fa مطالعه ارتباط بین چندشکلی ژن‌های (A>G rs3742330) Dicer و (rs10719 C>T) Drosha با خطر سقط مکرر medicine, paraclinic medicine, paraclinic پژوهشي Research <div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">سابقه و هدف:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> مولکول‌های </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Dicer</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> و</span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Drosha</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به &shy;عنوان تنظیم&shy; کننده‌های مهم در بیوژنز </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">miRNA<strong>‌</strong></span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ها نقش دارند. چندشکلی در ژن‌‌های مذکور ‌می &shy;تواند باعث نقص در فرآیند لانه &shy;گزینی جنین و منجر به سقط شود. هدف از این مطالعه ارزیابی </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ارتباط </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بین چندشکلی </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ژن‌های (</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">A>G</span></span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs 3742330</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Dicer</span></span></span></em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>و (</strong></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs 10719 C>T</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Drosha</span></span></span></em> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با خطر سقط‌ مکرر بود.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">مواد</span></span></strong><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;"> و روش‌ها:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> مطالعه مورد-شاهدی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">حاضر روی 100 خانم مبتلا به سقط مکرر با عامل ناشناخته و 100 خانم بدون تاریخچه سقط و واجد یک فرزند زنده مراجعه‌ کننده به بیمارستان امام خمینی اردبیل طی سال‌های 1396-1395 انجام شد. با روش </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PCR-RFLP</span></span></span><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>فراوانی </strong></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">چندشکلی </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ژن‌های (</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">A>G</span></span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs 3742330</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Dicer</span></span></span></em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>و (</strong></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs 10719 C>T</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Drosha</span></span></span></em> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">مورد بررسی </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">قرار گرفت.</span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتایج:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> بین فراوانی ژنوتیپی چندشکلی </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Dicer</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در افراد سالم و بیمار از نظر آماری اختلاف معنی&shy; داری وجود نداشت (0/05</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">></span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">). از طرف دیگر، بین فراوانی ژنوتیپی چندشکلی </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Drosha</span></span></span></em> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در افراد سالم و بیمار اختلاف معنی&shy; دار دیده شد (0/05</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;"><</span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">). </span></span></strong><br> <strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتیجه&shy; گیری: </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به نظر می‌رسد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">چندشکلی </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs 10719</span></span></span> <em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Drosha</span></span></span></em><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>عامل مستعد کننده</strong></span></span><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> ژنتیکی برای سقط‌ مکرر باشد، این در حالی است که چندشکلی ژن </span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Dicer</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> به&shy; عنوان عامل خطر سقط مکرر در جمعیت مورد مطالعه نقشی ندارد.</span></span></strong></div> <div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background:</strong> Drosha and Dicer are important molecules that play critical regulatory roles in the biogenesis of micro-RNA. Genetic polymorphism in the <em>Drosha</em> and <em>Dicer</em> can cause defect on the embryo implantation and lead to the recurrent abortion. The aim of this study was to evaluate an association between <em>Drosha</em> and <em>Dicer</em> gene polymorphisms and the risk of recurrent pregnancy loss (RPL).<br> <strong>Materials and Methods:</strong> This case-control study was performed on 100 women with RPL (with unknown reasons) and 100 women with a successful pregnancy (one alive child) and no abortion history referred to Imam Khomeini Hospital in Ardebil city during 2015-2017. The frequencies of these polymorphisms were evaluated using the PCR-RFLP method.<br> <strong>Results:</strong> Results showed no statistically significant difference in the genotype frequency of the <em>Dicer </em>gene polymorphism between the case and control groups (<em>P</em>>0.05). On the other hand, a statistically significant difference was found in the genotype frequency of the <em>Drosha</em> gene polymorphism between the groups (<em>P</em><0.05).<br> <strong>Conclusion:</strong> It seems that the <em>Drosha</em>&nbsp;gene polymorphism can be a predisposing genetic factor for RPL, whereas the <em>Dicer</em> gene polymorphism cannot be considered as a risk factor for predisposing RPL in the studied population.</span></div> سقط مکرر, چند شکلی, Drosha ,Dicer Recurrent abortion, Polymorphism, Drosha, Dicer 169 176 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-2332-1&slc_lang=fa&sid=1 Negin Mohseni نگین محسنی negin_n137027@yahoo.com 2900319475328460021055 2900319475328460021055 No Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, I. R. Iran. گروه آموزشی ژنتیک مولکولی، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران Saeid Ghorbian سعید قربیان ghorbian20@yahoo.com 2900319475328460021056 2900319475328460021056 Yes Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, I. R. Iran. گروه آموزشی ژنتیک مولکولی، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران