@article{ author = {Edalatmanesh, Mohammad Amin and Sadoughi, Seyyed Damoo}, title = {Effect of aqueous extract of Ferula assa-foetida L. resin on angiogenesis in rat aortic ring model}, abstract ={Background: Besides embryogenesis, the angiogenesis is required for many physiological and pathological conditions (e.g. tumor growth). Various studies have shown the anti-proliferative and anti-cancer effects of Ferula assa-foetida (FF) resin. In the present study using a Wistar rat aortic model the effect of aqueous extract of FF resin on the angiogenesis has been investigated. Materials and Methods: In this experimental study, Wistar rat aorta was divided into strips (1 mm) and cultured in collagen matrixes. Following the observation of angiogenic sprouting on third day, the samples were classified into 4 groups (n=8 in each group): Control group, Sham-exposed group (treated with sterile distilled water) and Experimental groups 1 and 2 (treated with 100 and 200 μg/ml of aqueous extract of FF resin, respectively). After 24 hours, the angiogenesis was assessed using the invert microscopy followed by the photography of all samples. The length and number of blood vessels were measured using Image J software. Results: The Mean for length (143.8±5.3) and number (51.2±7.3) of vessels in Control samples showed no significant difference compared to Sham-exposed samples (138.1±3.8) and (46.3±5.4), respectively. The Mean length and number of vessels in the Experimental groups 1 (81.5±4.5; 26.8±3.5) and 2 (35.9±4.3; 7.7±2.6) were significantly decreased in a dose-dependent manner compared to the Control group (P<0.05). Conclusion: The aqueous extract of FF resin dose-dependently inhibits the angiogenesis. Therefore, the use of FF resin can be effective in inhibiting the angiogenesis.}, Keywords = {Angiogenesis, Ferula assa-foetida L., Aortic ring, Rat}, volume = {20}, Number = {2}, pages = {100-107}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3026-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3026-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Nazem, Farzad and Salehikia, Abbas and Marandi, Seyyed Mohammad and Sahdadi, Ahm}, title = {Impact of a 12 week resistance and concurrent training on bone mechanical strength and mineral density of osteoporotic male Wistar rats}, abstract ={Background: This study evaluated the efficacy of concurrent training compared to resistance training on femoral bone mineral density (BMD) and mechanical strength of osteoporotic male rats. Materials and Methods: Eight out of 40 male Wistar rats were separated as healthy subjects. Then osteoporosis was induced in the remaining rats by the injection of ethanol (20%, i.p) for 3 weeks. Osteoporotic rats divided into 4 groups: Baseline, Resistance training, Concurrent training and Control. The resistance training protocol consisted of 8 series of climbs on a 110-cm vertical ladder angled at 80º with weights tied to the animal tail. The overload for first and eighth series was set at 50% and 100% of the total body mass of the animal, respectively. Concurrent group completed a combination of both resistance and endurance treadmill trainings (60 min/day, speed: 12 m/min). The left excised femur of the animals were scanned for BMD measurement and examined by three point bending test to obtain the maximum force and stiffness. Result: Two Resistance training and Concurrent training groups had significantly increased maximum force, stiffness and BMD compared to Control group (P=0.019). Compared to resistance group, the Concurrent group showed a positive effect on bone mechanical strength (P=0.039) with no significance difference in BMD (P=0.890). Conclusion: The results of this study shows that the combination of resistance and endurance exercise may have a synergistic effect on increasing the mechanical strength of osteoporotic femoral bone in male rats compared to resistance training alone.}, Keywords = {Bone density, Mechanical properties, Osteoporosis, Resistance training, Concurrent training, Animal model}, volume = {20}, Number = {2}, pages = {108-117}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3040-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3040-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Abbassi-Daloii, Asieh and Abdi, Ahmad and Yazdani-Tapesari, Hoda and Salehpour, Mojtaba and Rostami-Angasi, Zahra and Yahyaei, Behroz}, title = {Effect of 8 weeks aerobic training on plasma apelin in male rats treated with L-NAME}, abstract ={Background: Evidence supports the systemic inflammation in obesity and insulin resistance. Apelin is secreted by adipose tissue and plays an important role in the inflammation process. The present study aimed to examine the effect of 8 weeks aerobic training on plasma Apelin in male rats treated with Nitro-L-Arginine-Methyl Ester (L-NAME). Materials and Methods: In this experimental study male Wistar rats (n=24, age 20 months) were randomly assigned into four groups: Control, Training, TrainingS+L-NAME and L-NAME. An aerobic training protocol was performed for eight weeks, 5 times a week with an intensity of 75-80% of maximal oxygen consumption. The rats were sacrificed 72 hours after the last exercise session and blood samples were collected. Data were analyzed by ANOVA and Tukey post hoc test at P<0.05 significancy level. Results: The results showed that plasma Apelin level did not change significantly after eight weeks of aerobic training compared to the Control group. However, the results showed that there was a significant difference in plasma Apelin between L-NAME and Control group (P<0.003). In addition, the results showed no significant difference between the insulin level and glucose in four groups. Conclusion: It seems that with increasing age plasma Apelin level is not affected by aerobic exercise. However, nitric oxide inhibitors can reduce the plasma Apelin level.}, Keywords = {Aerobic training, L-NAME, Plasma apelin, Rat }, volume = {20}, Number = {2}, pages = {118-124}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3027-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3027-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Saghravanian, Seyyed Javad and Fereidoni, Masoud and Asadollahi, Ali}, title = {Effect of hydroalcoholic extract of Ferula szowitsiana DC. on paw edema in rat}, abstract ={Background: Due to the side effects of chemical medicines, nowadays the use of drugs of natural origin is concerned. In traditional medicine plants of Ferula family generally regarded as the inhibitor of pain and inflammation. In this study using the sub-plantar formalin injection the anti-inflammatory effect of the hydroalcoholic extract of Ferula szowitsiana DC., as one of the species of this family, is investigated on rat paw edema. Materials and Methods: The hydroalcoholic extract of Ferula szowitsiana DC. was prepared and solved in Saline, Ethanol and Tween 80 (in a ratio of 8:1:1, respectively). Male Wistar rats (200-250 g) were assigned to: Control, Solvent (i.p), Solvent (i.t), Extract (50, 100, 200, 400 mg/kg, i.p), Naloxone (2 mg/kg, i.p), Naloxone-Extract (2 mg/kg, i.p and 400 mg/kg, i.p, respectively) and Extract (8 μg/10 μl, i.t) groups. For inducing inflammation Formalin 2.5% (0.05 ml) and for assessing the volume of edema plethysmometeric methods were used. Results: The extract of Ferula szowitsiana DC. reduced inflammation in a dose dependent manner (P<0.001). The effects of central (i.t.) administration of the equivalent concentration of the effective dose of extract were similar to their systemic administration. Naloxone partially reduced the anti-inflammatory effect of extract (P<0.05). Conclusion: The anti-inflammatory property of Ferula szowitsiana DC. extract is probably induced because of its effect on central nervous system (CNS) and part of this effect is mediated by opioid system.}, Keywords = {Ferula szowitsiana DC., Inflammation, Plethysmometer, Rat}, volume = {20}, Number = {2}, pages = {125-132}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3028-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3028-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Bashiri, Tahereh and Ghorbanian, Mohammad Taghi and Zavareh, Saee}, title = {Influence of alpha-lipoic acid on survival and proliferation of mesenchymal stem cells}, abstract ={Background: Mesenchymal stem cells (MSCs) contribute to tissue repair in vivo and form an attractive cell source for tissue engineering. The regenerative potential of MSCs is impaired by oxidative stress-induced cellular senescence. Alpha-lipoic acid (ALA) is well-known for its antioxidant properties. The Ki-67 antigen is expressed during all phases of cell cycle (G1, S, G2 and M phase) except for G0 phase and is commonly used as a proliferation marker. Herein, the aim of the present study was to investigate the impact of ALA on rat MSCs survival and proliferative potential in vitro. Materials and Methods: Isolated rat bone marrow and derived mesenchymal stem cells were synchronized by serum starvation for 24h and the addition of hydroxyurea (2µM). Afterwards, the cells were cultured in the presence of ALA (1µM) for 48h. An MTT assay was used to investigate cell survival and proliferation. The expression of Ki-67, a proliferation marker, was also evaluated. Results: The MMT assay showed a statistically significant increase in proliferation of MSCs in ALA-treated groups for 48 hours. Immunoctytochemistry of Ki-67 revealed significant differences between ALA- treated and Control groups. Conclusion: In conclusion, ALA is effective in increasing the survival and cell proliferation of isolated rat bone marrow and derived mesenchymal stem cells.}, Keywords = {Mesenchymal Stem Cells, Proliferation, Alpha Lipoic Acid, Ki-67 protein}, volume = {20}, Number = {2}, pages = {133-140}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3035-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3035-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Skandari, Marzieh and Nazemzadegan, Gholam Hosein and Daryanosh, Farhad and Samadi, Mehdi and Honarpisheh, Shirin and Hasanpor, Mahmou}, title = {Comparative effect of single bout of continuous endurance and high intensity interval exercise on serum BDNF in rat}, abstract ={Background: Continuous endurance exercise positively affects the brain via increasing brain-derived neurotrophic factor (BDNF). Therefore, in this study, comparative effect of single bout of continuous endurance exercise (CEE) and high intensity interval exercise (HIIE) on serum BDNF in rat was investigated. Materials and Methods: In this experimental study rats (n=30) have randomly divided into three (CEE, HIIE and Control) groups. Following habituation to treadmill all groups (Except for Control) performed the single bout exercise of the target group. At the end of the exercise blood samples were taken. Data were analyzed using ANOVA. Results: The results showed that BDNF level in the CEE group was significantly higher than HIIE (P<0.001) and Control group (P<0.001), while no difference between the Control and HIIE groups. Conclusion: It seems that CEE is considered as an appropriate intervention in increasing BDNF and this factor is more affected by the duration than the intensity of exercise. Therefore, for achieving benefits of exercise on the central nervous system, performing prolonged activities are probably more useful.}, Keywords = {BDNF, Continuous endurance exercise, High intensity interval exercise}, volume = {20}, Number = {2}, pages = {141-146}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3036-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3036-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Seyedalipour, Bagher and Hasani, Ali and Ebrahimzadeh, Mohammad Ali and Taravati, Ali}, title = {Antioxidant activity, total flavonoid and total phenolic contents of extracts taken from aerial parts of Ballota platyloma using three different methods: percolation, ultrasonic and polyphenolic fraction}, abstract ={Background: The genus Ballota L. (BL) belongs to the Labiateae family spread throughout the world.  Plants of this genus possess anti oxidant, anti cancer, anti microbial and anti inflammatory properties. This study aimed to investigate the antioxidant activities of extract taken from aerial parts of Ballota platyloma Rech. f. using percolation, ultrasonic and polyphenol fraction methods. Materials and Methods: In this experimental study, aerial parts of Ballota platyloma were collected from Veresk (Mazandaran, Iran). Dried aerial parts of Ballota platyloma were extracted by percolation, and polyphenol fraction. The antioxidant activity of extracts was investigated using different methods: 1, 1-diphenyl- 2-picryl hydrazyl (DPPH), ‏iron ion chelating activity and reducing power. Data were analyzed using ANOVA and Duncan test at a significant level of P<0.05. Results: The total phenolic and total flavonoid contents were in order of polyphenol> ultrasonic > percolation,  The IC50 for polyphenol, percolation and ultrasonic extracts were 7.52±1.07, 6.64±0.87 and 2.58±0.06, respectively. The IC50 for iron chelating ability were in order of polyphenol < ultrasonic < percolation. Polyphenol fraction showed better reducing power than the ultrasonic and percolation extracts (P<0.001) which was comparable to that of Vitamin C (P<0.05). Conclusion: The results showed that polyphenol fraction was rich in total phenolic and total flavonoid contents. Polyphenol fraction showed the best activity in reducing power but ultrasonic extracts was the most potent one in DPPH radical-scavenging activity. Thus, antioxidant activity may be attributed to the presence of phenols and flavonoids in the extracts.}, Keywords = {Antioxidant activity, Ballota platyloma, Flavoniods, Ultrasonic method, Polyphenol fraction}, volume = {20}, Number = {2}, pages = {147-156}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3039-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3039-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Moghaddam, Ali Asghar and Rahimi-Feyli, Peyman and Nikousefat, Zahra and Zarghami, Saj}, title = {Effects of different concentrations of sucrose and fetal bovine serum on viability rate of lamb spermatogonial stem cells before and after cryopreservation}, abstract ={Background: Spermatogonial stem cells (SSCs) have diverse applications in reproductive medicine and biotechnology. Cryopreservation is the well-known method for long-term storage of these cells. The aim of this study was to investigate the effect of different concentrations of fetal bovine serum (FBS) and Sucrose on the viability rate of spermatogonial cells. Materials and Methods: Testicular cells of pre-pubertal lambs were separated in a two-step enzymatic isolation and purified by differential plating. Then, the cells were divided in 6 groups. Groups 1, 2 and 3 were treated with FBS (10%) and Sucrose (0.07, 0.14 and 0.21 molar concentration (M) ) and groups 4, 5 and 6 with FBS (20%) and Sucrose (0.07, 0.14 and 0.21 M), respectively. The viability rate of the cells was evaluated immediately after isolation, addition of cryoprotectant agents and thawing procedures. Identification of spermatogonial cells in the culture was performed using the   immunocytochemistry staining against PGP9.5. Results: The results showed that cryoprotectant do not have any harmful effects on lamb´s SSCs. Moreover, viability rate of the cells in freezing media containing FBS (10%) is significantly higher than the media containing FBS (20%). Furthermore, increasing concentrations of Sucrose (0.07, 0.14 and 0.21 M)   had no beneficial effect on the spermatogonial viability rate. Conclusion: It was concluded that freezing media containing dimethyl sulfoxide (10%) and FBS (10%) and Sucrose (0.07 M) is appropriate for cryopreservation of lamb spermatogonial cells.}, Keywords = {Lamb Spermatogonia, Cryopreservation, Sucrose, Fetal bovine serum, PGP9.5}, volume = {20}, Number = {2}, pages = {157-164}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3041-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3041-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Monemiyan, Gita and Khoshkonesh, Abolghasem and Poorebrahim, Taghi}, title = {Effectiveness of solution- focused brief therapy on the reduction of couple burnout and optimizing the quality of marital relationship in married women}, abstract ={Background: Couple burnout, the main reason for low quality of marital relationship if untreated, will predispose the couples to divorce. In addition, it is one of the main problems that because of its adverse and destructive effects on marital relation can create psycho-emotional gap. This study aimed to address the effectiveness of solution-focused brief therapy in reducing marital burnout and optimizing the quality of marital relationship among the married women. Materials and Methods: This quasi-experimental study was performed with a pretest-posttest design and a purposeful, random assignment sampling. The data gathering tools was the couple Burnout Inventory of Pines (Couple Burnout Measure 1996), Johnson and Morrow (Inventory of Marital State 1988) which were completed by participants in the pretest period. The experimental group were exposed to the solution-focused brief therapy for eight sessions (one and half an hour each) with stepwise manner and a weekly sequence.  However, no intervention was performed in Control group. Following the completion of the sessions, the questionnaires were again completed by the participants. Results: The MANCOVA and ANCOVA analyses of data showed a significant reduction in marital burnout and optimization for quality of marital relationship among the married women participated in the study. Conclusion: The solution-focused brief therapy can be conducted by family counselors and therapists as an effective modality in reducing the marital burnout, optimizing the  quality of marital relationship for women and responding to their therapeutic needs.}, Keywords = {Solution- Focused Brief Therapy, Couple Burnout, Marital quality}, volume = {20}, Number = {2}, pages = {165-172}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3029-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3029-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Nozhat, Fatemeh and Zarei, M}, title = {Association between clinical symptoms and laboratory findings in the diagnosis of Candida vaginitis}, abstract ={Background: Vulvovaginal candidiasis is the second most common vaginal infection among women. In this study, the association between clinical symptoms and laboratory findings in diagnosis of Candida vaginitis has been investigated. Materials and Methods: This descriptive analytical study was carried out on women (n=220) with vaginitis symptoms. After diagnosing the Candida vaginitis based on the clinical symptoms (e.g. white color cheesy discharges, erythema or edema of the vulva and the vagina), two samples were taken from vaginal discharge for each participant. The first sample was cultured on Sabouraud dextrose agar medium in sterile conditions. Another sample was transferred directly to slide, on which a drop of the KOH (10٪) solution was added. The samples were immediately transferred to the laboratory for microscopic examination. The results were compared to clinical findings and analyzed by Chi-square and McNemar’s tests using SPSS software. P<0.05 was considered as the significant level. Results: One-hundred twenty four (56.4%), 64 (29.1%) and 78 (35.5%) out of 220 participants referred with vaginal complains were identified as vaginal candida infection in their physical examination, direct smear examination  and culture on Sabouraud dextrose agar, respectively. Conclusion: The mere apparent assessment of the clinical symptoms and patients complaints may be misleading the   diagnosis and treatment of the disease.}, Keywords = {Vulvovaginal Candidiasis, Candida albicans, Clinical symptoms, Female Genitalia }, volume = {20}, Number = {2}, pages = {173-179}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3030-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3030-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Ehsani, Majid and Atoof, Fatemeh and Nasri, Ne}, title = {Survival analysis of breast cancer cases under the regimens of anthracycline alone and combinaed with taxanes (2004-2012)}, abstract ={Background: Breast cancer (BC) is the most common cancer among women. Early diagnosis, treatment and prevention of BC is among the most debated topics in the world. Early diagnosis and appropriate treatment of BC can decrease the mortality rate to 2.2% per year. The aim of this study was to analyze the survival of patients with BC treated with anthracycline alone and combined to Taxane. Materials and Methods:  In this historic cohort study the BC cases (n=160) referred to oncology clinics during 2004-2012 were divided to two treatment groups (Anthracycline alone and Anthracycline+Taxane). Patients were followed-up until death or exclusion from    study. On demand, every 6 months a bone scan and CT was taken; every 3 months using blood tests the cases were followed-up. The samples were analyzed for estrogen, progesterone and HER2 Neu receptor and then survival analysis. Result:  All cases (n=160) were divided into two equal treatment groups (Mean age: 47.4 years). While no significant difference was  found between the two groups for age, estrogen, progesterone, HER2 Neu receptor, size/type of tumor, number of lymph node and metastasis rate, the mortality and exacerbation rates in Anthracycline group (12.5% and 7.5%, respectively) was significantly higher compared to Taxane group (3.8% and 0, respectively). In addition, the survival rate in Taxane group was higher. Conclusions:  Use of Taxane for BC reduces the mortality and exacerbation rates but increases its survival rate and life expectancy.}, Keywords = {Survival rate, Anthracycline, Taxane, Breast cancer}, volume = {20}, Number = {2}, pages = {180-187}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3031-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3031-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} } @article{ author = {Shiasi-Arani, Kobra and Moraveji, Seyyed Alireza and Zahavi, Zahr}, title = {Comparing the predicted final and target height in girls with pubertal onset between 7 and 9.5 years of age}, abstract ={Background: One of the main concerns about the early puberty is short final height. In cases comparing the predicted final and target height can help predicting this problem. In this study we compared the target and predicted adult height in girls with early puberty. Material and Methods: A cross-sectional study was carried out on girls )n=200( with early Puberty. The diagnosis of early puberty in girls was made based upon the onset of secondary sexual characteristics between 7-9.5 years of age. The weight, height, BMI and pubertal stage were determined. Marshall-Tanner method was used for sexual maturation staging. Greulich-Pyle and Bayley-Pinneau methods were also used for bone age determination and prediction of adult height, respectively. Target height was calculated using the Mean of their parental heights minus 6.5. Results: Among the qualified participants (n=200, mean age 8.44±0.72 years) 90 cases (45.5%) reffered with thelarche, 17 (8.5%) with pubarche and 92 (46%) with both thelarche and pubarche. The Mean predicted adult height and target height was156±6.69 and 156±4.3 cm, respectively. The 26 (13%) of cases had a predicted adult height lower than 150 cm and 15 (7.5%) that of lower than target zone. Conclusions: Although the majority of girls with early puberty have a predicted final adult height proportional to their target height, but some of these girls are at risk of low adult height.}, Keywords = {Bone Age Measurement, Precocious puberty, Height, Children, Growth}, volume = {20}, Number = {2}, pages = {188-195}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-3032-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-3032-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2016} }