Feyz Medical Sciences Journal
مجله علوم پزشکی فيض
Feyz Med Sci J
Medical Sciences
http://feyz.kaums.ac.ir
29
journal29
3060-5806
3060-5814
fa
jalali
1398
12
1
gregorian
2020
3
1
24
1
online
1
fulltext
fa
بررسی اثر سافرانال بر میزان Bax ،Bcl2 و شاخص های استرس اکسیداتیو در بافت بیضه موش های صحرایی دیابتی شده با استرپتوزوتوسین
Investigating the effect of safranal on Bax and Bcl2 and oxidative stress levels in testis tissue of streptozotocin-induced diabetic rats
medicine, paraclinic
medicine, paraclinic
پژوهشي
Research
<div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">سابقه و هدف:</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">یکی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">از</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">شایع</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ترین</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بیماری</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">غدد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">درون</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ریز، دیابت نوع یک</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">می</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">باشد که </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">استرس ­های اکسیداتیو را در بافت بیضه ایجاد می­ کند.</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">امروزه برای</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">درمان</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">دیابت،</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">گرایش</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سوی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">داروهای</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">گیاهی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">روز­به ­روز افزایش</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">می</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">یابد.</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">این</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">هدف</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بررسی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سافرانال</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بر شاخص ­های آنتی­ اکسیدانت و </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">میزان </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bax</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> و</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bcl2 </span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">موش</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">صحرایی انجام شد.</span></span></strong><br>
<strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">مواد و روش ­ها:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">این</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تجربی،</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">24</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">موش</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">صحرایی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به چهار</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">گروه </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">شاهد، شاهد دیابتی و­­ 2 گروه دیابتی تحت تیمار با سافرانال (50 و 100 میلی­ گرم بر میلی ­لیتر، 25 روز تزریق داخلصفاقی) تقسیم شدند.</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> گروه­ های دیابتی با یکبار تزریق داخلصفاقی استرپتوزوتوسین </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با غلظت 55 میلی­ گرم بر کیلوگرم وزن بدن موش</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> دیابتی شدند. نتایج توسط نرمافزار آماری </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">SPSS</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ویرایش 20 و آزمون­ های­ </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">آنالیز واریانس چند متغیره </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">(</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MANOVA</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">)</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> و آزمون ­های اثرات بینگروهی </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> (Tests of Between-Subjects Effects)</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و روش داده ­های تکرارشده </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">(Repeated Measure)</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تحلیل شدند.</span></span></strong><br>
<strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتایج:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> سطح بافتی شاخص </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bax</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و مقدار مالون­ دی­ آلدئید در گروه تحت درمان با غلظت</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">mg/ml </span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">100 سافرانال در مقایسه با گروه تحت درمان با غلظت </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">mg/ml</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">50 سافرانال و گروه شاهد دیابتی کاهش معنی­ داری داشت. در مقدار سطح بافتی شاخص </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bcl2</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> و آنزیم ­های گلوتاتیون پراکسیداز، سوپر اکسید­ دیسموتاز و کاتالاز در گروه تحت درمان با غلظت </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">mg/ml</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">100 سافرانال نسبت به گروه تحت درمان با غلظت</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> mg/ml </span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">50 سافرانال و گروه شاهد دیابتی افزایش معنی­ داری مشاهده شد </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">(0/05></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) که نشاندهنده اثر وابسته به دوز سافرانال می ­باشد. همچنین سافرانال موجب کاهش معنی­ داری در میزان قند خون موش­ های دیابتی شد.</span></span></strong><br>
<strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتیجه ‏گیری:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> سافرانال میتواند موجب بهبود شاخص ­های آنتی­ اکسیدانی شود و آسیب­ های القاشده توسط دیابت­ در بافت بیضه موش­ های دیابتی را کاهش دهد. </span></span></strong></div>
<div style="text-align: justify;"><span style="font-family:Times New Roman;"><strong>Background: </strong>One of the most common endocrine diseases is type 1 diabetes, which causes oxidative stress in testis tissue. Today, in the treatment of diabetes, the tendency toward herbal medicines is increasing day by day. This study aimed to investigate the effects of safranal on Bax and Bcl2 and anti-oxidant levels of streptozotocin-induced diabetic rats.<br>
<strong>Materials and Methods:</strong> In this experimental study, 24 rats were divided into 4 groups: control, non-treated diabetic and 2 safranal-treated diabetic groups (50, 100 mg/ml, 25 days intraperitoneal injection). The diabetic groups were diabetic with intraperitoneal injection of streptozotocin (STZ) at a concentration of 55 mg/kg body weight of the mouse. On day 25, the testicles were exited to evaluate antioxidant enzymes and Bax and Bcl2. The results were analyzed by using SPSS 20 software, one-way ANOVA and LSD tests<br>
<strong>Results: </strong>The level of pro-apoptotic Bax and malondialdehyde level in the treated group with a concentration of 100 mg/ml of safranal was significantly reduced compared to the treatment group with a concentration of 50 mg/ml of safranal and control group. However, the level of pro-apoptotic Bcl-2 and glutathione peroxidase, superoxide dismutase and catalase enzymes in the treated group with a concentration of 100 mg/ml of safranal was significantly increased compared to the treatment group with a concentration of 50 mg/ml of safranal and control group (<em>P</em><0.05); which indicates the effect of safranal concentration. Also, safranal significantly reduced the level of glucose in diabetic rats.<br>
<strong>Conclusion:</strong> Safranal can improve the antioxidant activity and decrease the damage induced by diabetes in the testis tissue of diabetic rats.</span></div>
سافرانال, Bax, Bcl2, بیضه, موش صحرایی, دیابت
Safranal, Bax protein, Bcl2 proteins, Testicle, Rat, Diabetes mellitus
10
20
http://feyz.kaums.ac.ir/browse.php?a_code=A-10-3206-1&slc_lang=fa&sid=1
Ghazal
Ataei
غزال
عطائی
gh72ataei@gmail.com
2900319475328460029492
2900319475328460029492
No
Department of Biology, Faculty of Sciences, Payam Noor University, Tehran, I.R. Iran.
گروه زیست شناسی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران
Raheleh
Rahbarian
راهله
رهباریان
ra_rahbarian@yahoo.com
2900319475328460029493
2900319475328460029493
Yes
Department of Biology, Faculty of Sciences, Payam Noor University, Tehran, I.R. Iran.
گروه زیست شناسی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران