Background and Objective The prevalence of musculoskeletal disorders in various medical professions, including anestethia technician and surgical technologist (operating room), requires special attention due to the nature of the tasks and the relatively long time required to perform each task in a static state. The present study was conducted with the aim of investigating such disorders in the mentioned jobs and determining the factors that cause them. Methods This descriptive and analytical study was conducted in 2023 by examining 72 people (including 38 surgical technologist and 34 anestethia technician). Musculoskeletal disorders were assessed using the Nordic Standard Questionnaire, and posture analysis via the Whole Body Posture Assessment (REBA) method. The data was analyzed using SPSS version 23 statistical software. The significance level of all statistical tests was considered less than 0.05. Findings The most cases of musculoskeletal disorders in the last year in the operating room group were seen in the sitting area and upper back (42%), shoulder (29%), neck (24%) and lower back (24%), while in the anestethia technician group, more disorders were seen in neck area (35%), shoulder (25%) and wrists and hands (23%) have been observed. Examining the relationship between the final score of posture assessment and musculoskeletal disorders also showed that with the increase in the posture score, the percentage of people with musculoskeletal disorders increases weekly and annually. Conclusion In general, in anestethia technicians, the main factors of musculoskeletal disorders include inappropriate postures, static work, especially in the masking activity, while in operating room experts, these factors include inappropriate posture, applying excessive force and standing for a long time were identified. On the other hand, the gender, age and work experience, as well as the height of people, played a role in the occurrence of musculoskeletal disorders and their higher prevalence.