:: Volume 12, Issue 4 (Quaterly 2009) ::
Feyz 2009, 12(4): 13-21 Back to browse issues page
Histopathological study of the effects of ibuprofen on gastric surgical trauma in rats
Dariush Mohajeri *, Ghafour Mousavi, Mir Hadi Khayat Noori, Ali Asefi
, daryoushmohajeri@yahoo.com
Abstract:   (8667 Views)

Background : Nowadays faster healing of surgical wounds to decrease wound complications, especially pain, is a matter of challenge for researchers. Synthetic drugs are more effective for the improvement of wound healing. The main objective of the present study was to evaluate the healing effects of ibuprofen, as a non-selective COX-1 and COX-2 inhibitor, on surgical trauma of stomach in rats.

Materials and Methods : In this experimental study fifteen Wistar rats were randomly assigned into three groups of five animals . To induce trauma, a gastrotomy incision with a width of 1 cm was made in the grater curvature of rat stomach. Thereafter, it was sutured in 2 layers. After the surgery, the experimental group was treated with daily oral ibuprofen for 15 days in a dose of 30 mg/kg (dissolved in 10 ml/kg of DMSO 5%). The placebo and control groups received DMSO (5%) and saline (10 ml/kg), respectively, in the same manner. Histopathologi cal studies for the evaluation of healing were carried out in experimental rats, which were euthanized 15 days after the experiment using hematoxylin-eosin (H&E) and Masson's trichrome staining methods. Wound healing within the groups was compared with the consideration of more effective agents on wound healing (e.g., proliferation of fibroblasts, angiogenesis , re-epithelialization and collagen synthesis ).Significant differences (p<0.05) among the groups were determined by ANOVA followed by Bonferroni correction.

Results: The numbers of fibroblasts and capillary buds in ibuprofen treated rats were significantly less than those of the healthy control and DMSO control groups (p<0.05). The epithelial gap in treated rats was more than that in the other groups (p<0.05) . There were significant differences in collagen contents of wound healing region between the ibuprofen treated and other experimental groups.

Conclusion: The results of this study indicate that ibuprofen has an inhibitory effect on surgical wound healing of stomach. Therefore, it is not recommended as a postoperative analgesic.

Keywords: Ibuprofen, Wound healing, Stomach, Rat, Histopathology
Full-Text [PDF 792 kb]   (2532 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/05/5 | Revised: 2009/05/9 | Published: 2009/01/15

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Volume 12, Issue 4 (Quaterly 2009) Back to browse issues page