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:: Volume 26, Issue 1 (Bimonthly 2022) ::
Feyz 2022, 26(1): 47-54 Back to browse issues page
The effect of 8 weeks caloric restriction on PLIN5 and ATGL visceral adipose tissue and insulin resistance in type 2 diabetic male rats
Arash Alimoradi , Hasan Mateen Homaie * , Saleh Rahmati
Department of Exercise Physiology٫ Faculty of Physical Education and Sport Sciences٫ Central Tehran Branch٫ Islamic Azad University٫ Tehran٫ I.R. Iran. , hasanmatinhomaee@gmail.com
Abstract:   (292 Views)
Background: There is a close link between eating fatty foods, the prevalence of obesity and type 2 diabetes. This study aimed to evaluation the effect of 8 weeks caloric restriction on Perilipins (PLIN5) and ATGL visceral adipose tissue and insulin resistance in male diabetic rats.
Material and Methods: In this experimental study, 24 male rats were divided into healthy control, diabetic control and diabetic group with caloric restriction. In the caloric restriction group, %40 of the calorie intake was restricted and other groups received the standard food they needed freely for 8 week. The relative expression of prilipine 5 and ATGL protein by Western blotting and insulin resistance were also evaluated using Homa index. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Result: A significant difference was showed between the means of the three groups in comparing the expression of PLIN5 and ATGL proteins. Post hoc test showed a significant increase in PLIN5 and ATGL in the caloric restriction group compared to the healthy and diabetic control group (P=0.001). There was also a significant decrease in insulin and glucose levels in the caloric restriction group compared to the diabetic control group (p = 0.001).
Conclusion: Calorie restriction may prevent insulin resistance by acting on the overlying proteins and thus reducing fat accumulation.
Keywords: Caloric restriction, Perilipins, Adipose triglyceride lipase, Insulin resistance
Full-Text [PDF 371 kb]   (123 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/01/3 | Accepted: 2022/03/6 | Published: 2022/04/4
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Alimoradi A, Mateen Homaie H, Rahmati S. The effect of 8 weeks caloric restriction on PLIN5 and ATGL visceral adipose tissue and insulin resistance in type 2 diabetic male rats. Feyz. 2022; 26 (1) :47-54
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