@article{ author = {Esmaeili, Mohammad Hosein and Mafe-Esmaeili, Mahi}, title = {The effect of metformin on memory retention of inhibitory avoidance learning in streptozotocin-induced rat model of alzheimer’s disease}, abstract ={Background: Alzheimer’s disease (AD) is closely associated with impaired insulin signaling in brain, suggesting it to be a brain-specific form of diabetes and thus termed as the “type 3 diabetes”. Metformin is one of the most widely used agent against insulin resistance. The present study aimed to investigate the therapeutic effect of metformin on memory retention in streptozotocin (STZ)-induced model of AD in rats using an inhibitory avoidance learning. Materials and Methods: In this experimental study, 56 male Wistar rats were randomly allocated into 7 groups: control STZ-treated, STZ plus saline or metformin. For the induction of AD, STZ (3 mg/kg) was administered bilaterally into lateral ventricles. All rats were trained in shuttle box for inhibitory avoidance learning. Metformin (50, 100, 200 mg/kg, i.p) or saline were injected immediately after the training. The retention test was performed after two days. Results: The results showed that post-training injection of metformin dose-dependently increased the time spent in the light chamber before entering to the dark chamber in STZ-treated rats. Moreover, metformin dose-dependently improved the memory retention in the rats. Conclusion: Post-training injection of metformin dose-dependently enhanced the memory retention in STZ-induced model of AD.}, Keywords = {Alzheimer, Metformin, Inhibitory avoidance learning, Memory}, volume = {19}, Number = {1}, pages = {1-7}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2515-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2515-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Malek-Mohammadi, Razieh and Roghani, Mehrdad and Salami, Mahmou}, title = {The effect of aqueous extract of Melissa officinalis on the oxidative stress indices in the midbrain tissue}, abstract ={Background: Parkinson disease (PD) is a common neuropathologic disorder. It is resulted from degeneration of the dopaminergic neurons of the substantia nigra pars compacta. It is known that the increased level of oxidative stress leads to the degeneration of dopaminergic neurons. Due to the antioxidant activities of Melissa officinalis, this study aimed to assess the effect of aqueous extract of Melissa officinalis on the oxidative stress indices of the midbrain. Materials and Methods: Forty-eight male Wistar rats (weighing 250-300 g) were assigned to four groups including control (CON), control treated with the extract (CONE), leasioned (L) and leasioned treated with the extract (LE). The neurodegeneration was induced through the injection of 12.5 µg 6-hydroxydopamine (6-OHDA) in the left striatum. The CONE and LE groups were pretreated by 1 mg/kg/day of aqueous extract of Melissa officinalis. The extract treatment was repeated three times with a 24-hour interval and then the neurodegeneration was induced in the animals. Two weeks after the neurodegeneration, the plasma level of malondealdehyde (MDA), nitrite, and superoxide dismutase (SOD) were measured. Results: Results showed a significant increase in the level of MDA in the L group pretreatment with the extract considerably declined the plasma concentration of MDA. Moreover, the SOD activity was profoundly lower in the L compared to the CON group. The extract was ineffective on the SOD activity in the LE group. Conclusion: The oral administration of aqueous extract of Melissa officinalis (100 mg/kg) can diminish some stress oxidative inducers in the midbrain.}, Keywords = {Melissa officinalis, 6-OHDA, Malondealdehyde, Nitrite, Superoxide dismutase }, volume = {19}, Number = {1}, pages = {8-14}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2516-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2516-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Rahimi-Feyli, Peyman and Tajik, Parviz and Shafiei, Shiva and Dodel, Masoumeh and Arbabi, Fatemeh}, title = {The effect of poly L-lactic acid nanofiber on the induction of colony formation of frozen-thawed bovine spermatogonial stem cells in vitro}, abstract ={Background: Preparing a suitable condition for in vitro culture of spermatogonial stem cells can help discover all of the molecular mechanisms involving in spermatogenesis and pave the way for gene manipulation. This study aimed to evaluate the effect of a poly L-lactic acid (PLLA) nanofiber on the proliferation of frozen-thawed bovine spermatogonial stem cells in vitro. Materials and Methods: The isolated spermatogonial cells from the prepubertal bull were frozen-thawed and cultured in two groups: the control group (C) including the spermatogonial cells and the treatment group (T) including the spermatogonial cells seeded onto PLLA. The cells in both groups were cultured in DMEM supplemented with 5% fetal bovine serum and 40 ng/ml glial cell line-derived neurotropic factor (GDNF) for 2 weeks. Colony assay was conducted on days 4, 7, 10 and 13 after the beginning of the culture. Expression of spermatogonial genes (PLZF, BCL6, GFRα-1, VASA, Itgα6) in the culture was determined by reverse transcription polymerase chain reaction (RT-PCR) on the last day of the experiment. Results: The viability rates of fresh cells before and after the addition of cryoprotectant agent were 87.4±2.4 and 81.8±3.1, respectively which decreased to 65±1.7 after the thawing (P<0.01). Moreover, the results showed that the surface area of colonies derived from spermatogonia were significantly greater in the treatment group compared to the control group on day 13 (P<0.05). Finally, RT-PCR revealed the expression of all spermatogonial stem cell (SSC) markers in both groups. Conclusion: The PLLA nanofiber can provide a suitable microenvironment for frozen-thawed SSCs in an in vitro-culture system.}, Keywords = {Stem cell, Spermatogonia, Nanofiber, Freezing-thawing}, volume = {19}, Number = {1}, pages = {15-23}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2525-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2525-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Ahmadi, Rahim and Gohari, Azam and Hooshmand, Mohamm}, title = {The effect of noise stress on serum levels of LH, FSH and testosterone in male rats}, abstract ={Background: Recent studies have shown that stress can influence the reproductive function. The purpose of this study was to determine the effects of noise stress on serum levels of LH, FSH and testosterone in male rats. Materials and Methods: In this experimental study, 28 male Wistar rats were randomly divided into 4 groups (7 rats in each group) including control, and groups exposed to noise stress for 1, 3 and 6h/day. After 8 weeks, blood samples were obtained using the cardiac puncture method and serum levels of LH, FSH and testosterone were measured by the enzyme-linked fluorescent assay. Results: Serum levels of LH and FSH were significantly decreased in all groups exposed to noise stress compared to the control group (P<0.001). Serum level of testosterone was significantly decreased in groups exposed to noise stress for 1, 3 and 6h/day compared to the control group (P<0.05, P<0.01, P<0.001, respectively). Conclusion: Findings of this study show that noise stress can reduce the serum levels of LH, FSH and testosterone resulting in damage to reproductive system. Hence, preventing stress can play an important role in healthy reproductive function.}, Keywords = {Noise stress, LH, FSH, Testosterone, Male rat}, volume = {19}, Number = {1}, pages = {24-29}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2517-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2517-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Sepehri-Moghadam, Heshmat and Rahbarian, Raheleh and Sadoughi, Seyyed Damooghi}, title = {The effect of aqueous extract of Launaea acanthodes (Boiss.) O. Kuntze on the serum levels of insulin and blood glucose and histomorphological changes of pancreas in diabetic rats}, abstract ={Background: Diabetes is a metabolic disorder that appears by hyperglycemia resulting from defects in insulin secretion and pancreatic function. Considering the properties of the Launaea acanthodes, this study aimed to examine the effects of this plant on serum level of insulin and blood glucose and histomorphological changes of pancreas in diabetic rats.Materials and Methods: In this study, 27 male rats were allocated into the equal groups of control, diabetic control and experimental diabetic. The diabetes in diabetic control and experimental diabetic groups was induced using an intraperitoneal injection of alloxan. The experimental diabetic group received the aqueous extract of L. acanthodes (300 mg/kg, ip) in alternate days for one month. Sterile distilled water was injected to the animals of control and diabetic control groups. The serum levels of insulin and blood glucose were measured on days 1, 15 and 30. Histological studies were performed to determine the number and diameter of the pancreatic islets.Results: On days 15 and 30 of the experiment, injection of the L. acanthodes extract caused a significant decrease and increase in the serum level of glucose and insulin, respectively compared to the control group (P<0.05). Moreover, the mean number and diameter of pancreatic islets were significantly increased in the diabetic experimental compared to the diabetic control group (P<0.05). Conclusion: The extract of L. acanthodes can increase the serum insulin and the mean number and diameter of pancreatic islets and decrease the blood glucose in diabetic rats.}, Keywords = {Launaea acanthodes, Diabetes, Pancreas, Insulin, Glucose}, volume = {19}, Number = {1}, pages = {30-37}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2532-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2532-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Valipour-Chahardah-Charic, Saeed and Kesmati, Mahnaz and Vahdati, Akbar and Hoseiny, Seyyed Ebrahim}, title = {Oxidative stress indices in rat hippocampus using the memory deficit model induced by zinc oxide nanoparticles}, abstract ={Background: Some beneficial effects of zinc oxide nanoparticles (nano ZnO) in medicine have been widely considered in the food and pharmaceutical industries. Despite its beneficial effects and the contradictory oxidant and antioxidant properties of these nano ZnO on cognitive and memory processes, a challenge would be on the medicinal application of these nanoparticles. This study aimed to evaluate the parameters of hippocampal oxidative stress in memory deficits induced by nano ZnO. Materials and Methods: In this study, 28 adult male Wistar rats (200-250g) were allocated into 4 groups (n=7): control (Saline injection) and three experimental groups with different doses of ZnO (1.25, 2.5 and 5 mg/kg). Long-term memory in an inhibitory avoidance paradigm was examined at one day after the treatment and training. The malondialdehyde (MDA) level as an index of lipid peroxidation and the total thiols (- SH) were evaluated using the biochemical analysis of the hippocampus. Results: Nano ZnO at 1.25, 5 and also 2.5 mg/kg resulted in the impairment of memory (P<0.001 and P<0.01, respectively) with no change in the locomotor activity a decrease in thiols levels in groups received the higher doses of nano ZnO (2.5 and 5 mg/kg) and an increase in the thiols levels in groups that received the highest dose of nano ZnO wereseen (P<0.001). Conclusion: It can be concluded that memory impairment induced by nano ZnO in rats may not be attributed to the effects of oxidative stress in the hippocampus.}, Keywords = {Nano zinc oxide, Avoidance memory, Oxidative stress, Rat}, volume = {19}, Number = {1}, pages = {38-46}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2538-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2538-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Zamani, Batol and Moravveji, Sayyed Alireza and Ehteram, Hasan and Shokrani, Marjaneh}, title = {Correlation between the serum level of IL-17 and rheumatoid arthritis}, abstract ={Background: Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease. Interleukin-17 (IL-17) has an important role in the pathogenesis of RA. This study aimed to evaluate the correlation between the serum level of IL-17 and the disease activity. Materials and Methods: In this cross-sectional study, 60 patients fulfilled the American college of rheumatology criteria for RA were recruited. Demographic data of patients including age, gender, and disease duration were recorded and then the serum was taken and analyzed for IL-17 level. The disease activity score (DAS28-ESR) was calculated for all patients and the scores lower than 2.6 were considered as inactive 2.6 to 3.1 as mild, 3.2 to 5 as moderate and higher than 5 were considered as the severely active. Results: Of 60 patients studied, 23 cases had controlled RA with mean disease duration of 75.37 months and mean IL-17serum level of 144.81±47.83 ng/l and 37 cases had active RA with mean disease duration of 101.78 months and the mean serum level of 237±93.8 ng/l. There was a significant correlation between the serum IL-17 level and disease activity (P<0.001) as well as between the serum level of IL-17 and numbers of inflamed and tender joints, the ESR and visual analogue scale. In patients with active RA, the level of serum IL-17 was significantly higher in severe as compared to mild and moderate activity disease. Conclusion: There is a correlation between the serum level of IL-17 and disease activity score. Therefore, the IL-17 level is an indicator of the disease activity and severity of RA and can be used in follow-up of the disease.}, Keywords = {Rheumatoid arthritis, Serum IL-17, Disease activity score (DAS28-ESR)}, volume = {19}, Number = {1}, pages = {47-53}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2518-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2518-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Abbasi, Ali and Delavari, Mehdi and Arbabi, Mohsen and Arj, Abbas and Doroodgar, Masoud and Taherian, Ali Akbar and Doroodgar, Moein and Nikoueinejad, Hasan and Pourbabaee, Mohammad and Doroodgar, Abbas}, title = {The effect of tamoxifen on the growth of Leishmania major promastigotes and amastigotes in vitro}, abstract ={Background: Treatment of cutaneous leishmaniasis with pentavalent antimony compounds, as an established drug, may have limitations, side effects and recurrence risk. For this reason, finding new and effective drugs is of great importance. In the present study, the effect of tamoxifen on the growth of Leishmania major promastigotes and amastigotes was evaluated in vitro. Materials and Methods: In this experimental study, the effect of different concentrations (1, 5, 10, 20 and 50 μg/ml) of tamoxifen on Leishmania promastigotes and amastigotes were evaluated in three different times (24, 48 and 72h) and the inhibitory concentration 50 (IC50) was calculated by counting of the parasites. The MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 biphenyltetrazolium bromide) assay was used to determine the percentage of live promastigotes and amastigotes after adding tamoxifen. Results: The number of promastigotes and amastigotes were declined in the presence of various concentrations of tamoxifen after 24, 48 and 72 hours of culturing. Twenty-four hours after culturing, the number of parasites was 1.07×106 per ml in the control group and the parasite numbers in the concentrations of 1 and 50 μg/ml tamoxifen were 0.95×106 and 0.06×106, respectively. The IC50 value of tamoxifen was 2.64μg/ml. Conclusion: Tamoxifen has antileishmanial effects in vitro thus, more researches on the effect of tamoxifen in animal models are suggested.}, Keywords = {Leishmania major, Amastigote, Promastigote, Tamoxifen}, volume = {19}, Number = {1}, pages = {54-59}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2528-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2528-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Eivazi-Arvanagh, Neda and Chakerzehi, Arezo and Hemati, Mahdieh and Zarinnehad, Hananeh and Moradi, Ali}, title = {The effect of melittin on the inhibition of Rac1 expression in HeLa cervical cancer cell lines}, abstract ={Background: Cell migration plays a principal role in many biological and pathological processes, including embryonic and tumor metastasis. It is well-known that Rac1 from Rho GTPase family is the key regulator of the cell migration. Melittin is a natural peptide in bee venom with apoptotic and anti cancer effects. This study aimed to examine the effect of melittin on Rac1 expression. Materials and Methods: HeLa cells were cultured in Dulbecco's modified Eagle's medium until they reached 80% confluency, then treated with melittin (0.5 and 1 μg/ml) with the consideration of IC50=1 μg/ml for 6 hours. In the control group the same volume of distilled water, as the melittin solvent, was added. Thereafter, cells were lyzed with buffer and after centrifugation the protein concentration of supernatant was measured using the Bradford method. The amount of Rac1 expression was determined using the Western blotting technique and chemiluminescence. Outcomes were normalized with β-Actin as an internal control and the results were displayed as the percentage against the control. Results: The Rac1expression compared to the control (100%), in the presence of different concentrations of melittin (0.5 and 1 μg/ml) were 13.88%±1.15% and 1.7%±0.96%, respectively which showed a significant reduction in Rac1 melittin expression (P<0.05). Conclusion: It can be concluded that melittin can dose-dependently inhibit the Rac1 expression in HeLa cell line.}, Keywords = { Rac1, Cell migration, Melittin, HeLa cells}, volume = {19}, Number = {1}, pages = {60-66}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2520-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2520-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Sadat-Larijani, Mona and Nikbin, Mehri and Bakhshi-Khaniki, Gholam Reza and Talebi, Solmaz and Aghasadeghi, Mohammad Reza and Sadat, Sayyed Mehdi}, title = {Relationship between the IL28B gene-related single-nucleotide polymorphism of rs8099917 and susceptibility to hepatitis C infection in Iranian population}, abstract ={Background: The current medical treatment for hepatitis C is a combination of antiviral therapy along with pegylated interferon alpha and ribavirin. Recent studies have demonstrated that single nucleotide polymorphisms near the interleukin 28B gene coding for IFN-λ3 were associated with the antiviral response. Therefore, this study aimed to determine the frequency of G/T polymorphism of rs8099917 among the Iranian population. Materials and Methods: This cross-sectional study was performed on 93 blood samples (71 sensitive and 22 resistant to treatment) collected from individuals suffering from chronic HCV, and 57 healthy controls. DNA was extracted from the samples and the frequency of the polymorphism was analyzed using the PCR-RFLP method. Finally, the products were detected on 3.5% agarose gel electrophoresis.Results: The frequency of the G/T polymorphism between the healthy individuals and patients were TT: 75%, TG: 23%, GG: 2%, and TT: 57%, TG: 35%, GG: 8%, respectively. Moreover, the TT genotype was identified in 46 patients of whom 71 achieved SVR, while the GT heterozygous was found in 33 patients and SVR was achieved in 19. Finally, the GG was detected in 7 patients and only one patient was resistant to treatment. Conclusion: Results show a significant effect of G allele on susceptibility to HCV compared to the other allele T (P=0.013). Although no correlation was seen between the polymorphism and SVR among the patients, further studies with more samples are necessary.}, Keywords = {Hepatitis C, IL-28B gene, Polymorphism, rs8099917}, volume = {19}, Number = {1}, pages = {67-75}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2536-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2536-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Lotfi, Mohammad Hasan and Rahimi-Pordanjani, Saj}, title = {Comparison of the growth patterns among children with congenital hypothyroidism and healthy children in Yazd city in 2014: a historical cohort study}, abstract ={Background: The congenital hypothyroidism is one of the most common endocrine diseases, which can affect the growth of the children. This study aimed to compare the growth patterns among the children with congenital hypothyroidism (CH) and healthy children in Yazd, Iran. Materials and Methods: This retrospective cohort study was performed on all neonatal children with CH, who had been born since 2006 to 2008. The average height, weight and head circumference along with the ratio of variations of these variables were measured and compared in the children with CH and healthy children during the first 5 years of life after considering their age and gender. Results: Results showed no significant difference in the average height, weight and head circumference between the girls with CH and the healthy ones. Moreover, there was a significant difference in the average height of boys at the age of 3 to 24 months, the average weight at the age of 6 to 18 months and the average head circumference at the age of 12 to 18 months compared with the values of healthy children in this city. At the end of a 5-year follow-up, no significant difference was observed in the growth disorder ratio for weight, height and head circumference between both the healthy children and patients in both genders. Conclusion: After treatment and care for these patients, the growth patterns of these children would be within the normal and desirable range.}, Keywords = {Congenital hypothyroidism, Children, Height, Weight, Head circumference}, volume = {19}, Number = {1}, pages = {76-85}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2521-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2521-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} } @article{ author = {Ghasemi, Fatemeh Sadat and Rasti, Sima and Piroozmand, Ahmad and Fakhrie-Kashan, Zohreh and Mousavi, Sayyed Gholam Abbas}, title = {Comparison of the Frequency of anti- CMV, - Rubella and -HSV antibodies in women with spontaneous abortion and normal delivery}, abstract ={Background: This study aimed to determine the prevalence of serum IgG and IgM antibodies against cytomegalovirus (CMV), rubella and herpes simplex virus (HSV) in the women with spontaneous abortion compared to women with normal delivery. Materials and Methods: This case-control study was performed on 81 women with spontaneous abortion (case) and 98 women with normal delivery (control) referred to the obstetrics and gynecology clinics of Tehran hospitals in 2013. All the serum samples were tested for CMV, HSV and rubella IgM and IgG antibodies using ELISA kits. The data along with demographics and the gestational age of the mothers were recorded in the questionnaire and analyzed using SPSS. Results: The positive IgG antibodies against CMV were 87.7% (n=71) and 90.8% (n=89) in the case and control groups, respectively. Moreover, the positive IgM antibodies were 25.9% (n=21) and 12.2 %( n=12) in the case and control groups, respectively (P= 0.02). IgG antibodies against rubella in the case and control groups were 75.3% (n=61) and 86.7% (n=85), respectively. Also, IgM antibodies were 1.2% (n=1) and 2% (n=2), respectively (P=0.03). IgG antibody against herpes was 79% (n=64) and 85.7% (n=84) in the case and control groups, respectively IgM was positive (3.1%) in only 3 cases. Conclusion: Results of the present study reveal that the IgM antibody against CMV in case group was significantly higher than the control group, which shows the role of CMV in the occurrence of abortion.}, Keywords = {Cytomegalovirus, Rubella, Herpes simplex virus, Spontaneous abortion }, volume = {19}, Number = {1}, pages = {86-92}, publisher = {Kashan University of Medical Sciences}, url = {http://feyz.kaums.ac.ir/article-1-2539-en.html}, eprint = {http://feyz.kaums.ac.ir/article-1-2539-en.pdf}, journal = {Feyz Medical Sciences Journal}, issn = {1029-7855}, eissn = {2008-9821}, year = {2015} }