fa مقایسه تأثیر 8 هفته تمرین تناوبی شدید (HIIT) و تمرین استقامتی کم‌شدت (LIET) به‌همراه رژیم پرچرب بر میزان بیان ژن PPARα و PLIN5 عضله اسکلتی موش‌های صحرایی نر چاق عمومى General پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><strong><span b="" style="font-family:" zar=""><span style="color:black">سابقه و هدف:</span></span></strong><span b="" lotus="" style="font-family:"><span style="color:black"> چاقی اختلالی متابولیک با درگیری پروتئین‌های مؤثر در متابولیسم چربی می‌باشد. هدف</span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">از مطالعه حاضر، مقایسه اثرات 8 هفته تمرین تناوبی شدید (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">HIIT</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) و تمرین استقامتی کم‌شدت (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">LIET</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">) بر میزان بیان ژن‌های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PPAR&alpha;</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PLIN5</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> عضله اسکلتی موش‌های صحرایی نر دارای اضافه‌وزن</span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">بود.</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><strong>مواد و روش‌ها:</strong></span></span><span b="" lotus="" style="font-family:"><span style="color:black"> 40 سر رت نر ویستار 5هفته‌ای با</span></span></span> <span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">وزن 2/8</span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">&plusmn;</span></span><span b="" lotus="" style="font-family:"><span style="color:black">155/4 گرم به‌طور تصادفی به چهار گروه مساوی تقسیم شدند. گروه کنترل، </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">HIIT</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">LIET</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> غذای پرچرب و گروه شم غذای استاندارد به‌مدت 16 هفته مصرف کردند. تمرینات گروه </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">HIIT</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> شامل دو دقیقه با شدت 75 درصد سرعت بیشینه بود که به‌صورت تدریجی تا 90 درصد سرعت بیشینه در هفته چهارم ادامه می‌یابد. فعالیت با شدت پایین شامل دو دقیقه با شدت 30 درصد از هفته اوّل تا هفته سوم و 20 درصد سرعت بیشینه از ابتدای هفته چهارم تا انتهای دوره بود. تمرین گروه </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">LIET</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> (شامل سه قسمت گرم کردن با 30 درصد سرعت بیشینه به‌مدت 5 دقیقه، تمرین اصلی و سرد کردن با 30 درصد سرعت بیشینه به‌مدت 5 دقیقه) بود. در پایان، میزان بیان ژن‌های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PPAR&alpha;</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PLIN5</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> عضله اسکلتی به‌روش </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">Real-time pcr</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> اندازه‌گیری شد. </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><strong><span b="" style="font-family:" zar=""><span style="color:black">نتایج:</span></span></strong><span b="" lotus="" style="font-family:"><span style="color:black"> یافته‌ها نشان‌دهنده افزایش معنی‌دار بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PPAR&alpha;</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PLIN5</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> در گروه‌های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">HIIT</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">LIET</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> نسبت به گروه کنترل بوده است (0/001</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">=</span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">P</span></span></span></i><span lang="FA" style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">).</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:9.0pt"><strong><span b="" style="font-family:" zar=""><span style="color:black">نتیجه‌گیری:</span></span></strong><span b="" lotus="" style="font-family:"><span style="color:black"><strong> </strong>تمرینات</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">HIIT </span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black">&nbsp;و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">LIET</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> با افزایش بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PPAR&alpha;</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> عضله اسکلتی باعث افزایش بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" romans="" style="font-family:" time=""><span style="color:black">PLIN5</span></span></span><span style="font-size:9.0pt"><span b="" lotus="" style="font-family:"><span style="color:black"> &nbsp;قطرات چربی بافت عضلانی می‌شود. </span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="color:#000000;"><span style="font-size:12pt"><span style="line-height:106%"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Background:</b> Obesity is a metabolic disorder in which proteins involved in fat metabolism are altered. Because obesity is a growing disease among people living in today&#39;s societies, the purpose of this study was to compare the effects of 8 weeks of high-intensity intermittent training and low- intensity endurance training on PPAR&alpha; and PLIN5 gene expression in skeletal muscle of overweight rats.<span style="color:black"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:106%"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Materials and Methods:</b> Forty male Wistar rats, 5 weeks old, weighing 148.4 &plusmn;22.8 g were randomly divided into four equal groups (n=10): control, LIET, HIIT which fed high fat diet, and sham group which fed standard diet. Forty-eight hours after the end of the training program (eight weeks of intense intermittent exercise or HIIT and continued low-intensity exercise or (LIET) the rat adipose tissue was isolated under sterile conditions. Finally, expression levels of PPAR&alpha; and PLIN5 of skeletal tissue were measured by the Real-time PCR method.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:106%"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Results:</b> The data indicated that the expression level of the PPAR&alpha; and PLIN5 was significantly increased in HIIT and LIET groups compared to the control group (<i>P</i>= 0.001). </span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:106%"><span style="unicode-bidi:embed"><span new="" roman="" times=""><b>Conclusion:</b> HIIT and LIET exercises increased the expression of the PLINS gene in skeletal muscle by increasing the expression of the PPAR&alpha; gene in lipid droplets of skeletal muscle.</span></span></span></span></span></span></div> تمرین تناوبی شدید, تمرین استقامتی کم‌شدت,PPARα , PLIN5 High-intensity intermittent exercise, Low-intensity exercise, Peroxisome alpha proliferator active receptor, PLIN5 407 415 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4118-1&slc_lang=fa&sid=1 Samira Sadeghi سمیرا صادقی samirasadeghi71@yahoo.com 2900319475328460038386 2900319475328460038386 No Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, I.R. Iran. کارشناس ارشد فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران Farzaneh Taghian فرزانه تقیان F_TAGHIAN@YAHOO.COM 2900319475328460038387 2900319475328460038387 Yes Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, I.R. Iran. دانشکده علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران