History and Objectives: Much controversy exists regarding the prevalence of Helicobacter pylori in patients with non-ulcer dyspepsia and its complications. Despite the importance of timely diagnosis, there is no consensus on the predictive value of the various methods for identifying this microorganism. All these led us to perform a study on patients with non-ulcer dyspepsia referred to Kashan’s Shaheed Beheshti hospital to determine the prevalence of H.pylori and the diagnostic power of staining with carbol-fuscin or Giemsa, the urea rapid test and bacterial cultures.

Materials and Methods: Using a sample of 107 patients, a descriptive study was performed to determine H.pylori prevalence and a diagnostic experimental trial to measure diagnostic power. Diagnosis of non-ulcer dyspepsia was based on clinical and paraclinical investigation (Including blood cell count, licer, kidney and thyroid function tests, ultrasonography and electrocardiography). A positive culture by calculating each test’s sensitivity, specificity and positive and negative predictive value (PPV and NPV).

Results: H.pylori prevalence in patients with non-ulcer dyspepsia was 47.6%. The PPV of carbol-fuscin staining, urea rapid test and Giemsa staining was 778, 64.2 and 62.9 percent, respectively, with on NPV of 95.5, 82.5 and 81.1%, respectively.

Conclusion: Due to the greater power of carbol-fuscin staining and its simplicity in identifying H.pylori, we suggest this method be used for diagnosis.