[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 6 (Bimonthly 2019) ::
Feyz 2019, 23(6): 627-636 Back to browse issues page
The effect of endurance training and berberine chloride consumption on antioxidant enzymes in diabetic male Wistar rats heart tissue
Elham Farhadfar , Naser Behpour * , Mohammadali Azarbaeijani , Ali Moradi
Department of Physical Education, Razi University, Faculty of Physical Education and Sport Sciences, Kermanshah, I.R. Iran. , n_behpoor@yahoo.com
Abstract:   (1897 Views)
Background: Oxidative stress is involved in the pathogenesis of many diseases. Oxidative stress and antioxidant imbalance play a major role in the outbreak and development of diseases. This study aimed to investigate the effect of four weeks of endurance training and consumption of berberine chloride on cardiac tissue antioxidant enzymes in diabetic male Wistar rats.
Materials and Methods: In this experimental study, 40 adult male Wistar rats weighing approximately 200-250 g were randomly divided into 5 groups including healthy control group (without STZ injection, medication and exercise), diabetic control group (with STZ injection) , Diabetic Experimental Group+ Physical Exercise, Diabetic Experimental Group+ Berberine Intake (50 mg/kg), Diabetic Experimental Group+Physical Exercise+Berberine Intake. 24 hours after the last exercise session, the animals were isolated by intraperitoneal injection of ketamine anesthetic and heart tissue to measure the changes of the enzyme superoxide dismutase, catalase and glutathione peroxidase. Paired t-test and ANOVA were used to analyze the data using SPSS software (version 22) and at level α=0.05.
Results: The results showed that there was a significant difference in the level of CAT, SOD and GPX activity in the diabetic group after the use of berburn supplementation and endurance training (P=0.001). Results showed that there was a significant difference between the amount of exercise effect in the diabetic+training+berberine group with diabetic+training group and diabetic+berbine group.
Conclusion: The results of this study showed that endurance training can have a significant and effective effect on the activity of anti oxidant enzymes in the heart tissue of diabetic rats.
Keywords: Diabetes mellitus, Superoxide dismutase, Catalase, Glutathione peroxidase, Berberine chloride
Full-Text [PDF 317 kb]   (857 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/03/11 | Accepted: 2019/11/18 | Published: 2020/02/12
1. McLeay Y, Stannard S, Houltham S, Starck C. Dietary thiols in exercise: oxidative stress defence, exercise performance, and adaptation. J Int Soc Sports Nutr 2017; 14(1): 12-27.
2. Li S, Tan H, Wang N, Zhang Z, Lao L, Wong . The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int J Mol Sci 2015, 16: 26087–124.
3. Khani M, Motamedi P, Dehkhoda M, Karimi P. Effect of thyme extract supplementation on lipid peroxidation, antioxidant capacity, PGC-1α content and endurance exercise performance in rats. J Int Soc Sports Nutr 2017; 14(1): 11-24. [In Persian]
4. Fattahi B, Matin H, Azarbayjani M. Effects of High Intensity Interval training and curcumin supplement on antioxidant enzyme in heart tissue of diabetic rats. Iran J Diabetes Obesity 2016; 8(3). [In Persian]
5. Khoharo H, Almani S, Qureshi F, Zaffar S. Free Radical Scavenging Activity of Berberine in Acetaminophen Induced Liver Injury. Int J Surg Med 2017; 3(1): 27-36. [In Persian]
6. Pitocco D, Zaccardi F, Di Stasio E, Romitelli F, Santini SA, Zuppi C, et al. Oxidative stress, nitric oxide, and diabetes. Rev Diabetic Stud 2010; 7: 15-29.
7. Cicero AF, Baggioni A. Berberine and Its Role in Chronic Disease. Adv Exp Med Biol 2016; 928: 27-45.
8. Derosa G, Maffioli P, Cicero A. Berberine on metabolic and cardiovascular risk factors: an analysis from preclinical evidences to clinical trials. Expert Opin Biol Ther 2012; 12(8): 1113-24.
9. Singh A, Duggal S, Kuar N, Singh J. Berberin: Alkaloid with spectrum of pharmacological activities. J Natural Products 2010; 3: 64-75.
10. Ashraf H, Khaneshi F, Rafiee Raki F, Nejati V. Evaluation of Aqueous Extract of Berberis Integerrima Root on the Testis Tissue and Testosterone Levels in Stereptozotocine [STZ] Induced Diabetic Rats. Qom Univ Med Sci J 2013; 7(4): 28-35. [in Persian]
11. Kalalian Moghadam H, Baloch Nejad Mojarad P, Roghani M, khaksari M, Norouzi P, Fazli M, et al. The effect of berberine chloride on oxidative stress in hippocampus of streptozotocin-diabetic rats. SJIMU 2014; 22(4): 123-31. [in Persian]
12. Bezheh N, Mahbot Moghadam T, Shahin M. The Effect of Short-Term Supplementation of Barberry Extract on Glazymic Indices in Patients with Type 2 Diabetes Following an Aerobic Exercise. Iran J Pharm Res 2015; 7(3): 503-18. [in Persian]
13. Singh A, Duggal S, Kuar N, Singh J. Berberin: Alkaloid with spectrum of pharmacological activities. J Natural Products 2010; 3: 64-75.
14. Cicero AF, Baggioni A. Berberine and Its Role in Chronic Disease. Adv Exp Med Biol 2016; 928: 27-45.
15. Mahmudzadeh T, Saghebjoo M, Seghatol Eslami A, Hedayati M. Effect of aerobic training and pistacia atlantica extract consumption on pancreatic β-cells function in streptozotocin-induced diabetic rats. Iran J Diabetes Metabol 2014; 13(3): 252-62. [in Persian]
16. Farhangi N, Nazem F, Zehsaz F. Effect of Endurance Exercise on Antioxidant Enzyme Activities and Lipid Peroxidation in the Heart of the Streptozotocin-Induced Diabetic Rats. JSSU 2017; 24 (10): 798-809. [in Persian]
17. Majd A, Mehrabian S, Mostafai H, Rahmani H. Antioxidantand anticancer effect of aqueous extract of Berberis integerrima. J Biological Sci 2008; 1(1): 31-8. [in Persian]
18. Chae CH, Jung SL, An SH, Park BY, Wang SW, Cho IH, et al. Treadmill Exercise Improves Cognitive Function And Facilitates Nerve Growth Factor Signaling By Activating Mitogen-Activated Protein Kinase/Extracellular Signalregulated Kinase1/2 In The Streptozotocin-Induced Diabetic Rat Hippocampus. Neuroscience 2009; 75: 1665-73.
19. Lesslie M. Investigations of Biologically Relevant free Radicals Utilizing Novel Gas- Phase Analytical Techniques. [Dissertation]. Northern Illinois University. 2017.
20. Song NT, Kaspersen KHF, Hafstad AD, Basnet P, Ytrehus K, Acharya G. Effects of High Intensity Interval Training on Pregnant Rats, and the Placenta, Heart and Liver of their Fetuses. Plos One 2015; 10(11): E0143095.
21. Rauscher F, Sanders R, Watkins J. Effects of coenzyme Q10 treatment on antioxidant pathways in normal and streptozotocin-induced diabetic rats. J Biochem Mol Toxicol 2009; 15(1): 41-6.
22. Modiri MD, Tanideh F, Mohammadi N, Firouzmand M. The effects of short and middle times aerobic exercise with high intensities on ingredients antioxidant in female Sprague Dawley rats. Mashhad Univ Med Sci J 2014; 57(3): 587-95.
23. Azizbeigi K, Stannard SR, Atashak S, Mosalman Haghighi M. Antioxidant enzymes and oxidative stress adaptation to exercise training: Comparison of endurance, resistance, and concurrent training in untrained males. J Exerc Sci Fit 2014; 12(1): 1-6.
24. McCord J. The evolution of free radicals and oxidative stress. Am J Med 2000; 108: 652-9.
25. Alipour M, Salehi I, Ghadiri Soufi F. Effect of exercise on diabetes-induced oxidative stress in the rat hippocampus. Iran Red Crescent Med J 2012; 14(4): 222-8.
26. Teixeira-Lemos E, Nunes S, Teixeira F, Reis F. Regular physical exercise training assists in preventing type 2 diabetes development: focus on its antioxidant and anti-inflammatory properties. Cardiovasc Diabetol 2011; 28(3): 10-19.
27. Ozkaya Y, Agar A, Yargiçoglu P, Hacioglu G, Bilmen-Sarikçioglu S, Ozen I, et al. The effect of exercise on brain antioxidant status of diabetic rats. Diabetes Metab 2002; 28(5): 377-84.
28. Dong C, Hayashi K, Lee JB, Hayashi T. Characterization of structures and antiviral effects of polysaccharides from portulaca oleracea L. Chem Pharm Bull 2010; 58(4): 507-10.
29. Mirzaei B, Khosravi A, Rasoulian B, Mehrabani J. Comparing the effects of acute exhaustive exercise on cardiac troponin T serum and malondialdehyde of heart tissue response levels of endurance trained young rats. Diabetes Metab 2013; 5(9):16-24.
30. Mizobuchi N, Nakata H, Horimi T, Takahashi I. Serum superoxide dismutase (SOD) activity in diabetes mellitus. Rinsho Byori 1993; 41(6): 673-8.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Farhadfar E, Behpour N, Azarbaeijani M, Moradi A. The effect of endurance training and berberine chloride consumption on antioxidant enzymes in diabetic male Wistar rats heart tissue. Feyz 2019; 23 (6) :627-636
URL: http://feyz.kaums.ac.ir/article-1-3838-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 23, Issue 6 (Bimonthly 2019) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4570