Feyz Medical Sciences Journal
مجله علوم پزشکی فيض
Feyz Med Sci J
Medical Sciences
http://feyz.kaums.ac.ir
29
journal29
3060-5806
3060-5814
fa
jalali
1399
12
1
gregorian
2021
3
1
25
2
online
1
fulltext
fa
بررسی تأثیر 6 هفته تمرین استقامتی بر سطوح پروتئینهای GLUT4 و HDAC5 در عضله تند انقباض موشهای صحرایی دیابتی
Effects of six weeks endurance training on protein levels of GLUT4 and HDAC5 in EDL muscle in diabetic rats
medicine, paraclinic
medicine, paraclinic
پژوهشي
Research
<strong><span style="font-family:Zar;"><span style="font-size:9.0pt;">سابقه و هدف:</span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> ورزش و دیابت اثرات مخالفی بر پروتئین </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> دارند، اما سازوکار آن بهطور کامل در دیابت بررسی نشده است. هدف این پژوهش، بررسی اثر شش هفته تمرین استقامتی </span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">بر سطوح پروتئینهای </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>و </strong></span></span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">HDAC5</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">در عضله </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">extensor digitorum longus</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">EDL</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">) <strong>موشهای صحرایی دیابتی بود.</strong></span></span><br>
<strong><span style="font-family:Zar;"><span style="font-size:9.0pt;">مواد و روشها:</span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> تعداد 32 سر موش صحرایی نر نژاد ویستار (وزن: 4/9</span></span></strong><strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">±</span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">245 گرم) بهطور تصادفی به چهار گروه کنترل، تمرین، دیابت و دیابت - تمرین تقسیم شدند. در سنّ 8 هفتگی، دیابت بهوسیله تزریق استرپتوزوتوسین ایجاد شد. تمرین استقامتی با شدت متوسط و پیشرونده به مدت 6 هفته انجام شد. 24 ساعت پس از اتمام پروتکل، موشها تشریح شدند و بافت </span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">عضله</span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">EDL</span></span></span> <span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>استخراج گردید.</strong></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> محتوای </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> و </span></span></strong> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">HDAC5</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> بهروش الایزا اندازهگیری شد. برای تحلیل دادهها از آزمون آنالیز واریانس یکطرفه در سطح معناداری </span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">05/0></span></span></strong><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> استفاده شد. </span></span></strong><br>
<strong><span style="font-family:Zar;"><span style="font-size:9.0pt;">نتایج:</span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> نتایج نشان داد که بین گروههای کنترل و دیابت (0005/0</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">)، دیابت - تمرین و دیابت (0005/0</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">) و تمرین و دیابت (0005/0</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">) تفاوت معناداری در سطوح </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> وجود دارد. همچنین، نتایج نشان داد که بین گروههای کنترل و تمرین (138/0</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">)، دیابت - تمرین و دیابت (229/0=</span></span></strong> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">) و تمرین و دیابت (306/0</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">) تفاوت معناداری در سطوح پروتئین </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">HDAC5</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>وجود ندارد و بین بقیه گروهها تفاوت معناداری وجود دارد (05/0</strong></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">></span></span></strong><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">). همچنین، رابطه معکوس غیرمعناداری بین سطوح پروتئینهای </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>و</strong></span> </span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">HDAC</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> وجود دارد (354/0</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> و 169/0-</span></span></strong><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">r</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">).</span></span></strong><br>
<strong><span style="font-family:Zar;"><span style="font-size:9.0pt;">نتیجهگیری:</span></span></strong><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> اثر مثبت تمرین استقامتی بر سطوح پروتئین </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>در گروه دیابتی بیشتر از گروه سالم بود و با اینکه تمرین استقامتی بر روی سطوح پروتئینهای </strong></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>و </strong></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">HDAC5</span></span></span><strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> اثر مثبت داشت</span></span></strong> <strong><span style="font-family:B Lotus;"><span style="font-size:9.0pt;">و اثر بر پروتئین </span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:9.0pt;">GLUT4</span></span></span><span style="font-family:B Lotus;"><span style="font-size:9.0pt;"> <strong>بیشتر و معنادارتر بود.</strong></span></span>
<span style="font-family:Times New Roman;"><strong>Background:</strong> Exercise and diabetes have opposite effects on GLUT4 protein, but its mechanism has not been fully investigated. This study aimed to investigate the effect of six weeks of endurance training on protein levels of GLUT4 and HDAC5 in extensor digitorum longus (EDL) muscle in diabetic rats.<br>
<strong>Materials and Methods:</strong> 32 male Wistar rats (weight: 245±9.4 g) were randomly divided into 4 groups: Control, exercise, diabetic, diabetic-exercise. At 8 weeks of age, diabetes was induced by streptozotocin. The endurance training was performed for 6 weeks. 24 hours after the last session, the mice were sacrificed and EDL muscle was extracted. The protein levels of GLUT4 and HDAC5 were measured using ELISA method. One-way ANOVA was used to analyze the data at a significant level of <em>P</em><0.05.<br>
<strong>Results:</strong> Results showed significant differences in GLUT4 levels between control and diabetic (<em>P</em>=0.0005), diabetic-exercise and diabetic (<em>P</em>=0.0005) and diabetic and exercise groups (<em>P</em>=0.0005). Also, the results showed that there was no significant difference in HDAC5 protein levels between control and exercise (<em>P</em>=0.138), diabetic-exercise and diabetic (<em>P</em>=0.229) and exercise and diabetic groups (<em>P</em>=0.306), and there was a significant difference between the other groups (<em>P</em><0.05). Also, There was a non-significant inverse relationship between GLUT4 and HDAC5 protein levels (r=-0.169, <em>P</em>=0.354).<br>
<strong>Conclusion:</strong> The positive effect of endurance training on GLUT4 protein levels was greater in the diabetic than in the healthy group. Although endurance training had a positive effect on GLUT4 and HDAC5 levels and effect on GLUT4 protein was greater and more significant.</span>
دیابت, گلوکز, تمرین استقامتی, GLUT4, HDAC5
Diabetes, Glucose, Endurance Training, GLUT4, HDAC5
830
838
http://feyz.kaums.ac.ir/browse.php?a_code=A-10-3224-4&slc_lang=fa&sid=1
Mohammad sharif
Bagheri
محمدشریف
باقری
vahidvalipourdehnou@gmail.com
2900319475328460033821
2900319475328460033821
No
Vahid
Valipour dehnou
وحید
ولی پور ده نو
valipour.v@lu.ac.ir
2900319475328460033822
2900319475328460033822
Yes
Ahmad
Hematfar
احمد
همت فر
valipour114@yahoo.com
2900319475328460033823
2900319475328460033823
No