fa بررسی میزان بیان ژن های PTEN و AKT1 در کشت هم جوار سلول‌های سرطانی کولون HT29 با باکتری استرپتوکوکوس ترموفیلوس medicine, paraclinic medicine, paraclinic پژوهشي Research <div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">سابقه</span></span></strong> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">و</span></span></strong> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">هدف:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> سرطان کولون از سرطان&shy; های رایج در جهان می&shy;باشد. </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">پروبیوتیک‌ها میکروارگانیسم‌های زنده</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مفیدی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">هستند</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">که با</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تأثیر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">آنزیم‌های گوارشی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">حیوانات</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">انسان&shy; ها،</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مهار</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">عوامل</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سرطان &shy;زا</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">داخل</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بدن</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و شرایط</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">آزمایشگاهی،</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سرکوب</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">لوسیون‌ها و</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ترکیبات</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">القا&shy;کننده سرطان</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تومورها</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">حیوانات</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">آزمایشگاهی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">نقش</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مؤثری</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در جهت</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مقابله</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سرطان</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ایفا می‌کنند. هدف مطالعه حاضر بررسی مسیر سیگنالینگ سلولی </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PTEN/AKT1</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در کشت همجوار باکتری <em>استرپتوکوکوس ترموفیلوس</em> با سلول&shy; های سرطانی کولون </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">HT29</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> می &shy;باشد.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">مواد روش&shy; ها:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> از</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">کشت</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">باکتری&shy; ها،</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مایع</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">رویی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">عصاره</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">باکتریایی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تهیه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">شده</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سلول‌های سرطانی کولون</span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">HT29</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">توسط</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">این</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مواد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تیمار شدند. نکروز</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سلولی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با روش</span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MTT</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بررسی گردید. همچنین، میزان بیان ژن&shy; های</span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PTEN </span></span></span></em>&nbsp;<span style="font-family:b lotus;"><span style="font-size:9.0pt;">و </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">AKT1</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در رده سلولی </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&nbsp;HT29</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با استفاده از روش </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Real Time PCR</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> مورد بررسی قرار گرفت.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتایج:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> باکتری‌</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <em>استرپتوکوکوس ترموفیلوس</em> </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">میزان بیان ژن </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">AKT1</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> را کاهش داده، بیان </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PTEN</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> را </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">افزایش &shy;داده </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و سلول&shy; های سرطانی را به سمت آپوپتوز &shy;برد</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">.</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> آزمایش </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MTT</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">نشان داد که غلظت 0/05</span></span></strong><strong><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">=</span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">OD</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بیشترین کشندگی طی 4 ساعت را دار</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">د.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتیجه&shy; گیری:</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">می &shy;توان از باکتری‌</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <em>استرپتوکوکوس ترموفیلوس</em></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> جهت ایجاد یک راه&shy; کار نوین درمانی با تأثیر بالا، عوارض جانبی پایین، بی&shy; خطر از نظر بیولوژیکی و هزینه کمتر و یا به &shy;عنوان درمان جانبی متناسب با فلور طبیعی بدن برای درمان و همچنین پیشگیری از سرطان کولون بهره برد</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">. </span></span></strong></div> <div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background:</strong> Colon cancer is one of the most common cancers in the world. Probiotics are viable and useful microorganisms that have an effective role in controlling cancer by influencing the digestive enzymes of animals and humans, inhibition of cancerous agents in the body and in vitro conditions, suppression of lotions, and cancer-inducing compounds and tumors in experimental animals. The present study was conducted to investigate the <em>PTEN/ AKT1</em> cellular signaling pathway in adjacent cultures of <em>Streptococcus thermophil</em><em>us</em> with HT29 colon cancer cells.<br> <strong>Materials and Methods:</strong> Bacterial culture, supernatant and bacterial extract were prepared and the cells were treated with these materials. Cell necrosis was evaluated by the MTT method. Also, the expression of <em>PTEN </em>and <em>AKT1</em> genes in HT29 cell line was investigated using the real-time PCR.<br> <strong>Results:</strong> The results showed that the thermophiles bacterium reduced the expression of <em>AKT1</em> genes, and increased the expression of <em>PTEN </em>and led the cancerous cells toward apoptosis. The MTT test showed that the concentration of OD=0.05 had the highest mortality in 4 hours.<br> <strong>Conclusion:</strong> Thermophiles bacteria can be used to create a novel therapeutic effect with high impact, low side effects, harmless biological effect and lower costs. Also, it can be used as an extra treatment appropriate to the body&#39;s natural flora for the treatment and prevention of colon cancer.</span></div> پروبیوتیک, سرطان کولون, سلول HT29 Probiotic, Colon cancer, HT29 cell 624 631 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-2562-1&slc_lang=fa&sid=1 ُShiva Sattari شیوا ستاری shivasattari57@gmail.com 2900319475328460024027 2900319475328460024027 No Department of Biology, Bonab Branch, Islamic Azad University, Bonab, I. R. Iran. گروه زیست شناسی، واحد بناب، دانشگاه آزاد اسلامی بناب، ایران Changiz Ahmadizadeh چنگیز احمدی زاده dr_ahmadizadeh@yahoo.com 2900319475328460024028 2900319475328460024028 Yes Department of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, I. R. Iran. گروه میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی اهر، ایران