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Showing 6 results for Zargar
Madam Mansoureh Irani Khah, Madam Razieh Nazari, Mr Mahdi Fasihi-Ramandi, Mr Ramezan Ali Taheri, Mr Mohsen Zargar,
Volume 0, Issue 0 (1-2025)
Abstract
Background: In Brucella species, various protein and polysaccharide antigens have been identified in the outer membrane, cytoplasm, and periplasmic space. Several of these antigens, including Omp31, TF, and Bp26, have the ability to stimulate the host's immune system. The objective of the present study was to prepare a polyethyleneimine nanostructure containing a triple chimeric protein of Brucella as a candidate for a vaccine.
Materials and Methods: After designing the immunogenic construct using bioinformatics databases, cloning and gene expression were performed in Escherichia coli BL21 (DE3) to evaluate the immunogenicity of the designed recombinant protein. The extracted protein was purified using Ni-NTA columns, and after incorporating the antigen into the polyethyleneimine nanostructure, various characteristics of the nanoparticles, including size, zeta potential, encapsulation efficiency, and antigen release, were assessed.
Results: Characterization results confirmed that the polyethyleneimine nanocarrier containing the triple immunogen was successfully constructed. The average size of the nanoparticles containing the recombinant protein was approximately 100 nanometers (nm), and their zeta potential was around -45 millivolts. Over a 96-hour study period, approximately 84% of the encapsulated protein was released.
Conclusion: The results of our study indicated that the designed recombinant protein, in conjunction with the nanoparticle, possesses significant potential as a subunit vaccine candidate against Brucella species.
Mahriar Zargari, Samineh Motallebi Reikandeh,
Volume 16, Issue 7 (Supplement 2013)
Abstract
Background: The aim of this study was to evaluate the activity of delta-aminolevulinate dehydratase (δ-ALA-D) in red blood cells of haemodialysis patients and establish its association with serum levels of iron and zinc and lipid peroxidation. Materials and Methods: Blood samples of thirty patients (before hameodialysis) were collected to estimate the parameters of the δ-ALA-D activity in red blood cell (RBC), the serum levels of iron, zinc and thiobarbituric acid reactive substances (TBARS) and to compare with the thirty blood samples in the control group. Results: The results showed that the haemodialysis patients had a significant reduction inRBC count, haemoglobin concentration and haematocrit compared to the control group (P<0.05). The activity of δ-ALA-D in the red blood cells was significantly increased (P<0.001). The activity of δ-ALA-D in the red blood cells had a significant negative correlation with haematocrit (r=-0.61) and haemoglobin (r=-0.70) in the patients (P<0.05). In addition, there was a significant decrease in the serum iron and zinc levels, but an increase in the TBARS levels (P<0.05). The activity of δ-ALA-D in blood was negatively correlated with the levels of iron (r=-0.68) and zinc (r=-0.57). Conclusion: It can be concluded that the increased activity of δ-ALA-D in blood might have occurred in response to the anaemia in remission as heme synthesis is enhanced.
Kolsoom Dinarvand, Mahriar Zargari ,
Volume 16, Issue 7 (Supplement 2013)
Abstract
Background: The metals, including Chromium (Cr), Nickel (Ni), Cadmium (Cd), Arsenic (As), Zinc (Zn) and Selenium (Se) are considered trace elements and due to their limited quantities are essential to the human body. These elements are required as the essential components of biological enzyme systems or structural proteins. Deficiency in any of these elements leads to undesirable pathological conditions. Considering that cancer is a leading cause of mortality worldwide, our purpose of this study was to review the effect the trace elements on carcinogenicity or health maintenance. Materials and Methods: Scopus, Pubmed, Google scholar and ScienceDirect databases were searched. Different criteria were applied to select the related articles. Results: Results showed that the carcinogenicity of compounds of Chromium, Nickel, Cadmium and Arsenic are well-known. DNA repair systems are very sensitive targets for these elements that can cause a diminished removal of endogenous DNA lesions and increase the risk of tumor formation. Epidemiologic studies showed that Chromium and Nickel are associated with nasal and lung cancer in humans. Also, Arsenic and Cadmium exposures are associated with the increased lung cancer risk. On the other hand, many studies revealed that there was an inverse relationship between selenium exposure and risk of prostate cancer as well as between Zinc and breast cancer. Conclusion: Although the environmental exposure is a precondition for aggregation of toxic elements, but the individual characteristics and genetic factors are also involved. The effects of trace elements are dependent to many factors.
Ali Shivaee, Maryam Meskini, Shahla Shahbazi, ِdonya Hassani, Faramarz Masjedian Jazi, Mohsen Zargar,
Volume 23, Issue 2 (Bimonthly 2019)
Abstract
Background: Klebsiella pneumoniae is an opportunistic microorganism causing nosocomial infection all over the world .This study aimed to investigate the prevalence of biofilm formation in K. pneumoniae isolated from patients and its correlation with the virulence factors.
Materials and Methods: Biochemical tests were used for the identification of K. pneumonia isolated from patients referred to Motahari and Milad hospitals in Tehran, Iran, from October 2015 to June 2016. Kirby-bauer test was performed and biofilm formation was assessed phenotypically. Finally, virulence genes were detected by the PCR method.
Results: The highest resistance rate was against ceftazidime and cefotaxime (67%) and the least resistance rate was against imipenem and meropenem (39%). In addition, 81% of the isolates were biofilm producers according to the results of biofilm formation assay. Also, the results of PCR showed that all 57 biofilm producer isolates harbored fimA, mrkA, ecpA, and fimD virulence genes and 92% of these isolates harbored fimH virulence gene. Among non-biofilm producer isolates, 36% had fimA gene, 29% had ecpA gene, and none of these isolates carried mrkA and fimH genes.
Conclusion: It seems that antibacterial resistance has a significant association with biofilm formation in K. pneumoniae isolates. Therefore, understanding resistance pattern and mechanisms leading to biofilm formation can facilitate efficient treatment of infections caused by this bacterium.
Dr. Hamed Emami, Dr. Mehrdad Jafari, Dr. Behrooz Amirzargar, Dr. Amin Amali,
Volume 25, Issue 3 (Bimonthly 2021)
Abstract
Background: Otosclerosis is a bone deforming disease of the otic capsule. The objective of our study was to evaluate the effect of stapedotomy on tinnitus in patients with otosclerosis.
Materials and Methods: Nineteen patients with otosclerosis who suffered from tinnitus and conductive hearing loss and scheduled to undergo stapedotomy were enrolled in the study. The incidence and severity of tinnitus were recorded using a visual analog scale (VAS) questionnaire before and after surgery. Tinnitus scores were compared according to the VAS.
Results: Mean age of patients was 37.3±10.8. Tinnitus disappeared in 47.4% of the cases, improved in 36.8%, and was unchanged in 15.8% of the patients. Mean tinnitus scores before and after the surgery were 6.4±1.9 and 3.1±2.4 respectively. Comparison of the tinnitus score revealed statistically significant improvement in the tinnitus perception of the patients who underwent stapedotomy (P<0.001).
Conclusions: In otosclerotic patients who will undergo stapedotomy for hearing improvement, stapes surgery may improve tinnitus, also.
Fahimeh Sharif, Razieh Nazari, Mahdi Fasihi-Ramandi, Ramezan Ali Taheri, Mohsen Zargar,
Volume 27, Issue 1 (Bimonthly 2023)
Abstract
Background: Brucellosis is one of the most common diseases between humans and animals. Therefore, the need for prevention and production of an efficient vaccine is necessary. The aim of the present study is to prepare a niosomal nanostructure containing trivalent immunogen (TF, Bp26 and Omp31) of Brucella as a nanovaccine candidate.
Materials and Methods: In this study, after designing the immunogen structure by bioinformatics databases and software, in order to evaluate the immunogenicity of the designed recombinant protein, clone and gene expression were carried out in E.coli BL21. The protein extracted from the culture of said cells was purified by Ni-NTA column. Thin layer coating method was used to prepare niosomes carrying trivalent immunogen and were evaluated using DLS and Zetasizer characterization tests. Then, the amount of trivalent immunogen loading and release was calculated.
Results: The results of the characterization confirmed the successful fabrication of niosomal nanosystem containing trivalent immunogen. The results showed that the produced niosomes are in a suitable range of size (100 nm). Trivalent immunogen with high efficiency (81.96%) is encapsulated in the system. The release of trivalent immunogen from Niosomal nanosystem was reported to be 97% after 96 hours. In addition, the trivalent immunogen release pattern of the coated system was more controlled and slower. This confirms the positive effect of the niosome nanosystem.
Conclusion: The use of nisome as a nanovaccine agent has an effective role in controlling antigen release and can be considered as a vaccine candidate and increase the protective immune response against Brucella.
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