fa تاثیر یک دوره تمرین مقاومتی فزاینده بر برخی نشانگرهای آپوپتوزی در بافت قلب موش‌های صحرایی مبتلا به سندرم ترک مورفین medicine, paraclinic medicine, paraclinic پژوهشي Research <div style="text-align: justify;"><span style="font-size:12px;"><span style="line-height:2;"><span style="font-family:IRANyekan;"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span b="" lang="FA" mitra="">زمینه و هدف:</span></b><span b="" lang="FA" mitra=""> استفاده</span> <span b="" lang="FA" mitra="">از</span> <span b="" lang="FA" mitra="">داروهای مختلف</span> <span b="" lang="FA" mitra="">در</span> <span b="" lang="FA" mitra="">سندرم ترک</span> <span b="" lang="FA" mitra="">معمولاً</span> <span b="" lang="FA" mitra="">اثربخشی</span> <span b="" lang="FA" mitra="">کمی</span> <span b="" lang="FA" mitra="">دارند</span> <span b="" lang="FA" mitra="">و</span> <span b="" lang="FA" mitra="">سبب وابستگی</span> <span b="" lang="FA" mitra="">فرد به</span> <span b="" lang="FA" mitra="">مواد</span> <span b="" lang="FA" mitra="">جدید</span> <span b="" lang="FA" mitra="">می&shy; گردد.</span> <span b="" lang="FA" mitra="">هدف پژوهش حاضر تعیین اثر ۸ هفته تمرین مقاومتی بر سطح پروتئین‌های </span><span dir="LTR">Bax</span> <span b="" lang="FA" mitra="">و </span><span dir="LTR">Bcl-2</span><span b="" mitra=""> در بافت قلب موش‌های صحرایی مبتلا به سندرم ترک مورفین بود.</span> <b><span lang="FA" style="font-family:"B Mitra""></span></b></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span b="" lang="FA" mitra="">روش‌ها:</span></b><span b="" lang="FA" mitra=""> در</span> <span b="" lang="FA" mitra="">این</span> <span b="" lang="FA" mitra="">مطالعه</span> <span b="" lang="FA" mitra="">تجربی،</span> <span b="" lang="FA" mitra="">32</span> <span b="" lang="FA" mitra="">سر</span> <span b="" lang="FA" mitra="">موش صحرایی</span> <span b="" lang="FA" mitra="">نر</span> <span b="" lang="FA" mitra="">نژاد</span> <span b="" lang="FA" mitra="">ویستار</span> <span b="" lang="FA" mitra="">به طور</span> <span b="" lang="FA" mitra="">تصادفی</span> <span b="" lang="FA" mitra="">به</span> <span b="" lang="FA" mitra="">4</span> <span b="" lang="FA" mitra="">گروه</span> <span b="" lang="FA" mitra="">شامل</span> <span b="" lang="FA" mitra="">کنترل،</span> <span b="" lang="FA" mitra="">سندرم ترک،</span> <span b="" lang="FA" mitra="">و</span> <span b="" lang="FA" mitra="">تمرین مقاومتی فزاینده</span> <span b="" lang="FA" mitra="">و سندرم ترک</span> <span b="" lang="FA" mitra="">همراه</span> <span b="" lang="FA" mitra="">با</span> <span b="" lang="FA" mitra="">تمرین مقاومتی فزاینده تقسیم</span> <span b="" lang="FA" mitra="">شدند. اعتیاد به مورفین از طریق تجویز خوراکی</span> <span b="" lang="FA" mitra="">مورفین</span> <span b="" lang="FA" mitra="">سولفات 0/4 میلی‌گرم/دسی‌لیتر به</span> <span b="" lang="FA" mitra="">مدت 21 روز القا شد. برای</span> <span b="" lang="FA" mitra="">اطمینان</span> <span b="" lang="FA" mitra="">از</span> <span b="" lang="FA" mitra="">وابستگی</span> <span b="" lang="FA" mitra="">به</span> <span b="" lang="FA" mitra="">مورفین،</span> <span b="" lang="FA" mitra="">به تعدادی</span> <span b="" lang="FA" mitra="">از</span> <span b="" lang="FA" mitra="">حیوانات</span> <span b="" lang="FA" mitra="">نالوکسان </span><span lang="AR-SA"><span b="" mitra="">(</span></span><span dir="LTR">2mg/kg, ip</span><span lang="AR-SA"><span b="" mitra="">)</span></span><span b="" lang="FA" mitra=""> تزریق گردید. سپس گروه‌های تمرین به مدت 8 هفته در پروتکل تمرین مقاومتی شرکت کردند. بعد همه موش‌ها کشته و بافت قلب آنها خارج گردید. برای ارزیابی فاکتورهای آپوپتوزی از کیت‌های الایزا استفاده شد.</span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span b="" lang="FA" mitra="">یافته‌ها:</span></b> <span b="" lang="AR-SA" mitra="">افزایش معنی‌دار سطح پروتئین </span><span dir="LTR">Bax</span><span b="" lang="AR-SA" mitra=""> (پروآپپتوتیک) (0/03</span><span lang="AR-SA"><span b="" mitra="">=</span></span><span dir="LTR">P</span><span b="" lang="AR-SA" mitra="">) و کاهش معنی‌دار سطح پروتئین </span><span dir="LTR">Bcl-2</span><span b="" lang="AR-SA" mitra=""> (آنتی‌آپوپتوزی) (0/006</span><span lang="AR-SA"><span b="" mitra="">=</span></span><span dir="LTR">P</span><span b="" lang="AR-SA" mitra="">) در گروه مورفین در مقایسه با گروه کنترل سالم </span><span b="" lang="FA" mitra="">گزارش شد</span><span b="" lang="AR-SA" mitra="">. همچنین افزایش سطوح </span><span dir="LTR">Bcl-2</span><span b="" mitra=""> (0/043</span><span lang="AR-SA"><span b="" mitra="">=</span></span><span dir="LTR">P</span><span lang="AR-SA"><span b="" mitra="">)</span></span><span b="" lang="AR-SA" mitra=""> و کاهش پروتئین‌های </span><span dir="LTR">Bax</span><span b="" mitra=""> (0/01</span><span lang="AR-SA"><span b="" mitra="">=</span></span><span dir="LTR">P</span><span b="" lang="AR-SA" mitra="">)</span> <span b="" lang="AR-SA" mitra="">در گروه </span><span b="" lang="FA" mitra="">سندرم ترک</span> <span b="" lang="FA" mitra="">همراه</span> <span b="" lang="FA" mitra="">با</span> <span b="" lang="FA" mitra="">تمرین مقاومتی فزاینده </span><span b="" lang="AR-SA" mitra="">در مقایسه با گروه </span><span b="" lang="FA" mitra="">سندرم ترک</span> <span b="" lang="AR-SA" mitra="">تفاوت معنی‌دار را نشان داد.</span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span b="" lang="FA" mitra="">نتیجه‌گیری:</span></b> <span b="" lang="AR-SA" mitra="">تمرین</span><b> </b><span b="" lang="AR-SA" mitra="">مقاومتی </span><span b="" lang="FA" mitra="">فزاینده </span><span b="" lang="AR-SA" mitra="">احتمالاً می‌تواند بر میزان کاهش فاکتورهای آپوپتوتیک بافت قلب در موش‌های با سندرم ترک مورفین اثر مثبت و محافظتی داشته باشد.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="color:#000000;"><span style="line-height:115%"><span style="unicode-bidi:embed"><b><span style="line-height:115%">Background and Aim: </span></b><span lang="EN"><span style="line-height:115%">The use of different drugs in withdrawal syndrome usually has little effectiveness and causes the person to become dependent on new substances.</span></span><span style="line-height:115%"> The aim of the present study was to determine the effect of eight weeks of resistance training on the level of Bax and Bcl-2 proteins in the heart tissue of rats with morphine withdrawal syndrome.</span></span></span><br> <span style="line-height:115%"><span style="unicode-bidi:embed"><b><span style="line-height:115%">Methods:</span></b><span style="line-height:115%"> In this experimental study, 32 male Wistar rats were randomly divided into four groups including control, withdrawal syndrome, and incremental resistance training and incremental resistance training with withdrawal syndrome. Addiction to morphine was induced by oral administration of 0.4 mg/dL morphine sulfate for 21 days. To ensure morphine dependence, a number of rats were injected with naloxone (2mg/kg, ip). Then the training groups participated in the incremental resistance training protocol for eight weeks. Afterwards, rats were killed and their heart tissue was removed. ELISA kits were used to evaluate apoptotic factors.</span> <span style="line-height:115%"></span></span></span><br> <span style="line-height:115%"><span style="unicode-bidi:embed"><b><span style="line-height:115%">Results: </span></b><span style="line-height:115%">A significant increase in the level of Bax protein (proapoptotic) (P=0.03) and a significant decrease in the level of Bcl-2 protein (antiapoptotic) (P=0.006) were reported in the morphine group compared to the healthy control group. In addition, the increase in Bcl-2 level (P=0.043) and the decrease in Bax protein (P=0.01) in the incremental resistance training with the withdrawal syndrome group showed a significant difference compared to the withdrawal syndrome group. </span></span></span><br> <span style="line-height:115%"><span style="unicode-bidi:embed"><b><span style="line-height:115%">Conclusion:</span></b><span style="line-height:115%"> The incremental resistance training can probably have a positive and protective effect on the reduction of apoptotic factors in heart tissue in rats with morphine withdrawal syndrome.</span></span></span></span></span></span></div> آپوپتوز, بافت قلب, تمرین مقاومتی فزاینده, سندرم ترک مورفین Apoptosis, Heart tissue, Incremental resistance exercise, Morphine withdrawal syndrome 388 397 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4503-1&slc_lang=fa&sid=1 Maryam Sadegh joola مریم صادق ژولا sadeghimaryam911@gmail.com 2900319475328460042911 0009-0007-5988-5383 No Department of Physical Education, Borujard Branch, Islamic Azad University, Borujard, Iran گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران Abas Saremi عباس صارمی a-saremi@araku.ac.ir 2900319475328460042912 2900319475328460042912 Yes Sports Physiology Department, Faculty of Sports Sciences, Arak University, Arak, Iran گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه اراک، اراک، ایران Mojtaba Khansooz مجتبی خان سوز Mojtaba.khansooz@gmail.com 2900319475328460042913 2900319475328460042913 No Department of Physical Education, Arak Branch, Islamic Azad University, Arak, Iran گروه تربیت بدنی، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران