:: Volume 26, Issue 6 (Bimonthly 2022) ::
Feyz 2022, 26(6): 646-656 Back to browse issues page
The effect of high-fat diet and continuous endurance training on expression of TFEB and E2F1 transcription factors in visceral adipose tissue of mice
Saeed Daneshyar , Zahra Mirakhori , Yazdan Forutan
, s.daneshyar@abru.ac.ir
Abstract:   (807 Views)
Background: Transcription Factor EB (TFEB) and Transcription Factor E2F1 play an important role in metabolism. This study investigated the effect of exercise training and high-fat diet on the gene expression of these factors in the visceral adipose tissue of mice.
Materials and Methods: In the experimental study, 28 male mice (C57BL/6) were assigned into four groups included Control, High-Fat Diet (HFD), Exercise Training (ET), and High-Fat Diet with Exercise Training (HFD-ET). The subjects of HFD were fed a high-fat diet for 12 weeks. The mice of ET performed continuous endurance training on a treadmill for six weeks. The mice of HFD-ET had six weeks of endurance training in addition to having the HFD. The Real-Time–PCR methods were used to measure the gene expression of TFEB and E2F1.
Results: 1) The gene expression of TFEB was increased by HFD and ET (P<0.05). 2) The combination of HFD and ET had an increasing effect on TFEB (P=0.02); However, this effect was not higher than ET and HFD, separately (P>0.05). 3) HFD caused an increase in E2F1 (P=0.03). 4) Neither ET nor combined HFD and ET significantly increased the expression of E2F1 (P>0.05).
Conclusion: Continuous endurance training has a similar effect (no opposite effect) to the high-fat diet on gene expression of TFEB. Further, it could be thought that this exercise training may partly negate the increasing effect of the high-fat diet on E2F1 expression.  
Keywords: High-fat diet, Exercise training, Endurance training, Adipose tissue, TFEB, E2F1
Full-Text [PDF 573 kb]   (303 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2022/08/4 | Revised: 2023/02/20 | Accepted: 2022/11/7 | Published: 2023/02/22
References
1. Caudwell P, Gibbons C, Finlayson G, Näslund E, Blundell J. Physical Activity, Energy Intake, and Obesity: The Links Between Exercise and Appetite. Curr Obes Rep 2013; 2(2): 185-90.
2. Cummins TD, Holden CR, Sansbury BE, Gibb AA, Shah J, Zafar N, et al. Metabolic remodeling of white adipose tissue in obesity. Am J Physiol Endocrinol 2014; 307(3): E262-77.
3. He MQ, Wang JY, Wang Y, Sui J, Zhang M, Ding X, et al. High-fat diet-induced adipose tissue expansion occurs prior to insulin resistance in C57BL/6J mice. Chronic Dis Transl Med 2020; 6(3): 198-207.
4. van der Heijden RA, Sheedfar F, Morrison MC, Hommelberg PP, Kor D, Kloosterhuis NJ, et al. High-fat diet induced obesity primes inflammation in adipose tissue prior to liver in C57BL/6j mice. Aging 2015; 7(4): 256-68.
5. McKie GL, Wright DC. Biochemical adaptations in white adipose tissue following aerobic exercise: from mitochondrial biogenesis to browning. Biochem J 2020; 477(6): 1061-81.
6. Gollisch KS, Brandauer J, Jessen N, Toyoda T, Nayer A, Hirshman MF, et al. Effects of exercise training on subcutaneous and visceral adipose tissue in normal- and high-fat diet-fed rats. Am J Physiol Endocrinol 2009; 297(2): E495-504.
7. Rocha-Rodrigues S, Rodríguez A, Gonçalves IO, Moreira A, Maciel E, Santos S, et al. Impact of physical exercise on visceral adipose tissue fatty acid profile and inflammation in response to a high-fat diet regimen. Int J Biochem Cell Biol 2017; 87: 114-24.
8. Napolitano G, Ballabio A. TFEB at a glance. J Cell Sci 2016; 129(13): 2475-81.
9. Settembre C, De Cegli R, Mansueto G, Saha PK, Vetrini F, Visvikis O, et al. TFEB controls cellular lipid metabolism through a starvation-induced autoregulatory loop. Nat Cell Biol 2013; 15(6): 647-58.
10. Salma N, Song JS, Kawakami A, Devi SP, Khaled M, Cacicedo JM, et al. Tfe3 and Tfeb transcriptionally regulate peroxisome proliferator-activated receptor γ2 expression in adipocytes and mediate adiponectin and glucose levels in mice. Mol Cell Biol 2017; 37(15): e00608-16.
11. Denechaud PD, Fajas L, Giralt A. E2F1, a novel regulator of metabolism. Front Endocrinol 2017; 8: 311.
12. Xiong M, Hu W, Tan Y, Yu H, Zhang Q, Zhao C, et al. Transcription Factor E2F1 Knockout Promotes Mice White Adipose Tissue Browning Through Autophagy Inhibition. Front Physiol 2021; 12.
13. Kim J, Kim SH, Kang H, Lee S, Park SY, Cho Y, et al. TFEB–GDF15 axis protects against obesity and insulin resistance as a lysosomal stress response. Nat Metab 2021; 3(3): 410-27.
14. Choi Y, Jang S, Choi M-S, Ryoo ZY, Park T. Increased expression of FGF1-mediated signaling molecules in adipose tissue of obese mice. J Physiol Biochem 2016; 72(2): 157-67.
15. Haim Y, Blüher M, Slutsky N, Goldstein N, Klöting N, Harman-Boehm I, et al. Elevated autophagy gene expression in adipose tissue of obese humans: a potential non-cell-cycle-dependent function of E2F1. Autophagy 2015; 11(11): 2074-88.
16. Shirkhani S, Marandi SM, Kazeminasab F, Esmaeili M, Ghaedi K, Esfarjani F, et al. Comparative studies on the effects of high-fat diet, endurance training and obesity on Ucp1 expression in male C57BL/6 mice. Gene 2018; 676: 16-21.
17. Arras M, Autenried P, Rettich A, Spaeni D, and Rülicke T. Optimization of intraperitoneal injection anesthesia in mice: drugs, dosages, adverse effects, and anesthesia depth. Comp Med 2001; 51(5): 443-56.
18. Teixeira-Coelho F, Fonseca CG, Barbosa NHS, Vaz FF, Cordeiro LMdS, Coimbra CC, et al. Effects of manipulating the duration and intensity of aerobic training sessions on the physical performance of rats. PloS One 2017; 12(8): e0183763.
19. Høydal MA, Wisløff U, Kemi OJ, and Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil 2007; 14(6): 753-760.
20. Wang Y, Wisloff U, Kemi OJ. Animal models in the study of exercise-induced cardiac hypertrophy. Physiol Res 2010; 59(5): 633.
21. Deng Y, Xu J, Zhang X, Yang J, Zhang D, Huang J, et al. Berberine attenuates autophagy in adipocytes by targeting BECN1. Autophagy 2014; 10(10): 1776-86.
22. Chao X, Wang S, Yang L, Ni HM, and Ding WX. Trehalose activates hepatic transcription factor EB (TFEB) but fails to ameliorate alcohol‐impaired TFEB and liver injury in mice. Alcohol Clin Exp Res 2021; 45(10): 1950-64.
23. Flowers S, Xu F, and Moran E. Cooperative activation of tissue-specific genes by pRB and E2F1. Cancer research 2013; 73(7): 2150-8.
24. Parousis A, Carter HN, Tran C, Erlich AT, Mesbah Moosavi ZS, Pauly M, et al. Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells. Autophagy 2018; 14(11): 1886-97.
25. Castro G, Areias MF, Weissmann L, Quaresma PGF, Katashima CK, Saad MJA, et al. Diet-induced obesity induces endoplasmic reticulum stress and insulin resistance in the amygdala of rats. FEBS Open Bio 2013; 3: 443-9.
26. Ferhat M, Funai K, Boudina S. Autophagy in adipose tissue physiology and pathophysiology. Antioxid Redox Signal 2019; 31(6): 487-501.
27. Erlich AT, Brownlee DM, Beyfuss K, and Hood DA. Exercise induces TFEB expression and activity in skeletal muscle in a PGC-1α-dependent manner. Am J Physiol, Cell Physiol 2018; 314(1): C62-C72.
28. Steinberg GR, Carling D. AMP-activated protein kinase: the current landscape for drug development. Nat Rev Drug Discov 2019; 18(7): 527-51.
29. Paquette M, El-Houjeiri L, L CZ, Puustinen P, Blanchette P, Jeong H, et al. AMPK-dependent phosphorylation is required for transcriptional activation of TFEB and TFE3. Autophagy 2021; 17(12): 3957-3975.
30. Evans TD, Zhang X, Jeong SJ, He A, Song E, Bhattacharya S, et al. TFEB drives PGC-1α expression in adipocytes to protect against diet-induced metabolic dysfunction. Sci Signal 2019; 12(606): eaau2281.
31. Wang S, Chen Y, Li X, Zhang W, Liu Z, Wu M, et al. Emerging role of transcription factor EB in mitochondrial quality control. Biomed Pharmacother 2020; 128: 110272.
32. Mansueto G, Armani A, Viscomi C, D'Orsi L, De Cegli R, Polishchuk EV, et al. Transcription Factor EB Controls Metabolic Flexibility during Exercise. Cell Metab 2017; 25(1): 182-96.
33. Nautiyal J, Christian M, Parker MG. Distinct functions for RIP140 in development, inflammation, and metabolism. Trends Endocrinol Metab 2013; 24(9): 451-9.
34. Włodarczyk M, Nowicka G. Obesity, DNA damage, and development of obesity-related diseases. Int J Mol Sci 2019; 20(5): 1146.
35. Stevens C, Thangue NB. The emerging role of E2F-1 in the DNA damage response and checkpoint control. DNA Repair 2004; 3(8-9): 1071-9.
36. Barzegari A, Kazari S, Satvati Niri Z, Alizadeh Mirashrafi MA. Comparison of the effect of different intensities of aerobic exercise on the expression of transcription factors E2F1 and E2F4 in liver tissue of Wistar rats. RJMS 2022; 29(1): 48-59. [in Persian]
37. Radák Z, Naito H, Kaneko T, Tahara S, Nakamoto H, Takahashi R, et al. Exercise training decreases DNA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle. Pflügers Arch 2002; 445(2): 273-8.
38. Soares JP, Silva AM, Oliveira MM, Peixoto F, Gaivão I, Mota MP. Effects of combined physical exercise training on DNA damage and repair capacity: role of oxidative stress changes. Age 2015; 37(3): 1-12.
39. Setayesh T, Mišík M, Langie SAS, Godschalk R, Waldherr M, Bauer T, et al. Impact of Weight Loss Strategies on Obesity-Induced DNA Damage. Mol Nutr Food Res 2019; 63(17): e1900045.



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Volume 26, Issue 6 (Bimonthly 2022) Back to browse issues page