History and Objectives: Kanamycin is an amino glycoside that was widely administered in the treatment of urinary tract infections in the past, but it is no longer used at present because of resistance to it. After several years the Kanamycin has not been used, this study was performed to determine sensitivity of bacterial agents urinary tract infections to Kanamycin and other commonly used antibiotics.

Materials and Methods: A descriptive study was performed on patients with urinary tract infection hospitalized in the ward of infections diseases. A urine culture on blood agar and EMB was performed for all patients. Differential culture media were used to diagnose bacterial type. Then transfer on TSB liquid culture media has been done and Mueller Hinton culture medium was used for antibiogram and discs of ampicillin, co-trimoxazole, cephalothin, ceftizoxime, tobramycin and Kanamycin were applied. Results of antibiogram were reported as sensitive or resistant and then data were analyzed.

Results: In 157 patients with positive urine cultures and mean age of 53.6 years, bacterial sensitivity to ampicillin, co-trimoxazole, cephalothin, tobramycin and Kanamycin was 17.27, 38.77, 47.17, 61.97 and 71.27 respectively.

Conclusion: Sensitivity of etiologic bacterial agents of urinary tract infection is most to kanamycin. A clinical trial experimental study to compare the therapeutic effects of this antibiotic is recommended.