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Showing 9 results for Rectal
Ziba Mosayebi, Rezvan Moniri, Ali Reza Eslami, Elahe Mesdaghinia, Sayed Gholam Abbas Mousavi,
Volume 5, Issue 4 (1-2002)
Abstract
History and Objectives: Considering the various report on incidence of Streptococcus type-B in rectum and its role in premature infantile infection and lack of information in the region and for determination of its prevalence in pregnant women, this study was carried out in Shabihkhani hospital in Kashan in 2000. Materials and Methods: The descriptive strategy of this study was conducted on 400 pregnant women at a gestational age greater than 35 weeks. For this purpose, a questionnaire was designed for collection of data including age, occupation, academic history, nationality, inhabitation, multiparous state, number of abortion, labor type and antibiotic consumption. Sampling from rectum was done using a sterile swab. Then, it was cultured in special medium and isolated bacteria were identified using biochemical tests. All of the infants up to 48h after labor and those from mothers with signs of positive colonization up to one week were followed up. From the latter group, sampling was done and the related bacteria were identified. For statistical analysis, X² and Fischer tests were used. Results: Out of 400 samples from rectum, 30 cases (7.5%) with streptococcus type-B and in 2 cases (6.7%) from mothers with positive culture were identified. In addition there was a relationship between streptococcus type-B rectal colonization and premature infantile premature infection (P=0.0055). Conclusion and Recommendations: Considering a 7.5% incidence of this bacterium in rectum and its relationship with type-B streptococcal rectal colonization and premature infections and its complications, it is recommended to conduct more studies in pregnant women.
Ahmad Talebian, Hamid Reza Sherkatolabbasieh, Mohsen Arbabi, Sayed Gholam Abbas Moosavi,
Volume 8, Issue 4 (1-2005)
Abstract
Background: According to high incidence of fever and its side effects in children and also because of different effects of drug regimens on reducing fever, we decided to investigate three kinds of adminstration of acetaminophen (aral with regular dosages, Rectal with regular and high dosages) on children whom were admitted in shaheed Beheshti Hospital in kashan in 2004. Materials and Methods: This randomized clinical trial was performed among 120 patients aged 6 months to 6 years with fever (axillary temperature ³ 38 ° c). Patients divided in 3 groups. Group A (40 patients) received oral acetaminophen with dose of 10-15 mg/kg acetaminophen. Group B and C received acetaminophen rectally with dose of 10-15 mg/kg and 30-60 mg/kg,respectively. Recovery was defined as reducing axillary temperature at least 1 ° c.Data analysis was performed using Chi Square test. Results: The frequency of recovery in groups A, B and C after 1 hour of adminstration of acetaminophen was 34 (85%), 32 (80%) and 36 (90%), respectively (p<0.05). It was 37 (92.5%), 38 (95%) and 39 (97/5%) after 3 hours (p<0.001) and 10 (25%), 14 (35%) and 22 (55%) after 6 hours (NS) of administration of acetaminophen in groups A, B and C, respectively Conclusion: High dose rectal acetaminophen is much more effective in reducing fever of children in comparison of regular dose of acetaminophen, orally or rectally.
Rahim Golmohamadi, Mahdi Nikbakht, Masour Salehi, Modjgan Mokhtari,
Volume 10, Issue 2 (7-2006)
Abstract
Background: Colorectal cancer (CRC) is a common cancer througout the world. Incident rate of CRC is different depending on geographical area. The cause of CRC is multifactrial, including diet, environment and genetic. P53 gene is the most important tumor suppressor gene involved in CRC. This study was designed to detect the P53 exon 6 mutation in the colorectal cancers at Isfahan University of Medical Sciences in 2004-2005. Materials and Methods: This study was performed on 40 colorectal patients cancer reffering to Isfahan Hospitals from 2004 to 2005. After pathological diagnosis, DNA was extracted by phenol chloroform isoamil alcohol in genetic. Exon 6 of the P53 gene was mutiplied using specific primers in a PCR assay and then the mutations were detected by gel electrophoresis and SSCP analysis. Results: From 40 specimens, 9 (22.5%) were in the rectum and 31 (77.5%) were in the colon. 7 cases (17.5%) had P53 mutation and 33 cases (82.5%) had no mutation in exon 6. Conclusions: According to this study exon 6 P53 gene mutation could be considered as a current exon in colorectal cancers in Isfahan.
Mohammad Nabiuni, Homa Kouchesfahani, Sakineh Azari , Bahram Delfan, Sedigheh Gholami, Azam Yarahmadi ,
Volume 16, Issue 6 (1-2013)
Abstract
Background: Colorectal carcinoma is the third most common type of cancer and the second most common cause of cancer-related mortality in the world. The aquaporins (AQPs) are water channel proteins that play a major role in water movements through epithelial and endothelial tissues. Expression of AQP5 was induced in the early stages of colon cancer. An induction of AQP5 expression in colon cancer suggests a probable driving force roles for AQP5 in colon carcinogenesis. Curcumin, as a chemopreventive phytochemical is important to block, retard or reverse the process of carcinogenesis. Several studies have suggested that curcumin may prevent or delay the occurrence of colorectal cancer. This study aimed to examine the effect of curcumin on the inhibition of AQP5. Materials and Methods: In this experimental study, the HT-29 cell line was cultured in DMEM medium containing 10 % FBS and 100 U/ml penicillin and 100 mg/ml streptomycin. The effect of curcumin concentrations on the growth of cells was determined using the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Immunocytochemistry was performed to examine the effect of curcumin on the expression of AQP5. Results: Immunocytochemistry showed the decreased amount of AQP5 protein in the curcumin-treated cells. Conclusion: Curcumin inhibits the expression of AQP5 in human colorectal cancer cell line, HT-29. The inhibition of AQ5 expression may provide a novel therapeutic target for the treatment and prevention of colorectal cancer.
Fakhrodin Naghibalhossaini, Zahra Khoshdel, Mostafa Moradi , Shahla Shojaei, Abbas Ghaderi,
Volume 16, Issue 7 (3-2013)
Abstract
Background: In recent years, measurement of the trace elements in human serum has been used for cancer diagnosis. The purpose of this study was to compare the serum concentrations of Cu, Zn and Cu /Zn ratio of colorectal cancer (CRC) patients with those of healthy subjects. Materials and Methods: This study was conducted on 130 patients with CRC (57 women and 73 men) and 128 health subjects (65 women and 63 men). Flame atomic absorption spectroscopy method was used to analyze the serum trace element concentrations. Results: The results showed that the serum Cu levels were significantly lower (P<0.01) in patients with CRC than the healthy controls, but no significant difference was observed between serum concentration of zinc in patients and healthy subjects. The concentrations of Zn in patients and healthy subjects were (68.38±44.76 vs. 142±37.61 ug/dl) and (84±53.51 vs. 140.37±63.43), respectively, in female and male subjects. Serum concentrations of Cu were (139±139.92 vs. 160.44±46.38 ug/dl) and (123.50±77.76 vs. 160.93±44.15 ug/dl), respectively, in female and male subjects. However, the Cu/Zn ratio increased significantly in both male (2.64±2.98 vs. 1.42± 0.88) and female (3.02±3.90 vs. 1.22±0.60) subjects. Conclusion: The serum copper levels and the Cu/Zn ratio may have an instrumental role in CRC development or can be used as a prognosis marker.
Ali Taheri, Mgmostafa Ghaffari, Zeinab Bavi, Fariborz Sohili,
Volume 22, Issue 3 (6-2018)
Abstract
Background: Seaweed is one of the natural resources with a wide range of secondary metabolites, which has cytotoxic properties. The present study aimed to investigate the cytotoxic effect of seaweed Sargassum glaucescens collected from Chabahar seashores against colorectal and breast cancer cells.
Materials and Methods: The cytotoxicity of methanol, chloroform, ethyl-acetate and hexane extracts of the seaweed at different concentrations (125, 250, 500 and 1000 μg/ml) against cancer cells of MCF-7 and HT-29 was evaluated by MTT and trypan blue methods. The DNA fragmentation of cells was also investigated by the electrophoresis method.
Results: The percentage of live cells was decreased by increasing the concentration of the extracts. The concentration of 1000 μg/ml of methanol extract showed the highest effect compared to the control and also lower concentrations of the extract (P<0.05). The LC50 of the methanol extracts of colorectal and breast cancer cells were 630.8±16.37 and 774.01±28.07 µg/ml, respectively. Also, the algal methanol extract was able to fragment the DNA of cancer cells and to induce apoptosis compared with the control samples.
Conclusion: The cytotoxic effect of the seaweed extract can be the beginning of further studies to achieve cancer treatment. Also, after pre-clinical and clinical studies, these extracts can be used in the food and pharmaceutical industries.
Hadi Habibollahi, Najmeh Ranji, Zeinab Khazaei-Koohpar, Hanieh Sadat Kamalifar,
Volume 23, Issue 6 (12-2019)
Abstract
Background: Familial adenomatous polyposis (FAP) is a hereditary precancerous syndrome and is characterized by the manifestation of adenomatous polyps in the colon and rectum at an early age. Germline mutations of APC gene cause FAP. This study aimed to investigate about the part of 3'-end of exon 15 of APC gene in FAP patients in Guilan, Ilam and Lorestan province in 2018.
Materials and Methods: In this descriptive cross-sectional study, 18 FAP patients were recognized and Blood sampling was done. After DNA extraction, a part 3'-end of exon 15 of APC gene was amplified by PCR method and underwent direct sequencing.
Results: In this study one nonsense mutation (c.4606G>T, p.E1536X) in a classic FAP patient and one missense mutation (c.5465T>A, p.V1822D) in an AFAP as homozygote and four classic FAP patients as heterozygote was observed. Also, four silent mutations p.T1493T, p.G1678G, p.S1756S and p.P1960P were identified in these FAP patients.
Conclusion: It seems that mutation E1536X is the main reason of disease in a patient with severe polyposis. Also, mutation V1822D as homozygous can cause AFAP; but for classic FAP development a more destructive mutation is needed along with this mutation.
Alimohammad Bananzadeh, Mohammadreza Joghataei, Mohammad Rezazadehkermani, Faranak Bahrami, Seyed Mohammad Kazem Tadayon, Leila Ghahramani,
Volume 24, Issue 6 (12-2020)
Abstract
Background: One of the rare but complicating consequences of laparoscopic surgery is port site hernia, while leaving the fascia open in the site of less than 10mm port is generally accepted. It still remains a debate whether to close the site of 10 mm ports or leave the fascia open. This study aimed to evaluate the incidence of port site hernia following laparoscopic colorectal resections.
Materials and Methods: between 2014 to 2016, files of all of the patients who had undergone laparoscopic colorectal resection in Shiraz Faghihi Hospital were reviewed retrospectively. Demographic data (age, sex and BMI) were extracted from the database. Then patient’s file was also reviewed for the kind of resection, port configuration, recovery issues, port site fascia closure and incidence of port site hernia post operatively. Gathered data were entered to SPSS database using descriptive (frequency, dispersion and central tendency indices) and analytic (independent t test and chi square) statistics using version 20 of this software.
Results: Files of 325 patients (184 male) with mean age of 56.41±14.09 (interval: 25-94) years old and mean BMI of 24.05±5.56 Kg/m2 were reviewed. 205 patients (63.1%) were rectal cancer and the rest of them were colon cancer cases. During study no case of port site hernia was detected and the prevalence of port site hernia was zero during one year follow up.
Conclusion: this study showed that not closing the fascia at the site of 10 mm ports was a safe practice and was not related to postoperative port site hernia.
Shahin Aghamiri, Shohreh Farhadi, Hossein Ghanbarian, Kazem Sharifi,
Volume 28, Issue 1 (3-2024)
Abstract
Background and Aim: 7SK is a long non-coding RNA that interacts with various proteins to regulate gene transcription. This study aimed to assess the impact of exosomal delivery of 7SK on viability, expression of apoptosis-related genes, and migration in the human colorectal cancer cell line HT-29.
Methods: In this experimental study, HT-29 cells were treated with exosomes derived from human umbilical cord mesenchymal stem cells loaded with 7SK (Exo-7SK). Control groups included cells treated with unloaded exosomes and untreated cells. The levels of 7SK in the cells, and expression of apoptosis-related genes were measured by real-time PCR, cell viability assessed by the MTT assay, and cell migration evaluated using the transwell assay.
Results: Treatment of HT-29 cells with Exo-7SK resulted in increased levels of 7SK, reduced viability, downregulation of the anti-apoptotic gene BCL-2, upregulation of the apoptosis-inducing gene BAX, and decreased migration.
Conclusion: Exosomal delivery of 7SK can effectively decrease the viability and migration of colorectal cancer cells. Further research is warranted to explore the therapeutic potential of this approach in colorectal cancer.
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