fa بررسی تأثیر 8 هفته محدودیت کالری بر میزان PLIN5 و ATGL بافت چرب احشایی و مقاومت به انسولین رت‌های نر مبتلا به دیابت نوع 2 عمومى General پژوهشي Research <div style="text-align: justify;"><span style="color:#000000;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">سابقه و هدف:</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ارتباط</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">نزدیکی</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">بین</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">مصرف</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">غذاهای</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">چرب،</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">همه‌گیرشدن چاقی</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">دیابت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">نوع 2</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">وجود</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">دارد. </span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">هدف از پژوهش حاضر، بررسی تأثیر 8 هفته محدودیت کالری، بر </span></span></b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">(</span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">PLIN5</span></span><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Perilipins<b> </b>5</span></span><b> </b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">ATGL</span></span><b><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> (</span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Adipose<b> </b>triglyceride<b> </b>lipase</span></span><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">) </span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">بافت چرب احشایی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و مقاومت به انسولین رت‌های نر دیابتی بود.</span></span></b><b><span lang="FA" style="font-size:9.0pt"><span style="font-family:"B Lotus""></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مواد و </span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">روش‌ها:</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">در این مطالعه تجربی 24</span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> سر موش صحرایی نر در </span></span></b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">سه</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">گروه</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کنترل</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">سالم،</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کنترل دیابتی و</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">گروه</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">دیابتی با محدودیت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کالریک</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تقسیم شدند. در گروه</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">محدودیت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کالری 40 درصد</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">از</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کالری دریافتی</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">محدود شد</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">گروه‌های</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">دیگر غذای</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">استاندارد</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">مورد‌نیاز</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">خود</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">را</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">به‌صورت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">آزادانه به‌مدت 8 هفته دریافت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کردند. </span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">بیان نسبی پروتئین </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">PLIN5</span></span><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">ATGL</span></span><b> </b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">با تکنیک وسترن‌بلات و </span></span></b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">مقاومت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">به</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">انسولین</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">نیز</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">با</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">استفاده</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">از</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">شاخص</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">هما</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">موردارزیابی</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">قرار</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">گرفت. </span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ﺩﺍﺩﻩ‌ﻫﺎ با ﺍﺳﺘﻔﺎﺩ ﺍﺯ ﺁﺯﻣﻮن ﺁﻣﺎری تحلیل واریانس </span></span></b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">یک‌طرفه</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ﻭ ﺁﺯﻣﻮﻥ تعقیبی توکی ﺁﻧﺎﻟﻴﺰ ﺷﺪﻧﺪ.</span></span></b><b> </b><b><span dir="LTR" style="font-size:9.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نتایج:</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">در مقایسه بیان پروتئین </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">PLIN5</span></span><b><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> و </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">ATGL</span></span><b><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> تفاوت معنی‌داری بین میانگین سه گروه نشان داده شد. نتایج آزمون تعقیبی بیانگر افزایش معنی‌دار</span></span></b><b> </b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">PLIN5<b> </b></span></span><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و </span></span></b><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">ATGL</span></span><b><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"> در گروه محدودیت کالری نسبت به گروه کنترل سالم و دیابتی بود (0/001</span></span></b><b><span lang="FA" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">=</span></span></b><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">). </span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">همچنین کاهش معنی‌دار انسولین و گلوکز در گروه محدودیت کالری نسبت به گروه کنترل دیابتی وجود داشت (0/001</span></span></b><b><span lang="AR-SA" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">=</span></span></b><i><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">).</span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">نتیجه‌گیری:</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">محدودیت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کالری</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">احتمالاً</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ازطریق</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تأثیر</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">بر</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">پروتئین‌های</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">پوشاننده قطرات چربی</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">و درنتیجه</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کاهش</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">تجمع</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">چربی</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">می‌تواند</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">از</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">ایجاد</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">مقاومت</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">به</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">انسولین</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">جلوگیری</span></span></b><b> </b><b><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:">کند.</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:9.0pt"><span style="font-family:"B Lotus""></span></span></b></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="color:#000000;"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background</b>: There is a close link between eating fatty foods, the prevalence of obesity and type 2 diabetes. This study aimed to evaluation the effect of 8 weeks caloric restriction on Perilipins (PLIN5) and ATGL visceral adipose tissue and insulin resistance in male diabetic rats.</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Material and Methods</b>: In this experimental study, 24 male rats were divided into healthy control, diabetic control and diabetic group with caloric restriction. In the caloric restriction group, %40 of the calorie intake was restricted and other groups received the standard food they needed freely for 8 week. The relative expression of prilipine 5 and ATGL protein by Western blotting and insulin resistance were also evaluated using Homa index. Data were analyzed by one-way ANOVA and Tukey&#39;s post hoc test.</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Result</b>: A significant difference was showed between the means of the three groups in comparing the expression of PLIN5 and ATGL proteins. Post hoc test showed a significant increase in PLIN5 and ATGL in the caloric restriction group compared to the healthy and diabetic control group (<i>P</i>=0.001). There was also a significant decrease in insulin and glucose levels in the caloric restriction group compared to the diabetic control group (p = 0.001).</span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion: </b>Calorie restriction may prevent insulin resistance by acting on the overlying proteins and thus reducing fat accumulation.</span></span></span></span></div> محدودیت کالری, پری‌لیپین 5, تری‌گلیسیرید لیپاز چربی, مقاومت به انسولین Caloric restriction, Perilipins, Adipose triglyceride lipase, Insulin resistance 47 54 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4131-1&slc_lang=fa&sid=1 Arash Alimoradi آرش علی مرادی arashalimoradi2025@gmail.com 2900319475328460039845 2900319475328460039845 No Department of Exercise Physiology٫ Faculty of Physical Education and Sport Sciences٫ Central Tehran Branch٫ Islamic Azad University٫ Tehran٫ I.R. Iran. گروه فیزیولوژی و تغذیه ورزشی، دانشکده تربیت‌بدنی و علوم ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران Hasan Mateen Homaie حسن متین همایی hasanmatinhomaee@gmail.com 2900319475328460039846 2900319475328460039846 Yes Department of Exercise Physiology٫ Faculty of Physical Education and Sport Sciences٫ Central Tehran Branch٫ Islamic Azad University٫ Tehran٫ I.R. Iran. گروه فیزیولوژی و تغذیه ورزشی، دانشکده تربیت‌بدنی و علوم ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران Saleh Rahmati صالح رحمتی احمدآباد salehrahmati@pardisiau.ac.ir 2900319475328460039847 2900319475328460039847 No Department of Exercise Physiology٫ Faculty of Physical Education and Sport Sciences٫ Central Tehran Branch٫ Islamic Azad University٫ Tehran٫ I.R. Iran. گروه فیزیولوژی و تغذیه ورزشی، دانشکده تربیت‌بدنی و علوم ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران