fa ﺗﺄﺛﯿﺮ تمرین هوازی، ازون و سلول‌های بنیادی مزانشیمی بر تغییرات آستانه درد در موش‌های صحرایی مبتلا به استئوآرتریت عمومى General پژوهشي Research <div style="text-align: justify;"><span style="color:#000000;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><strong><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="letter-spacing:-.1pt">سابقه و هدف:</span></span></span></span></strong> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">استرس حاد و مزمن ناشی از</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">بی‌حرکتی به‌ویژه بی‌تحرکی </span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">ناشی از </span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">استئوآرتریت می‌تواند آستانه</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">درد و رفتارهای</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">دردی</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">را</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">متأثر کند؛</span></span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">بنابراین مطالعه حاضر با هدف </span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">تعیین </span></span><span style="background:white"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">اثر </span></span></span><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">تمرین هوازی، ازون و</span></span></span> &nbsp;<span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">MSCs</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">ب</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">ر تغییرات آستان</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt">ه</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="letter-spacing:-.1pt"> درد در <span style="background:white">رت‌های استئوآرتریتی انجام گرفت.</span></span></span></span>&nbsp;&nbsp;</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><strong><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar="">مواد</span></span></span> </strong><span lang="FA" style="font-size:9.0pt"><strong><span style="background:white"><span b="" style="font-family:" zar="">و روش‌ها:</span></span></strong><span b="" lotus="" style="font-family:"><strong> </strong>تعداد </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">45</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> سر رت نر بالغ </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">8</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">-</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">6 </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">هفته‌ای ویستار، با میانگین وزنی</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">250</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">-</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">230</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">گرم به دو گروه استئوآرتریت (</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">40</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) و کنترل سالم (</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">5</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) تقسیم شدند. استئوآرتریت از طریق جراحی در رت‌ها القا شد و سپس در </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">8</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> گروه پن<span style="background:white">ج‌</span>تایی شامل</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کنترل بیمار،</span></span> <span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">، تمرین هوازی، ازون، تمرین هوازی </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">+ </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ازون، تمرین هوازی </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">+ </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">، </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ازون + </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> و</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> تمرین هوازی</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">+ </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ازون + </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;MSCs</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">قرار گرفتند.</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تمرین هوازی به‌مدت هشت هفته و با سرعت 16متر بر دقیقه انجام شد. </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> به‌میزان </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">10⁶</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">سلول بر کیلوگرم به زانوی رت‌ها تزریق گردید؛</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">همچنین تزریق ازون در </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">3</span></span> <span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تکرار و قبل از شروع تمرین</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">با غلظت 20</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> میکروگرم بر </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">م</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">یلی‌لیتر صورت گرفت.</span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">آستان</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ه</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> درد توسط آزمون صفح</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ه</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> داغ اندازه‌گیری و سطح معنی‌داری </span><span style="background:white"><span b="" lotus="" style="font-family:">0/05 </span></span><span b="" lotus="" style="font-family:">در نظر گرفته شد.</span><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><strong><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نتایج<span style="background:white">:</span></span></span></strong> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">آستان</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ه </span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">درد </span><span style="background:white"><span b="" lotus="" style="font-family:">در گروه بیمار استئوآرتریتی در مقایسه با گروه سالم کاهش معنی‌داری داشت </span></span><span b="" lotus="" style="font-family:">(0/001</span><span new="" roman="" style="font-family:" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">)</span><span style="background:white"><span b="" lotus="" style="font-family:">.</span></span><span b="" lotus="" style="font-family:"> آستان</span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ه</span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> درد در گروه‌های </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">سلول</span></span><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">‌درمانی (</span></span><span b="" lotus="" style="font-family:">0/003</span><span new="" roman="" style="font-family:" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">)</span></span><span b="" lotus="" style="font-family:">، </span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ازو</span></span><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">ن‌درمانی </span></span><span b="" lotus="" style="font-family:">و تمرین هوازی (0/0001</span><span new="" roman="" style="font-family:" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) افزایش </span><span style="background:white"><span b="" lotus="" style="font-family:">معنی‌دار یافت</span></span><span b="" lotus="" style="font-family:">. درحالی</span><span style="background:white"><span b="" lotus="" style="font-family:">‌</span></span><span b="" lotus="" style="font-family:">که اوج این افزایش در تمرین هوازی +</span></span> <span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> دیده شد (</span></span><span lang="FA" style="font-size:9.0pt">0/02 <span new="" roman="" style="font-family:" times="">=</span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">).</span></span> <span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><span style="background:white"><strong><span b="" style="font-family:" zar="">نتیجه‌گیری:</span></strong><span b="" lotus="" style="font-family:"><strong> </strong>القای استئوآرتریت به کاهش آستان</span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ه</span></span><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:"> درد منجر می‌گردد. هرچند تمرین هوازی، ازون و</span></span></span> <span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span> <span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">با افزایش آستانه درد</span></span><span b="" lotus="" style="font-family:"> نقش مهمّی در کنترل درد حادّ </span><span style="background:white"><span b="" lotus="" style="font-family:">ناشی از استئوآرتریت</span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">دارند</span><span style="background:white"><span b="" lotus="" style="font-family:">،</span></span></span> <span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">اما </span></span></span><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">حداکثر اثر بی‌دردی در ت</span></span><span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">عامل</span></span><span b="" lotus="" style="font-family:"> تمرین هوازی + </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">MSCs</span></span> <span lang="FA" style="font-size:9.0pt"><span style="background:white"><span b="" lotus="" style="font-family:">می‌باشد.</span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><a name="_Hlk103365722"><span style="color:#000000;"><b>Background</b><b>:</b> </span></a><span style="color:#000000;">Acute and chronic stress due to inactivity, especially inactivity due to osteoarthritis can affect pain threshold and painful behaviors; therefore, this study aimed to determine the effect of </span><a name="_Hlk103363059"><span style="color:#000000;"><span lang="EN">aerobic exercise</span></span></a><span style="color:#000000;">, ozone and MSCs on pain threshold changes in osteoarthritis rats.</span></span></span></span><br> <span style="color:#000000;"><span style="font-size:12pt"><span new="" roman="" times=""><b>Materials and</b> <b>Methods:</b> Forty-five adult male Wistar rats (6-8-week-old), weighing an average of 250-230g, were divided into osteoarthritis (n=40) and healthy controls (n=5). Osteoarthritis were induced by surgery in rats and then divided into 8 groups of 5, including patient control, MSCs, <span lang="EN">aerobic </span>exercise, ozone, aerobic exercise+ozone, aerobic exercise+MSCs, ozone+MSCs and aerobic exercise+ozone+MSCs. Aerobic exercise was performed for eight weeks at a speed of 16 M/min. MSCs were injected at 1&times;10⁶ cells/kg into the knees of rats; ozone was also injected into 3 replicates before training with a concentration of 20 &mu;g/ml. Pain threshold was measured by hot-plate test and its significance level was considered 0.05.</span></span><br> <span style="font-size:12pt"><span new="" roman="" times=""><b>Results:</b> Pain threshold in the osteoarthritis patient group was significantly reduced compared to the healthy group (<i>P</i>=0.001). There was a significant increase in pain threshold in cell therapy (<i>P</i>=0.003), ozone therapy and aerobic exercise groups (<i>P</i>=0.0001). While the peak of this increase was seen in MSCs+aerobic exercise (<i>P</i>=0.02).</span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Conclusion:</b> Induction of osteoarthritis leads to a decrease in pain threshold. Although aerobic exercise, ozone and MSCs play an important role in controlling acute pain due to osteoarthritis by increasing the pain threshold, the maximum analgesic effect is in the interaction of aerobic exercise+MSCs.</span></span></span></span></span></div> تمرین, MSCs, ازون, صفحه داغ, استئوآرتریت, آستانه درد Exercise, MSCs, Ozone, Hot-Plate, Osteoarthritis, Pain threshold 247 255 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4167-1&slc_lang=fa&sid=1 Leila Abdizadeh لیلا عبدی زاده abdizadeh.ab@gmail.com 2900319475328460037634 2900319475328460037634 No Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, I.R. Iran. گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Maghsoud Peeri مقصود پیری m.peeri@iautcb.ac.ir 2900319475328460037635 2900319475328460037635 Yes Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, I.R. Iran. گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Hasan Matinhomaee حسن متین همایی hasanmatinhomaee@gmail.com 2900319475328460037636 2900319475328460037636 No Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, I.R. Iran. گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران