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:: Volume 27, Issue 2 (Bimonthly 2023) ::
Feyz 2023, 27(2): 154-163 Back to browse issues page
The effect of insulin-like growth factor-1 on the proliferation of goat spermatogonial stem cells (SSCs) in vitro
Milad Torabi , Peyman Rahimi-Feyli , Aliasghar Moghaddam
Department of Clinical Sciences, Faculty of Veterinary Medicine, I.R. Iran. , drp.rahimi@gmail.com
Abstract:   (577 Views)
Background: This study aimed to the effect of insulin-like growth factor-1 on the proliferation of goat spermatogonial stem cells (SSCs) in vitro.
Materials and Methods: The testicles of immature goats were obtained from slaughterhouse. Isolation of spermatogonial cells was performed by two-step digestion. The isolated cells were cultured in 4 different groups: 3 groups by adding different doses of IGF-1 (10, 40 and 100 ng/ml) to the basic medium (DMEM containing 5% fetal bovine serum and 1% antibiotic) and the control group (without IGF-1). Data were analyzed by one-way ANOVA and Tukey test at 5% level. Presence of SSCs at the end of culture was determined by reverse transcription polymerase chain reaction (RT-PCR) for several important spermatogonial markers (PGP9.5, THY-1, PLZF, Oct4 and VASA) and immunohistochemical staining against PGP9.5 antigen.
Results: In this study, the number of spermatogonial cell colonies in treatment 3 was significantly higher than the control and other treatment groups on days 4, 7, and 10 of culture (P<0.05). Also, the surface area of G10 colonies on days 7 and 10 of culture was significantly different (P<0.05). The surface area of G100 on days 4, 7, and 10 after culture was significantly higher than control, G10 and G40 (P<0.05). The isolated cells expressed all surface markers related to stem cells.
Conclusion: According to this results, the addition of 100 ng/ml IGF-1 to culture medium for promoting colonization of goat Spermatogonial stem cells is recommended.
Keywords: Insulin-like growth factor-1, stem cells, Goat, Spermatogonia, RT-PCR
Full-Text [PDF 470 kb]   (299 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/05/1 | Revised: 2023/07/25 | Accepted: 2023/05/10 | Published: 2023/06/14
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Torabi M, Rahimi-Feyli P, Moghaddam A. The effect of insulin-like growth factor-1 on the proliferation of goat spermatogonial stem cells (SSCs) in vitro. Feyz 2023; 27 (2) :154-163
URL: http://feyz.kaums.ac.ir/article-1-4603-en.html


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Volume 27, Issue 2 (Bimonthly 2023) Back to browse issues page
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