TY - JOUR JF - KAUMS JO - Feyz VL - 21 IS - 4 PY - 2017 Y1 - 2017/10/01 TI - Prevalence of metallo-beta-lactamase enzyme and patern of antibiotic resistance in Klebsiella pnomoniae isolated from patients with urinary tract infection in Qom city during 2013-2014 TT - بررسی فراوانی آنزیم متالوبتالاکتاماز و الگوی مقاومت آنتی بیوتیکی در سویه های کلبسیلا پنومونیه جدا شده از بیماران مبتلا به عفونت ادراری در شهر قم طی سال های 1392 تا 1393 N2 - Bachground: Klebsiella pnomoniae is one of the most important etiologic agents of urinary tract infection (UTI). An increasing occurrence of antimicrobial resistance among uropathogenic bacterial isolates has complicated the treatment process. The aim of this study was to determine antibiotic susceptibility patterns and prevalence of the metallo-beta-lactamase enzyme of K. pneumoniae isolates collected from UTI. Materials and Methods: This cross-sectional study was conducted on patients with complicated UTI reffered to hospitals in Qom city, Iran. A total of 1807 culture positive samples of pathogens were collected from the patients, among which 457 isolates were K. pneumoniae. The isolates were tested for antimicrobial susceptibility by the disc-diffusion method recommended by the guidelines of Clinical and Laboratory Standards Institute (CLSI 2013). In addition, the dubble disk synergy test was used to detect the K. pneumoniae isolates of metallo-beta-lactamase enzyme. Results: The prevalence of UTI infection due to K. pneumoniae was 25.3%. Among 1807 positive urine cultures, 62.4% were from females and 37.6% from males. Results of antimicrobial susceptibility showed that the highest antibiotic resistance was seen for trimetoprium-sulfametoxazole (98.5%) and the lowest resistance levels were seen for amikacin (9.4%), meropenem (22.8%) and imipenem (25.6%). The results of the imipenem-EDTA combined disk showed that 93.2% imipenem resistance isolates were positive for the metallo-beta- lactamase enzyme. Conclusion: Carbapenem resistance and production of the metallo-beta-lactamase enzyme in K. pneumoniae uropathogenic are increasing. However, amikacin is still effective against these bacterial infections and its effectiveness should be maintained. SP - 390 EP - 397 AU - Khodadadian, Rohollah AU - Khorshidi, Ahmad AU - Safari, Mahmoud AU - Gilasi, Hamid Reza AD - Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R. Iran. KW - Klebsiella pnomoniae KW - UTI KW - Antibiotic resistance KW - Metallo-Beta-Lactamases UR - http://feyz.kaums.ac.ir/article-1-2962-en.html ER -