TY - JOUR JF - KAUMS JO - Feyz VL - 26 IS - 6 PY - 2022 Y1 - 2022/12/01 TI - The effect of high-fat diet and continuous endurance training on expression of TFEB and E2F1 transcription factors in visceral adipose tissue of mice TT - تأثیر تغذیه پرچرب و تمرین استقامتی تداومی بر بیان فاکتورهای رونویسی TFEB و E2F1 در بافت چربی احشائی موش‌های سوری N2 - Background: Transcription Factor EB (TFEB) and Transcription Factor E2F1 play an important role in metabolism. This study investigated the effect of exercise training and high-fat diet on the gene expression of these factors in the visceral adipose tissue of mice. Materials and Methods: In the experimental study, 28 male mice (C57BL/6) were assigned into four groups included Control, High-Fat Diet (HFD), Exercise Training (ET), and High-Fat Diet with Exercise Training (HFD-ET). The subjects of HFD were fed a high-fat diet for 12 weeks. The mice of ET performed continuous endurance training on a treadmill for six weeks. The mice of HFD-ET had six weeks of endurance training in addition to having the HFD. The Real-Time–PCR methods were used to measure the gene expression of TFEB and E2F1. Results: 1) The gene expression of TFEB was increased by HFD and ET (P<0.05). 2) The combination of HFD and ET had an increasing effect on TFEB (P=0.02); However, this effect was not higher than ET and HFD, separately (P>0.05). 3) HFD caused an increase in E2F1 (P=0.03). 4) Neither ET nor combined HFD and ET significantly increased the expression of E2F1 (P>0.05). Conclusion: Continuous endurance training has a similar effect (no opposite effect) to the high-fat diet on gene expression of TFEB. Further, it could be thought that this exercise training may partly negate the increasing effect of the high-fat diet on E2F1 expression. SP - 646 EP - 656 AU - Daneshyar, Saeed AU - Mirakhori, Zahra AU - Forutan, Yazdan AD - KW - High-fat diet KW - Exercise training KW - Endurance training KW - Adipose tissue KW - TFEB KW - E2F1 UR - http://feyz.kaums.ac.ir/article-1-4677-en.html DO - 10.48307/FMSJ.2022.26.6.646 ER -