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:: Volume 3, Issue 4 (Quarterly 2000) ::
Feyz 2000, 3(4): 30-37 Back to browse issues page
Accuracy of Whole-Cell ELISA and HM-Cap ELISA in the diagnosis of Helicobacter Pylori infection
Ali Fazeli *, Mohamamd Reza Nafisi, Kambiz Hazeghi, Ahmad Ghavaminejad
Abstract:   (15147 Views)

History and Objectives: Due to usage of multiple methods in the diagnosis of Helicobacter Pylori and considering the advantage of noninvasive modalities including serologic examination and various reports on the diagnostic accuracy of these tests and in order to determine the sensitivity and the specificity Whole-Cell ELISA and HM-Cap ELISA, this study was performed on the referrers to the ward of endoscopy of Alzahra and Fayz hospitals of Isfahan in 1375.

Materials and Methods: A clinical trial study was performed on 168 patients with the complaint of upper gastrointestinal tract and indication for endoscopy. Sex, age and clinical manifestations of the patients were recorded on the data sheets. After performing endoscopy, the specimen obtained by biopsy was sent to laboratory for microbiologic and histologic examinations and four milliliters venous blood was obtained from each individual in order to perform morphologic evaluation. The results of culture, histologic and serologic examinations were recorded on the data sheets. The data on the sheets were classified and sensitivity, specificity, positive and negative predictive values and general efficacy of the serologic examinations, in contrast with the gold standard (Culture and histology) was determined.

Results: From the 168 patients under study, 83 individuals (49.4%) were female and 85 (50.6%) were male with the mean age of 44.8 years. From the whole individuals under study 123 patients (73.2%) had positive results for Helicobacter pylori. Helicobacter pylori was most commonly (42.2%) observed in the 40 to 60 year-old age group. The most common cause (47%) was gastritis. Sensitivity and specificity and general efficacy were 89.4%, 75.6% and 85.7% for the Whole-Cell ELISA respectively and 95.1%, 93.3% and 94.6% for HM-Cap ELISA respectively.

Conclusion: HM-Cap ELISA is more valuable in the diagnosis of Helicobacter pylori and considering its ease of performance and its low cost, is recommended for usage.

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Type of Study: Research | Subject: General
Received: 2008/11/17 | Published: 2000/01/15
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Fazeli A, Nafisi M R, Hazeghi K, Ghavaminejad A. Accuracy of Whole-Cell ELISA and HM-Cap ELISA in the diagnosis of Helicobacter Pylori infection . Feyz. 2000; 3 (4) :30-37
URL: http://feyz.kaums.ac.ir/article-1-477-en.html

Volume 3, Issue 4 (Quarterly 2000) Back to browse issues page
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