fa ارتباط بین پلی مورفیسم rs896854ژنtp53inp1 و دیابت نوع 2 در شهر دلیجان medicine, paraclinic medicine, paraclinic پژوهشي Research <div style="text-align: justify;"><span style="color:#000000;"><span style="font-family:IRANyekan;"><span style="line-height:2;"><span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><span lang="AR-SA" style="font-size:9.0pt"><strong><span b="" zar="">سابقه و هدف:</span></strong><span b="" lotus=""><strong> </strong>مطالعات مهندسی ژنتیک بر <span style="letter-spacing:-.1pt">روی ژن </span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">tp53inp1</span></span></span></i><span style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> مشخص کرده که اختلال میتوفاژی میتوکندری&shy;های ناکارآمد، منبع اصلی استرس اکسیداتیو مزمن بوده و منجر به افزایش استعداد ابتلا به </span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt">دیابت</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt">می‌شود. هدف این مطالعه ارزیابی ارتباط پلی&shy;مورفیسم</span></span></span> <span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">rs896854</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> در ژن</span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">tp53inp1</span></span></span></i><span style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> و دیابت نوع 2 بود.</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><strong><span lang="AR-SA" style="font-size:9.0pt"><span b="" zar=""><span style="letter-spacing:-.1pt">مواد و روش &shy;ها:</span></span></span> </strong><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt">در این مطالعه موردی-شاهدی، نمونه&shy; های خون از 124 فرد بیمار مبتلا به دیابت نوع 2 و 100 فرد سالم از ساکنان شهر دلیجان، استان مرکزی تهیه شد</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt">. پس از استخراج </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">DNA</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> ژنومی با روش </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">Salting out</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt">،</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> جایگاه </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">rs896854</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> در ژن </span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">tp53inp1</span></span></span></i><span style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"> با استفاده از روش </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times=""><span style="letter-spacing:-.1pt">&nbsp;Tetra-Primer Arms-PCR</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt">ارزیابی گردید. </span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><span lang="AR-SA" style="font-size:9.0pt"><strong><span b="" zar="">نتایج:</span></strong><span b="" lotus=""><strong> </strong>فراوانی ژنوتیپ&shy; های </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">TT</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">CC</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">، </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">TC</span></span><span style="font-size:9.0pt"><span b="" lotus=""> به &shy;ترتیب در گروه بیمار 13، 29 و 58 درصد و در گروه کنترل 12، 17 و 71 درصد بودند. به علاوه فراوانی آلل &shy;های </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">T</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">C</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus=""> به &shy;ترتیب در گروه بیمار 42 درصد و 58 درصد و در گروه کنترل 5/47 درصد و 5/52 درصد بود.</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""> آنالیز آماری با آزمون مربع کای مشخص نمود که پلی&shy;مورفیسم </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">rs896854</span></span></i><span style="font-size:9.0pt"><span b="" lotus=""> در ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">tp53inp1</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">، ارتباط معناداری با بروز دیابت در جمعیت مورد مطالعه نداشت (0/05<</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">P</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">)</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri=""><strong><span lang="AR-SA" style="font-size:9.0pt"><span b="" zar="">نتیجه &shy;گیری:</span></span> </strong><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">طبق یافته‌های مطالعه حاضر، </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">وجود آلل </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">T</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus=""> یا </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">C</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">در جایگاه </span></span>&nbsp;<span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">rs896854</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">در ژن </span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" times="">tp53inp1</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="">، با احتمال ابتلا به دیابت نوع 2 ارتباط معنی‌داری نداشت</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="">. برای تائید این یافته‌ها، نیاز به بررسی بیشتر در جمعیت&shy; های مختلف با حجم نمونه بالاتر، ضروری است.</span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="line-height:2;"><span style="font-family:Times New Roman;"><span style="color:#000000;"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Background:</b> Genetic engineering studies on the <i>TP53INP1</i> gene have determined that a dysfunctional mitochondrial mitophagy disorder is the main source of chronic oxidative stress and leads to an increased susceptibility to diabetes. The aim of this study was to evaluate the relationship between rs896854 polymorphism of TP53INP1 gene and type 2 diabetes.</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Materials and Methods:</b> In this case-control study, blood samples were collected from 124 patients with type 2 diabetes and 100 healthy individuals from the residents of Delijan City, Markazi Province, Iran, in 2022. After extracting genomic DNA by the salting out method, the rs896854 location in <i>the TP53INP1</i> gene was evaluated using the Tetra-Primer Arms-PCR method.</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Results:</b> The frequency rates of TT, CC, and TC genotypes were 13%, 29%, and 58% in the patient group and 12%, 17%, and 71% in the control group, respectively. In addition, the frequency rates of T and C alleles were 42% and 58% in the patient group and 47.5% and 52.5% in the control group, respectively. Statistical analysis using the Chi-square test revealed that the rs896854 polymorphism of <i>TP53INP1</i> gene had no significant relationship with the occurrence of diabetes in the studied population (<i>P<0.05</i>).</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Conclusion:</b> According to the current findings, the presence of T or C allele at rs896854 location in TP53INP1 gene had no significant relationship with the probability of the occurrence of type 2 diabetes. To confirm these findings, it is necessary to carry out further investigations in different populations with a larger sample size.</span></span></span></span><br> &nbsp;</span></span></div> دیابت نوع 2, پلی­مورفیسم rs896854, ژن tp53inp1 Type 2 diabetes, Rs896854 polymorphism, TP53INP1 gene 306 314 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4394-1&slc_lang=fa&sid=1 Niloofar Zahedian نیلوفر زاهدیان niloofarzahedian20@gmail.com 2900319475328460043014 2900319475328460043014 No Department of Biology, Faculty of Basic Sciences, East Tehran Branch (Ghiamdasht), Islamic Azad University, Tehran, I.R. Iran. گروه ژنتیک، دانشکده علوم پایه، واحد تهران شرق، دانشگاه آزاد اسلامی تهران، ایران Maryam Salavatifar مریم صلواتی فر salavati@ari.ac.ir 2900319475328460043015 2900319475328460043015 Yes Department of Aerospace Physiology, Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, I.R. Iran. پژوهشگاه هوافضا، وزارت علوم، تحقیقات و فناوری، تهران، ایران Faranak Jamshidian فرانک جمشیدیان fjamshidian@gmail.com 2900319475328460043016 2900319475328460043016 No Department of Biology, Faculty of Basic Sciences, East Tehran Branch (Ghiamdasht), Islamic Azad University, Tehran, I.R. Iran. گروه ژنتیک، دانشکده علوم پایه، واحد تهران شرق، دانشگاه آزاد اسلامی، تهران، ایران