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Showing 10 results for Rezazadeh
Hossein Nikzad, Mojtaba Rezazadeh, Ahmad Hosseini, Shams Shariat Torbeghan, Sayed Gholam Abbas Mousavi,
Volume 2, Issue 4 (Quaterly 1999)
Abstract
History and Objectives: vasectomy is one of the prevalent method approved by the Ministry of Health and Medical Education for prevention of male reproductive competency. Due to discrepancies in the reported cases of the effect of vasectomy on the testicular tissue of human and animal models, in order to determine the long term affected of vasectomy on the general appearance and histology of testicular tissue, the present study was carried out in Tarbiat Modares university in 1995. Materials and Methods: An experimental study on 14 Wistar rats was performed. They were randomly divided equally into 2 vasectomy and shame operated groups: A bilateral surgery was performed on all rats. Changes in the testicular tissue were assessed by taking histologic samples (With hematoxillin-eosin, PAS and trichrome mission staining) 6 months after the surgery. Weight and volume of testis, seminiferous tubule diameter, epithelium thickness of seminiferous tubule, testicular volume percentage and number of epithelial germinal sertoli cell of seminiferous tubule were recorded. Data gathered from 2 groups was compared and statistical analysis was done. Results: vasectomy induces changes in increased folding of membrane of seminiferous tubule, vacuolization of epithelium seminiferous tubule and desquamation of immature germ cells, in vasectomized group. Comparison of the changes of variables under investigation in 2 groups indicates that there is a significant difference between the 2 groups. Testicular weight (1.2±0.2 in vasectomized group compared to 1.5±0.2 g in control group), testicular volume (1.1±0.2 cm³ compared to 1.4±0.2 cm³) (P<0.0005), seminiferous tubular thickness 225±53 micron compared to 78±15.1 micron) (P<0.0001) showed significant reduction. In addition, testicular volume percentage and number of germ cells in 2 groups showed significant difference (P<0.0001). However, number of sertoli cells did not changed significantly. Conclusion: Vasectomy induces significant changes in the rat testicular tissue which further study is required to elucidate the underlying mechanism and extrapolation of the results to humans.
Tahere Mazouchi, Mojtaba Rezazadeh, Ahmad Hosseini,
Volume 4, Issue 1 (Quaterly 2000)
Abstract
History and Objectives: Due to importance of developmental studies and specially fingers formation and since recent reports indicated a role for apoptosis in the development of free fingers, the present study was carried out to on chick embryo with free fingers as the experimental group and duck embryo with interdigital membrane as control group. Materials and Methods: An analytical cohort study on white Leghorn eggs and Royal Pekin duck eggs were carried out. Chick embryos were at sixth and 9th days of incubation (Stage 29-35) and duck embryos at eight and half and 11th day of incubation at 12 hour intervals were taken for study. In the present study, neutral red as a vital color by Hinchiffe & Ede method was selected. The staining is specific for apoptotic and macrophage cells. Embryo legs were monitored by stereomicroscope and pattern of apoptosis were followed. Results: Interdigital cellular apoptosis was observed in chick and duck embryos at 6.5 days and reached a pick level at 7.5 day and ended at 8.5 day when interdigital tissue had complete regression. Interdigital tissue apoptosis for the four fingers of foot was obvious in chick as well duck embryos however the extent of apoptosis was less in duck embryos. Interdigital cellular apoptosis started at the 9th day and continued until the 9.5 day and terminated at 11th day. Interdigital membrane between the first and second finger show similar necrotic region whereas the fingers formation is quite different. Conclusion: Apoptotic pattern of interdigital membrane of chick and duck is accompanied by removal of mesenchyma and sculpturing and separation of fingers. Comparison of apoptotic intensity reveals other possible factors for the separation of fingers. It is suggested that extended study should be carried out to unravel the mechanism of interdigital regression at cellular as well as genetic level.
Tahereh Mazoochi, Mogdeh Salehnia, Mojtaba Rezazadeh Valojerdi, Sayed Javad Mowla, Ibrahim Hajizadeh,
Volume 11, Issue 1 (Quarterly 2007)
Abstract
Background: Vitrification is a simple and ultra rapid technique for the conservation of fertility. In this study, we compare the in vitro development of preantral follicles obtained from fresh specimens with vitrified- warmed mouse ovaries. Materials and Methods: This experimental study was carried out on 20, 14-day-old female mice (NMRI). Ovaries were vitrified with a solution containing ethylene glycol, ficoll 70 and sucrose in PB1 (EGFS40) for 5 min, and transferred directly into liquid nitrogen and stored for one week. Fast warming was done by descending sucrose at room temperature. Preantral follicles with 100-130 µm in diameter were mechanically isolated from fresh and vitrified-warmed ovaries and cultured in α-minimum essential medium (α-MEM) supplemented with 5% fetal bovine serum (FBS), 100 mIU/ml rFSH, 1% ITS, and 20ng/ml mrEGF in vitro for 10 days. Diameter of follicle, and, survival rate and number of antral follicles in both groups were compared using t-test and chi-square test, respectively. Results: Isolated follicles from the vitrified and nonvitrified groups survived and grew in vitro culture. On the day 6 survival rates in the vitrified and fresh control groups were 72.1% and 78.6%, and, on the day 10, they were 66.9% and 72.6%, respectively. Follicle antrum formation was 37.5% in the vitrified group while it was 43.5% in the fresh group in the 10th day. On the day 2, the mean diameters of fresh and vitrified follicles were 158.11 ± 11.23 and 155.48 ± 8.35 and on the day 4, they were 201.56 ± 9.87 and 193.42 ± 8.46, respectively. There was no significant difference between the control and vitrified groups in these variables (p>0.05). Conclusion: Taken together, cryopreservation of the ovary by vitrification seems to be a promising method to preserve ovarian follicles.
Seyed Hani Davoodi, Majid Vahidian-Rezazadeh, Hamed Fanaei,
Volume 22, Issue 4 (Bimonthly 2018)
Abstract
Background: Diabetes mellitus is one of the most common metabolic diseases and nettle is a plant that has been introduced as an anti-diabetes medication. Nesfatin-1 is an adipokine secreted from an adipose tissue and involved in the mechanism for glucose metabolism. This study aimed at examining the effect of an 8-week endurance and resistance training program along with the consumption of the hydro-alcoholic extract of nettle on plasma levels of nesfatin-1 in type 1 diabetic rats.
Materials and Methods: In this study, Wistar rats were divided into four groups and diabetes was induced in them by streptozotocin. The nettle extract (1mg/kg) was intraperitoneally injected daily in the "nettle", "endurance+nettle" and "resistance+nettle" groups. The "endurance+nettle" group swam in water for eight weeks, five sessions per week, and each session from 60 to 120 minutes. The "resistance+nettle" group began the exercise with 50, 75, 90, and 100 percent weight on the designed ladder, and then they gradually increased their record. Blood samples were taken directly from the hearts of the animals at the end of the 8th week.
Results: Weight loss was observed in all groups after 8 weeks. The least amount of weights in the "control", "nettle" and "resistance+nettle" groups were significantly lower than those in the beginning of the study (P<0.001). The results showed a significant increase in nesfatin-1 in the "nettle" group compared to the "control" group (P<0.01). Also, this increase was observed in the "endurance+nettle" and "resistance+nettle" groups compared to the "control" group (P<0.001).
Conclusion: Consumption of the nettle extract with exercise can increase the plasma nesfatin-1, which can be effective in controlling diabetes and preventing weight loss due to this disease.
Sepideh Basirat-Dehkordi, Majid Vahidian-Rezazadeh, Ali Moghtaderi,
Volume 23, Issue 4 (Bimonthly 2019)
Abstract
Background: Multiple Sclerosis (MS) is associated with many pathological changes and liver damage is one of the most important consequences. This study aimed to investigate the effect of aerobic exercise and the royal jelly on AST, ALT, and ALP enzymes in the patients with multiple sclerosis.
Materials and Methods: In this semi-experimental study, 42 MS patients were divided into 4 groups: 1-"Exercise +Royal Jelly" (n=10), 2-"Exercise +Placebo" (n=10), 3-"Royal Jelly" (n=11), 4-"Placebo" (n=11). The Exercise started with an ergometer at 60% HRmax for 6 weeks, 3 sessions per week then increased steadily in the sixth week to 80% HRmax. The daily dose of the royal jelly was 1000 mg. Plasma concentration of the liver enzymes was measured before and after the study.
Results: The levels of AST, ALT, and ALP enzymes were significantly reduced compared to the pre-test in the groups of the "Exercise+Royal Jelly" and "Exercise+Placebo" (P<0.05). Also, the ALT enzyme showed a significant decrease in the "Royal Jelly" group. The results of the statistical analysis indicated a significant reduction of the enzymes in the "Exercise+Royal Jelly" and the "Exercise + Placebo" groups compared to the "control" and the "Royal Jelly" groups at the end of the sixth week (P<0.05).
Conclusion: It seems that the aerobic exercise and the royal jelly consumption together had the most decreasing effect on serum levels of the liver enzymes in the multiple sclerosis patients, and It has a positive effect on the hepatic enzymes and especially the levels of ALT enzymes in patients.
Alieh Shahraki, Majid Vahidian-Rezazadeh, Morteza Nikoofar,
Volume 24, Issue 3 (Bimonthly 2020)
Abstract
Background: This study aimed to investigate the effect of endurance and combination training on serum levels of Fetuin-A and metabolic syndrome of overweight and obese women.
Materials and Methods: This was a semi-experimental study and 45 overweight and obese women were randomly divided into three groups: “control”, “aerobic exercise” and “combined exercise” groups. The aerobic exercise and combined exercise groups were active for eight weeks. After blood sampling, Fetuin-A, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglyceride, total cholesterol and glucose were measured.
Results: The results showed that after eight weeks in the aerobic and combined groups, Fetuin-A significantly increased (P=0.022, P=0.011). Also, LDL was significantly decreased in aerobic and combined groups (P=0.006, P=0.024). The results showed that the combined group had a significant decrease in TC, TG, FAT, BMI, and WHR compared to baseline and a significant increase in HDL (P<0.05). Also, Fetuin-A decreased in aerobic and combined groups compared to the control group. The combined group showed a significant decrease in TC compared to the control and aerobic groups (P<0.05). Besides, the combined group had lower TG, FAT, BMI, and WHR indices compared to the control group (P<0.05).
Conclusion: The results of this study showed that combined exercises compared aerobic training had significant decreasing effects on Fetuin-A, as well as metabolic syndrome indices in obese and overweight individuals and contributes to the improvement of the dangers of obesity and cardiovascular risk factors.
Alimohammad Bananzadeh, Mohammadreza Joghataei, Mohammad Rezazadehkermani, Faranak Bahrami, Seyed Mohammad Kazem Tadayon, Leila Ghahramani,
Volume 24, Issue 6 (Bimonthly 2020)
Abstract
Background: One of the rare but complicating consequences of laparoscopic surgery is port site hernia, while leaving the fascia open in the site of less than 10mm port is generally accepted. It still remains a debate whether to close the site of 10 mm ports or leave the fascia open. This study aimed to evaluate the incidence of port site hernia following laparoscopic colorectal resections.
Materials and Methods: between 2014 to 2016, files of all of the patients who had undergone laparoscopic colorectal resection in Shiraz Faghihi Hospital were reviewed retrospectively. Demographic data (age, sex and BMI) were extracted from the database. Then patient’s file was also reviewed for the kind of resection, port configuration, recovery issues, port site fascia closure and incidence of port site hernia post operatively. Gathered data were entered to SPSS database using descriptive (frequency, dispersion and central tendency indices) and analytic (independent t test and chi square) statistics using version 20 of this software.
Results: Files of 325 patients (184 male) with mean age of 56.41±14.09 (interval: 25-94) years old and mean BMI of 24.05±5.56 Kg/m2 were reviewed. 205 patients (63.1%) were rectal cancer and the rest of them were colon cancer cases. During study no case of port site hernia was detected and the prevalence of port site hernia was zero during one year follow up.
Conclusion: this study showed that not closing the fascia at the site of 10 mm ports was a safe practice and was not related to postoperative port site hernia.
Maryam Akhavan-Taheri, Mojtaba Rezazadeh Valojerdi, Bita Ebrahimi,
Volume 25, Issue 1 (Bimonthly 2021)
Abstract
Background: One of the problems after cryopreservation and ovarian tissue transplantation is ischemia followed by follicles mortality. In the present study, hyaluronic acid hydrogel was used to improve angiogenesis in vitrified ovarian tissue transplantation in rat.
Materials and Methods: In this experimental study, 22 adult female rats (~8 weeks old) with normal estrous cycles were ovariectomized, then their right ovaries were vitrified and warmed into two groups without hyaluronic acid hydrogel (VT) and encapsulated with hyaluronic acid hydrogel (VT+HA) was autotransplanted into the dorsal muscle. Daily vaginal monitoring was performed until re-initiation of first full oestrus cycles. The ovaries were removed at the end of the first estrus cycle and angiogenesis genes VEGF, CD31 and CD34 evaluated by real time PCR. Data were analyzed by Mann-Whitney test and the significance level was considered P<0.05.
Results: All transplants were completely successful (100% successful transplant). There was no statistically significant difference in CD34 and CD31 genes between two groups. But VEGF gene expression was significantly lower in VT+HA group (0.25±0.06) than VT group (1.00±0.05) (P<0.05). Thus, the VT group appeared more successful than the VT+HA group in expressing angiogenic genes at the end of the first estrus cycle.
Conclusion: Hyaluronic acid hydrogel does not play any effective role in the growth and improvement of angiogenesis after transplantation in the short term. But due to the angiogenic property of hyaluronic acid, increasing the expression of angiogenic genes in the VT + HA group may need more time.
Tayyebeh Zamani-Badi, Hossein Nikzad, Atieh Rafatmanesh, Zeinab Rezazadeh Lavaf, Mohammad Karimian,
Volume 26, Issue 1 (Bimonthly 2022)
Abstract
Background: Infertility is one of the major health problems in the world and several factors play a role in the occurrence of this complication. One of the causes of idiopathic male infertility is a defect in the process of spermatogenesis due to genetic changes in cytokines involved in this process. Tumor Necrosis Factor-alpha (TNFα) as a multifunctional cytokine controls spermatogenesis-related cellular activity. In this study, the association of -308G/A polymorphism in the TNFα gene with male infertility was investigated.
Materials and Methods: In a case-control study, blood samples were collected from 82 infertile men and 107 fertile men. After DNA extraction, the genotype of the samples at the -308G/A region was determined using the PCR-RFLP technique.
Results: Data analysis showed a significant association between GA genotype and reduced risk of male infertility. Also, in the subgroup study, a significant association was observed between this genotype and the reduction of oligozoospermia and asthenozoospermia risk. Similar results were found for the association of carriers of allele A (GA + AA) and idiopathic male infertility. In addition, the allelic analysis showed a significant association between allele and a reduced risk of idiopathic male infertility. Subgroup analysis showed a significant association between this allele and reduced risk of asthenozoospermia, also.
Conclusion: Based on findings of this research, the TNFα -308G/A polymorphism can be considered as a protective factor and a potential biomarker for idiopathic male infertility.
Hossein Rezazadeh Mehrizi, Nepton Soltani, Nasrin Mehranfard, Maedeh Ghasemi,
Volume 27, Issue 5 (Bimonthly 2023)
Abstract
Background and Aim: Evidence shows that glutamate receptors play a role in the occurrence of gastrointestinal damage caused by diabetes. Therefore, in this study, the protein expression pattern of glutamate receptor N-methyl-D-aspartate (NMDA) and pannexin-1 (Panx-1) channel, which has an important functional relationship with glutamate receptors, in the stomach of type 2 diabetic rats were investigated. We also assessed the effect of long-term treatment with insulin on the possible changes of the mentioned proteins.
Methods: In this experimental study, 18 male Wistar rats weighing approximately 80-90 grams were randomly assigned into 3 groups (6 rats each): 1) control with standard diet, 2) type 2 diabetes: high-fat diet for 3 months + single dose of streptozotocin (35 mg/kg), and 3) type 2 diabetes + insulin treatment. The protein content of NMDA receptor as well as Panx-1 was detected using western blot method and compared among groups.
Results: In the stomach of type 2 diabetic rats, the expression of Panx-1 protein was significantly decreased (P<0.001), while the expression of NMDA receptor (P<0.001) showed a significant increase. Insulin treatment improved the expression level of Panx-1 protein in diabetic rats, in addition, insulin treatment decreased the expression level of NMDA receptor in diabetic rats (P<0.05).
Conclusion: The present findings indicate important changes in protein levels of NMDA receptor and Panx-1 channel and their possible role in the occurrence of sensory-motor and secretory damage related to diabetes. The correction of the above changes may play a role in improving the functional damage caused by type 2 diabetes.
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