children with ADHD. Therefore, it can be concluded that risk-taking will be reduced by improving working memory.
Working memory, Executive function, Attention deficit and hyperactivity disorder, Decision-making
http://feyz.kaums.ac.ir/article-1-2919-en.html
http://feyz.kaums.ac.ir/article-1-2919-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
The effect of hydroalcoholic extract of Satureja hortensis on outcomes of stroke in rats
511
519
FA
Mehdi
Rahnema
Zanjan-Branch, Islamic Azad University
meh_rahnema@yahoo.com
Y
Meysam
Foroozandeh
N
Elham
Ghasemloo
N
Background: Antioxidants can prevent oxidative stress produced by free radicals. Since Satureja hortensis contains various antioxidant compounds, this study was conducted to examine the effect of S. hortensis extract on blood-brain barrier permeability, brain edema and neurological deficits.
Materials and Methods: In this experimental study, 70 male Wistar rats were randomly allocated into 5 groups (n=14). The control group received distilled water plus induction of ischemia, three experimental groups received the hydroalcoholic extract of S. hortensis with different doses of 50, 75 and 100mg/kg, respectively, plus induction of ischemia and the sham group received no treatment and induction of ischemia. Pretreatment with S. hortensis extract was performed for 30 days, orally through gavage. Each group was subdivided into two subgroups (n=7) in order to assess the blood-brain barrier permeability (concentration of Evans Blue) and brain edema (brain water content). Moreover, neurological deficit scores were evaluated in the two mentioned subgroups.
Results: Results of the current study showed that S. hortensis extract reduced the concentrations of Evans Blue (8.93±0.75, 7.69±0.74, 6.68±0.56, respectively) and brain water content (82.21±0.71, 81.88±0.69, 80.50±0.89, respectively) in three groups received the extract compared to the control group (84.46±0.81), and reduced neurological deficit scores in the two groups received 75 and 100mg/kg doses of the extract (1.43±0.27 and 1±0.21, respectively) compared to the control group (3.36±0.32).
Conclusion: It seems that S. hortensis extract can exert the neuroprotective effect against stroke damage by increasing the strength of blood-brain barrier and preventing brain edema.
Satureja hortensis, Stroke, Blood-brain barrier permeability, Brain edema, Neurological deficits
http://feyz.kaums.ac.ir/article-1-2920-en.html
http://feyz.kaums.ac.ir/article-1-2920-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Determination of allelic polymorphism of codon F31I of STK15 gene in invasive ductal breast carcinoma
520
526
FA
Rahman
Golmohammadi
Sabzevar University of Medical Sciences
rahimgolmohammadi@yahoo.com
Y
Mohammad Javad
Namazi
N
Background: The carcinogenesis role of allelic polymorphism of codon F31I [T/A] of the serine/threonine kinase-15 (STK15) gene in geographic-dependent invasive ductal carcinoma is still controversial and worth to be studied. This study aimed to identify allelic polymorphisms of F31I codon in women with breast cancer compared to healthy controls in Sabzevar city, north-east Iran.
Materials and Methods: This descriptive analytical study was conducted on 200 women including 100 patients and 100 healthy controls. DNA samples were extracted using a standard kit and codon F31I was amplified by polymerase chain reaction (PCR). The polymorphisms of different genotypes were identified by restriction fragment length polymorphism (PCR-RFLP) analysis and electrophoresis.
Results: The frequency of heterozygote phenylalanine/isoleucine (Phe/Ile) was 70 (35%) in the cancerous cases and 82 (41%) in controls. The frequency of homozygote isoleucine/isoleucine (Ile/Ile) was 30 (15%) in the patients and 18 (9%) in controls. The results did not show homozygote phenylalanine/phenylalanine (Phe/Phe) in either patients or controls. Moreover, there was a significant higher homozygote Ile/Ile in the patients compared to controls (P<0.034).
Conclusion: For the first time, the study reports that there is a significant higher rate for homozygote Ile/Ile in cancerous patients compared to controls in Sabzevar city. Therefore, determination of allelic polymorphism of F31I codon of STK15 can be a clinically valuable test for diagnosis, prognosis and treatment purposes in breast cancer.
Breast cancer, Invasive ductal carcinoma, F31I codon, STK15 gene
http://feyz.kaums.ac.ir/article-1-2921-en.html
http://feyz.kaums.ac.ir/article-1-2921-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Comparison of serum homocysteine levels in patients with coronary artery disease with and without diabetes mellitus
527
532
FA
Hasan
Rajabi-Moghadam
N
Zahra
Chitsazian
Kashan University of Medical Sciences
z_chitsazian@yahoo.com
Y
Batool
Zamani
N
Maedeh
Memar
N
Background: Homocysteine is an amino acid that is produced during the metabolism of the methionine cycle. In previous studies, a causal role of homocysteine in coronary artery disease (CAD) has been investigated; however, the homocysteine level in diabetic and non-diabetic patients with CAD has not been compared. This study aimed to investigate this issue.
Materials and Methods: This cross-sectional study was conducted on 200 patients with CAD in Kashan Shahid Beheshti Hospital during 2014-2015. Coronary artery disease was confirmed by angiography. Homocysteine levels, blood urea nitrogen, creatinine, hemoglobin A1C and mean blood pressure were measured. Data were analyzed using t-test with SPSS version 16.
Results: In this study, one hundred and eight of patients (54%) were men. The mean concentrations of hemoglobin A1C in diabetic and non-diabetic patients were 6.69±1.44 and 5.74±0.92, respectively (P<0.001). Moreover, the mean homocysteine serum levels in diabetic and non-diabetic patients were 19.89±6.86 μmol/L and 24.35±9.93 μmol/L, respectively (P<0.001).
Conclusion: Results of the current study showed that the homocysteine serum level in patients with CAD was higher than the normal level and in patients without diabetes was significantly higher than patients with diabetes. Also, in diabetic patients with CAD, the serum creatinine and urine protein levels were higher than those in non-diabetics patients.
Diabetes, Coronary artery disease, Homocysteine
http://feyz.kaums.ac.ir/article-1-2922-en.html
http://feyz.kaums.ac.ir/article-1-2922-en.pdf
Kashan University of Medical Sciences
Feyz Medical Sciences Journal
1029-7855
2008-9821
19
6
2016
2
1
Psychometrics of the recreation experience preference scale in Iranian elderly
533
542
FA
Alireza
Farsi
N
Zahra
Fathirezaie
University of Tabriz
zahra.fathirezaie@gmail.com
Y
Seyyed Hojat
Zamani-Sani
N
Background: The researches have shown that the motivation to health is an important dimension in leisure satisfaction. The aim of this study was to measure the validity and reliability of the recreation experience preference scale (REP scale) in Iranian elderly.
Materials and Methods: A total of 552 elderly participants (347 males, 205 females) aged 60 years or older were randomly selected by the multistage sampling method from 9 provinces of Iran in 2013. Demographic characteristic questionnaire and recreation experience preference scale were used to collect data. Data were analyzed using an exploratory factor analysis with Varimax rotation, confirmatory factor analysis for construct validity, Pearson correlation for internal consistence construct validity, Cronbach;#39s coefficient alpha for reliability and multivariate analysis of variance for construct validity.
Results: Results showed that the REP scale had an acceptable factor structure. The matrix table of rotated elements showed five components were capable of factoring and 17 items predicted 71.06% of variance. Confirmatory factor analysis showed that the first- and second-order model at the scale of the study was approved appropriately. Also, the internal consistency of the scale in total and its subscales was acceptable (Cronbach;#39s alpha ranged from 0.70 to 0.92). Moreover, a positive and significant correlation between items and factors, also between factors and total scale showed that these questions assessed the same subject.
Conclusion: This study represents acceptable reliability and validity of the REP scale in Iranian elderly. Therefore, it can be used for measuring the REP and motivation in Iranian elderly.
Experience preference, Elderly, Leisure time, Validity, Reliability
http://feyz.kaums.ac.ir/article-1-2923-en.html
http://feyz.kaums.ac.ir/article-1-2923-en.pdf