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Showing 4 results for Alimoradi
Mohammad Fathizadeh, Sahar Alimoradi,
Volume 24, Issue 4 (Bimonthly 2020)
Abstract
Background: In patients with subclinical hypothyroidism the levels of cortisol decrease and the levels of glucose and insulin resistance increase. Therefore, the aim of this study was to investigate the effect of 5 months aerobic exercise training with and without Levothyroxine on serum cortisol, glucose, insulin and insulin resistance in subclinical hypothyroidism women.
Materials and Methods: In this semi-experimental study, after obtaining written consent, 25 subclinical hypothyroidism women were divided into three groups: drug-exercise (n=8), exercise (n=7), and drug (n=10). Aerobic exercise training consisted of 3 sessions per week for 5 months with 50% maximal heart rate in the first week and 75% maximal heart rate in the last week. The drug group was inactive persons who took only one dose of 0.1 mg sodium Levothyroxine tablets daily. Serum levels of cortisol, fasting plasma sugar, insulin and insulin resistance were measured in three groups before and after 5 months. Data were analyzed using analysis of MANOVA.
Results: The results of MANOVA and intergroup analysis showed that there was no significant difference between the three groups in the levels of plasma sugar (P=0.273), insulin (P=0.062), insulin resistance (P=0.070) and cortisol (P=0.123).
Conclusion: It seems that 5 months of aerobics exercise with and without Levothyroxine leads to non-significant changes in serum cortisol, plasma sugar, insulin and insulin resistance in subclinical hypothyroidism women.
Arash Alimoradi, Hasan Mateen Homaie, Saleh Rahmati,
Volume 26, Issue 1 (Bimonthly 2022)
Abstract
Background: There is a close link between eating fatty foods, the prevalence of obesity and type 2 diabetes. This study aimed to evaluation the effect of 8 weeks caloric restriction on Perilipins (PLIN5) and ATGL visceral adipose tissue and insulin resistance in male diabetic rats.
Material and Methods: In this experimental study, 24 male rats were divided into healthy control, diabetic control and diabetic group with caloric restriction. In the caloric restriction group, %40 of the calorie intake was restricted and other groups received the standard food they needed freely for 8 week. The relative expression of prilipine 5 and ATGL protein by Western blotting and insulin resistance were also evaluated using Homa index. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Result: A significant difference was showed between the means of the three groups in comparing the expression of PLIN5 and ATGL proteins. Post hoc test showed a significant increase in PLIN5 and ATGL in the caloric restriction group compared to the healthy and diabetic control group (P=0.001). There was also a significant decrease in insulin and glucose levels in the caloric restriction group compared to the diabetic control group (p = 0.001).
Conclusion: Calorie restriction may prevent insulin resistance by acting on the overlying proteins and thus reducing fat accumulation.
Arash Alimoradi, Hasan Mateen-Homaie, Saleh Rahmati,
Volume 26, Issue 4 (Bimonthly 2022)
Abstract
Background: There is ample evidence in the study of the association of inflammatory reactions with the pathogenesis and complications of diabetes, especially hyperlipidemia and atherosclerosis. This study aimed to the effect of high intensity interval training and caloric restriction on inflammatory factors and PLIN3 visceral adipose tissue in male diabetic rats.
Materials and Methods: 32 diabetic male Wistar rats was randomly divided into three subgroups: HIIT, control and caloric restriction. In the caloric restriction group, 40% of the calorie intake was restricted and other groups received the standard food they needed freely. Relative protein expression of PLIN3 was performed using western blot technique and TNF-α and IL-6 by ELISA. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Result: There was a significant difference between the mean of PLIN3, IL-6 and TNF-α in the three groups (P=0.001). The results of post hoc test showed a significant increase in PLIN3 and a significant decrease in IL-6 and TNF-α in the caloric restriction group and intense intermittent exercise compared to the diabetic control group (P=0.001).
Conclusion: HIIT and caloric restriction may increase lipid oxidation by increasing PLIN3 expression. Considering the role of TNF-α and IL-6 in increasing insulin resistance and type 2 diabetes, reducing these factors during the present study may be helpful in preventing these metabolic diseases.
Mozhdeh Safari, Robab Rafiei Tabatabaei, Hamid Abtahi, Shohreh Fahimirad, Abbas Alimoradian,
Volume 28, Issue 1 (Bimothly 2024)
Abstract
Background and Aim: Multi-drug resistant (MDR) Acinetobacter baumannii is a prevalent hospital pathogen. Antimicrobial peptides (AMPs), such as Oncorhyncin II, have been proposed for treating MDR pathogens. This study aimed to assess the antibacterial activity of recombinant protein Oncorhyncin II against Acinetobacter baumannii (ATCC19606) under in vitro conditions.
Methods: In this experimental study, the gene construct pET32a-oncorhyncin II was transferred to Escherichia coli BL21 (DE3) for expressing the recombinant Oncorhyncin II protein. Purification was optimized using nickel affinity chromatography (Ni-NTA), and the peptide's efficacy was evaluated by determining the minimum inhibitory concentration (MIC), bactericidal kinetics, and growth kinetics against Acinetobacter baumannii (ATCC19606).
Results: The recombinant Oncorhyncin II protein was successfully expressed in E. coli BL21 (DE3) and purified with over 70% purity. The MIC assay confirmed effective antibacterial activity of Oncorhyncin II against Acinetobacter baumannii at a concentration of 95.87 μg/ml. At 2x MIC, Oncorhyncin II led to a rapid reduction in viable cell count and decreased turbidity of the bacterial inoculum in bactericidal and growth kinetics assessments.
Conclusion: The produced recombinant Oncorhyncin II exhibited potent antibacterial activity against Acinetobacter baumannii. These findings can contribute to the development and production of novel antimicrobial drugs for treating infections caused by Acinetobacter baumannii.
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