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Showing 24 results for Kazemi
Saeid Kazemi Ashtiani, Mansour Jamali Zavarehi, Naser Salsabili, Siamak Bashardoust,
Volume 4, Issue 1 (Quaterly 2000)
Abstract
History and Objectives: Increase in attention is paid for the use of various techniques such as ultrasound to improve connective tissue healing. The present research will study the effect of ultrasound therapy on bone regeneration in rabbits from histological point of view. Materials and Methods: In an experimental study, 28 white male Dutch rabbits with 3 month of age and 1200gr weight underwent medical open partial osteomy of tibia bone under general anesthesia and sterile conditions. Rabbits were divided randomly into experimental and control groups. In each seven subgroup (5th, 7th, 9th, 13th, 15th, 20th and 27th days) were formed. The experimental group was treated with 1 w/cm² ultrasound pulse 2:8 with 1.5MHz frequency for 5 min for 7 days (As mentioned above) against control group. Measurements wee done in above mentioned time intervals after surgery. After the time intervals, animals were killed and tibia was examined for fracture macroscopically as well as microscopically. Results: Histological findings (Light microscopy measurements indicated that healing process in experimental group was faster compared to control group in inflammation and proliferation phase (15th days after surgery). Healing process in experimental group was slower than control group in callus formation and remodeling phase (From 15th to 27th days after surgery). Conclusion: Ultrasound therapy with 1.5MHz frequency accelerates bone healing in initial stage (Inflammation and proliferation) in rabbits.
Homayou Naderian, Bahram Kazemi, Af de Vries,
Volume 11, Issue 1 (Quarterly 2007)
Abstract
Background: Gene therapy is an important technique in clinical research which offers new visions for the treatment of genetic deficiencies. Gaucher disease caused by genetic deficiency of glucocerebrosidase (Gba) enzyme has attracted special consideration in gene therapy. The aim of this project is cloning and transfering of glucocerebrosidase enzyme gene to HEK cell line by enhanced lentiviral vector. Materials and Methods: The cDNA of glucocerebrosidase enzyme gene was synthesized, amplified with specific primers by PCR methods, cloned in non-expressing vector and sequenced. The recombinant gene was subcloned in enhanced lentiviral vector by GFP reporter gene. After culturing the HEK cell line, the recombinant lentiviral vector was transferred to them and the transfer of Gba gene was examined by GFP reporter gene. Results: The amplification and cloning of glucocerebrosidase enzyme gene was confirmed by restrictive enzymes. The sequence of Gba gene was compared correctly by its reported sequence. Subcloning of Gba gene in lentiviral vector was confirmed by different restrictive enzymes. The transfer of Gba recombinant gene was confirmed by reporter gene with flurecent proteins. Conclusion: This project is a part of gene therapy protocol performed by the transferring of mouse glucocerebrosidase enzyme gene to HEK cells by lentiviral vector.
Mahdi Ghadri Golestani, Seyyed Hamidreza Aghayan, Babak Arjmand, Anusheh Kazemian, Seyyed Amirhosein Tavakkoli, Seyyeyed Kazem Hoseini, Farrokh Tiregari, Seyyed Mohammadjavad Mortazavi,
Volume 11, Issue 4 (Quarterly 2008)
Abstract
Abstract: Background: Ethylene oxide is widely used for secondary sterilization of bone allograft to reduce the risk of infection and associated complications. In this study the effects of ethylene oxide gas sterilization on the osteoinductivity of demineralized bone powder is investigated.
Materials and Methods: Two separate implants consisting 30 mg aseptically prepared, and 30 mg ethylene oxide-sterilized demineralized bone powder was placed into two different muscle pouches created in the paravertebral muscles of eighteen rats. After 4 weeks, they were removed with 0.5 cm normal surrounding tissue. Histological examination was done to determine the presence or absence of osteoinduction.
Results: All except one of eighteen aseptically prepared demineralized bone powder sites histologically contained new bone elements (94.4 %) and fourteen (77.7%) out of eighteen ethylene oxide sterilized demineralized bone powder sites showed evidence of new bone elements. There is no significant difference in osteoblast formation in the two groups (p>0.05).
Conclusion: It is concluded that ethylene oxide sterilization in 42˚C did not significantly reduce the osteoinductivity of demineralized bone powder. So, it can be considered as a suitable but not perfect method for secondary sterilization of demineralized bone powder.
Mohammad Kazemi Arababadi , Nima Naghavi, Golam Hosein Hassanshahi, Sayyed Mohammad Ali Sajadi ,
Volume 13, Issue 3 (Quaterly 2009)
Abstract
Background: Although type-2 mellitus diabetes is the most common type of diabetes, it's main cause yet to be identified. Chemokines and their receptors are probable effective systems on diabetes. CCR5 is a chemokine receptor playing an important role in immune responses. Studies showed that the known 32 mutation in CCR5 gene leads to disorder in the expression and function of this receptor. Hence, this project aimed to analyze the known 32 mutation in CCR5 chemokine receptor. Materials and methods: Blood samples were collected from 200 type 2 diabetic patients and 300 healthy adult controls on EDTA pre-coated tubes. DNA was extracted using commercial kit. DNA samples were analyzed for 32 mutation by Gap-PCR in diabetic patients in compared to controls. The demographic information were collected through questionnaire. Results: Our results showed that none of the diabetic patients displayed CCR5 32 mutation. While 2 out of 300 healthy controls had heterozigotic form of this mutation. Statistical analysis didn’t show any significant difference between the two groups. Conclusion: Several different studies analyzed the relation of this mutation with different types of diseases including diabetes. All studies failed to find a relation between this mutation and type 2 diabetes. Since these studies were performed in different geographical points and races, we studied this mutation in Rafsanjanese population. Based on the results of our study it could be probably concluded that this mutation does not play a key role in the establishment of type 2 diabetes.
Safarali Talari, Bahram Kazemi , Hosain Hooshyar, Faegheh Kazemi, Mohsen Arbabi, Mohammad Reza Talari, Hamid Reza Nikyar, Ahmad Sobhani, Bita Zaree Kar , Akbar Tabibian ,
Volume 15, Issue 1 (Quarterly 2011)
Abstract
Background: Trichomoniasis is a worldwide protozoan parasitic disease. Considering the importance of the disease in public health and the controversial ideas about the prevalence of drug resistance, this study was designed to determine the prevalence of metronidazole resistance gene in trichomonas vaginalis (T. vaginalis) with PCR-RFLP method in Tehran and in Kashan. Materials and Methods: In this descriptive study 140 samples of T. vaginalis in patients with T. vaginalis infections were collected and assessed microscopically. Then they were isolated and examined by culturing in dorset's medium, DNA extraction and PCR amplification. The PCR products were analyzed using RFLP and suspected samples were sequenced. Results: All but 7 samples were T. vaginalis positive by PCR. Sixty-two samples (44.4%) were examined by microscopic, culture and PCR techniques 12 samples (8.5%) by microscope and PCR, 56 samples (40%) by culture and PCR and other 3 samples (2.1%) were positive only by PCR. Two samples (1.5%) were also examined for detection of mutation in 18S rRNA gene with RFLP in Tehran. Conclusion: This study shows that T. vaginalis infections in the female population living in Tehran are metronidazole-resistant. Since metronidazole is considered as the drug choice for T. vaginalis infections, more studies are recommended for identification of the drug resistance mechanisms and prevention of the disease.
Yadolah Ramezani , Sayyed Gholam Abbas Mousavi , Abbas Bahrami , Mohsen Fereydooni , Nasrin Parsa , Bahram Kazemi,
Volume 15, Issue 3 (Quarterly 2011)
Abstract
Background: Leishmaniasis caused by leishmania protozoa can be considered as a zoonotic disease. This disease caused major health problems in some parts of Iran. Aran and Bidgol city (Isfahan province, Iran) is considered as one of the endemic foci of cutaneous leishmaniasis (Salak). For success in controlling the disease and to provide the necessary training for high-risk groups, the epidemiological data of disease in the region is required. Materials and Methods: This cross-sectional study was carried out on 94 patients admitted to the health center of Aran and Bidgol from April to September 2009. The demographic and epidemiologic data were collected and analyzed. Results: Thirty percent (30.8%) of patients were in the age group of 1-9 years. The number of patients in urban areas was more than that of rural areas and in men more than women. Most of these cases were seen in Aran and Bidgol city (52.1%) and then in Abuzeydabad city (22.3%), respectively. Moreover, most cases of disease were found in July (37.2%) and 54.3% and 45.7% of cases were dry and wet cutaneous sores, respectively. Conclusion: It seems that the high incidence of disease in age group of 1-9 years is due to the susceptibility of this age group to leishmaniasis. Therefore, regulating the control training programs in this group is suggested.
Safarali Talari , Bahram Kazemi, Hosain Hooshyar , Roghayeh Alizadeh, Mohsen Arbabi , Sayyed Gholam Abbas Mousavi, Mohammad Reza Talari , Hamid Reza Nikyar , Sobhani ,
Volume 16, Issue 3 (Bimonthly 2012)
Abstract
Background: Cutaneous leishmaniasis is a common parasitic disease and one of the health problems world wide. The pentavalent antimonial drugs (e.g. pentostam and Glucantime) are the first line treatment for leishmaniasis, and resistance to these drugs is a serious problem. Using PCR method, this study was carried out to identify the mutation for sodium stibogluconate resistance gene in cutaneous leishmaniasis cases referred to different health centers during 2006-8. Materials and Methods: This descriptive study was conducted on 150 isolates of leishmania major and leishmania tropica to identify the mutation in drug resistance gene. Promastigote clones were cultured in enriched RPMI 1640 medium and then the genomic DNA was isolated and using a pair of primers, a 400 bp of the gene was amplified. Finally, the PCR products were screened by conformation sensitive gel electrophoresis (CSGE) method and then the mutation was confirmed using RFLP with Sdu1 enzyme. Results: Screening using CSGE and RFLP methods showed that 6.3% of the samples carried a mutation for drug resistance gene. Conclusion: Results showed a resistance for cutaneous leishmania against sodium stibogluconate. Further studies are required to determine the biochemical mechanism of this resistance.
Vahid Sobhani , Behzad Hajizadeh, Behzad Bazgir, Maryam Kazemipour , Alireza Shamsoddini, Abolfazl Shakibaey,
Volume 17, Issue 6 (Bimonthly 2014)
Abstract
Background: Omega (ω)-3 fatty acids have positive effects on different body systems such as cardiovascular, respiratory, nervous, and skeletal systems. The purpose of this study was to examine the effect of ω-3 supplementation on pulmonary function in cadets participated in army ranger training. Materials and Methods: In this study, 41 army cadets (mean age, 18.6±0.9 years) were randomly allocated to the supplement (S, n=21) and placebo (P, n=20) groups. The two groups participated in army classic military training 4-5 days/week for 8 consecutive weeks. The S and P groups were asked to consume 1000 mg/day ω-3 and placebo per day for 8 weeks, respectively. Pulmonary function was assessed at the first and end of the training. Results: Results indicated a significant difference in FEV1, FVC, MVV, FEF25-75%, FIV1 at the end of the training compared to those obtained at the beginning in both groups however, the changes were markedly greater in the S group (P≤0.05). Conclusion: It can be concluded that the supplementation of ω-3 (1000 mg/d) can induce positive alteration in different pulmonary function indices.
Gholam Hosein Kazemian, Alireza Manafi , Farideh Najafi, Mohammad Amin Najafi,
Volume 18, Issue 2 (Bimonthly 2014)
Abstract
Background: There are several methods to manage intertrochanteric fractures. The external fixation device could produce favorable clinical outcomes. Furthermore, it has a lower rate of morbidity. Therefore, this study aimed to compare two different methods for the reduction of the intertrochanteric fracture in elderly high-risk patients : external fixator vs. skeletal traction. Materials and Methods: In this randomized clinical trial, 60 elderly patients (mean age, 78 years) with a pertrochanteric fracture were randomly assigned to the two groups and treated with either skeletal traction (group A) or external fixation (group B). Results: Significant improvements were observed in all fractures acceptable reduction was achieved in 8 patients in group A and 26 patients in group B. The mean length of hospitalization was 14.3 days for group A and 2.2 for the group B. There was a significant difference between the two groups regarding the acceptable reduction of fracture and duration of hospitalization (P<0.05). At 12 months after surgery, the mean Harris hip scores were 57 and 66 in the group A and B, respectively. Conclusion: M anagement with external fixator is an effective method for intertrochanteric fractures in elderly high-risk patients. Its advantages include quick and simple application, minimal blood loss, less radiation exposure, early discharge from hospital, low costs and favorable functional outcomes.
Fatemeh Zolfaghari, Negin Pourzadi, Faezeh Sahbaei, Farzaneh Zolfaghari , Samaneh Sadat Kazemi, Ahmad Reza Davari ,
Volume 19, Issue 4 (Bimonthly 2015)
Abstract
Background: Type 2 diabetes is considered as one of the most common chronic diseases. Controlling glucose indices and blood pressure in diabetic patients is very important. This study aimed to examine the effect of blueberry solution on blood pressure and fasting blood sugar (FBS) in patients with non-insulin dependent diabetes mellitus.
Materials and Methods: In this before-and-after experimental study, 50 patients were selected and received 20 ml of blueberry solution before every meal. The FBS and blood pressure of the patients before and one, two, three and four weeks following the consumption were measured and the results were compared before and after the intervention.
Results: Sixty-six percent of the subjects were females and 60% of them were in the age group of 55-50 years old. The mean FBS level before the intervention was 190.50±53.48 mg/dl and mean values of the systolic and diastolic blood pressure before the intervention were 150.20±8.75 and 103.16±9.66 mmHg, respectively. After the intervention, there was a statistically significant reduction in mean FBS level (the first stage: 178.32±51.71, the second stage: 170.26±50.66, the third stage: 165.42±51.71, and the fourth stage: 156.08±51.49 mg/dl), systolic (the first stage: 147.24±8.67, the second stage: 144.18±8.52, the third stage: 142.14±8.47, and the fourth stage: 140.84±9.06 ml/Hg) and diastolic (the first stage: 98.52±7.63, the second stage: 94.44±6.72, the third stage: 92.12±6.05, and the fourth stage: 91.34±6.75 mm/Hg) blood pressure (P=0.001).
Conclusion: Medicinal plants such as blueberry can be used as a drug to reduce FBS and blood pressure in patients with diabetes.
Mohsen Kachooei, Alireza Moradi , Ameneh Sadat Kazemi , Zeynab Ghanbari,
Volume 20, Issue 4 (Bimonthly 2016)
Abstract
Background: Apart from important role of unhealthy eating patterns on eating-related problems (i.e. obesity and eating disorders), the underlying processes of such eating patterns are not fully understood. This study aimed to examine the role of emotion regulation and impulsivity in unhealthy eating patterns.
Materials and Methods: In this study which was carried out with a correlation method, the adult female (no=347) from Tehran completed the self-reported inventories. Dutch eating behavior questionnaire (DEBQ), difficulties in emotion regulation scale (DERS), and Barratt impulsivity scale (BIS-11) were used as measuring tools. Data was analyzed using Pearson correlational analysis and multiple regression analysis.
Results: The relationship between restrained eating and emotion regulation was significant (P<0.01). Emotion regulation predicted the engagement in restrained eating. There were a significant relationship between emotional eating and emotion regulation (P<0.01) and impulsivity (P<0.01). Emotion regulation and impulsivity predicted the engagement in emotional eating. In addition, the relationship between external eating and emotion regulation was significant (P<0.05). Emotion regulation can predict the engagement in external eating.
Conclusion: These findings highlight the importance of emotion regulation as a key risk factor in unhealthy eating patterns. Moreover, the restrictive eating patterns and binge eating patterns differ each other in terms of impulsivity. Treatment interventions must address the emotion regulation and include the strategies to cope with impulsivity.
Fatemeh Tajik-Ijdan, Ali Kazemi, Hossein Nowrozi,
Volume 21, Issue 3 (Bimonthly 2017)
Abstract
Background: Candidiasis is a prevalent disease which is caused by different species of Candida. Herbal drugs (e.g. ginseng) were traditionally administrated for the treatment of different diseases. This study was carried out to compare the effect of alcoholic extract of ginseng with Itraconazole against Candida albicans (C. albicans) and Candida krusei (C. krusei).
Material and Methods: This cross-sectional study was crried out on 22 and 8 species of C.albicans and 8 C.krusei, respectively which were isolated from vagina, urine and sputum of the patients. Using the CLSI M27 and disk diffusion methods the susceptibility test was done by Itraconazole (10 µg) and ginseng extract (1, 2, 4, 8, 16, 32, 64 and 128 mg.ml-1). The standard species of C. albicans (PTCC 5027) and C. Krusei (PTCC 5295) were used for the quality control purposes.
Results: The lowest and highest minimum inhibitory concentration (MIC) for C. albicans and C. Kruzei was 0.0625 and 0.5 μg.ml-1, respectively for Itraconazole using the microdilution method. However, the lowest MIC and minimum fungal concentration (MFC) for alcoholic extract was 64 mg.ml-1 .The highest inhibition zone for C. albicans was 14 and 14-32 mm for alcoholic extract and Foritraconazole, respectively. Using the two methods no significant difference was seen between the alcoholic extract of ginseng (64 and 128 mg.ml-1) and the drug. (P<0.05)
Conclusion: Considering the MICs and disk diffusion results, the ginseng extract (64,128 mg.ml-1) shows considerable antifungal effects compared to Itraconazole.
Maryam Kazemi, Gholamreza Ghavipanjeh, Mohammad Esmaeil Shahaboddin, Seyyed Mojtaba Banitaba-Bidgoli ,
Volume 22, Issue 4 (Bimonthly 2018)
Abstract
Background: Matricaria recutit (MAT) has a series of flavonoid compounds with benzodiazepine-like properties. So, it may be effective in the treatment of epilepsy and seizures. This study aimed at examining the effect of intraperitoneal injection of the hydro-alcoholic extract of Matricaria recutita on seizures induced by pentylenetetrazole (PTZ) in mice.
Materials and Methods: In this study, 56 male mice (25-30 g) were divided into seven groups (n=8): 1-control (saline), 2-control (PTZ), 3-MAT (50mg/kg), 4-MAT (100 mg/kg), 5- MAT (200 mg/kg), 6- diazepam (1mg/kg) and 7- MAT (50 mg/kg)+diazepam (0.25 mg/kg). After 10 days of treatment in different groups, the PTZ-induced seizure threshold was assessed and nitric oxide (NOX) metabolites in the brain tissue were measured by the Greiss method.
Results: The chamomile hydro-alcoholic extract (200 mg/kg), and diazepam (1 mg/kg) significantly increased the seizure threshold (P<0.001). The chamomile hydro-alcoholic extract (50, and 100 mg/kg) did not show anticonvulsant activity. The concentrations of NOX in the diazepam (1 mg/kg), MAT 100 mg/kg, and MAT 200 mg/kg groups were significantly lower than those in the control (PTZ) group and co-administration of MAT (50 mg/kg) with diazepam (0.25) decreased the NOX concentration. The hydro-alcoholic extract of MAT (50 mg/kg) had no significant effect on the NOX concentration.
Conclusion: According to the results of this study, the chamomile hydro-alcoholic extract has an inhibitory effect on the PTZ-induced seizure and NOX in male mice.
Masumeh Rezaei, Roghayeh Kamran-Samani, Ahmad Shanei, Mohammad Kazemi, Seyed Hossein Hejazi,
Volume 23, Issue 4 (Bimonthly 2019)
Abstract
Background: Bystander effect is defined as biological responses observed in cells that are not exposed to radiation directly but these radiation effects are induced through messages from irradiated cells. One of the non-invasive methods for cancer treatment is ultrasound waves. The therapeutic efficacy of ultrasound waveslike treatment with ionizing radiation can be altered by the bystander effect, so it is necessary to evaluate the bystander effect after ultrasound exposure. In this study, the effect of ultrasound exposure on the expression of P53 gene in neighboring cells has been investigated.
Materials and Methods: The cells were divided into three groups: control (without treatment), target (under direct ultrasound exposure) and the bystander (no radiation, only receiving target cells culture media). In the target group, the cells were exposed to the ultrasound and after one hour their culture medium were transferred to the bystander group. Cell viability and the expression of P53 gene by using the MTT assay and Real-time PCR was analyzed.
Results: The percentage of cell survival in the target group was reduced compared to the control group, but in the bystander group, this difference was not significant. The expression of P53 gene in the bystander group was statistically increased compared to the control group.
Conclusion: The bystander effect is induced after ultrasound exposure and it can increase the level of P53 gene expression in bystander cells.
Seyyed Vali Kazemi-Rezaei, Seyyed Ali Kazemi-Rezaei,
Volume 24, Issue 2 (Bimonthly 2020)
Abstract
Background: In the contemporary world most people's lives have been affected by modern communication technologies such as mobile phones and computers. The entrance of mobile phones into daily communication and usage has led to new changes in medical and health perspectives. In fact, “Text Neck Syndrome" is a worrying situation and has the potential to affect millions of people around the world.
Materials and Methods: The search articles was done in databases PubMed, Science Direct, Google Scholar and due to the widespread use and prevalence of Smartphone use in the past decade, articles about the years 2010 up to 2019 were extracted. To extract the articles, was used from the related keyword. Then, considering the main subject of the present study and criteria inclusion and exclusion, 10 articles were selected for final investigation.
Results: After reviewing the full text of 10 articles related to the study subject that were chosen for the final review, the results of those articles are described briefly in Table 1 and completely in the discussion section. There is a direct relationship between the duration of phone calls, playing with mobile phones, browsing and the number of texting during the day and musculoskeletal disorders.
Conclusion: The text neck syndrome can cause forward head and rounded shoulder postures, as well as chronic pain in the neck, shoulders, hands, and fingers.
Azar Kazemi, Mohsen Golparvar, Hamid Atashpour,
Volume 24, Issue 2 (Bimonthly 2020)
Abstract
Background: Training the skills which increase positive affect leading to a better problem solving, increase social support and has some other advantages which act as a protective factor thus decreasing depression. So this study aimed to compare the effect of mindful self-acceptance therapy and positive psychotherapy on affective capital of female students with depression.
Materials and Methods: This research was a semi-experimental study pre-test, post-test and one and a half month follow-up design with three groups. The sample consisted of 54 female students with depression at secondary high school in Isfahan who were selected based on the inclusion criteria and were randomly assigned in two experimental groups and one control group. The experimental groups were treated with mindful self-acceptance therapy and positive psychotherapy. Golparvar affect capacity questionnaire were used to measure the dependent variables at pre-test, post-test and the follow up. The data were analyzed using repeated measure analysis of variance and Bonferroni post hoc test trough SPSS-22 software.
Results: The results showed that mindful self-acceptance therapy and positive psychotherapy had a significant effect on increasing the affective capital (P<0.05). But there was no significant difference between the two treatment groups (P>0.05).
Conclusion: Mindful self-acceptance therapy and positive psychotherapy were both effective treatments on increasing the affective capital of female students with depression and training them both can change female student’s sentiments about life thus treating depression as a consequence.
Salman Zarei, Ahmad Kazemipour,
Volume 25, Issue 1 (Bimonthly 2021)
Abstract
Background: Due to the increasing rate of addiction and its destructive consequences, recognizing the factors that contribute to addiction tendency among adolescents can be a useful step toward prevention of addiction. Therefore, this present study aimed to investigate the moderating role of religious well-being and self-differentiation in the relationship between boredom and addiction tendency.
Materials and Methods: The research design was correlational. The statistical population was high school male students in Nourabad at the academic year 1397-1398 that a total of 360 students were selected by cluster sampling. Data were collected using multi-dimensional religious well-being inventory, multidimensional scale of boredom, differentiation of self-inventory-revised, and addiction proneness scale. Then, collected data were analyzed using Pearson correlation and hierarchical linear regression.
Results: The results showed a positive and significant relationship between boredom and addiction tendency (r=0.44, P<0.01), a negative and significant relationship between religious well-being and addiction tendency (r=-0.29, P<0.01) and between self-differentiation and addiction tendency (r=-0.38, P<0.01). Also, based on hierarchical regression’ results the moderating role of religious well-being (β=0.16, P<0.01) and self-differentiation (β=0.25, P<0.01) in the relationship between boredom and addiction tendency was confirmed.
Conclusion: The present provided evidence for the moderating role of religious well-being and self-differentiation in the relationship between boredom and addiction proneness. Therefore, developing religious well-being and training self-differentiation among adolescents could be a good way to protect them in the face of boredom and addiction tendency.
Fatemeh Rangrazian, Azar Kiamarsi, Reza Kazemi,
Volume 25, Issue 2 (Bimonthly 2021)
Abstract
Abstract
Introduction Schizophrenia is a chronic and debilitating disorder in which communication skills are severely impaired. The aim of this study was to compare the effectiveness of supportive therapy and illness management on communication skills of schizophrenic patients.
Methods: This quasi-experimental study was performed with a post-test pretest design with a control group. The sample consisted of 30 patients with schizophrenia who were randomly selected from among patients with schizophrenia admitted to Shafa Hospital in Rasht in 2019 and were divided into 3 groups of 10 (two experimental groups and one control group). The communication skills questionnaire and Christie Forsyth (1996) interactive questionnaire before and after the training were used to collect data. Supportive therapy and illness management were performed in groups on each of the experimental groups and during this period the control group did not receive any intervention. Research data were analyzed by Kruskal-Wallis and Uman-Witni tests
Results: The results showed that supportive therapy and illness management had an effect on increasing communication skills. But there was no difference between the effectiveness of supportive therapy and illness management in communication skills (P <0.01).
Conclusions: These findings indicate that supportive therapy and illness management can be used to improve the quality of life of schizophrenic patients along with other treatments or in combination.
Dr. Alireza Shoae Hassani, Dr. Mahdi Shadnoush, Dr. Hadi Kazemi, Dr. Nasim Vosughi, Dr. Rashin Mohseni, Dr. Iman Seihoon, Dr. Javad Verdi,
Volume 25, Issue 3 (Bimonthly 2021)
Abstract
Background: Neuroblastoma (NB) is one of the most prevalent children's neoplasm and some of which still do not respond to common treatments and is an ideal candidate for NK cell immune therapy. In this research, we have performed cytokine activation of NKs by using proper interleukins to obtain more cytolysis which was evaluated in vitro and in vivo.
Materials and Methods: Isolation of NKs from human peripheral blood was performed using CD56 marker via magnetic activated cell sorter (MACS). The IL-2 and IL-15 cytokines were used for proliferation, and IL-21 for activation of cells. The neuroblastoma model was developed using the SK-N-SH cell line in Nude mice. The in vitro effect of the NKs on the two neuroblastoma lines were assessed after activation and via flow cytometry confirmation.
Results: The results showed that at the ratio of 1:10 the IL-21 activated NKs lysed 94% of the SK-N-SH and 91% of the CHLA-255 cell lines (P<0.05), in spite of the only 40% and 38% of lysis for these cell lines without activation (P<0.05), respectively. The NKs activated by IL-21 were able to eliminate 70% (P<0.05) of the xenograft neuroblastoma tumors in nude mice. Conclusion: The present investigation was showed that the activation of NK cells using IL-21 can be useful in the treatment of neuroblastoma and could be applied in future clinical trials.
Mohammad Kazemipour, Hasan Mateen Homaie, Parvin Farzanegi,
Volume 26, Issue 5 (Bimonthly 2022)
Abstract
Background: Fatty diet increases apoptosis and cardiovascular disease. Therefore, this study aimed to evaluate the effect of 4-week aerobic exercise along with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats.
Materials and Methods: This is an experimental study. 12-week-old female rats were randomly divided into 5 groups of 6; Positive control (healthy), negative control (obesity), exercise + obesity, extract + obesity, exercise and extract + obesity (interaction of exercise and extract). Training was done for 4 weeks with moderate intensity of 50-60% Vo2max. Pistachio skin extract was used at a dose of 60 mg per kilogram. qPCR method was used to check gene expression.
Results: The results showed that the expression of bax and caspase 3 was higher in the obese group than in the healthy group (P=0.001). The extract groups, the exercise group and the exercise + extract group had more bax and caspase 3 expression compared to the obese group (P=0.001). The expression of Bcl-2 in the obese group and the exercise group was lower than the healthy group (P=0.001). Also, the expression of Bcl-2 was higher in the exercise + extract group than in the obese group (P=0.001). There was a significant difference between the exercise group and the exercise+extract group with the extract group.
Conclusion: High-fat diet increase bax and caspase 3 and decreased Bcl-2 in rats. However, exercise and consumption of green pistachio skin extract together decrease bax and caspase 3 and increased Bcl-2.
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