fa تاثیر تمرین تناوبی شدید همراه با مکمل خرفه بر آیریزین و TLR4 بافت کبد رت‌های مبتلا به کبد چرب غیر الکلی medicine, paraclinic medicine, paraclinic پژوهشي Research <div style="text-align: justify;"><span style="line-height:2;"><span style="font-family:IRANyekan;"><span style="color:#000000;"><span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><strong><span lang="AR-SA" style="font-size:9.0pt"><span b="" zar="">سابقه و هدف:</span></span> </strong><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">اصلاح سبک زندگی از طریق مداخلات رژیم غذایی </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">و</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">تمرین ‌ورزشی همچنان یکی از مؤثرترین راه‌های درمان کبد چرب ‌غیرالکلی است. هدف از تحقیق حاضر </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">تعیین</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> تاثیر تمرین تناوبی شدید همراه با مکمل خرفه بر آیریزین و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">TLR4</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> بافت کبد رت</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">‌</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">های مبتلا به کبد چرب غیر الکلی بود.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><span lang="AR-SA" style="font-size:9.0pt"><strong><span b="" zar="">مواد و روش &shy;ها:</span></strong><span b="" lotus=""> در این مطالعه تجربی، تعداد 30 موش‌صحرایی نژاد</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">ویستار نر با دامنه وزنی 185-160 گرم به دو گروه اصلی کبدچرب غیرالکلی (</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">2</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">5</span><span new="" roman="" times="">=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) و کنترل سالم (5</span><span new="" roman="" times="">=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) تقسیم شدند. کبد چرب‌ غیر الکلی با 12 هفته رژیم غذایی پرچرب القاء شد. پس از تایید ابتلا به کبد چرب ‌غیر الکلی (5</span><span new="" roman="" times="">=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) رت</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">‌های مبتلا</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> (</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">20</span></span><span lang="AR-SA" style="font-size:9.0pt"><span new="" roman="" times="">=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) به چهار زیرگروه کنترل کبد چرب (5=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">)، گروه مکمل</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""> خرفه </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">(5=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">)، گروه تمرین (5</span><span new="" roman="" times="">=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) و گروه تمرین+مکمل خرفه (5</span><span new="" roman="" times="">=</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">n</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) تقسیم شدند. مکمل خرفه به &shy;صورت روزانه و تمرین تناوبی شدید به مدت 8 هفته، 5 جلسه در هفته در گروه‌های مربوطه اعمال شد. مقادیر </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">آیریزین </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">TLR4</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">بافت کبد به روش الایزای ساندویچی اندازه‌گیری شد.</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><span lang="AR-SA" style="font-size:9.0pt"><strong><span b="" zar="">نتایج:</span></strong><span b="" lotus=""><strong> </strong>مقادیر آیریزین در گروه تمرین + مکمل خرفه </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">در مقایسه با</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> گروه کنترل کبد چرب (0/001</span><span new="" roman="" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">P</span></span></i><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">)، گروه تمرین تناوبی (0/018</span><span new="" roman="" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">P</span></span></i><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) و گروه مکمل خرفه (0/033</span><span new="" roman="" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">P</span></span></i><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) به&shy;طور معناداری بالاتر بود.</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">مقادیر </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">TLR4</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> در گروه تمرین + مکمل خرفه (0/007</span><span new="" roman="" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">P</span></span></i><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) و گروه تمرین تناوبی (0/045</span><span new="" roman="" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">P</span></span></i><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">) نسبت به گروه کنترل کبد چرب به&shy;طور معناداری پایین‌تر بود.</span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><strong><span lang="AR-SA" style="font-size:9.0pt"><span b="" zar="">نتیجه&shy; گیری:</span></span></strong> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">تمرین تناوبی شدید به‌همراه مصرف مکمل خرفه منجر به </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">افزایش</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="">آیریزین و کاهش </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">TLR4</span></span><span style="font-size:9.0pt"><span b="" lotus=""> بافت کبد رت‌های صحرایی مبتلا به کبدچرب غیرالکلی می‌گردد که ممکن است در بهبود کبدچرب غیرالکلی موثر باشد. </span></span><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="line-height:2;"><span style="font-family:Times New Roman;"><span style="color:#000000;"><span style="font-size:14pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="X-NONE" style="font-size:12.0pt">Background</span></b><span lang="X-NONE" style="font-size:12.0pt">: </span><span style="font-size:12.0pt">L</span><span lang="X-NONE" style="font-size:12.0pt">ifestyle modification through dietary interventions and exercise training is still </span><span style="font-size:12.0pt">one of</span> <span style="font-size:12.0pt">the</span><span lang="X-NONE" style="font-size:12.0pt"> most effective way</span><span style="font-size:12.0pt">s</span><span style="font-size:12.0pt"> to treat non-alcoholic fatty liver disease. The aim of this </span><span style="font-size:12.0pt">study</span><span lang="X-NONE" style="font-size:12.0pt"> was to investigate </span><span style="font-size:12.0pt">t</span><span lang="X-NONE" style="font-size:12.0pt">he effect of </span><span style="font-size:12.0pt">a </span><span lang="X-NONE" style="font-size:12.0pt">high</span><span style="font-size:12.0pt">-</span><span lang="X-NONE" style="font-size:12.0pt">intensity interval training </span><span style="font-size:12.0pt">along </span><span lang="X-NONE" style="font-size:12.0pt">with <i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplementation</span><span style="font-size:12.0pt"> on</span><span lang="X-NONE" style="font-size:12.0pt"> Irisin and TLR4 in the liver tissue</span><span style="font-size:12.0pt">s</span><span lang="X-NONE" style="font-size:12.0pt"> of rats with non-alcoholic fatty liver disease</span><span style="font-size:12.0pt">.</span><span style="font-size:12.0pt" lang="X-NONE"></span></span></span></span><br> <span style="font-size:14pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="X-NONE" style="font-size:12.0pt">Materials and Methods</span></b><span lang="X-NONE" style="font-size:12.0pt">: In this experimental study, 30 male Wistar rats were divided into two groups: non-alcoholic fatty liver </span><span style="font-size:12.0pt">disease </span><span lang="X-NONE" style="font-size:12.0pt">(n=25) and healthy control (n=5).</span> <span lang="X-NONE" style="font-size:12.0pt">Non-alcoholic fatty liver was induced by 12 weeks of high-fat diet. After confirming non-alcoholic fatty liver disease, rats (n=20) were </span><span style="font-size:12.0pt">assigned</span><span lang="X-NONE" style="font-size:12.0pt"> into four subgroups</span><span style="font-size:12.0pt">;</span> <span style="font-size:12.0pt">non-alcoholic </span><span lang="X-NONE" style="font-size:12.0pt">fatty liver control group (n=5), <i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplement group (n=5), </span><span style="font-size:12.0pt">training</span><span lang="X-NONE" style="font-size:12.0pt"> group (n=5) and </span><span style="font-size:12.0pt">training</span><span style="font-size:12.0pt"> group + <i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplement (n=5). <i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplement was </span><span style="font-size:12.0pt">given</span><span lang="X-NONE" style="font-size:12.0pt"> daily and </span><span style="font-size:12.0pt">a </span><span lang="X-NONE" style="font-size:12.0pt">high</span><span style="font-size:12.0pt">-</span><span lang="X-NONE" style="font-size:12.0pt">intensity interval training </span><span style="font-size:12.0pt">was presented </span><span lang="X-NONE" style="font-size:12.0pt">for 8 weeks, 5 sessions per week. The amounts of Irisin and TLR4 in liver tissue were measured</span> <span style="font-size:12.0pt">using the</span><span style="font-size:12.0pt"> sandwich ELISA method.</span> <span dir="RTL" style="font-size:12.0pt" lang="FA"></span></span></span></span></span><br> <span style="font-size:14pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><span style="color:#000000;"><b><span lang="X-NONE" style="font-size:12.0pt">Results</span></b><span lang="X-NONE" style="font-size:12.0pt">: Irisin levels were significantly higher in the </span></span><a name="_Hlk140917966"><span style="color:#000000;"><span style="font-size:12.0pt">training</span></span></a><span style="color:#000000;"><span lang="X-NONE" style="font-size:12.0pt">+<i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplement </span><span lang="X-NONE" style="font-size:12.0pt">group compared to the fatty liver control group (<i>P</i>=0.001)</span><span style="font-size:12.0pt">, </span><span lang="X-NONE" style="font-size:12.0pt">the interval training group (<i>P</i>=0.018) and <i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplement group (<i>P</i>=0.033). </span><span style="font-size:12.0pt">The </span><span lang="X-NONE" style="font-size:12.0pt">TLR4</span><span style="font-size:12.0pt"> levels</span><span lang="X-NONE" style="font-size:12.0pt"> in the </span><span style="font-size:12.0pt">training</span><span lang="X-NONE" style="font-size:12.0pt">+<i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplement group (<i>P</i>=0.007) and the high intensity interval training group (<i>P</i>=0.045) were significantly lower than the fatty liver control group.</span></span></span></span></span><br> <span style="color:#000000;"><span style="font-size:14pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b><span lang="X-NONE" style="font-size:12.0pt">Conclusion</span></b><span lang="X-NONE" style="font-size:12.0pt">: </span><span style="font-size:12.0pt">H</span><span lang="X-NONE" style="font-size:12.0pt">igh</span><span style="font-size:12.0pt">-</span><span lang="X-NONE" style="font-size:12.0pt">intensity interval training along with <i>Portulaca </i></span><i><span style="font-size:12.0pt">o</span></i><i><span lang="X-NONE" style="font-size:12.0pt">leracea</span></i><span lang="X-NONE" style="font-size:12.0pt"> supplementation leads to increase</span><span style="font-size:12.0pt">s</span><span lang="X-NONE" style="font-size:12.0pt"> in Irisin</span><span style="font-size:12.0pt"> release</span><span lang="X-NONE" style="font-size:12.0pt"> and decrease</span><span style="font-size:12.0pt">s</span><span lang="X-NONE" style="font-size:12.0pt"> in </span><span style="font-size:12.0pt">the </span><span lang="X-NONE" style="font-size:12.0pt">TLR4 </span><span style="font-size:12.0pt">levels</span><span lang="X-NONE" style="font-size:12.0pt"> in the liver tissue of rats with non-alcoholic fatty liver disease, which may be effective in improving non-alcoholic fatty liver disease</span><span style="font-size:12.0pt">.</span></span></span></span></span></span></span></div> تمرین تناوبی با شدت بالا, خرفه, بیماری کبد چرب غیر الکلی, آیریزین, TLR4 High-intensity interval training, Portulaca oleracea, Non-alcoholic fatty liver disease, Irisin, TLR4 244 253 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4464-1&slc_lang=fa&sid=1 Majid Farah Nia مجید فرح نیا M.farahnia@yahoo.com 2900319475328460042986 2900319475328460042986 No Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, I.R. Iran. گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران Mohammad Raza Hosseinabadi محمدرضا حسین آبادی mrhoseinabadi@gmail.com 2900319475328460042987 2900319475328460042987 Yes Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, I.R. Iran. گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران Mehdi Zarei مهدی زارعی Mehdizarei@neyshabur.ac.ir 2900319475328460042988 2900319475328460042988 No Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, University of Neyshabur, Neyshabur, I.R. Iran. گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه نیشابور، نیشابور، ایران Mahmood Soltani محمود سلطانی Soltani.mahmood@gmail.com 2900319475328460042989 2900319475328460042989 No Department of Physical Education, Mashhad Branch, Islamic Azad University, Mashhad, I.R. Iran. گروه تربیت بدنی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران