:: Volume 26, Issue 6 (Bimonthly 2022) ::
Feyz 2022, 26(6): 646-656 Back to browse issues page
The effect of high-fat diet and continuous endurance training on expression of TFEB and E2F1 transcription factors in visceral adipose tissue of mice
Saeed Daneshyar , Zahra Mirakhori , Yazdan Forutan
, s.daneshyar@abru.ac.ir
Abstract:   (807 Views)
Background: Transcription Factor EB (TFEB) and Transcription Factor E2F1 play an important role in metabolism. This study investigated the effect of exercise training and high-fat diet on the gene expression of these factors in the visceral adipose tissue of mice.
Materials and Methods: In the experimental study, 28 male mice (C57BL/6) were assigned into four groups included Control, High-Fat Diet (HFD), Exercise Training (ET), and High-Fat Diet with Exercise Training (HFD-ET). The subjects of HFD were fed a high-fat diet for 12 weeks. The mice of ET performed continuous endurance training on a treadmill for six weeks. The mice of HFD-ET had six weeks of endurance training in addition to having the HFD. The Real-Time–PCR methods were used to measure the gene expression of TFEB and E2F1.
Results: 1) The gene expression of TFEB was increased by HFD and ET (P<0.05). 2) The combination of HFD and ET had an increasing effect on TFEB (P=0.02); However, this effect was not higher than ET and HFD, separately (P>0.05). 3) HFD caused an increase in E2F1 (P=0.03). 4) Neither ET nor combined HFD and ET significantly increased the expression of E2F1 (P>0.05).
Conclusion: Continuous endurance training has a similar effect (no opposite effect) to the high-fat diet on gene expression of TFEB. Further, it could be thought that this exercise training may partly negate the increasing effect of the high-fat diet on E2F1 expression.  
Keywords: High-fat diet, Exercise training, Endurance training, Adipose tissue, TFEB, E2F1
Full-Text [PDF 573 kb]   (303 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2022/08/4 | Revised: 2023/02/20 | Accepted: 2022/11/7 | Published: 2023/02/22
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Volume 26, Issue 6 (Bimonthly 2022) Back to browse issues page