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:: Volume 24, Issue 3 (Bimonthly 2020) ::
Feyz 2020, 24(3): 253-245 Back to browse issues page
Anti-anxiety effect of Alpha-pinene in comparison with Diazepam in adult male rats
Sadri Saeedipour , Maryam Rafieirad *
Department of Biology, Izeh Branch, Islamic Azad University, Izeh, I.R. Iran. , Rafieirad.m@gmail.com
Abstract:   (244 Views)
Background: Considering the side effects of anti- anxiety drugs, this study investigated the effect of alpha-pinene on anxiety behaviors compared with Diazepam in rats.
Materials and Methods In this study, 40 male Wistar rats were divided into 5 groups: control, sham (received alphapinene solvent), the groups received 2 and 4 mg/kg alphapinene, and the group that received diazepam 10 mg/kg. At the end of this period, anxiety was measured using elevated plus maze and concentrations of malondialdehyde, thiol and glutathione peroxidase, in hippocampal tissue. The results were analyzed using one-way ANOVA and Tukey tests.
Results: In the groups receiving 2 and 4 mg/kg doses of alpha-pinene significantly increased the percentage of open arm entry (P<0.001) and percentage of time spent in the open arm (P<0.05) (P<0.05). Compared to the control and diazepam groups, administration of alpha-pinene significantly decreased MDA concentration (P<0.001) and significant increase in thiol (P<0.01) (P<0.05) and glutathione peroxidase enzyme activity was evident (P<0.001).
Conclusion: Alpha-pinene showed a downward effect on anxiety responses in male rats, similar to anxiolytic effects of diazepam. Alpha-pinene may be potentiated by binding to the position of benzodiazepines in GABA A receptors and exerted its anti-anxiety effect with antioxidant properties.
Keywords: Anxiety, Alpha-pinene, Diazepam, Rat
Full-Text [PDF 320 kb]   (55 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/05/9 | Accepted: 2020/07/7 | Published: 2020/08/19
References
1. Vafaei A, Taherian AA, Sadegh Sobhaninejad M. Evaluation of interaction of Corticosterone and verapamil on anxiety related behavior in mice. Pharm Sci 2009; 15: 219-26. [in Persian]
2. Modaresi M, Basravi M, Sajadia I. Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice. Armaghane Danesh 2016; 20(10): 848-57. [in Persian]
3. Felgentreff F, Becker A, Meier B, Brattström A. Valerian extract characterized by high valerenic acid and low acetoxy valerenic acid contents demonstrates anxiolytic activity. Phymed 2012; 19(13): 1216-22.
4. Sandford J, Argyropoulos S, Nutt D. The psychobiology of anxiolytic drugs. Part 1 Basic Neurobiology. Pharmacol Ther 2000; 88(3): 197-212.
5. Rezaie A , Mosavi Gh, Ahmadizadeh Ch, Jafari B. Study of sedative, preanaesthetic and anti-anxiety effects of Rosa damascene herbal extract in comparison with diazepam in rat. TUMS 2011; 69(3): 179-84. [in Persian]
6. Johnston GA. Flavonoid nutraceuticals and ionotropic receptors for the inhibitory neurotransmitter GABA. Neurochem Int 2015; 89: 120-5.
7. Kessler A, Villmann C, Sahin-Nadeem H, Pischetsrieder M, Buettner A. GABAA receptor modulation by the volatile fractions of Sideritis species used as ‘Greek’ or ‘Turkish’ mountain tea, Flavour Frag J 2012; 27: 297–303.
8. Bouayed J, Rammal H, Soulimani R. Oxidative stress and anxiety: relationship and cellular pathways. Oxid Med Cell Longev 2009; 2(2): 63-7.
9. Ng F, Berk M, Dean O, Bush AI. Oxidative stress in psychiatric disorders: evidence base and therapeutic implications. IJNP 2008; 11(6): 851-76.
10. Khanzode S, Dakhale G, Khanzode S, Saoji A, Palasodkar R. Oxidative damage and major depression: The potential antioxidant action of selective serotonin-re-uptake inhibitors. Redox Rep 2003; 8(6): 365-70.
11. Jia Y, Zhu H, Leung SW. Comparative efficacy of selective serotonin reuptake inhibitors (SSRI) in treating major depressive disorder: a protocol for network meta-analysis of randomised controlled trials. BMJ Open 2016, 6(6): e010142.
12. Rajabi S, Noori S, Zal F, Jahanbazi Jahan-Abad A. Oxidative Stress and its Different Roles in Neurodegenerative Diseases. Neurosci J Shefaye Khatam 2017; 5(1): 73-86. [in Persian]
13. Morshedloo M, Ebadi A, Fatahi Moghaddam M, Yazdani D. Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran. J Med Plants 2012; 1(41): 218-26. [in Persian]
14. Yang Z, Wu N, Zu Y, Fu Y. Comparative anti-infectious bronchitis virus (IBV) activity of (-)-pinene: effect on nucleocapsid (N) protein. Molecules 2011; 16(2): 1044-54.
15. Samojlik I, Mijatović V, Petković S, Škrbić B, Božin B: The influence of essential oil of aniseed (Pimpinella anisum L.) on drug effects on the central nervous system. Fitoterapia 2012; 83(8): 1466-73.
16. Szabadics J, Erdélyi L. Pre- and postsynaptic effects of eugenol and related compounds on Helix pomatia L. Acta Biol Hung 2000; 51(2-4): 265-73.
17. Li XJ, Yang YJ, Li YS, Zhang W, Tang HB. α-Pinene, linalool, and 1-octanol contribute to the topical anti-inflammatory and analgesic activities of frankincense by inhibiting COX-2. J Ethnopharmacol 2015; 19: 22-6.
18. Fit N, Gheorghe R, Rapuntean S, Flore C, Cosmin Nadas G: Antibacterial Effect of Essential Vegetal Extracts on Staphylococcus aureus Compared to Antibiotics. Not Bot Horti Agrobo 2009; 37(2): 117-23.
19. Satou T, Kasuya H, Maeda K, Koike K. Daily Inhalation of α-Pinene in Mice: Effects on Behavior and Organ Accumulation. Phytother Res 2014 Sep; 28(9): 1284-7.
20. Goudarzi S, Rafieirad M. Evaluating the effect of α-pinene on motor activity, avoidance memory and lipid peroxidation in animal model of Parkinson disease in adult male rats. RJP 2017; 4(2): 53-63. [in Persian]
21. Yang H, Woo J, Pae AN, Um MY, Cho NC, Park KD, et al. alpha-Pinene, a Major Constituent of Pine Tree Oils, Enhances Non-Rapid Eye Movement Sleep in Mice through GABAA-benzodiazepine Receptors. Mol Pharmacol 2016; 90(5): 530-9.
22. Nozari K, Rafieirad M. Comparison of Alphapinene and Donpezil Effects on Passive Avoidance Memory in Adult Male Rats. Qom Univ Med Sci J 2019; 13(4): 1-10. [in Persian]
23. Rafieirad M, Abbaszadeh H. Pomegranate seed extract reduces ischemia induced anxiety in male rats. J Herbmed Pharmacol 2017; 6(2): 85-9.
24. Sharifi F, Rafieirad M, Sazegar H. Effects of Ferulago angulata Extract Against Oxidative Stress Induced by 6-hydroxydopamine in Rats. J Med Plants 2015; 1(53): 34-44.
25. Kasuya H, Iida S, Ono K, Satou T, Koike K. Intracerebral Distribution of a-Pinene and the Anxiolytic-like Effect in Mice Following Inhaled Administration of Essential Oil from Chamaecyparis obtuse. Nat Prod Commun 2015; 10(8): 1479-82.
26. Kuloglu M, Atmaca M, Tezcan E, Gecici O, Tunckol H, Ustundag B. Antioxidant enzyme activities and malondialdehyde levels in patients with obsessivecompulsive disorder. Neuropsychobiol 2002; 46(1): 27-32.
27. Hovatta I, Tennant RS, Helton R, Marr RA, Singer O, Redwine JM, et al. Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature 2005; 438(7068): 662-6.
28. Rabiee Z, Davoodizadeh Z, Bijad E, Rafieian-kopaei M. Evaluation of the Effects of Grape Seed Oil on the Anxiety Level and Motor Coordination of Male Wistar Rats. JBUMS 2016; 18(6): 52-8. [in Persian]
29. Gungor Ö, Özdemir N. Analysis of Oxidative Stress Parameters Depend on Diazepam Application in Rat Serum and Tissue Samples. Chem Sci Trans 2014; 3(1): 389-95.
30. Ogueji E, Iheanacho S, Nwani Ch, Mbah Ch, Okeke O, Usman I. Toxicity of diazepam on lipid peroxidation, biochemical and oxidative stress indicators on liver and gill tissues of African catfish Clarias gariepinus. RJIF 2017; 5(3): 114-23.
31. Mohamadi-Pour V, Motaghi S, Jonaidi H, Mirtadjadini SM: Investigation of anxiolytic and hypnotic effects of hydroalcoholic extracts of Lemon Verbena in mice. Ijpp Phypha 2018; 2(3): 184-77. [in Persian]
32. Banitaba S M, Alaei H. Surveying the effect of clonidine (α2 agonist) and idazoxan (α2 antagonist) on morphine dependency in rat. Feyz 2004; 8(1): 26-32. [in Persian]
33. Feng J, Cai X, Zhao JH, Yan Z. Serotonin Receptors Modulate GABA A Receptor Channels through Activation of Anchored Protein Kinase C in Prefrontal Cortical Neurons. Neurosci 2001; 21(17): 6502-11.
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Saeedipour S, Rafieirad M. Anti-anxiety effect of Alpha-pinene in comparison with Diazepam in adult male rats. Feyz. 2020; 24 (3) :253-245
URL: http://feyz.kaums.ac.ir/article-1-3863-en.html


Volume 24, Issue 3 (Bimonthly 2020) Back to browse issues page
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