:: Volume 26, Issue 1 (Bimonthly 2022) ::
Feyz 2022, 26(1): 75-81 Back to browse issues page
Association of -308G/A TNFα gene polymorphism with reduced risk of idiopathic infertility in men
Tayyebeh Zamani-Badi , Hossein Nikzad , Atieh Rafatmanesh , Zeinab Rezazadeh Lavaf , Mohammad Karimian
University of Mazandaran , mdkarimian@gmail.com
Abstract:   (1073 Views)
Background: Infertility is one of the major health problems in the world and several factors play a role in the occurrence of this complication. One of the causes of idiopathic male infertility is a defect in the process of spermatogenesis due to genetic changes in cytokines involved in this process. Tumor Necrosis Factor-alpha (TNFα) as a multifunctional cytokine controls spermatogenesis-related cellular activity. In this study, the association of -308G/A polymorphism in the TNFα gene with male infertility was investigated.
Materials and Methods: In a case-control study, blood samples were collected from 82 infertile men and 107 fertile men. After DNA extraction, the genotype of the samples at the -308G/A region was determined using the PCR-RFLP technique.
Results: Data analysis showed a significant association between GA genotype and reduced risk of male infertility. Also, in the subgroup study, a significant association was observed between this genotype and the reduction of oligozoospermia and asthenozoospermia risk. Similar results were found for the association of carriers of allele A (GA + AA) and idiopathic male infertility. In addition, the allelic analysis showed a significant association between allele and a reduced risk of idiopathic male infertility. Subgroup analysis showed a significant association between this allele and reduced risk of asthenozoospermia, also.
Conclusion: Based on findings of this research, the TNFα -308G/A polymorphism can be considered as a protective factor and a potential biomarker for idiopathic male infertility.
Keywords: Male infertility, -308G/A polymorphism, TNFα gene, Cytokine, PCR-RFLP
Full-Text [PDF 711 kb]   (743 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/09/8 | Revised: 2022/04/17 | Accepted: 2022/02/13 | Published: 2022/04/4
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