fa بررسی میزان بیان ژن P53 در سلول‎های همسایه بعد از تابش امواج فراصوت درمانی medicine, paraclinic medicine, paraclinic پژوهشي Research <div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">سابقه و هدف: </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">اثر همسایگی پرتویی، بروز آثار بیولوژیکی در سلول</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">&lrm;</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">هایی است که به&shy; طور مستقیم تحت تابش پرتو قرار نگرفته</span></span></strong><strong><span dir="LTR"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">اند؛ بلکه آثار پرتوی، از طریق پیام</span></span></strong><strong><span dir="LTR"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های صادره از سلول</span></span></strong><strong><span dir="LTR"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های تابش</span></span></strong><strong><span dir="LTR"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">دیده در آن&shy;ها القا می&lrm;شود.</span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> یکی از روش</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های غیرتهاجمی درمان سرطان، استفاده از امواج فراصوت می</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">باشد. بازده درمانی این روش همانند درمان با پرتوهای یونیزان می</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تواند تحت تأثیر اثر همسایگی تغییر کند</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">که این مطلب لزوم بررسی اثر همسایگی ناشی از امواج فراصوت را می</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">رساند. در</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">این</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">پژوهش</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تأثیر تابش</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">امواج</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">فراصوت</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">درمانی بر بیان ژن </span></span></span></strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P53</span></span></span></span><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>در سلول</strong></span></span></span><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های همسایه مورد بررسی قرار گرفته &shy;است.</span></span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">مواد و روش</span></span></strong><strong><span dir="LTR"><span style="font-family:b zar;"><span style="font-size:9.0pt;">&lrm;</span></span></span></strong><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">ها:</span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ها به سه گروه کنترل (بدون درمان)، هدف (سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های در معرض تابش امواج فراصوت) و همسایه (سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های تابش</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times,serif;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ندیده و دریافت</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times,serif;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">کننده محیط کشت سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های هدف)</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تقسیم شدند. سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های گروه هدف، تحت تابش فراصوت قرار گرفتند و پس از یک ساعت محیط کشت آن</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ها به سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های گروه همسایه منتقل شد. میزان بقای سلولی و همچنین میزان بیان ژن </span></span></span></strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P53</span></span></span></span><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به &shy;ترتیب بااستفاده از تست </span></span></span></strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MTT</span></span></span></span><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> و </span></span></span></strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Real-time PCR</span></span></span></span> <span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&nbsp;<strong>بررسی شد.</strong></span></span></span><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتایج: </span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">درصد</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بقای</span></span></span></strong> <strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سلولی گروه هدف در مقایسه با گروه کنترل کاهش یافت، اما در گروه همسایه این تفاوت معنادار نبود. میزان بیان ژن </span></span></span></strong><span dir="LTR"><span style="color:black;"><span style="font-family:times,serif;"><span style="font-size:9.0pt;">P53</span></span></span></span><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>در گروه</strong></span></span></span><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> همسایه در مقایسه با گروه کنترل افزایش معناداری نشان داد.</span></span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتیجه&shy; گیری: </span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">اثر همسایگی به&shy; دنبال تابش فراصوت ایجاد شد و آن می‌تواند میزان بیان ژن </span></span></span></strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P53</span></span></span></span><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> را در سلول</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">های همسایه افزایش </span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">&lrm;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;">دهد.</span></span></span></strong></div> <div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background:</strong> Bystander effect is defined as biological responses observed in cells that are not exposed to radiation directly but these radiation effects are induced through messages from irradiated cells. One of the non-invasive methods for cancer treatment is ultrasound waves. The therapeutic efficacy of ultrasound waveslike treatment with ionizing radiation can be altered by the bystander effect, so it is necessary to evaluate the bystander effect after ultrasound exposure. In this study, the effect of ultrasound exposure on the expression of P53 gene in neighboring cells has been investigated.<br> <strong>Materials and Methods:</strong> The cells were divided into three groups: control (without treatment), target (under direct ultrasound exposure) and the bystander (no radiation, only receiving target cells culture media). In the target group, the cells were exposed to the ultrasound and after one hour their culture medium were transferred to the bystander group. Cell viability and the expression of P53 gene by using the MTT assay and Real-time PCR was analyzed.<br> <strong>Results:</strong> The percentage of cell survival in the target group was reduced compared to the control group, but in the bystander group, this difference was not significant. The expression of P53 gene in the bystander group was statistically increased compared to the control group.<br> <strong>Conclusion:</strong> The bystander effect is induced after ultrasound exposure and it can increase the level of P53 gene expression in bystander cells.</span></div> <div style="text-align: justify;"></div> امواج فراصوت, اثر همسایگی, تست MTT,Real-time PCR, ژن P53 Ultrasound waves, Bystander effect, MTT assay, Real-time PCR, P53 gene 344 351 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-2765-1&slc_lang=fa&sid=1 Masumeh Rezaei معصومه رضایی masumrezaei@yahoo.com 2900319475328460026657 2900319475328460026657 No Department of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. گروه فیزیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران Roghayeh Kamran-Samani رقیه کامران سامانی kamran.samani65@gmail.com 2900319475328460026658 2900319475328460026658 No Department of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. گروه فیزیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران Ahmad Shanei احمد شانئی a.shanei1397@gmail.com 2900319475328460026659 2900319475328460026659 Yes Department of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. گروه فیزیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران Mohammad Kazemi محمد کاظمی m_kazemi@med.mui.ac.ir 2900319475328460026660 2900319475328460026660 No Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. گروه ژنتیک، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران Seyed Hossein Hejazi سید حسین حجازی hejazi@med.mui.ac.ir 2900319475328460026661 2900319475328460026661 No Department of Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. گروه قارچ شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران