fa بررسی اثر هشت هفته مصرف تستوسترون انانتات بر فعالیت آنتی‌اکسیدانی و بیان ژن‌های NF-KB و سیکلواکسیژناز-2 بافت کلیه موش‌های صحرایی نر تمرین‌کرده مقاومتی عمومى General پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><strong><span b="" style="font-family:" zar="">سابقه و هدف:</span></strong><span b="" lotus="" style="font-family:"><strong> </strong>استروئیدهای آنابولیک - آندروژنیک می‌توانند به اختلال در سیستم آنتی‌اکسیدانی و برخی از نشانگرهای التهابی، <span style="letter-spacing:-.1pt">مانند فاکتور هسته‌ای کاپا </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">B</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">NF-KB</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) و سیکلواکسیژناز -2 </span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">(</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">COX- 2</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">) در</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> کلیه ورزشکاران </span>منجر<span style="letter-spacing:-.1pt"> شوند. هدف</span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">مطالعه حاضر،</span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">بررسی اثر هشت هفته مصرف تستوسترون انانتات بر فعالیت آنتی‌اکسیدانی بیان ژن‌های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">NF-KB</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">COX- 2</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> در </span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">بافت کلیه موش‌های صحرایی نر تمرین‌کرده مقاومتی بود.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><strong><span b="" style="font-family:" zar="">مواد و روش‌ها:</span></strong><span b="" lotus="" style="font-family:"><strong> </strong>در این پژوهش، تعداد</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">24</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">سر</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">موش</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">صحرایی</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">نر، پس</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">از</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">یک</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">هفته</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">آشناسازی</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">به</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">سه</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">گروه</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کنترل، تمرین و تمرین+ تستوسترون تقسیم شدند. موش‌های صحرایی گروه‌های تمرین و تمرین + تستوسترون به‌مدت هشت هفته و پنج جلسه در هفته تمرینات مقاومتی را انجام دادند، همچنین موش‌های صحرایی گروه تمرین + تستوسترون، سه روز در هفته دوز تجمعی هفتگی (</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">mg/kg</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> 20) تستوسترون انانتات را به‌صورت تزریق عضلانی دریافت نمودند. </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">جهت تجزیه‌وتحلیل یافته‌های پژوهش از آزمون آنالیز واریانس یک‌طرفه استفاده شد (05/0</span><span new="" roman="" style="font-family:" times="">&ge;</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">).</span></span><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><strong><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نتایج:</span></span> </strong><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">نتایج</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تحلیل</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">واریانس</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">یک‌طرفه</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">نشان</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">داد</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">که</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">بیان ژن </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">COX- 2</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> در گروه‌های تمرین (0/003</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) و تمرین+تستوسترون (0/001</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span></i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">). نسبت به گروه کنترل افزایش معنی‌دار داشت. همچنین افزایش معنی‌داری در گروه تمرین+تستوسترون نسبت به گروه تمرین مشاهده شد (0/001</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">). بیان ژن </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NF-kB</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> در گروه‌های تمرین (001/0</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">=</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) و تمرین+تستوسترون (0/001</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span></i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) در مقایسه با گروه کنترل به‌طور معنی‌داری بالاتر بود. همچنین افزایش معنی‌داری در گروه تمرین+تستوسترون نسبت به گروه تمرین مشاهده شد (0/001</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span></i><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="AR-SA" style="font-size:9.0pt"><strong><span b="" style="font-family:" zar="">نتیجه‌گیری:</span></strong><span b="" lotus="" style="font-family:"><strong> </strong>به‌نظر می‌رسد </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تمرینات مقاومتی شدید و </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">مصرف تستوسترون می‌تواند به افزایش عوامل التهابی در بافت کلیه موش‌های صحرایی نر منجر شود.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="color:#000000;"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><strong>Background: </strong>Anabolic-androgenic steroids can cause disturbances in the antioxidant system and some inflammatory markers such as nuclear factor kappa B (NF-KB) and cyclooxygenase-2 (COX-2) in kidney of athletes. So, this study aimed to investigate the effect of eight weeks of testosterone enanthate consumption on antioxidant activity, NF-KB and COX-2 genes expression in kidney tissue of resistance-trained male rats.</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><strong>Materials and Methods:</strong> 24 male rats were divided into three groups: control, training, training + testosterone after a week of familiarization. Rats in the training and training + testosterone groups performed resistance training for eight weeks and five sessions per week. Also, rats in the training + testosterone group received 20 mg/kg/week of testosterone enanthate by intramuscular injection three days a week. One-way analysis of variance was used to analyze the research findings (<i>P</i>&le;0.05).<span dir="RTL" style="font-family:"B Lotus"" lang="AR-SA"></span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><strong>Results: </strong>The results of one-way analysis of variance showed that COX-2 gene expression in the training group (<i>P</i>=0.001) and the training + testosterone group (<i>P</i>=0.001) had a significant increase compared to the control group. Also, a significant increase was observed in the Training + testosterone group compared to the training group (<i>P</i>=0.001). NF-kB gene expression in the training group (<i>P</i>=0.003) and the training + testosterone group (<i>P</i>=0.001) compared to the control group significantly was higher. Also, a significant increase was observed in the training + testosterone group compared to the training group (<i>P</i>=0.002).</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><strong>Conclusion:</strong> It seems that intense resistance training and testosterone consumption can lead to an increase in inflammatory factors in the kidney tissue of resistance trained rats.</span></span></span></span></span></div> استرس اکسیداتیو, NF-κB, COX-2, تستوسترون انانتات, تمرین مقاومتی Oxidative stress, NF-kB, COX-2, Testosterone enanthate, Resistance training 666 675 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4273-1&slc_lang=fa&sid=1 Masoumeh Mehrabi معصومه مهرابی masoumeh.mehrabi2012@yahoo.com 2900319475328460042244 2900319475328460042244 No Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Islamshahr, I.R. Iran. گروه فیزیولوژی ورزشی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران Yaser Kazemzadeh یاسر کاظم زاده yaser.kazemzadeh@yahoo.com 2900319475328460042245 2900319475328460042245 Yes Graduated from Kharazmi University, Tehran, I.R. Iran. دانش‌آموخته دانشگاه خوارزمی، تهران، ایران Ali Gorzi علی گرزی aligorzi1982@gmail.com 2900319475328460042246 2900319475328460042246 No Department of Sport Sciences, University of Zanjan, Zanjan, I.R. Iran. گروه فیزیولوژی ورزشی، دانشگاه زنجان، زنجان، ایران Seyed Ali Hosseini سید علی حسینی alihoseini_57@yahoo.com 2900319475328460042247 2900319475328460042247 No Department of Exercise Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, I.R. Iran. گروه فیزیولوژی ورزشی، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران Saeid Sedaghati سعید صداقتی sa49sd@gmail.com 2900319475328460042248 2900319475328460042248 No Department of Sport Management, Islamshahr Branch, Islamic Azad University, Islamshahr, I.R. Iran. گروه مدیریت ورزشی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران