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:: Volume 23, Issue 6 (Bimonthly 2019) ::
Feyz 2019, 23(6): 596-604 Back to browse issues page
Investigating the synergistic effect of pomegranate (Punica granatum L.) peel extract and quercetin on Calu-6 human lung carcinoma cells
Shima Osmani , Homa Mohseni-Kouchesfahani , Hanieh Jalali
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, I.R. Iran. , kouchesfehani@yahoo.com
Abstract:   (2411 Views)
Background: Lung cancer is one of the most common cancers. Herbal compounds have been considered for the treatment of cancer due to anticancer effects. Peel of Pomegranate (Punica granatum L.) has antioxidant and anticancer properties due to its phenolic compounds. Quercetin is a herbal flavonoid that can inhibit the proliferation of cancer cells. The present study compared the anti-proliferative effects of pomegranate’s fruit peel extract and quercetin on the calu-6 human lung carcinoma cell line and their co-effects in inhibiting the proliferation and migration of cancer cells.
Material and Methods: Calu-6 cell line was purchased from Pasteur institute and cultured in RPMI1640 medium containing 10% FBS and 1% antibiotic. To determine the survival rate of t-cells, MTT assay was performed. Type of cell death was determined by flow cytometry, and scratch test was used to determine the migration rate of the cells.
Results: The results showed that pomegranate fruit peel extract and quercetin inhibited the proliferation of lung cancer cells. Also, quercetin and pomegranate extract induced apoptosis in calu-6 cells individually and simultaneously so that the level of expression of annexin-V in quercetin and pomegranate peel extract was 18.20% and 15.59%, respectively, and increased by 25.51% during the co-treatment that difference was significant at P<0.05 The results of the scratch test also indicated that pomegranate peel extract and quercetin inhibited metastatic activity in calu-6 cells.
Conclusion: The results of this study showed that quercetin and pomegranate peel extract have anti-cancer effects and can be considered as a complementary medicine of lung cancer.
Keywords: Lung cancer, Quercetin, Pomegranate peel extract, Proliferation, Migration
Full-Text [PDF 609 kb]   (852 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2019/06/22 | Revised: 2020/02/22 | Accepted: 2019/11/3 | Published: 2020/02/12
1. Zappa C, Mousa SA. Non-small cell lung cancer: current treatment and future advances. Transl Lung Cancer Res 2016; 5(3): 288-300.
2. Sher T, Dy GK, Adjei AA. Small cell lung cancer. Mayo Clin Proc 2008; 83(3): 355-67.
3. Rayman MP. Selenium in cancer prevention: a review of the evidence and mechanism of action. Proc Nutr Soc. 2005; 64(4): 527-42.
4. Turati F, Rossi M, Pelucchi C, Levi F, La Vecchia C. Fruit and vegetables and cancer risk: a review of southern European studies. Br J Nutr. 2015; 113 Suppl 2: S102-110.
5. Sak K. Site-specific anticancer effects of dietary flavonoid quercetin. Nutr Cancer. 2014; 66(2): 177-93.
6. Sun J, Chu YF, Wu X, Liu RH. Antioxidant and antiproliferative activities of common fruits. J Agric Food Chem. 2002; 50(25): 7449-54.
7. Liu Z, Ren Z, Zhang J, Chuang CC, Kandaswamy E, Zhou T, et al. Role of ROS and Nutritional Antioxidants in Human Diseases. Front Physiol. 2018; 9: 477.
8. Shams Ardekani MR, Hajimahmoodi M, Oveisi MR, Sadeghi N, Jannat B, Ranjbar AM, et al. Comparative Antioxidant Activity and Total Flavonoid Content of Persian Pomegranate (Punica granatum L.) Cultivars. Iran J Pharm Res 2011; 10(3): 519-24.
9. Fedder MD, Jakobsen HB, Giversen I, Christensen LP, Parner ET, Fedder J. An extract of pomegranate fruit and galangal rhizome increases the numbers of motile sperm: a prospective, randomised, controlled, double-blinded trial. PLoS One. 2014; 9(9): e108532.
10. Sharma P, McClees SF, Afaq F. Pomegranate for Prevention and Treatment of Cancer: An Update. Molecules 2017; 22(1).
11. Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol. 2007; 109(2): 177-206 .
12. Ismail T, Sestili P, Akhtar S. Pomegranate peel and fruit extracts: a review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol. 2012; 143(2): 397-405.
13. Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, et al. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 2005; 16(6): 360-7.
14. Jeong JH, An JY, Kwon YT, Rhee JG, Lee YJ. Effects of low dose quercetin: cancer cell-specific inhibition of cell cycle progression. J Cell Biochem. 2009; 106(1): 73-82.
15. Fotakis G, Timbrell JA. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006; 160(2): 171-7.
16. Wallberg F, Tenev T, Meier P. Analysis of Apoptosis and Necroptosis by Fluorescence-Activated Cell Sorting. Cold Spring Harb Protoc. 2016; 2016(4): pdb prot087387.
17. Bobadilla AVP, Arevalo J, Sarro E, Byrne H M, Maini PK, Carraro T, et al. In vitro cell migration quantification method for scratch assays. J R Soc Interface. 2019; 16(151): 20180709.
18. Song B, Li J, Li J. Pomegranate peel extract polyphenols induced apoptosis in human hepatoma cells by mitochondrial pathway. Food Chem Toxicol 2016; 93: 158-66.
19. Asmaa MJ ,Ali AJ, Farid JM, Azman S. Growth inhibitory effects of crude pomegranate peel extract on chronic myeloid leukemia, K562 cells. Int J Appl Basic Med Res. 2015; 5(2): 100-5.
20. Dikmen M, Ozturk N, Ozturk Y. The antioxidant potency of Punica granatum L. Fruit peel reduces cell proliferation and induces apoptosis on breast cancer. J Med Food 2011; 14(12): 1638-46.
21. Dana N, Javanmard Sh, Rafiee L. Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line. Res Pharm Sci 2015; 10(2): 117-24.
22. Deng Y, Li Y, Yang F, Zeng A, Yang S, Luo Y, et al. The extract from Punica granatum (pomegranate) peel induces apoptosis and impairs metastasis in prostate cancer cells. Biomed Pharmacother 2017; 93: 976-84.
23. Lee SH, Lee EJ, Min KH, Hur GY, Lee SH, Lee SY, et al. Quercetin Enhances Chemosensitivity to Gemcitabine in Lung Cancer Cells by Inhibiting Heat Shock Protein 70 Expression. Clin Lung Cancer 2015; 16(6): e235-243.
24. Shan BE, Wang MX, Li RQ. Quercetin inhibit human SW480 colon cancer growth in association with inhibition of cyclin D1 and survivin expression through Wnt/beta-catenin signaling pathway. Cancer Invest. 2009; 27(6): 604-12.
25. Liu KC, Yen CY, Wu RS, Yang JS, Lu HF, Lu KW, et al. The roles of endoplasmic reticulum stress and mitochondrial apoptotic signaling pathway in quercetin-mediated cell death of human prostate cancer PC-3 cells. Environ Toxicol. 2014; 29(4): 428-39.
26. Kim JY, Kim EH, Park SS, Lim JH, Kwon TK, Choi KS. Quercetin sensitizes human hepatoma cells to TRAIL-induced apoptosis via Sp1-mediated DR5 up-regulation and proteasome-mediated c-FLIPS down-regulation. J Cell Biochem 2008; 105(6): 1386-98.
27. Jung YH, Heo J, Lee YJ, Kwon TK, Kim YH. Quercetin enhances TRAIL-induced apoptosis in prostate cancer cells via increased protein stability of death receptor 5. Life Sci 2010; 86(9-10): 351-7.
28. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, et al. Cell migration: integrating signals from front to back. Science. 2003; 302(5651): 1704-1709.
29. Liu X, Ji Q, Fan Z, Li Q. Cellular signaling pathways implicated in metastasis of colorectal cancer and the associated targeted agents. Future Oncol. 2015; 11(21): 2911-22.
30. Lai WW, Hsu SC, Chueh FS, Chen YY, Yang JS, Lin JP, et al. Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-kappaB and matrix metalloproteinase-2/-9 signaling pathways. Anticancer Res. 2013; 33(5): 1941-50.
31. Bagheri M, Fazli M, Saeednia S, Kor A, Ahmadiankia N. Pomegranate peel extract inhibits expression of beta-catenin, epithelial mesenchymal transition, and metastasis in triple negative breast cancer cells. Cell Mol Biol (Noisy-le-grand). 2018; 64(7): 86-91.
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Osmani S, Mohseni-Kouchesfahani H, Jalali H. Investigating the synergistic effect of pomegranate (Punica granatum L.) peel extract and quercetin on Calu-6 human lung carcinoma cells. Feyz 2019; 23 (6) :596-604
URL: http://feyz.kaums.ac.ir/article-1-3906-en.html

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Volume 23, Issue 6 (Bimonthly 2019) Back to browse issues page
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