fa بررسی نسبت بیان ژن های Bcl2/BAX به عنوان یک علامت بیماری التهابی روده (IBD) عمومى General پژوهشي Research <div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">سابقه و هدف:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> آپوپتوز یک فرآیند فیزیولوژیک ضروری در حفظ هموستازی ایمنی است.</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به &shy;نظر می &shy;رسد </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">آپوپتوز ناصحیح سلول&shy; های </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">T</span></span></span><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>در بیماری&shy; های التهابی مزمن، همچون بیماری التهابی روده (</strong></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IBD</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) دخالت داشته باشد. هدف از این مطالعه ارزیابی نسبت بیان ژن&shy; های </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bcl2</span></span></span></em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>به </strong></span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">BAX</span></span></span></em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>در مبتلایان به </strong></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IBD</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> (بیماری کرون و کولیت زخم&shy; شونده) نسبت به افراد سالم بود.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">مواد و روش &shy;ها:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">این</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">موردی</span></span></strong><strong><span dir="LTR"><span style="font-family:bmitra;"><span style="font-size:9.0pt;">-</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> شاهدی، 49</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بیمار</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مبتلا</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به </span></span></strong><span dir="LTR"><span style="font-family:timesnewromanpsmt,serif;"><span style="font-size:9.0pt;">&nbsp;IBD</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">20</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">فرد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سالم</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مورد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بررسی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">قرار</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">گرفتند</span></span></strong><strong><span dir="LTR"><span style="font-family:bmitra;"><span style="font-size:9.0pt;">.</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> خونگیری از بیماران مبتلا به</span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IBD</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و افراد سالم پس از دریافت کامل اطلاعات و کسب رضایت انجام و</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> سپس سلول&shy; های سفید خون به &shy;روش سالتینگ اوت جداسازی شد. بعد از استخراج </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">RNA</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> و سنتز </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">cDNA</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">، سطح بیان ژن&shy; های </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bcl2</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">BAX</span></span></span></em> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به&shy; صورت کمّی به&shy;روش </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">real-time PCR</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در افراد سالم و بیمار اندازه &shy;گیری شد.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتایج:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در این مطالعه، آنالیز </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">real-time PCR</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> نشان داد که نسبت بیان ژن&shy; های </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bcl2</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">BAX</span></span></span></em><em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>در </strong></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سلول&shy; های </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">T</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> خون محیطی در 49 فرد مبتلا به </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IBD</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در مقایسه با 20 فرد سالم&nbsp; تفاوت معنی&shy; داری نداشت (</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">0/06</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"> =</span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">). </span></span></strong><br> <strong><span style="background:white;"><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتیجه&shy; گیری:</span></span></span></strong><strong><span style="background:white;"><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به&shy; نظر می &shy;رسد در </span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">افراد مبتلا به </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IBD</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">، تغییر در&nbsp; نسبت بیان ژن &shy;های </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Bcl2</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">BAX</span></span></span></em><strong><em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در </span></span></em></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">سلول&shy; های </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">T</span></span></span><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>خون محیطی مشهود نباشد.</strong></span></span></div> <div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background</strong><strong>:</strong> Apoptosis is an essential physiological process to maintain immune homeostasis. T-cell incorrect apoptosis appears to be involved in chronic inflammatory diseases such as inflammatory bowel disease (IBD). The aim of this study was to evaluate the <em>Bcl2</em>/<em>BAX</em> genes expression ratio in patients with IBD (Crohn&rsquo;s disease and ulcerative colitis) compared to healthy persons.<br> <strong>Materials and </strong><strong>Methods:</strong> This case-control study was conducted on 49 patients with IBD and 20 healthy subjects. Blood samples were collected from the patients and healthy subjects after receiving complete information and obtaining consent. Then, white blood cells were isolated by the Salting Out technique. After RNA extraction and cDNA synthesis, the <em>Bcl2</em>/<em>BAX</em> expression level was quantitatively measured by the real-time PCR method in the patients and healthy subjects.<br> <strong>Results:</strong> The results showed that the <em>Bcl2</em>/<em>BAX</em> genes expression ratio in peripheral blood T-cells was not significantly different in patients with IBD compared to the healthy subjects (<em>P</em>=0.06).<br> <strong>Conclusion:</strong> It seems that change is not evident in <em>Bcl2/BAX</em> genes expression ratio in peripheral blood T-cells in the patients with IBD.</span></div> آپوپتوز, BAX ,Bcl2, بیماری التهابی روده Apoptosis, BAX, Bcl2, Inflammatory bowel disease 153 157 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-2502-1&slc_lang=fa&sid=1 Fariba Javadinia فریبا جوادی نیا faribajavadi@ymail.com 2900319475328460025322 2900319475328460025322 No Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, I. R. Iran. گروه زیست شناسی سلولی و مولکولی، دانشکده علوم زیستی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Zeinab Khazaei Koohpar زینب خزائی کوهپر dz.khazaei@gmail.com 2900319475328460025323 2900319475328460025323 Yes Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, I. R. Iran. گروه زیست شناسی سلولی و مولکولی، دانشکده علوم زیستی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Hamid Asadzadeh Aghdaei حمید اسدزاده عقدایی hamid.assadzadeh@gmail.com 2900319475328460025324 2900319475328460025324 No Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, I. R. Iran. مرکز تحقیقات علوم پایه و اپیدمیولوژی بیماری های دستگاه گوارش، پژوهشکده بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران