:: Volume 23, Issue 5 (Bimonthly 2019) ::
Feyz 2019, 23(5): 485-494 Back to browse issues page
The effect of eight weeks high intensity interval training and crocin consumption on oxidative stress of liver tissue in male rats subjected to chronic doxorubicin injection
Mahraz Moradi , Saeid Shakerian , Masoud Nikbakht
Department of Exercise Physiology, Factuly of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, I.R. Iran. , sashakeryan@gmail.com
Abstract:   (3272 Views)
Background: Doxorubicin (Dox) is an anthracycline antibiotic that is widely used as a chemotherapy agent. However, the benefit of this agent is limited due to its side effects. The purpose of this study was to investigate the effect of high intensity interval training and crocin consumption on oxidative stress in liver tissue of male rats under chronic induction of doxorubicin.
Materials and Methods: In the present experimental study, 40 Wistar male rats were placed in groups: Healthy control (saline), doxorubicin (control) (2 mg/kg in 7 doses), doxorubicin and crocin (2mg/kg), doxorubicin training, and doxorubicin, crocin and training. The training groups ran in 80-90% of maximum speed for 8 weeks, 5 days a week, with 2-minute intervals. The level of malondialdehyde, activity of superoxide dismutase and catalase enzymes in liver tissue was measured.
Results: Doxorubicin significantly increased malondialdehyde levels and reduced the activity of superoxide dismutase and catalase in the liver tissue in the groups receiving doxorubicin compared to the healthy control group (P<0.001). Use of all three interventions caused a significant decrease in malondialdehyde level (P<0.001) and a significant increase in superoxide dismutase and catalase activity as a result of exercise (both enzymes, P=0.001), crocin (P=0.002 and P=0.001) and combination of exercise and crocin (both enzymes, P=0.001) compared to the control group of doxorubicin. Also, the combined effect of exercise and crocin was better than the effect of each alone (P<0.001).
Conclusion: It seems that regular high intensity interval training, supplementation of crocin, or a combination of these two, can reduce the toxicity of doxorubicin by reducing oxidative stress.
Keywords: Oxidative stress, High interval training Doxorubicin, Liver, Crocin
Full-Text [PDF 338 kb]   (1207 Downloads)    
Type of Study: Applicable | Subject: medicine, paraclinic
Received: 2019/04/27 | Revised: 2020/05/11 | Accepted: 2019/09/2 | Published: 2019/12/16
References
1. Siegel RL, Miller KD, Jemal A. Cancer statistics. CA Cancer J Clin 2015; 65(1): 5-29.
2. Li M, Xiong Z-G. Ion channels as targets for cancer therapy. Int J Physiol Pathophysiol Pharmacol 2011; 3(2): 156-166.
3. Marques-Aleixo I, Santos-Alves E, Balça M, Moreira P, Oliveira P, Magalhães J, et al. Physical exercise mitigates doxorubicin-induced brain cortex and cerebellum mitochondrial alterations and cellular quality control signaling. Mitochondrion 2016; 26: 43-57.
4. Mokni M, Hamlaoui S, Kadri S, Limam F, Amri M, Marzouki L, et al. Efficacy of grape seed and skin extract against doxorubicin-induced oxidative stress in rat liver. Pak J Pharm Sci 2015; 28(6): 1971-8.
5. Ascensão A, Lumini-Oliveira J, Machado NG, Ferreira RM, Gonçalves IO, Moreira AC, et al. Acute exercise protects against calcium-induced cardiac mitochondrial permeability transition pore opening in doxorubicin-treated rats. Clin Sci (Lond) 2011; 120(1): 37-49.
6. Kumral A, Giriş M, Soluk-Tekkeşin M, Olgac V, Doğru-Abbasoğlu S, Türkoğlu Ü, et al. Beneficial effects of carnosine and carnosine plus vitamin E treatments on doxorubicin-induced oxidative stress and cardiac, hepatic, and renal toxicity in rats. Hum Exp Toxicol 2016; 35(6): 635-43.
7. Saritaş N, Uyanik F, Hamurcu Z. Effects of acute twelve minute run test on oxidative stress and antioxidant enzyme activities. Afr J Pharm Pharmacol 2011; 5(9): 1218-22.
8. Abdollahi M, Ranjbar A, Shadnia S, Nikfar S, Rezaiee A. Pesticides and oxidative stress: a review. Med Sci Monit 2004; 10(6): 141-147. [in Persian]
9. Tuter G, Kurti S, Serdar M. Interleukin – 1beta and thiobarbitoric reactive substance (TBARS) levels after phase I periodontal therapy in patients with chronic periodontitis. J Periodontol 2001; 72: 883-8.
10. Das J, Ghosh J, Manna P, Sil PC. Taurine suppresses doxorubicin-triggered oxidative stress and cardiac apoptosis in rat via up-regulation of PI3-K/Akt and inhibition of p53, p38-JNK. Biochem Pharmacol 2011; 81(7): 891-909.
11. Indu R, Azhar T, Nair A, Nair CKK. Amelioration of doxorubicin induced cardio-and hepato-toxicity by carotenoids. J Cancer Res Ther 2014; 10(1): 62-7.
12. Heck SO, Fulco BC, Quines CB, Oliveira CE, Leite MR, Cechella JL, et al. Combined Therapy With Swimming Exercise and a Diet Supplemented With Diphenyl Diselenide Is Effective Against Age‐Related Changes in the Hepatic Metabolism of Rats. J Cell Biochem 2017; 118(6): 1574-82.
13. Peng CC, Chen K-C, Hsieh C-L, Peng RY. Swimming exercise prevents fibrogenesis in chronic kidney disease by inhibiting the myofibroblast transdifferentiation. PLoS One 2012; 7(6): e37388.
14. Chicco AJ, Hydock DS, Schneider CM, Hayward R. Low intensity exercise training during doxorubicin treatment protects against cardiotoxicity. J Appl Physiol 2006; 100 (2): 519-27.
15. Lambertucci RH, Levada-Pires AC, Rossoni LV, Curi R, Pithon-Curi TC. Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats. Mech ageing Dev 2006; 128: 267-75.
16. Gibala MJ, Little JP, MacDonald MJ, Hawley JA. Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. J Physiol 2012; 590 (5): 1077-84.
17. Freyssin C, Verkindt C, Prieur F, Benaich P, Maunier S, Blanc P. Cardiac rehabilitation in chronic heart failure: effect of an 8-week, high-intensity interval training versus continuous training. Arch Phys Med Rehabil 2012; 93(8): 1359-64.
18. Hakkakdokht E, Salami F, Rajabi H, M. H. The effect of aerobic exercise and vitamin E and C supplementation on GSH and antioxidative enzymes (GPX and SOD) in pregnant rats. J Olympic 2011; 19(3): 47-56. [in Persian]
19. Songstad NT, Kaspersen K-HF, Hafstad AD, Basnet P, Ytrehus K, Acharya G. Effects of high intensity interval training on pregnant rats, and the placenta, heart and liver of their fetuses. PloS One 2015; 10(11): e0143095.
20. Razavi BM, Hosseinzadeh H, Movassaghi AR, Imenshahidi M, Abnous K. Protective effect of crocin on diazinon induced cardiotoxicity in rats in subchronic exposure. Chem Biol Interact 2013; 203(3): 547-55. [in Persian]
21. Qi Y, Chen L, Zhang L, Liu WB, Chen XY, Yang XG. Crocin prevents retinal ischaemia/reperfusion injury-induced apoptosis in retinal ganglion cells through the PI3K/AKT signalling pathway. Exp Eye Res 2013; 107: 44-51.
22. Bathaie SZ, Mousavi SZ. New applications and mechanisms of action of saffron and its important ingredients. Crit Rev Food Sci Nutr 2010; 50(8): 761-86. [in Persian]
23. Goyal S, Arora S, Sharma A, Joshi S, Ray R, Bhatia J, et al. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomed 2010; 17(3-4): 227-32.
24. Elsherbiny NM, Salama MF, Said E, El-Sherbiny M, Al-Gayyar MM. Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-regulation of inflammatory and apoptic pathways. Chem Biol Interact 2016; 247: 39-48.
25. Rezaei R, Nurshahi M, Bigdeli MR, Khodagoli F, Haghparast A. Effect of eight weeks of continuous and periodic aerobic training on VEGF-A and VEGFR-2 levels of male brain Wistar rats. J Sports Physiol Phys Activ 2015; 16: 1213-21. [in Persian]
26. Marques-Aleixo I, Santos-Alves E, Mariani D, Rizo-Roca D, Padrão AI, Rocha-Rodrigues S, et al. Physical exercise prior and during treatment reduces sub-chronic doxorubicin-induced mitochondrial toxicity and oxidative stress. Mitochondrion 2015; 20: 22-33.
27. Ashrafi J, Dabidi Roshan V, Zolfagharzadeh F. Tissue toxicity induced by doxorubicin in rats: protective role of aerobic regular exercise. J Urmia Med Sci 2014; 25(4): 353-62. [in Persian]
28. Sakr SA, Mahran HA, Lamfon HA. Protective effect of ginger (Zingiber officinale) on adriamycin-induced hepatotoxicity in albino rats. J Med Plants Res 2011; 5(1): 133-40.
29. Kalender Y, Yel M, Kalender S. Doxorubicin hepatotoxicity and hepatic free radical metabolism in rats. The effects of vitamin E and catechin. Toxicology 2005; 209: 39-45.
30. Yagmurca M, Bas O, Mollaoglu H, Sahin O, Nacar A, Karaman O, et al. Protective effects of erdosteine on doxorubicin-induced hepatotoxicity in rats. Arch Med Res 2007; 38: 380-5.
31. Chatterjee K, Zhang J, Honbo N, Joel SK. Doxorubicin Cardiomyopathy. Cardiology 2010; 115: 155-62.
32. Öz E, İlhan MN. Effects of melatonin in reducing the toxic effects of doxorubicin. Mol Cell Biochem 2006; 286: 11–5.
33. Kavazis AN, Smuder AJ, Min K, Tümer N, Powers SK. Short-term exercise training protects against doxorubicin-induced cardiac mitochondrial damage independent of HSP72. Am J Physiol Heart Circ Physiol 2010; 299: H1515–24.
34. Tokarska-Schlattner M, Dolder M, Gerber I, Speer O, Wallimann T, Schlattner U. Reduced creatinestimulated respiration in doxorubicin challenged mitochondria: particular sensitivity of the heart. Biochim Biophys Acta 2007; 1767: 1276-1284.
35. Eder A, Arriaga E. Capillary electrophoresis monitors enhancement in subcellular reactive oxygen species production upon treatment with doxorubicin. Chem Res Toxicol 2006; 19: 1151-9.
36. Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Mol Med Rep 2015; 12(2): 2374-82.
37. Bafghi AF, Homaee H M, MA. A. Effects of high intensity interval training and curcumin supplement on antioxidant enzyme in heart tissue of diabetic rats. IJDO 2017; 8(3): 135-41. [in Persian]
38. Zolfagharzadeh F, Dabidi-Roshan V, Hajizadeh Moghaddam A. Pretreatment effect of three and six weeks aerobic exercise on acute doxorubicin-induced hepatic stress. Modern Olympic 2015; 1(2): 117-28. [in Persian]
39. El-Sayed EM, El-azeem ASA, Afify AA, Shabana MH, Ahmed HH. Cardioprotective effects of Curcuma longa L. extracts against doxorubicin-induced cardiotoxicity in rats. J Med Plants Res 2011; 5(17): 4049-58.
40. Díaz A, Loewen PC, Fita I, Carpena X. Thirty years of heme catalases structural biology. Arch Biochem Biophys 2012; 525 (2): 102-10.
41. Alfonso-Prieto M, Vidossich P, Rovira C. The reaction mechanisms of heme catalases: an atomistic view by ab initio molecular dynamics. Arch Biochem Biophys 2012; 525(2): 121-30.
42. Gedik S, Erdemli ME, Gul M, Yigitcan B, Bag HG, Aksungur Z, et al. Hepatoprotective effects of crocin on biochemical and histopathological alterations following acrylamide-induced liver injury in Wistar rats. Biomed Pharmacother 2017; 95: 764-70.
43. Salahshoor MR. Protective effect of crocin on liver toxicity induced by morphine. Res Pharm Sci 2016; 11(2): 120-9. [in Persian]
44. Vakili A, Eianali MR, Bandegi AR. The protective effects of Saffron against the oxidative damage in a transient model of focal cerebral ischemia in rats. TUMS 2011: 405-12. [in Persian]
45. Khanmohammadi R, Azarbaijani MA, Piri M, L. K. The effect of high intensity training and crocin on oxidative stress in male rats subjected to doxorubicin induction. Armaghane-danesh 2019; 23(6): 694-708. [in Persian]
46. Ghorbanzadeh V, Mohammadi M, Mohaddes G, Dariushnejad H, Chodari L, Mohammadi S. Protective effect of crocin and voluntary exercise against oxidative stress in the heart of high-fat diet-induced type 2 diabetic rats. Physiol Int 2016; 103(4): 459-68. [in Persian]


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Volume 23, Issue 5 (Bimonthly 2019) Back to browse issues page